Thin-Layer Drying Characteristics and Quality Evaluation of Air-Dried Ganoderma Tsugae Murrill

Ganoderma is normally dried to extend its shelf life without using chemical preservative and to concentrate the medicinal value in the fruiting body. Convective hot air drying characteristics of Ganoderma tsugae Murrill were evaluated in hot air circulated oven at different drying temperatures, sizes, and air flow rates. The drying kinetics of Ganoderma tsugae in kidney shape and slices were investigated and compared at different drying conditions. The variation of effective moisture diffusivity values at decreasing moisture contents during drying was determined from the drying data. Four well-known thin-layer drying models were fitted to the experimental data and the Midilli model was found to satisfactory describe the drying characteristics of kidney-shaped Ganoderma tsugae. Ganoderma tsugae dried at 50°C with air velocity of 1.401 ms^?1 showed the highest retention of crude ganoderic acid content compared to other drying conditions.     

Dehydration of Concentrated Ganoderma lucidum Extraction by Combined Microwave-Vacuum and Conventional Vacuum Drying

Combination of microwave-vacuum drying and conventional vacuum drying was investigated as a potential method for drying concentrated Ganoderma lucidum extraction. The Ganoderma lucidum was extracted by hot water (60–65°C) and then concentrated to moisture of about 70% (wet basis) in a rising-film evaporator. The concentrated sample was dried by microwave-vacuum until the moisture content reached 10% (wet basis), and then by conventional vacuum drying at the temperature of 55–60°C to final moisture content about 6% (wet basis). The retention of polysaccharide and triterpenes of Ganoderma lucidum dried by this method were evaluated and compared with those dried by freeze drying and conventional vacuum drying alone. The comparison showed that the quality of extraction dried by the current method was close to that of freeze-dried extraction and much better than that of conventional vacuum-dried ones.     

Convective Drying of Ganoderma tsugae Murrill and Effect of Temperature on Basidiospores

In this study, drying of Ganoderma tsugae Murrill slices was carried out at different temperatures in a hot air circulating oven. The objectives were to investigate the drying characteristic and drying kinetics of the Ganoderma slices under constant air velocity and slice thickness. In addition, the effect of drying temperatures on the size and surface structure of Ganoderma tsugae basidiospores were studied. Using statistical analysis, a two-term model was found to fit best with the experimental data. Convective drying of Ganoderma tsugae slices at 60°C shows the minimum heat deformation to the size and surface structure of basidiospores.     

Combined ultrasound and infrared assisted conductive hydro-drying of apple slices

Abstract

Conductive hydro-drying also known as Refractance Window drying is a relatively new drying technology, which uses hot water to carry thermal energy to materials to be dehydrated. It has a high retention of heat sensitive quality parameters (vitamins, antioxidants, and color) with better energy efficiency than freeze-drying as well as many other conventional drying methods. A new ultrasound and infrared assisted conductive hydro-dryer (UIACHD) was developed to increase drying rate while reducing required hot water temperature and increasing the drying material thickness. The goal of this study was to evaluate the performance of the new dryer and to compare the performance of a pilot scale continuous UIACHD with a freeze-dryer and a cabinet dryer in drying apple slices. The physiochemical characteristics of the dried apple slices including flavonoid content, total phenolic compounds, antioxidant activity, vitamin C content and color were measured. In addition, the energy consumption and energy efficiency of the dying methods were evaluated. Results showed that combining ultrasound and infrared with conductive hydro-drying can result in higher drying rates and lower product moisture content. Quality of UIACHD dried apple slices was close to the freeze-dried products and it was significantly better than the cabinet dried products. Moreover, the energy efficiency of UIACHD was considerably better than the cabinet dryer and the freeze-dryer. The results of this study showed that combining ultrasound and infrared with conductive hydro-drying can lead to an energy-efficient process with good quality retention ability.     

Glycosylation of Ganoderic Acid G by Bacillus Glycosyltransferases

Ganoderma lucidum is a medicinal fungus abundant in triterpenoids, its primary bioactive components. Although numerous Ganoderma triterpenoids have already been identified, rare Ganoderma triterpenoid saponins were recently discovered. To create novel Ganoderma saponins, ganoderic acid G (GAG) was selected for biotransformation using four Bacillus glycosyltransferases (GTs) including BtGT_16345 from the Bacillus thuringiensis GA A07 strain and three GTs (BsGT110, BsUGT398, and BsUGT489) from the Bacillus subtilis ATCC 6633 strain. The results showed that BsUGT489 catalyzed the glycosylation of GAG to GAG-3-o-β-glucoside, while BsGT110 catalyzed the glycosylation of GAG to GAG-26-o-β-glucoside, which showed 54-fold and 97-fold greater aqueous solubility than that of GAG, respectively. To our knowledge, these two GAG saponins are new compounds. The glycosylation specificity of the four Bacillus GTs highlights the possibility of novel Ganoderma triterpenoid saponin production in the future.     

Formulation and Cost-Effective Drying of Probiotic Yeast

Saccharomyces boulardii yeast is considered as a probiotic according to the World Health Organization (WHO). Like any other probiotic, Saccharomyces boulardii is available as a freeze-dried formulation. Although freeze drying is the most preferred method of preserving the microorganisms, the process is very expensive. The cost of capsules containing freeze-dried probiotic yeast is certainly out of reach of the underprivileged population of the world. Hence, the present work focuses on developing a new formulation and cost-effective drying process for probiotic yeast. Freeze drying was compared with fluidized bed drying (FBD) and heat pump–assisted fluidized bed drying (HP-FBD) techniques for drying of Saccharomyces boulardii formulation. The process involves addition of lactose and microcrystalline cellulose to the biomass obtained by centrifugation and then making the dough. This blend is then extruded and spheronized to form granules. Heat pump fluidized bed drying of the formulation resulted in higher activity retention compared to atmospheric fluid bed drying. This encouraged further experimentation using HP-FBD to study the effect of various operating variables such as air temperature, air humidity, and velocity on the drying kinetics and the deactivation kinetics. It was observed that the temperature and humidity play a major role in the deactivation and drying, whereas the change in air velocity has comparatively less effect on both parameters. The effective diffusivity and the deactivation rate constants were correlated to the temperature using an Arrhenius-type equation.     

Combined Microwave-Vacuum and Freeze Drying of Carrot and Apple Chips

A combination of microwave-vacuum (MWV) drying and freeze drying was investigated as potential means for drying carrot and apple chips. The sample was first dried by microwave-vacuum to dehydrate some amount of internal free water and then by freeze drying to a final moisture content of less than 7% (wet basis). Chemical properties (carotene and vitamin C retention) and physical properties (shrinkage, color, texture, and rehydration ratio) of carrot and apple slices dried by this method were evaluated and compared with those dried by freeze drying alone, MWV drying alone, and conventional hot air drying, respectively. The comparison showed that the carotene retention of carrot slices and the vitamin C retention of apple slices dried by the current method were close to those of freeze-dried carrot and apple slices and much better than those of conventional hot air–dried ones. The samples prepared by the current method exhibited very close rehydration capacity, color retention, and texture with those of the freeze-dried ones but with a little higher shrinkage. However, the samples still showed the attractive external appearance without marked warp.     

Intensification of Freeze-Drying Rate of Bacillus subtilis MTCC 2396 Using Tungsten Halogen Radiation: Optimization of Moisture Content and α-Amylase Activity

A novel freeze-drying protocol has been explored to render fast and cost-effective freeze drying of hyperamylase producing Bacillus subtilis MTCC2396 employing a tungsten halogen lamp radiator (THLR) as a heat source. Response surface methodology assessed the maximum reduction in moisture content (96.07%) and minimum reduction in α-amylase (EC 3.2.1.1) activity (1.02%) in 4 h drying time at 42.5°C radiation temperature. α-amylase activity (0.046 U) and final moisture content (3.93%) of the optimally freeze-dried bacterial strain appeared satisfactory. The freeze-drying time using THLR (4 h) is remarkably lower compared to that under a conventional conductive plate heater (CPH) (10 h) at otherwise identical conditions. The higher effective moisture diffusivity of 0.0052 to 0.0078 m ²/s under THLR compared to 0.00084 to 0.0015 m ²/s under CPH (corresponding to 20–50°C) advocated the superiority of the THLR heating protocol. The higher efficacy of THLR was also evidenced through lower activation energy (8.42 kJ/mol) of moisture diffusion compared to that (12.051 kJ/mol) of CPH. The optimally freeze-dried bacteria demonstrated the same growth rate in addition to exhibiting excellent retention of bioremedial (Hg²⁺ removal) activity to that of the control.     

Effect of two-stage dehydration on retention of characteristic flavor components of flue-cured tobacco in rotary dryer

Two-stage dehydration method of flue-cured tobacco in rotary dryer was experimentally evaluated and compared to the single-stage dehydration process. The characteristic flavor components in dried cut tobacco, including major Maillard reaction compounds and carotenoid degradation products, as well as drying rate were analyzed for different two-stage dehydration operations. The results showed that both the pre-drying temperature and intermediate moisture content had a significant effect on the overall drying rate of cut tobacco during two-stage drying. On the other hand, the retention of characteristic flavor components in tobacco, especially Maillard reaction compounds retention, was influenced significantly by the final drying temperature as well as intermediate moisture content. The 10°C increase in the final drying temperature from 100 to 110°C reduced the retentions of Maillard reaction compounds and carotenoid degradation products by 14.5 and 9.4%, respectively. Change of intermediate moisture contents from 19 to 15% reduced the Maillard reaction compounds retention by 14.7%. Given the consideration of characteristic flavor components’ retention and drying process efficiency, two-stage dehydration of cut tobacco in rotary dryer could reach a better performance as compared with the single-stage dehydration. The cylinder-wall temperature combination of 140/100°C with the 17% intermediate moisture content could be used as the optimal two-stage condition for the investigated flue-cured tobacco.     

Effects of Acetic Acid Pretreatment and Hot Air Drying on Resistance of Salmonella on Cabbage Slices

The heat resistance of Salmonella anatum inoculated onto the surface of cabbage slices as affected by acetic acid pretreatment (0.5–1.5% v/v) and hot air drying at 50–60°C was investigated. Approximately 1.5 log₁₀ of Salmonella numbers was reduced after soaking the vegetables in acetic acid solution. The inhibitory effect of acetic acid on the bacteria was more pronounced during drying. The heat resistance of Salmonella on the cabbage surface treated with acetic acid decreased considerably compared with untreated cabbage. Moreover, the vegetable treated with higher acetic acid concentration resulted in a higher degree of acid injury and hence increased the susceptibility to heat during drying. The results revealed that acetic acid pretreatment may be used as an additional processing step to increase the safety margin of dried vegetable products.     

Two-stage extraction of antitumor,antioxidant and antiacetylcholinesterase compounds from Ganoderma lucidum fruiting body

Ganoderma lucidum has been used in oriental medicine for its contribution to vitality and longevity. None of them report about targeted antitumor activity against adenocarcinoma cells, or antioxidant and antiacetylcholinesterase activity for potential application in treatment of Alzheimer's and other neurodegenerative diseases. To date, there are a few studies available concerning supercritical carbon dioxide extraction of biologically active compounds from G. lucidum fruiting body. In our study, two stage extractions of biologically active compounds from G. lucidum were performed. First, supercritical carbon dioxide was used as extraction solvent. Next, the same material was used for hot water isolation of biologically active polysaccharides. Cytotoxicity, antioxidant and antiacetylcholinesterase activity were tested for all obtained extracts. Additionally, the effect of extraction process conditions on the biological activity of extracts was assessed.     

Formaldehyde as a Chemical Defence Agent of Fruiting Bodies of Mycena rosea and its Role in the Generation of the Alkaloid Mycenarubin C

Mycenarubin C, a previously unknown red pyrroloquinoline alkaloid, was isolated from fruiting bodies of the mushroom Mycena rosea and its structure was elucidated mainly by NMR spectroscopy and mass spectrometry. Unlike mycenarubin A, the major pyrroloquinoline alkaloid in fruiting bodies of M. rosea, mycenarubin C, contains an eight-membered ring with an additional C₁ unit that is hitherto unprecedented for pyrroloquinoline alkaloids known in nature. Incubation of mycenarubin A with an excess of formaldehyde revealed that mycenarubin C was generated nearly quantitatively from mycenarubin A. An investigation into the formaldehyde content of fresh fruiting bodies of M. rosea showed the presence of considerable amounts of formaldehyde, with values of 5 μg per gram of fresh weight in fresh fruiting bodies. Although mycenarubin C did not show bioactivity against selected bacteria and fungi, formaldehyde inhibits the growth of the mycoparasite Spinellus fusiger at concentrations present in fruiting bodies of M. rosea. Therefore, formaldehyde might play an ecological role in the chemical defence of M. rosea against S. fusiger. In turn, S. fusiger produces gallic acid—presumably to detoxify formaldehyde by reaction of this aldehyde with amino acids and gallic acid to Mannich adducts.     

Studies on Decreasing Energy Consumption for a Freeze-Drying Process of Apple Slices

The shape, color, flavor, and rehydration capacity of freeze-dried (FD) products are all better than other dried products. However, the energy consumption during FD is very high, which limits the application of this drying method for common materials. In this article, microwave vacuum drying (MWVD) was applied before or after FD to decrease the energy consumption during FD. Moreover, energy consumption was divided into two parts: valid and invalid energy consumption. Apple slices were used as an example to calculate the saving percentage of invalid energy consumption by comparing combination drying with FD. Apple slices freeze dried for 8.28 h first and then dried by MWVD have the best appearance, with a savings of 39.20% in invalid energy consumption. But apple slices freeze dried for 6 h first followed by MWVD have the highest savings of invalid energy consumption, 54.02%, while still maintaining an acceptable appearance.     

Physical and Functional Properties of Blackberry Freeze- and Spray-Dried Powders

The aim of the present work was to develop two products from blackberry juice by freeze and spray drying with potential use as food colorants or healthy ingredients. A characterization of the physical and functional properties of the powdered juices was done. Maltodextrin or a mixture of trehalose and maltodextrin were assessed as carrier matrices. Freeze-dried, maltodextrin-containing powders presented the best retention of bioactive compounds and antiradical activity; however, they showed a narrow relative humidity range for storage in the glassy state. Spray-dried powders showed better physical properties, bearing higher glass transition temperature and lower molecular mobility than freeze-dried formulations.     

Analysis of CO2 Transcritical Cycle Heat Pump Dryers

The heat pump dryer with CO₂ as the working medium is investigated in this study. The drying systems were designed based on the thermophysical properties and characteristics of CO₂ cycle. The two-stage drying of CO₂ transcritical cycle heat pumps are presented and discussed in comparison with the single compression cycle single-stage heat pump dryer, the two-stage drying heat pumps, and electric drying system. The analysis indicates that the specific moisture extraction ratio of the two-stage drying system can be higher than that of the single drying CO₂ system. The efficiency of energy utilization of the CO₂ heat pump dryer was higher than that of an electric dryer.     

THE EFFECT OF DRYING AND STORAGE OF SOYBEAN ON THE QUALITY OF BEAN,OIL, AND LECITHIN PRODUCTION

ABSTRACT

A comparative study of soybean drying was carried out between one-stage and two-stage (high–low temperature) drying with shade drying as control. In one-stage drying, the beans were dried at 80, 100, 120, and 140°C, respectively while in two-stage drying, beans were dried at 100, 120, and 140°C in the first stage followed by 80°C in the second stage. The evaluation values after drying showed that high drying temperature caused high skin cracks of the beans. Qualities of extracted crude oil characterized by free fatty acids (FFA), peroxide value (PV), phospholipids (PL) and nonhydratable phospholipids (NHP) were not significantly effected at p<0.05 by different drying methods but NHP had the increasing trend with the drying temperature and two-stage dried beans tended to have NHP higher than one-stage dried beans. The quality of protein in meal determined in terms of protein dispersibility index (PDI) was less than 15% when drying temperature in one-stage drying was higher than 80°C and when the temperature at the first stage of two-stage drying was higher than 100°C. Drying temperature also had the effect on the quality of lecithin when considered in term of the acetone insoluble value (AI). Free fatty acids of extracted crude oil increased when dried beans were stored for two months at 35°C while PL decreased especially in the first month of storage. However, the AI value of lecithin did not change with time.     

Moisture Sorption Isotherm Characteristics of Freeze-Dried D-Limonene Emulsions in Modified Chitosan and Maltodextrin

This article reports on modified chitosan as an alternative substance for protecting loss of volatile compounds during freeze drying. Moisture sorption isotherms of freeze-dried D-limonene emulsions in modified chitosan were determined at 15, 25, and 35°C. The data were adjusted to the GAB model. Maltodextrin was used in a parallel experiment. Flavor released from microcapsules was measured. The monolayer humidity, the sorption heat, the diffusivity coefficients, and the surface area of freeze-dried D-limonene emulsions were determined.     

Comparative evaluation of physical properties and aroma profile of carrot slices subjected to hot air and freeze drying

In the present study, carrots were subjected to hot air- and freeze-drying processes and their physical parameters and aromatic profile were compared. Water activity, shrinkage, hardness, springiness, cohesiveness, chewiness, rehydration ratio, and color measurements show that the convective drying of carrot slices lead to major changes in physical and aroma characteristics. The volatiles from air-drying of carrots contain terpenes, aldehydes, and alcohols which contribute to their aroma. The terpene, patchoulene imparting a spicy odor is reported for the first time in fresh carrots and it gets completely degraded during air dehydration. The key flavor components of fresh carrots were mostly retained during drying. The analytical results confirm that freeze-dried products had higher aroma retention.     

Moisture flow characteristics and variation patterns of dielectric constants in bitter gourd slices during microwave hot-airflow vibrating drying

Abstract

The uniformity of microwave-dried bitter gourd slices was poor, and a microwave hot-airflow vibrating drying (MHAVD) apparatus was used to carry out an in-depth elucidation of moisture flow characteristics underlying the variation in dielectric characteristics. Low-field nuclear magnetic resonance and imaging (LF-NMR/MRI) was used to measure the moisture state and distribution during different drying stages, and the relationship model between moisture semaphore and moisture ratio were analyzed. A vector network analyzer was used to measure the patterns in the variation of dielectric characteristics in bitter gourd slices at different drying stages. As a result, the temperature and moisture distribution of bitter gourd slices during MHAVD was uniform caused by hot air and vibration, and the water semaphore in NMR gradually decreased, but the bound water semaphore increased during certain drying stages. There is a significant linear relationship between the total semaphore A₂ and the moisture ratio M_R in bitter gourd slices per unit dry basis mass in the NMR spectrum (R² = 0.99). As moisture decreases, the ability of the material to store microwaves and convert electromagnetic energy to heat energy decreases, the ability of the material to absorb microwaves decreases, and the microwave penetration significantly increases. The moisture flow characteristics in bitter gourd slices is related to the variation of dielectric characteristics during MHAVD, and drying uniformity was improved.     

Influence of heat pump drying on tomato flavor

ABSTRACT

Tomato flavor from a heat pump dehumidifier dryer was characterized and compared to fresh and freeze-dried tomato samples for the retention of fresh flavor compounds. The monitored quality parameters were nonvolatile, volatile, and odor intensity. There were no differences in nonvolatile profiles; however, significant changes in key odor-impact volatiles were observed. Commercial spray-dried tomato was also evaluated to examine thermally induced volatile changes. The volatile and sensory profiles of heat pump dried tomato were comparable to freeze-dried tomato, with good retention of fresh aroma. In contrast, loss of compounds contributing to fresh green aroma ((E)-2-hexenal, 1-penten-3-one, 1-hexanol) and presence of heat-induced compounds (dimethyl sulfide, furfural, pyrrole derivatives) were detected in the spray-dried tomato.