Effect of high pressure processing on the gel properties of salt-soluble meat protein containing CaCl2 and κ-carrageenan

The effects of high pressure processing (HPP) on the water-binding capacity and texture profile (TPA) of salt-soluble meat protein (SSMP) containing 0.2% CaCl₂ and 0.6% κ-carrageenan (SSMP-CK) gels were investigated. The results showed that 300–400 MPa improved water-binding capacity and decreased TPA parameters of SSMP-CK gels (P < 0.05), while 100 MPa could increase hardness and chewiness of the gels. The thermal transition temperature peak for the myosin head (T_peak1) of SSMP disappeared on addition of CaCl₂ and κ-carrageenan. 300 MPa produced a new peak, and caused a shift of the NH-stretching left peak and amide I and the disappearance of NH-stretching right peak. The destruction of network structure and the weakening of molecular interaction within the pressurized gels could result in the decrease of TPA parameters. Thus gelling properties could be modified by HPP, κ-carrageenan and Ca^2 +. It is of interest to develop low-fat and sodium-reduced meat products.     

Water holding capacity of sodium-reduced chicken breast myofibrillar protein gel as affected by combined CaCl2 and high-pressure processing

The water holding capacity (WHC) of sodium-reduced (0.3 m sodium chloride, corresponding to the salt percentage (w/v) of 1.755%) myofibrillar protein (MP) gel in response to combined calcium chloride (CaCl₂, 20, 60, 100 mm ) and high-pressure processing (HPP, 200 MPa, 10 min) was investigated. The results showed that 200 MPa + 20 mm CaCl₂ synergistically increased the WHC of MP gel via reducing particle size of MP solutions, strengthening hydrogen-bonding and disulphide-bonding, promoting formation of β-sheet and uncoiling of α-helix, exposing tryptophan residues, enhancing hydrophobic interactions of aliphatic residues and forming a compact and continuous networked gel structure. However, high concentrations (≥60 mm ) of CaCl₂ could attenuate the enhancing effects of HPP on the WHC by inducing decreased hydrogen bonds, fewer tryptophan residues exposed and coarser and aggregated gel structures with large cavities. Therefore, a combined moderate HPP and low concentration of CaCl₂ is a potential alternative for developing sodium-reduced meat products.     

Response surface methodology study on the optimisation of effects of fat,wheat bran and salt on chemical,textural and sensory properties of patties

A three-factor Box-Behnken design was adopted for studying the simultaneous effects of processing variables such as fat (10–30%), wheat bran (5–15%) and NaCl (0–2%) on physicochemical, textural and sensory properties of cooked beef patties. In addition, the ridge analysis was conducted to find the values of processing variables that maximise and minimise the texture profile analysis (TPA) parameters (hardness, chewiness, gumminess, adhesiveness, springiness, resilience and cohesiveness) and sensory ratings. Experimental design allowed for evaluation of potential interactive and quadratic effects between these variables; fat wheat bran and NaCl. It was found that protein and fat contents of patties was decreased and increased, respectively as the amount of wheat bran increased. NaCl increased the ash content of patties. Fat and wheat bran had a notable influence on textural properties, which improved the textural properties at certain levels. Lower addition of wheat bran and NaCl into patty resulted in higher acceptable product with respect to firmness, juiciness and overall quality properties.     

Tenderness and flavor of leg cuts from meat goats influenced by calcium chloride injection

This study was conducted to assess the potential of improving tenderness of chevon using calcium chloride (CaCl₂) injection and its effect on the palatability characteristics of chevon. Primal leg cuts from meat goats were allotted to one of four treatments: either no injection (control) or injection with water, CaCl₂ (food grade, 2.2% w/v), or CaCl₂ plus a spice mix. The CaCl₂ injection improved tenderness of goat leg cuts, proven by Warner–Bratzler shear force values and sensory panels. Furthermore, panelists were not able to detect off-flavor problems associated with CaCl₂ injection. When CaCl₂ was injected into goat leg cuts with the beef spice mixture, it resulted in a more desirable flavor. Calcium injection did not influence flavor volatile compounds in cooked chevon leg cuts. The results indicate that CaCl₂ plus spice mix injection can be applied to improve tenderness of goat meat without detrimental effects on other sensory characteristics.     

Effects of Sodium Chloride Concentration on Firmness Retention of Cucumbers Fermented and Stored with Calcium Chloride

The firmness of cucumbers brined at 0.2% CaCl₂ was retained during fermentation (1 month) and storage (12 months) when 2.6, 4.2 or 5.8% NaCl also was present. Firmness retention was not improved (P≥0.05) during storage by increase in NaCl concentration to 11.9% after fermentation. In the absence of added NaCI, cucumbers were firm after fermentation, but firmness was reduced during storage to 69% of initial for whole cucumbers, 64% for mesocarp tissue, and 32% for endocarp tissue. Addition of uncharacterized softening enzymes. extracted from debris collected at a cucumber grading operation, resulted in softening of cucumbers brined at 1.8% NaCl in the absence of added CaC1₂. Addition of CaCl₂ reduced but did not eliminate softening by ths extract. Results indicated that the firmness of brined cucumbers could be retained at appreciably lower NaCl concentrations than those traditionally used but that the lower limit of NaCl required to prevent softening by possible contaminating enzymes is yet to be established.     

Response surface methodology study on the optimisation of effects of fat, wheat bran and salt on chemical, textural and sensory properties of patties

A three-factor Box-Behnken design was adopted for studying the simultaneous effects of processing variables such as fat (10–30%), wheat bran (5–15%) and NaCl (0–2%) on physicochemical, textural and sensory properties of cooked beef patties. In addition, the ridge analysis was conducted to find the values of processing variables that maximise and minimise the texture profile analysis (TPA) parameters (hardness, chewiness, gumminess, adhesiveness, springiness, resilience and cohesiveness) and sensory ratings. Experimental design allowed for evaluation of potential interactive and quadratic effects between these variables; fat wheat bran and NaCl. It was found that protein and fat contents of patties was decreased and increased, respectively as the amount of wheat bran increased. NaCl increased the ash content of patties. Fat and wheat bran had a notable influence on textural properties, which improved the textural properties at certain levels. Lower addition of wheat bran and NaCl into patty resulted in higher acceptable product with respect to firmness, juiciness and overall quality properties.     

Effect of high-pressure treatment on the quality of prepared chicken breast

In this study, the effect of high-pressure processing (HPP) on the physico-chemical properties and microstructure of prepared chicken breast meat products was investigated. The result showed that the pH and TBARS values of the prepared chicken breast gradually increased, while the total volatile basic nitrogen value constantly decreased with the increase of pressure (0.1–500 MPa). In addition, the colour attributes (L* and a* values) and texture indexes (hardness and chewiness) significantly improved as the pressure increased. The results of low-field nuclear magnetic resonance demonstrated that the peak area of immobilised water (T₂₂) first enhanced and then declined with the increase of pressure, reaching the maximum value at 200 MPa. Moreover, the integrity of microstructure and protein secondary structure of prepared chicken breast were also disrupted. These findings could serve as a theoretical basis for a better application of HPP in prepared chicken breast meat products.     

Gel properties,physicochemical properties,and sensory attributes of white leg shrimp (Litopenaeus vannamei) surimi gel treated with sodium chloride (NaCl) substitutes

This study investigated the effects of different NaCl substitutes on the gel properties, physicochemical properties, and sensory attributes of shrimp surimi gel to produce high-quality reduced-salt shrimp surimi gel. The results showed that CaCl₂, calcium ascorbate (Vc-Ca), and L-arginine (L-Arg) could significantly improve the gel strength and texture of shrimp surimi gel compared to NaCl. The addition of CaCl₂, Vc-Ca, and L-Arg significantly increased the number of disulphide bonds and the content of β-sheet structures compared to NaCl. The electrophoretic analysis revealed that CaCl₂, Vc-Ca, and L-Arg had protective effects on the myosin heavy chain during thermal gelation. Additionally, CaCl₂ and L-Arg promoted the cross-linking of myofibrillar proteins to form the denser and less porous microstructures, and thus improved the gel properties of shrimp surimi gel. In general, the introduction of L-Arg as a substitute for NaCl acquired the best gel properties and sensory attributes of shrimp surimi gels, followed by CaCl₂ and Vc-Ca.     

Effects of high pressure level and holding time on properties of duck muscle gels containing 1% curdlan

The effect of high pressure processing (HPP) on properties of duck muscle gels (DMG) containing 1% curdlan was investigated. The application of > 300 MPa could result in the decrease of cooking loss of DMG in water binding capacity, the increase of L value and the decrease of a value and b value in color, the increase of hardness, springiness, cohesiveness and chewiness in textural parameters (P < 0.05), while the pressure-holding time had no obvious influence. Those experimental results could be attributed to the interactions among protein molecules and the interactions between protein molecules and curdlan molecules created or enhanced by HPP. Overall, the use of curdlan instead of fat and the application of HPP may provide a novel approach to achieve low-fat (< 6% fat) and low-salt (1% salt) DMG products with good properties and high yields.

Industrial relevance

To provide healthier meat foods like low-fat (< 6% fat) and low-salt (about 1% salt) duck meat products, the application of HPP might be of great interest for industrial manufacture and can yield the products bearing high water binding capacity and good textural properties.     

PECTIC SUBSTANCES AND FIRMNESS OF CUCUMBER PICKLES AS INFLUENCED BY CACL2, NACL AND BRINE STORAGE1

The effects of brine treatments (CaCl₂ and NaCl) and storage on pectic substances and texture of cucumber pickles were examined. Firmness of cucumber pickles was closely associated with the solubility characteristics and degree of esterification (DE) of pectic substances. Brining, storage, time of CaCl₂ addition and concentration of NaCl and CaCl₂ were all observed to influence the characteristics of pectic substances. Important to preventing softening was maintenance of pectic substances not extractable (NXP) by conventional methods, i.e., by water (WSP), the chelator sodium hex-ametaphosphate (CSP), and dilute alkali (OHSP). Erosion of NXP consistently resulted in increased levels of CSP and reduced firmness. Reducing the amount of demethylation of pectins was also associated with maintaining firmness. Although the DE declined rapidly during brining, tissues from treatments that enhanced firmness had pectic substances with the highest DE. CaCl₂ added to brine at the beginning of fermentation was most effective in preventing the demethylation of pectins and solubilization of NXP. In contrast, delayed addition of CaCl₂ and storage in low NaCl concentration (5% or less) caused greater pectin demethylation, erosion of NXP with concomitant increases in CSP and tissue softening.     

High pressure processing of meat batters with added walnuts

Various model systems were designed in order to analyse the way in which addition of different levels of walnut (0, 10, 20%) and processing by high pressure (HPP) (400 MPa for 10 min at 10 °C) influenced the physico‐chemical properties of cooked (70 °C for 30 min) meat batters. The addition of walnut increased the fat level and decreased the moisture content in the meat batters. All of the meat batters exhibited good water and fat binding properties. The hardness, cohesiveness, springiness and chewiness of cooked products were reduced by addition of walnut but were unaffected by HPP. Incorporation of nuts in meat products can potentially be used to confer cardiac health benefits.     

The effect of chloride salts onYersinia enterocoliticain meat

The populations ofYersinia enterocolitica(serotypes 0:3 and 0:8) exposed to NaCl, KCl and CaCl₂in meat, decreased as the concentration of each salt increased. Calcium chloride was significantly (α=0.05) more effective against serotype 0:8. Sodium nitrite was synergistic to the anti-yersinial effect of NaCl. Mathematical equations were developed for the prediction of the population ofY.enterocoliticain meat, exposed to the chloride salts. In addition, these equations can also be used to determine the concentration of a chloride salt required for attaining a predetermined level of the pathogen. The Anti-yersinial Index (AI) demonstrated that CaCl₂was most efficient against serotype 0:3 while the higher concentrations of KCl (1.8 and 2.2% w/w Cl) were most efficient against serotype 0:8.     

猪肉丸主要原料对其凝胶性质和感官品质的影响

研究主要原料玉米淀粉、大豆分离蛋白、肥肉、水,在不同添加量时,对猪肉丸凝胶性质中硬度、弹性、黏聚性、咀爵性、蒸煮损失率和感官品质的影响.通过质构分析和感官评价,结果表明:玉米淀粉的不同添加量对猪肉丸的硬度、弹性、黏聚性、咀嚼性、蒸煮损失率有显著差异,在添加量22％时感官评分最高;大豆分离蛋白的不同添加量对猪肉丸的硬度、弹性、咀嚼性、蒸煮损失率有显著差异,在添加量为2.0％时感官评分最高;肥肉的不同添加量对猪肉丸的硬度、咀嚼性、蒸煮损失率有显著差异,在添加量30％时感官评分最高;在水的不同添加量对猪肉丸硬度、黏聚性、咀嚼性、蒸煮损失率有显著差异,在添加量35％时感官评分最高.     

NaCl对猪肉干燥过程中质构特性的影响

以猪背最长肌为研究对象,经不同浓度NaCl溶液腌制后,60 ℃下热风干燥后其质构特性参数随含水率和NaCl含量的变化规律。结果表明:随含水率降低,各样品的剪切力值、硬度及咀嚼性均逐渐增加,而弹性、内聚性则逐渐下降;在相同含水率下,腌制液NaCl浓度≤8%腌制样的剪切力值、硬度、咀嚼性随NaCl浓度的增大而增大,腌制液NaCl浓度＞8%腌制样的上述参数随NaCl浓度增大而减小,各腌制样的弹性随腌制液NaCl浓度升高而逐渐减小,内聚性受NaCl浓度的影响较小。由部分物理指标与质构特性参数之间的相关性分析可知:肉样的质构特性参数与样品的含水率、横向收缩比(LSR)、纵向收缩比(TSR)、体积变化率等极显著相关(p<0.01),而相关系数的大小与腌制液NaCl浓度有关。研究结果为腌制肉的品质控制提供参考。     

Calcium Chloride and Potassium Sorbate Reduce Sodium Chloride used during Natural Cucumber Fermentation and Storage

Cucumber fermentation characteristics and pickle quality were evaluated in brines containing equilibrium concentrations of 0-0.4% CaCl₂, 0-0.4% potassium sorbate and 0-10% NaCl. Changes in brine pH and acidity, cucumber texture and color, coliforms, lactic acid and total bacteria, yeasts and molds were followed over time. Results indicated that cucumber spoilage would eventually take place if NaCl or potassium sorbate were not present in the brine. The presence of CaCl₂ helped maintain cucumber firmness. A synergistic action between NaCl, CaCl₂ and potassium sorbate was seen, which allowed good quality pickles to be produced when moderate amounts of all three components were present in the brine (5% NaCl, 0.2% CaCl₂, 0.2% potassium sorbate).     

微波加热及NaCl添加量对牦牛肉糜凝胶特性和保水性的影响

微波加热是食品加工的一种新型技术,为探讨微波加热对牦肉糜凝胶特性的影响,采用单因素试验设计,研究微波加热的时间、功率以及NaCl添加量对牦牛肉糜凝胶质构特性和保水率的影响。以水浴为对照组,利用质构仪测定牦牛肉糜凝胶硬度、咀嚼性、弹性和回复性。结果表明：随着对牦牛肉糜微波加热时间的延长,其凝胶硬度、咀嚼性、弹性逐渐下降（P＜0.05）,回复性逐渐增加（P＜0.05）,保水率显著下降（P＜0.05）;而随着微波功率增大,牦牛肉糜凝胶的硬度、咀嚼性降低（P＜0.05）,弹性变化不大（P＞0.05）,蒸煮损失率明显增加（P＜0.05）;同时,随着NaCl添加量增加,牦牛肉糜凝胶保水率显著增加（P＜0.05）。本研究为微波技术在肉制品加工中应用提供了理论依据。     

The influence of additives on properties of heat‐induced gels from salt‐soluble proteins extracted from goose

Calcium chloride (CaCl₂) and phosphates are important additives to improve product quality during meat processing. Response surface methodology was used to study the influence of CaCl₂ and phosphates on the hardness, water‐holding capacity (WHC) and ultra‐structure of salt‐soluble goose meat protein gels. The results show that the hardness and WHC of salt‐soluble protein gels increased significantly when CaCl₂ concentration was increased and phosphates were added. Scanning electron microscopy showed that tetrasodium pyrophosphate (TSPP) and sodium tripolyphosphate (STPP) had a greater impact on the cross‐linking and pore diameter of the gel networks than sodium hexametaphosphate (SHMP). At the 0.02 m and 4:3:2 of CaCl₂ concentration and the ratio of TSPP, SHMP and STPP, hardness and WHC values were 114.55 gf and 96.65%, which corresponded to the prediction value of our model. Further results showed that the hardness and WHC of gels reached the maximum with 0.3% of phosphates levels.     

High-Pressure Pretreatment to Improve the Water Retention of Sodium-Reduced Frozen Chicken Breast Gels with Two Organic Anion Types of Potassium Salts

The effect of high-pressure pretreatment (HPP, 100–300 MPa) before heating on the water holding capacity (WHC) and cooking loss (CL) of sodium-reduced (1.35% NaCl) frozen chicken breast gels with the organic anion types potassium lactate (KL-gel) and potassium citrate (KC-gel) was investigated. The results showed that 100–300 MPa could significantly decrease the CL of both gels, and 200 MPa increased the WHC of the KC-gel (P?<?0.05). HPP could decrease the storage modulus (G′) of meat batters, increase the thermal stability of their proteins, facilitate gelation with a dense and uniform microstructure, and immobilize free water, thus improving the gel’s water retention. The water retention of the pressurized sodium-reduced gel varied with the organic anion types of salts. The water retention was better with pressurized KC-gel than with KL-gel, resulting from higher pH and thermal stability of the batters. These results indicate that proper HPP could improve the water retention of sodium-reduced meat gels with organic potassium salts, thus increasing the economic benefit and properties of sodium-reduced products from frozen meat.     

Effect of high pressure on moisture and NaCl diffusion into turkey breast

High-pressure processing (HPP) affects food component diffusion. This study evaluated pressure (0.1-300 MPa) effect on the diffusion of NaCl and water into turkey breast at 25 °C. NaCl and water diffusion were found to be a function of pressure and holding time. During the pressure come-up-time, diffusion of water and NaCl into the sample was enhanced as compared to control. The diffusion coefficient of moisture infusing out of the sample was found to be a minimum at 150 MPa. The diffusion coefficient of NaCl infusing into the sample was maximum at 150 MPa. Within the range of experimental conditions studied, 150 MPa treatment (up to 15 min holding time) yielded meat samples with minimum hardness, gumminess, and chewiness. Ultrastructure images revealed that 150 MPa treatments caused swelling of myofibrils, disappearance of the M-line, reduced difference in the density of the A-band and I-band and breaking of segments of Z-line. HPP could be a useful technique for the salting of turkey meat.     

Application of emerging non-thermal technologies to sodium reduction in ready-to-eat fish products

This study investigated the isolated and combined effect of UV-C (0.310 J/cm²) and high hydrostatic pressure (HHP; 300 MPa for 5 min at 25 °C) on the quality parameters of cooked fish batter with reduced salt content by 25% and 50%. The treatments did not affect sodium chloride concentration, redness, yellowness, cohesiveness, springiness, and resilience (p > 0.05). CON (100% sodium chloride - NaCl), UV25%, and HHP25% had similar cooking loss (CL), lightness, hardness, chewiness, and salty taste (p > 0.05). UV + HHP25% showed higher CL and lower salty taste compared to CON, UV25%, and HHP25% (p < 0.05). UV50% and HHP50% had higher CL, lightness, hardness and chewiness, and lower salty taste than CON, UV25%, and HHP25% (p < 0.05). UV + HHP50% showed the highest CL, hardness and chewiness, while the lowest salty taste and higher lightness than CON (p < 0.05). Therefore, UV-C at 0.310 J/cm² or HHP at 300 MPa for 5 min is a potential alternative to developing ready-to-eat fish products reduced by 25% NaCl.