External Flow of an Acoustically Levitated Droplet

One of the major recent advances for experiments in containerless processing is acoustic levitation. Although there are a lot of previous studies for acoustic levitation, characteristic of external flow of an acoustically levitated droplet is not experimentally examined enough. In this study, external flow field has been observed by using high speed camera and Particle Image Velocimetry. In the case of any levitated droplet at a velocity antinode of standing wave, toroidal vortex are generated around levitated droplet. It is found that toroidal vortex around a levitated droplet is strongly affected by viscosity of levitated samples and input voltage. In terms of water droplet, as input voltage is decreased, location of toroidal vortex is moved from bottom to top of levitated samples.     

Internal Flow of Acoustically Levitated Droplet

Under the microgravity environment, products of new and high quality materials solidified into homogeneous crystal by under cooling solidification have been the subject of much interest. Manufacture of material under the microgravity environment can be performed more static than that under the normal gravity. Handling technology of molten metal is important for such processes to hold in the limit space. However, when a large levitated droplet exists in the limit space, internal flow can be appeared remarkably. Elucidation of the effect of the internal flow of the levitated droplet is required in order to establish the containerless processing for new material under the microgravity environment. In current research, the internal flow of a levitated droplet was investigated by Zhao et al. (J Acoust Soc Am 106:589–595, 1999a and 106:3289–3295, 1999b) and Trinh et al. (Phys Fluids 12(2):249–251, 2000). These studies were analyzed numerically and theoretically. However, experimental study about the internal flow of the levitated droplet is not enough. According to our study Abe et al. (Microgravity Sci Technol 19(3–4):33–34, 2007), the authors observed internal flow of the water and glycerol droplet in normal gravity environment. In the water droplet, which is a low viscosity fluid, internal flow of both left and right hand rotation was observed. On the other hand, in the glycerol droplet, which is a high viscosity fluid, only rigid body rotation was observed. This research measured only two dimensional flows. It is thought that internal flow in the water is not two-dimensional but three-dimensional flow. Then, in order to investigate a three-dimensional flow structure in levitated water droplet in detail, we try to measure the three-dimensional flow in the levitated droplet. In the present study, test fluid with different viscosity is levitated. And, multidimensional PIV measurement is conducted to investigate the internal flow structure in a levitated droplet. Stereo images at equatorial plane of a levitated droplet are observed for measuring the three-dimensional component of velocity in the levitated droplet. As a result, the velocity of z direction is observed in the water droplet. On the other hand, the v _z is hardly observed in the glycerol droplet. The three dimensional structures of water and glycerol are differed. The difference of such flow structure is supposed to be due to the influence of the viscosity.     

Effect of Laser Heating on Nonlinear Surface Deformation of Acoustically Levitated Droplet

Under the microgravity environment, new and high quality materials with a homogeneous crystal structure are expected to be manufactured by undercooling solidification, since the material manufacturing under the microgravity environment has no effect of gravity. However, the temperature change on the interface of the material in space is expected to affect on the material processing due to the changing of physical property corresponding temperature. The purpose of the present study is to investigate effect of the laser heating on surface deformation of large levitated droplet. A water droplet levitated in the acoustic standing wave is heated by YAG laser. In order to increase the water droplet temperature, rhodamine 6G is solved in it to achieve high absorbance of the laser. Droplet from 2.5 to 5.5 mm in diameter were levitated and heated. The deformation of the droplet interface has been observed by high speed video camera. We used the radiation thermometer for the measurement of the temperature of droplet surface. It is noticed that the heated droplet deforms with its resonance frequencies. The experimental result of shape oscillation makes it possible to simulate the oscillation.     

Precise Density Measurements for Electromagnetically Levitated Liquid Combined with Surface Oscillation Analysis

A new method is proposed for accurately measuring the densities of high-temperature liquids which involves analyzing the surface oscillations of levitated droplets. This method makes it easy to improve on the accuracy of density measurements obtained by using conventional electromagnetic levitation systems. In addition, the errors in density measurements made on the ground are further reduced by applying a static magnetic field to suppress surface oscillations in levitated liquid droplets. The magnetic field interacts with electrical currents in the levitated droplet, thereby generating a Lorentz force; this force suppresses flow within the liquid droplet. By combining both these methods, the scatter in density measurements for molten Si at temperatures in the range from 1,500 K to 1,900 K is reduced by an order of magnitude compared with previously reported data. Using this new method, the density of molten SiGe has been measured at temperatures from 1,350 K to 1,650 K.     

Study on Heat Transfer and Flow Characteristic Under Phase-Change Process of an Acoustically Levitated Droplet

Acoustic levitation is one of the levitation technique which is expected to be used for analytical chemistry and manufacturing new materials. Thus, it is important to gather the knowledge about acoustically levitated droplet. The purpose of this study is to investigate the heat transfer and flow behavior under phase change process of an acoustically levitated droplet. The following results were obtained from experiments. Evaporation process and external flow structure of the levitated droplet is visualized by a high speed camera and it is found that they differ by the type of fluid. Toroidal vortices are observed near the surface of the ethanol solution droplet. Heat transfer coefficient is estimated from the volume change and temperature gradient. It is substantially higher than that estimated by the existing experimental correlation.     

Mass spectrometry of acoustically levitated droplets

Containerless sample handling techniques such as acoustic levitation offer potential advantages for mass spectrometry, by eliminating surfaces where undesired adsorption/desorption processes can occur. In addition, they provide a unique opportunity to study fundamental aspects of the ionization process as well as phenomena occurring at the air-droplet interface. Realizing these advantages is contingent, however, upon being able to effectively interface levitated droplets with a mass spectrometer, a challenging task that is addressed in this report. We have employed a newly developed charge and matrix-assisted laser desorption/ionization (CALDI) technique to obtain mass spectra from a 5-microL acoustically levitated droplet containing peptides and an ionic matrix. A four-ring electrostatic lens is used in conjunction with a corona needle to produce bursts of corona ions and to direct those ions toward the droplet, resulting in droplet charging. Analyte ions are produced from the droplet by a 337-nm laser pulse and detected by an atmospheric sampling mass spectrometer. The ion generation and extraction cycle is repeated at 20 Hz, the maximum operating frequency of the laser employed. It is shown in delayed ion extraction experiments that both positive and negative ions are produced, behavior similar to that observed for atmospheric pressure matrix-assisted laser absorption/ionization. No ion signal is observed in the absence of droplet charging. It is likely, although not yet proven, that the role of the droplet charging is to increase the strength of the electric field at the surface of the droplet, reducing charge recombination after ion desorption.     

Numerical evaluation of droplet sizing based on the ratio of fluorescent and scattered light intensities (LIF/Mie technique)

The dependence of fluorescent and scattered light intensities from spherical droplets on droplet diameter was evaluated using Mie theory. The emphasis is on the evaluation of droplet sizing, based on the ratio of laser-induced fluorescence and scattered light intensities (LIF/Mie technique). A parametric study is presented, which includes the effects of scattering angle, the real part of the refractive index and the dye concentration in the liquid (determining the imaginary part of the refractive index). The assumption that the fluorescent and scattered light intensities are proportional to the volume and surface area of the droplets for accurate sizing measurements is not generally valid. More accurate sizing measurements can be performed with minimal dye concentration in the liquid and by collecting light at a scattering angle of 60° rather than the commonly used angle of 90°. Unfavorable to the sizing accuracy are oscillations of the scattered light intensity with droplet diameter that are profound at the sidescatter direction (90°) and for droplets with refractive indices around 1.4.     

Effect of Temperature Change on Interfacial Behavior of an Acoustically Levitated Droplet

Under the microgravity environment, new and high quality materials with a homogeneous crystal structure are expected to be manufactured by undercooling solidification, since the material manufacturing under the microgravity environment is more static than that under the normal gravity. However, the temperature change on the interface of the material in space can affect on the material processing. The purpose of the present study is to investigate effect of the temperature change of interface on the large levitated droplet interface. A water droplet levitated by the acoustic standing wave is heated by YAG laser. In order to heat the water droplet by the laser heating, rhodamine 6G is solved in it to achieve high absorbance of the laser. The droplet diameter is from 4 to 5.5 mm. The deformation of the droplet interface is observed by high speed video camera. The temperature of droplet is measured by the radiation thermometer. It is noticed that the larger droplet under the higher sound pressure tends to oscillate remarkably by the laser heating.     

Interfacial stability and internal flow of a levitated droplet

Under the microgravity environment, production of new and high quality material is expected. Large droplet is preferable for such a containerless processing in microgravity environment. There are a lot of previous studies for droplet levitation [1]. However, effect of surface instability and internal flow appear remarkable when the droplet becomes large. Elucidation of effect of surface instability and internal flow of the levitated droplet is required for the quality improvement of new material. The objective of present study is to clarify critical conditions of the occurrence of the internal flow and the surface instability. At first, the condition between the stable region and the unstable region of the droplet levitation was evaluated by using the existing critical Weber number theory. The experimental result agreed well with the theory. It was suggested that the stability of droplet can be evaluated by using the theory for the interfacial instability. Finally, two-dimensional visual measurement was conducted to investigate the internal flow structure in a levitated droplet. The effect of physical properties on the internal flow structure of the droplet is investigated by Particle Image Velocimetry (PIV) technique. As the result, it is indicated that the internal flow structure is affected by the physical property such as viscosity.     

On-line monitoring of airborne chemistry in levitated nanodroplets: in situ synthesis and application of SERS-active Ag-Sols for trace analysis by FT-Raman spectroscopy

We report a new strategy for on-line monitoring of chemical reactions in ultrasonically levitated, nanoliter-sized droplets by Raman spectroscopy. A flow-through microdispenser connected to an automated flow injection system was used to dose picoliter droplets into the node of an ultrasonic trap. Taking advantage of the flow-through characteristics of the microdispenser and the versatility of the automated flow system, a well-defined sequence of reagents could be injected via the microdispenser into the levitated droplet placed in the focus of the collection optics of the Fourier transform Raman spectrometer. In that way, chemical reactions could be carried out and monitored on-line. The developed system was used for fast, reproducible, in situ synthesis of a highly active surface enhanced Raman scattering (SERS) sol resulting from the reduction of silver nitrate with hydroxylamine hydrochloride in basic conditions. With this chemical system, SERS substrate preparation could be achieved at room temperature and in short time. The in situ prepared silver sol was used for trace analysis of several organic test molecules that were injected into the levitated SERS-active droplet again using the microdispenser. The concentration dependence of the SERS spectra was studied using 9-aminoacridine, revealing that down to the femtogram region high-quality SERS spectra could be obtained. Additionally, SERS spectra of 6-mercaptopurine, thiamine, and acridine were recorded in the levitated drop as well.     

A coupled boundary/finite element method for the computation of magnetically and electrostatically levitated droplet shapes

A coupled finite element and boundary element method is developed to predict the magnetic vector and scalar potential distributions in the droplets levitated in an alternating magnetic or electrostatic field. The computational algorithm entails the application of boundary elements in the region of free space and finite elements in the droplet region, the two being coupled along the droplet–air interface. The coupled boundary and finite element scheme is further integrated with a WRM‐based algorithm to predict the free surface deformation of magnetically and electrostatically levitated droplets. Several corner treatments for the boundary and finite element coupling and their implications to free surface calculations are discussed. Detailed formulation and numerical implementation are given. Numerical results are compared with available analytical solutions whenever available. A selection of computed results is presented for mag‐ netically or electrostatically levitated droplets under both terrestrial and microgravity conditions. Copyright © 1999 John Wiley & Sons, Ltd.     

Spin-up instability of a levitated molten drop in magnetohydrodynamic-flow transition to turbulence

We describe the spinning behavior of a suspended molten droplet subjected to electromagnetic heating. Our observations are derived from video images of droplets of palladium-silicon alloy in experiments on the MSL-1 (First Microgravity Science Laboratory) mission of the Space Shuttle (STS-83 and STS-94, April and July 1997). We inferred the resultant magnetohydrodynamic (MHD) flow inside the drop from motion of impurities on the surface. Digital particle tracking of the impurities is used to quantify the axial rotation of the levitated droplet. The analysis suggests that the levitated drop attains a constant rotational speed during the melting phase and formation of the co-rotating axisymmetric laminar toroidal structures. With continued electromagnetic heating, the sample's viscosity drops and the MHD flow accelerates, giving rise to instabilities of the internal flow. The rate of axial rotation increases significantly during this flow transition. The new data suggests a surprising interaction between the flow inside the levitated molten drop and the driving coils in the experiments. We explore the mechanisms that may be responsible for this spinning behavior.     

Melt Flow Instability and Turbulence in Electro-Magnetically Levitated Droplets

This article addresses fluid flow instabilities and flow transition to turbulent chaotic motions through numerical analysis and turbulence in electro-magnetically levitated droplets through direct numerical simulations. Numerical implementation and computed results are presented for flow instability and turbulence flows in magnetically levitated droplets under terrestrial and microgravity conditions. The linear melt flow stability is based on the solution of the Orr-Sommerfeld linearized equations with the base flows obtained numerically using high order numerical schemes. The resulting eigenvalue problems are solved using the linear transformation or Arnold's method. Melt flow instability in a free droplet is different from that bounded by solid walls and flow transits to an unstable motion at a smaller Reynolds number and at a higher wave number in a free droplet. Also, flow instability depends strongly on the base flow structure. Numerical experiments suggest that the transition to the unstable region becomes easier or occurs at a smaller Reynolds number when the flow structures change from two loops to four loops, both of which are found in typical levitation systems used for micro-gravity applications. Direct numerical simulations (DNS) are carried out for an electro-magnetically levitated droplet in a low to mild turbulence regime. The DNS results indicate that both turbulent kinetic energy and dissipations attain finite values along the free surface, which can be used to derive necessary boundary conditions for calculations employing engineering k--ε models.     

Normal Spectral Emissivity Measurement of Molten Cu–Co Alloy Using an Electromagnetic Levitator Superimposed with a Static Magnetic Field

The normal spectral emissivity of molten Cu–Co alloy with different compositions was measured in the wavelength range of 780 nm to 920 nm and in the temperature range of 1430 K to 1770 K including the undercooled condition by an electromagnetic levitator superimposed with a static magnetic field. The emissivity was determined as the ratio of the radiance from a levitated molten Cu–Co droplet measured by a spectrometer to the radiance from a blackbody calculated by Planck’s law at a given temperature, where a static magnetic field of 2.5 T to 4.5 T was applied to the levitated droplet to suppress the surface oscillation and translational motion of the sample. We found little temperature dependence of the normal spectral emissivity of molten Cu–Co alloy. Concerning the composition dependence, the emissivity decreased markedly above 80 at%Cu and reached that of pure Cu, although its dependence was low between 20 at%Cu and 80 at%Cu. In addition, this composition dependence of the emissivity of molten Cu–Co alloy can be explained well by the Drude free-electron model.     

Method to reduce errors of droplet sizing based on the ratio of fluorescent and scattered light intensities (laser-induced fluorescence/Mie technique)

The droplet sizing accuracy of the laser technique, based on the ratio of laser-induced fluorescence (LIF) and scattered light (Mie) intensities from droplets, is examined. We develop an analytical model of the ratio of fluorescent to scattered light intensities of droplets, which shows that the LIF/Mie technique is susceptible to sizing errors that depend on the mean droplet size and the spread of the droplet size distribution. The sizing uncertainty due to the oscillations of the scattered light intensity as a function of droplet size is first quantified. Then, a new data processing method is proposed that can improve the sizing uncertainty of the technique for the sprays that were examined in this study by more than 5% by accounting for the size spread of the measured droplets, while improvements of 25% are possible when accounting for the mean droplet size. The sizing accuracy of the technique is evaluated in terms of the refractive index of liquid, scattering angle, and dye concentration in the liquid. It is found that the proposed approach leads to sizing uncertainty of less than 14% when combined with light collection at forward scattering angles close to 60° and the lowest fluorescent dye concentration in the liquid for all refractive indices.     

Stability analysis of an acoustically levitated disk

In this paper, a model is developed for the stability analysis of an acoustically levitated disk on the basis of analyzing eddy acoustic streaming and acoustic viscous stress. In the model, the effect of the acoustic streaming outside the boundary layer that is on the surface of the levitated disk is properly taken into account. Also, the calculation of sound field and acoustic viscous stress is limited to the range that has a dominant effect on the stability. By this method, we obtain a quite accurate solution of the stability coefficient. For the small horizontal shift of a large levitated disk, the model is verified by the good agreement between the experimental and theoretical results. By means of this model and relevant experiments, some factors that affect the stability of the levitated disk are investigated, and useful guidelines for design and application are obtained. It is found that the range from the edge to the outermost nodal circle of the disk-shaped vibrator has a large effect on the stability of the levitated disk. To stabilize the levitated disk by acoustic viscous force, the distance between the edge and the outermost nodal circle of the vibrator must be larger than a critical value, which is determined by the driving frequency and the sound velocity of the fluid between the levitated disk and the vibrator. When this condition is satisfied, increasing the distance between the edge and the outermost nodal circle leads to a decrease in the stability. It is also found that the property of the fluid between the levitated disk and the vibrator has a large effect on the stability. It is easier to stabilize the levitated disk in steam than in air, but more difficult to do so in carbon dioxide and hydrogen. In addition, theoretical results show that increasing the weight per unit area of the levitated object increases the stability for a given vibrator velocity. The distribution of the acoustic viscous stress and the dependence of the stability coefficient and the holding force on the horizontal shift of the levitated disk, which are obtained by this study, also are useful to a better understanding of the stability of the levitated disk.     

Oscillation behavior of a high-temperature silicon droplet by the electromagnetic levitation technique superimposed with a static magnetic field

When a static magnetic field was applied to a Si droplet levitated by an electromagnetic force, only one peak was observed to remain in the frequency spectrum. It was the objective of this work to clarify whether this peak can be assigned to the m = ± 2 oscillation or to the rotation of the droplet. By analyzing the behavior of the deflection angle of the droplet in a top view, we conclude that this peak is not due to the surface oscillation of the droplet but to the droplet rotation.     

Fractionation of freshwater colloids and particles by SPLITT: analysis by electron microscopy and 3D excitation-emission matrix fluorescence

This paper reports the first application of a combined approach utilizing split-flow thin-cell (SPLITT) separation to size fractionate natural aquatic colloids and particles collected from freshwater samples. No sample preconcentration was performed although some samples were investigated after alteration of the ambient pH. The unfractionated and fractionated samples were analyzed by scanning electron microscopy (SEM), environmental SEM, and 3D excitation emission matrix fluorescence. Qualitative and quantitative results by microscopy indicated that SPLITT produces well-resolved fractionations at appropriate sizes but with some perturbation of the sample. In addition, tryptophan-like fluorescence was shown to be caused by different organic moieties compared with humic-like and fulvic-like fluorescence. Tryptophan-like fluorescence intensity is found mainly in the particulate material but is not pH dependent, while humic- and fulvic-like fluorescence intensities are dependent on pH but not on size. Fulvic-like fluorescence intensity normalized to absorbance, related to fluorescence efficiency and molar mass, varies with size.     

A novel flow-cytometry-based assay for cellular uptake studies of polyelectrolyte microcapsules

A flow-cytometry-based assay is presented with which the uptake of polyelectrolyte capsules can be quantified. The cavity of the capsules is loaded with the pH-sensitive dye SNARF, which emits in the red and green in alkaline and acidic environments, respectively. By recording the fluorescence intensities in the red and green channels, the localization of capsules associated with cells can be determined. Capsules adherent to the outer cell membrane fluoresce in the red due to the alkaline pH of the cell medium, whereas capsules internalized by cells fluoresce in the green due to the acidic pH in the endosomal/lysosomal/phagosomal compartments in which incorporated capsules are located. Adding the SNARF readout to the scattering signal typically derived with flow cytometry analysis allows for a more detailed quantitative analysis of particle uptake, which can also distinguish between adherent and ingested particles.