胶原蛋白肽复合配方对去势大鼠骨质疏松的防治研究

目的评价胶原蛋白肽、植物雌激素染料木素和大豆甙元、柠檬酸钙联合防治去势大鼠骨质疏松的作用。方法 50只平均体重250g的三月龄SD雌性大鼠,随机分为5组,包括假手术组、去势对照组、雌激素组以及配方高剂量及低剂量组。给药12周,测定受试大鼠骨密度、Mi—cro—CT以及骨生物力学等。结果配方高剂量组与去势对照组相比股骨、股骨头以第四腰椎骨骨密度均有显著性的提高(P<0.05),并且比单独应用雌二醇效果更加明显。通过Micm—CT测得给药后受试大鼠的骨微结构相比去势对照组在骨容积率、骨小梁数量及骨小梁厚度上均有改善。给药组股骨的最大载荷与去势对照组相比有升高的趋势。结论高剂量的植物雌激素、胶原蛋白肽与柠檬酸钙联合应用能够改善卵巢去势大鼠的骨密度、骨微结构以及骨生物力学性质,对临床应用具有指导作用。     

培哚普利对去势大鼠骨质疏松的影响

目的观察培哚普利对去势大鼠骨质疏松的影响。方法将大鼠随机分为5组,每组10只。经切除大鼠双侧卵巢建立骨质疏松动物模型(OVX组)后,低(LP组)、中(MP组)、高剂量培哚普利组(HP组)经灌胃分别按2、4、8 mg/(kg·d)给药(溶解于1 ml生理盐水),正常对照组(CON组)仅切除卵巢周围部分脂肪组织,CON与OVX组仅灌胃等体积(1 ml)的生理盐水,均仅给药1次。给药24周后经股动脉采血并处死大鼠,检测血清钙、磷及碱性磷酸酶(ALP)活性,取股骨检测骨密度及生物力学变化。结果与CON组比较,OVX组血清钙、磷、ALP活性及骨密度明显下降(P0.05)。与OVX组比较,MP及HP组大鼠的血清钙含量明显升高(P0.05);MP组大鼠的血清磷含量明显升高(P0.05);HP组大鼠的ALP活性明显升高(P0.05),MP及HP组大鼠的骨密度均明显升高(P0.05);MP组大鼠股骨的最大力学载荷明显升高(P0.05)。结论各剂量培哚普利对改善骨质疏松模型大鼠的骨密度及生物力学均有效果,以中剂量培哚普利改善效果最佳。     

橄榄多酚对卵巢切除大鼠骨密度和骨钙含量的影响

目的 利用卵巢切除大鼠模型,研究橄揽多酚对其骨密度和骨钙舍量的影响.方法 试验共设5组,假手术纽,高、中、低剂量组及溶剂对照组.观察包括生长发育/骨钙含量、骨密度等相关指标.结果 卵巢切除各组大鼠体重及增童显著大于假手术组各组之间右股骨干重差异无显著性在大鼠骨密度低下造模成功的前提下,试验末3个剂量组左股骨密度及中、高剂量组右股骨骨钙含量显著高于溶剂畸对照蛆.结论 橄榄多酚可显著增加卵巢切除大鼠股骨密度及骨钙含量.     

PC对TFA染毒大鼠肝肾功能的影响

目的探讨原花青素(PC)对反式脂肪酸(TFA)致SD大鼠肝脏、肾脏损伤的保护作用。方法 SD大鼠30只随机分为5组,每组6只,实验8周后取血,测定肝肾功能。结果与对照组比较,TFA组、低、中剂量PC组大鼠ALT、AST、BUN、CRE含量均升高及UA下降,P 0.05。与TFA组比较,各组大鼠ALT(低、中、高剂量PC组)、AST(中、高剂量PC组)、BUN(高剂量PC组)、CRE(中、高剂量PC组)含量升高及UA(中、高剂量PC组)下降,P 0.05。结论原花青素对反式脂肪酸致SD大鼠肝肾功能损伤具有一定的修复作用。     

依达拉奉对大鼠脑缺血再灌注损伤的保护

目的探讨依达拉奉对大鼠脑缺血再灌注损伤的影响和作用机制。方法制备SD大鼠MCAO模型,梗死2h后再灌注,并分为假手术组、生理盐水(NS)组、依达拉奉低剂量组及依达拉奉高剂量组,再灌注24h后检测各组脑组织SOD、MDA浓度。结果与假手术组相比,NS组SOD活性降低、MDA含量增加,有显著性差异;与NS组相比,依达拉奉低剂量与高剂量组SOD活性增高、MDA含量降低,且低剂量组与高剂量组相比有显著性差异。结论依达拉奉对脑缺血再灌注损伤有保护作用。     

95．7％高效氟吡甲禾灵原药对Wistar大鼠的13周饲喂毒性

用95.7%高效氟吡甲禾灵原药对Wistar大鼠进行了13周喂饲毒性实验,实验设0、40(低)、200(中)、1000(高)mg/kg饲料4个剂量组,每组雌、雄鼠均为10只.实验结果表明:雄鼠中、高剂量组和雌鼠高剂量组的体重增长受到抑制;雄鼠低、中、高剂量组肝脏、睾丸的相对重最增高,雌鼠中、高剂量组动物肝脏相对重量增高;雄鼠低、中、高剂量组均出现睾丸无精子或精子形成低下、单个肝细胞坏死、小叶中心区肝细胞肿大等症状,而雌鼠中、高剂量组观察到单个肝细胞坏死,小叶中心区肝细胞肿大等症状;雄鼠低、中、高剂量组和雌鼠中、高剂量组动物碱性磷酸酶(ALP)水平增高,雌鼠和雄鼠的中、高剂量组动物丙氨酸氰基转氨酶(ALT)水平均增高,雄鼠高剂毋组总胆同醇水平增高,与对照相比差异显著.根据实验结果,在实验条件下,95.7%高效氟吡甲禾灵原药最大无作用剂量为雄性小于(3.01±0.31)mg/kg·d),雌性(3.10±0.18)mg/(kg·d),靶器官为肝脏和雄性动物的睾丸.     

番茄红素对高血脂大鼠的降血脂作用研究

研究不同剂量的番茄红素对高脂大鼠血脂的影响。将大鼠,分为6组分别为低、中、高剂量组、空白对照组、鱼油对照组和空白组,参照2012年5月国家食品药品监督管理局发布的国食药监保化[2012]107号中辅助降血脂功能的评价方法,根据辅助降血脂的检验方法对番茄红素低、中、高剂量组、鱼油对照组、空白对照组进行建模。高剂量与空白组大鼠的TC、TG、HDL-C、LDL-C的含量与空白对照组比较具有显著的差异(P0.01);中剂量与空白组大鼠的TC、TG、HDL-C、LDL-C的含量与空白对照组比较具有的差异性(P0.05);低剂量与空白组大鼠的TC、TG、HDL-C、LDL-C的含量与空白对照组比较无显著的差异性,鱼油对照组与中剂量组降血脂作用对比无明显差异性。番茄红素具有辅助降血脂的功效。     

根皮苷降尿酸及防治急性痛风性关节炎作用研究

以根皮苷为研究对象,探究根皮苷对大鼠高尿酸血症降尿酸作用及急性痛风性关节炎防治作用。方法:(1)采用腺嘌呤和乙胺丁醇联合建立高尿酸模型,设置正常对照组、模型组、别嘌呤组、根皮苷高、中、低剂量组,分别给予不同剂量根皮苷,检测各组血清中尿酸(UA)、尿素氮(BUN)、肌酐(Cr)及黄嘌呤氧化酶(XOD)水平;(2)采用目前公认急性型痛风性关节造模方法,在踝关节腔内注射尿酸钠晶体造模成功后,设置正常对照组、模型组、阳性对照组、根皮苷高、中、低剂量组,分别给予不同剂量根皮苷,检测各组IL-1β,IL-8,TNF-α的表达水平,观察大鼠踝关节组织HE染色病理切片。结果表明:(1)根皮苷高、中剂量组与模型组相比UA值呈显著差异(P0.05);别嘌呤组和根皮苷高、中剂量组对高尿酸血症大鼠肝脏XOD活性均有一定抑制作用,其抑制程度与其相同剂量组对血清尿酸的抑制程度基本一致,无统计学意义(P0.05);别嘌呤组、根皮苷高、中剂量组BUN、Cr均低于模型组(P0.01),说明与别嘌呤组药物对肾组织的毒性相比,中高剂量组很小或不会造成肾损伤。(2)根皮苷高、中剂量组对与模型组相比IL-1β,IL-8,TNF-α的水平显著降低(P0.05),观察大鼠踝关节组织HE染色病理切片,根皮苷高、中剂量组大鼠踝关节组织炎细胞浸润与模型组相比较减少。因此,可推论根皮苷可显著降低高尿酸血症大鼠尿酸水平,其作用机制与别嘌醇相似,同时对大鼠肝肾具有保护作用,且根皮苷对大鼠踝关节组织炎症也具有防治作用,其作用机制与IL-1β,IL-8,TNF-α因子具有相关性。     

大豆膳食纤维对糖尿病大鼠模型血糖的影响

目的探讨大豆膳食纤维对糖尿病大鼠模型血糖的影响。方法腹腔注射链脲佐菌素溶液(150 mg/kg)构建糖尿病大鼠模型。建模成功后,灌胃给予低、中、高大豆膳食纤维溶液(1.35、2.70、5.40 g/kg),同时设对照组(灌胃给予10 mg/kg的格列本脲),空白组及模型组灌胃给予0.9%生理盐水,每日给药1次,连续给药42 d。测定各组大鼠体重、空腹血糖值、血清中糖化血清蛋白(glycosylated serum protein,GSP)、胰岛素(insulin,INS)、肝糖原(glycogen)含量及胰腺/体比,并取胰脏组织,制作HE染色病理学切片进行观察。结果与空白组比较,模型组大鼠体重、INS、glycogen及胰腺/体比明显下降(P0.05),空腹血糖值及GSP含量明显升高(P0.01),且胰腺组织损伤严重,胰岛萎缩,细胞水肿变形,细胞间隙存在出血点及炎性细胞浸润现象。与模型组比较,除低剂量大豆膳食纤维剂量组外,中、高剂量组大鼠体重及INS均明显升高(P0.01),GSP明显下降(P0.01);各剂量大豆膳食纤维剂量组及对照组大鼠空腹血糖值明显下降(P0.05),glycogen含量明显升高(P0.05);高剂量大豆膳食纤维剂量组及对照组大鼠胰腺/体比明显升高(P0.05);且各剂量组大鼠胰腺组织损伤程度明显减轻,胰岛细胞数目增多,且细胞水肿变形程度减轻。结论大豆膳食纤维可降低糖尿病血糖含量、修复氧化损伤,且可改善糖尿病对大鼠胰腺的损伤情况,改善效果与剂量呈正相关。     

Ⅰ型糖尿病模型大鼠在重复给药毒性试验中的应用

目的研究Ⅰ型糖尿病模型大鼠在精蛋白重组人胰岛素注射液重复给药毒性试验中的应用。方法使用链脲佐菌素(streptozocin,STZ)对健康SD大鼠造模,55 mg/kg,计算6个月后的存活率。取模型大鼠,分高、中、低剂量(30、12、5 U/kg)组和对照组,每组20只,雌雄各半,高、中、低剂量组给予精蛋白重组人胰岛素注射液,对照组给予精蛋白重组人胰岛素注射液辅料空白液,均经大鼠皮下注射,1次/d,连续4周,进行重复给药毒性试验,给药4周后,经大鼠腹主动脉采血,检测血清胰岛素含量、C肽含量、糖化血红蛋白含量以及血液生化学和血液细胞学相关指标,并进行组织病理学观察。结果模型大鼠血糖均高于16.7 mmol/L,建模成功率为91.7%,6个月存活率为82%,确定建模成功。重复给药毒性试验高剂量组大鼠死亡4只。与其他剂量组相比,给药结束后和恢复期结束后高剂量组大鼠血清中糖化血红蛋白降低(P0.05);与对照组相比,给药结束后,胰岛素、C肽含量升高(P0.05),恢复期结束后,高、中剂量组胰岛素、C肽含量升高(P0.05);与对照组相比,给药结束后,高剂量组雄鼠和雌鼠血清中白蛋白、血小板升高(P0.05),淋巴细胞数降低(P0.05),而雌鼠红细胞、白细胞减少(P0.05),其余血液细胞学指标未见明显差异。恢复期结束后,各剂量组血液生化学和血液细胞学指标均无差异,建模成功大鼠主要脏器均出现病理变化,重复给药毒性试验中各组大鼠各主要脏器在给药结束后和恢复期结束后也分别出现不同程度的病理变化。结论用STZ造模的Ⅰ型糖尿病大鼠对于精蛋白重组人胰岛素产生的低血糖反应具有较好的耐受性,接近于临床患者的病理生理特征,在使用精蛋白重组人胰岛素进行重复给药毒性试验中具有实际应用价值。     

Cumulative Effects of Strontium Ranelate and Impact Exercise on Bone Mass in Ovariectomized Rats

OBJECTIVE: To explore the effect of physical exercise (EXE), strontium ranelate (SR), or their combination on bone status in ovariectomized (OVX) rats. DESIGN: Sixty female Wistar rats were randomized to one of five groups: sham (Sh), OVX (O), OVX+EXE (OE), OVX+SR (OSR), and OVX+EXE+SR (OESR). Animals in EXE groups were subjected to 10 drops per day (45 cm in height); rats in SR groups received 625 mg/kg/day of SR, 5 days/week for 8 weeks. Bone mineral density (BMD) and bone mineral content (BMC, dual-energy X-ray absorptiometry (DXA)), mechanical strength of the left femur (three-point bending test), and femur microarchitecture of (micro-computed tomography imaging, microCT) analyses were performed to characterize biomechanical and trabecular/cortical structure. Bone remodeling, osteocyte apoptosis, and lipid content were evaluated by ELISA and immunofluorescence tests. RESULTS: In OVX rats, whole-body BMD, trabecular parameters, and osteocalcin (OCN) levels decreased, while weight, lean/fat mass, osteocyte apoptosis, and lipid content all increased. EXE after ovariectomy improved BMD and BMC, trabecular parameters, cross-sectional area (CSA), moment of inertia, and OCN levels while decreasing osteocyte apoptosis and lipid content. SR treatment increased BMD and BMC, trabecular parameters, CSA, stiffness, OCN, and alkaline phosphatase (ALP) levels. Furthermore, fat mass, N-telopeptide (NTX) level, osteocyte apoptosis, and lipid content significantly decreased. The combination of both EXE and SR improved bone parameters compared with EXE or SR alone. CONCLUSION: EXE and SR had positive and synergistic effects on bone formation and resorption.     

Effect of cistanches herba aqueous extract on bone loss in ovariectomized rat

To assess the ability of traditional Chinese medicine Cistanches Herba extract (CHE) to prevent bone loss in the ovariectomized (OVX) rat, Cistanches Herba extract (CHE) was administered intragastrically to the rats. Female rats were anesthetized with pentobarbital sodium (40 mg kg(-1), i.p.), and their ovaries were removed bilaterally. The rats in the sham-operated group were anesthetized, laparotomized, and sutured without removing their ovaries. After 1 week of recovery from surgery, the OVX rats were randomly divided into three groups and orally treated with H(2)O (OVX group) or CHE (100 or 200 mg kg(-1) daily) for 3 months. The sham-operated group (n = 8) was orally treated with H(2)O. After 3 months, the total body bone mineral density (BMD), bone mineral content (BMC), Bone biomechanical index, blood mineral levels and blood antioxidant enzymes activities were examined in sham-operated, ovariectomized and Cistanches Herba extract treated rats. Results showed that Cistanches Herba extract treatment significantly dose-dependently enhanced bone mineral density (BMD), bone mineral content (BMC), maximum load, displacement at maximum load, stress at maximum load, load at auto break, displacement at auto break, and stress at auto break, and blood antioxidant enzymes activities, decreased blood Ca, Zn and Cu levels compared to the OVX group. This experiment demonstrates that the administration of Cistanches Herba extract to ovariectomized rats reverses bone loss and prevents osteoporosis.     

Studies on the lipids of sheep red blood cells. II. The incorporation of phosphorus into phospholipids of HK and LK cells

The incorporation of inorganic phosphate (as NaH₂PO₄) into the phospholipids of sheep red blood cells was studied in vitro in blood samples from five highpotassium (HK) and five low-potassium (LK) sheep. The erythrocytes from HK sheep incorporated more activity in 4 hr than those from the LK sheep. However no activity was incorporated into the major phospholipids of the cells (phosphatidyl ethanolamine, phosphatidyl serine, and sphingomyelin) of either group. The phosphatidic acid fraction was labeled in both groups and to a significantly greater extent in the HK samples. However the highest activity in the phospholipid of sheep red-cells was located in three unknown compounds not previously detected. Their specific activities were the same in the HK and the LK samples although they were present in slightly larger amounts in the HK samples. In general, incorporation was at a rather low level, and from stoichiometric considerations it was concluded that the metabolism in the red-cell phospholipids could not be directly involved in the active transport of ions across the cell membrane. This work also confirmed a previous report that no quantitative differences exist among the major phospholipid classes in the two types of cells.     

重组胸腺素α1对流感疫苗的增效作用

目的探讨重组胸腺素α1(Recombinant human thymosinα1,rhTα1)对免疫低下小鼠接种流感疫苗后免疫应答效果的影响。方法将BALB/c小鼠随机分为5组:疫苗组、环磷酰胺+疫苗组、环磷酰胺+疫苗+rhTα1低浓度(0.2 mg/kg)组、环磷酰胺+疫苗+rhTα1中浓度(0.4 mg/kg)组、环磷酰胺+疫苗+rhTα1高浓度(0.8 mg/kg)组。经小鼠腹腔注射环磷酰胺,80 mg/kg,共3次,隔天给药;肌肉接种流感疫苗,1.8μg血凝素/只;皮下注射低、中、高浓度rhTα1,0.2 ml/只,每周2次,连续4周。动态监测各组小鼠血清血凝抑制(HI)抗体效价,并观察小鼠脾脏指数及脾脏形态学结构的变化。结果与疫苗组相比,环磷酰胺+疫苗组小鼠体内HI抗体效价降低,脾指数降低,脾脏组织病理形态退化;与环磷酰胺+疫苗组相比,环磷酰胺+疫苗+rh-Tα1各浓度组HI抗体效价升高,小鼠脾指数增加,脾脏组织病理形态明显改善。结论重组胸腺素α1可提高免疫低下小鼠流感疫苗接种的免疫效果,为寻找适合免疫低下人群的疫苗增强剂提供了有益的尝试。     

Alkaline Activation of Metakaolin: Effect of Calcium Hydroxide in the Products of Reaction

The alkali activation of metakaolin (MK) leads to the production of high-mechanical-performance network-structure materials. Adding calcium hydroxide (Ca(OH)₂) to the raw MK produces a somewhat different reaction: a network structure and C-S-H gel form. In the present study, MK and (MK + Ca(OH)₂) mixes were activated with 5 M and 12 M NaOH solutions and cured at 45°C. A 5 M concentration, in the absence of Ca(OH)₂, did not produce MK activation within the test time. An activator concentration of 12 M resulted in complete activation and the formation of a network structure. When Ca(OH)₂ was present in the raw mix, a small amount of C-S-H gel formed.     

Is the Jaw Bone Micro-Structure Altered in Response to Osteoporosis and Bisphosphonate Treatment? A Micro-CT Analysis

The aim of the study was to quantify the micro-architectural changes of the jaw bone in response to ovariectomy, exposed or not to bisphosphonate treatment. A total of 47 Wistar rats were ovariectomized (OVX) or sham-operated (shOVX) and exposed to osteoporosis preventive treatment for eight weeks either with bisphosphonates (alendronate, ALN; group OVX-ALN) three days/week at a dose of 2 mg/kg or with saline solution (untreated control condition; group OVX). The bone morphometric parameters of the trabecular jaw bone were assessed using ex vivo micro-computed tomography. The regions of interest investigated in the maxilla were the inter-radicular septum of the second molar and the tuber. The regions quantified in the mandible included the three molar regions and the condyle. A one-way analysis of variance followed by pairwise comparison using Tukey’s HSD and the Games–Howell test was conducted to explore significant differences between the groups. In the maxilla, OVX decreased the bone volume in the inter-radicular septum of the second molar. Bisphosphonate treatment was able to prevent this deterioration of the jaw bone. The other investigated maxillary regions were not affected by (un)treated ovariectomy. In the mandible, OVX had a significant negative impact on the jaw bone in the buccal region of the first molar and the inter-radicular region of the third molar. Treatment with ALN was able to prevent this jaw bone loss. At the condyle site, OVX significantly deteriorated the trabecular connectivity and shape, whereas preventive bisphosphonate treatment showed a positive effect on this trabecular bone region. No significant results between the groups were observed for the remaining regions of interest. In summary, our results showed that the effects of ovariectomy-induced osteoporosis are manifested at selected jaw bone regions and that bisphosphonate treatment is capable to prevent these oral bone changes.     

不同钙含量粉煤灰合成沸石对污水中磷的去除

进行了以不同钙含量的粉煤灰为原料合成的3种沸石对污水中磷去除的试验，探讨了表面交换性阳离子种类、投加量以及磷浓度对除磷的影响。结果表明：最佳投加量为8g／L，磷去除率可达到70％以上合成沸石除磷能力均高于粉煤灰，钙饱和处理又可明显进一步提高粉煤灰合成沸石的除磷效果。粉煤灰合成沸石钙含量越高，对磷的去除率越高。因此，采用高钙粉煤灰为原料合成沸石并进行钙饱和处理，可以获得除磷能力强的合成沸石材料。     

Exercise and Zoledronic Acid on Lipid Profile and Bone Remodeling in Ovariectomized Rats: a Paradoxical Negative Association?

Exercise (EXE) and amino-bisphosphonates (BP) are both considered as useful strategies in the prevention of post-menopausal bone loss. Exercise reduces lipid levels, and BP may induce increase in high-density lipoprotein cholesterol (HDL-C). We hypothesized that combined effects of BP and exercise would produce a better improvement of lipid profile. We studied the specific and combined effects of zoledronic acid (Z) and EXE on lipid profile and bone remodeling in mature ovariectomized (OVX) rats. Six-month old female rats were randomly assigned to either a sham-ovx group (n = 12) or one of four OVX groups (n = 12): vehicle-treated sedentary (OVX); OVX + EXE (OVX-E, running on a treadmill for 12 weeks); OVX + Z (20 μg/kg, i.v.), (OVX-Z); OVX + Z+EXE (OVX-ZE). Total cholesterol (TC), HDL-C and bone remodeling markers were measured at baseline and at the end of the study. We demonstrated that both Z and EXE prevented the increase in bone resorption resulting from OVX, and individually improved the atherosclerotic risk index. Therapy with Z resulted in significant increase (39.00 ± 0.03 vs. 53.6 ± 0.01 mg/dl; +37.4%, P < 0.05) in serum concentration of HDL-C and a non significant decrease in TC (135.30 ± 0.03 vs. 144.80 ± 0.05 mg/dl; −5.8%) in the OVX-Z group compared to the OVX group. Post-menopausal women have elevated risk of CVD and bone resorption, hence, these data ultimately demonstrate (except for the elevated ratio in the combined group) that exercise and zoledronic acid are useful in minimizing the impact of these two processes in women and the combination of the two may be clinically relevant.     

胶原蛋白肽与柠檬酸钙联用抑制去卵巢大鼠骨丢失

胶原蛋白肽与柠檬酸钙是治疗骨质疏松最广泛应用的骨骼健康补充剂。目前,普遍的用药方式通常是只服用其中的一种,而胶原蛋白肽与柠檬酸钙联合服用,是否更有利于阻止骨质流失,还未见报道。本文采用胶原蛋白肽与柠檬酸钙联用,考察两者之间的协同作用对治疗去卵巢大鼠骨质流失的影响。经双能量X射线骨密度仪(DXA)和微机断层扫描检测(μCT)结果分析,结果表明:胶原蛋白肽与柠檬酸钙的协同作用,导致小鼠股骨密度增加,股骨远端微结构强度增强。由于去卵巢大鼠模型与女性绝经期骨质流失类似,因此本研究对治疗绝经期后女性的骨质酥松也具有潜在的指导意义。     

Dietary pseudopurpurin effects on bone mineral density and bone geometry architecture in rats

The objective of our study was to evaluate whether feeding pseudopurpurin affects bone mineral density and bone geometry architecture in rats. Pseudopurpurin was extracted, analyzed and purified using an HLPC-ESI-MS. Rats were given 0% and 0.5% pseudopurpurin powder in their diet. Femurs of rats were examined at 0.5, 1 and 2 months after pseudopurpurin feeding. Compared with rats in the group 0%, the bone mineral density, and the calcium, magnesium, zinc and manganese concentrations in the rats femur in the group 0.5% increased significantly at 1 month and 2 months after pseudopurpurin feeding. Analytical results of micro-computed tomography showed that the group 0.5% displayed an increase in the trabecular volume fraction, trabecular thickness and trabecular number of the distal femur at 1 and 2 months after pseudopurpurin feeding, and the mean thickness, inner perimeter, outer perimeter, and area of the femur diaphysis were significantly increased at 2 months after pseudopurpurin feeding compared with the group 0%. In parallel, the trabecular separation and structure model index of the distal femur were decreased, compared with the group 0% at 1 and 2 months after pseudopurpurin feeding. In the 0.5% and 0% groups, there was no damage to kidney and liver by histopathology analysis. The long-term feeding of pseudopurpurin is safe for rats. The feeding of 0.5% pseudopurpurin which has specific chemical affinities for calcium for bone improvement and level of bone mineral density, enhances the geometry architecture compared with the 0% group.