Changing Temperature Conditions during Somatic Embryo Maturation Result in Pinus pinaster Plants with Altered Response to Heat Stress

Under the global warming scenario, obtaining plant material with improved tolerance to abiotic stresses is a challenge for afforestation programs. In this work, maritime pine (Pinus pinaster Aiton) plants were produced from somatic embryos matured at different temperatures (18, 23, or 28 °C, named after M18, M23, and M28, respectively) and after 2 years in the greenhouse a heat stress treatment (45 °C for 3 h/day for 10 days) was applied. Temperature variation during embryo development resulted in altered phenotypes (leaf histology, proline content, photosynthetic rates, and hormone profile) before and after stress. The thickness of chlorenchyma was initially larger in M28 plants, but was significantly reduced after heat stress, while increased in M18 plants. Irrespective of their origin, when these plants were subjected to a heat treatment, relative water content (RWC) and photosynthetic carbon assimilation rates were not significantly affected, although M18 plants increased net photosynthesis rate after 10 days recovery (tR). M18 plants showed proline contents that increased dramatically (2.4-fold) when subjected to heat stress, while proline contents remained unaffected in M23 and M28 plants. Heat stress significantly increased abscisic acid (ABA) content in the needles of maritime pine plants (1.4-, 3.6- and 1.9-fold in M18, M23, and M28 plants, respectively), while indole-3-acetic acid content only increased in needles from M23 plants. After the heat treatment, the total cytokinin contents of needles decreased significantly, particularly in M18 and M28 plants, although levels of active forms (cytokinin bases) did not change in M18 plants. In conclusion, our results suggest that maturation of maritime pine somatic embryos at lower temperature resulted in plants with better performance when subjected to subsequent high temperature stress, as demonstrated by faster and higher proline increase, lower increases in ABA levels, no reduction in active cytokinin, and a better net photosynthesis rate recovery.     

压力容器冷成形与热处理的界定

本文根据<压力容器安全技术监察规程>和GB150-1998<钢制压力容器>对压力容器冷加工成形的热处理要求,参照ASME最新版锅炉压力容器规范对这方面的规定作一综述,供同行在解决这方面问题时的参考.     

How Light Reactions of Photosynthesis in C4 Plants Are Optimized and Protected under High Light Conditions

Most C4 plants that naturally occur in tropical or subtropical climates, in high light environments, had to evolve a series of adaptations of photosynthesis that allowed them to grow under these conditions. In this review, we summarize mechanisms that ensure the balancing of energy distribution, counteract photoinhibition, and allow the dissipation of excess light energy. They secure effective electron transport in light reactions of photosynthesis, which will lead to the production of NADPH and ATP. Furthermore, a higher content of the cyclic electron transport components and an increase in ATP production are observed, which is necessary for the metabolism of C4 for effective assimilation of CO₂. Most of the data are provided by studies of the genus Flaveria, where species belonging to different metabolic subtypes and intermediate forms between C3 and C4 are present. All described mechanisms that function in mesophyll and bundle sheath chloroplasts, into which photosynthetic reactions are divided, may differ in metabolic subtypes as a result of the different organization of thylakoid membranes, as well as the different demand for ATP and NADPH. This indicates that C4 plants have plasticity in the utilization of pathways in which efficient use and dissipation of excitation energy are realized.     

Differential Physio-Biochemical and Metabolic Responses of Peanut (Arachis hypogaea L.) under Multiple Abiotic Stress Conditions

The frequency and severity of extreme climatic conditions such as drought, salinity, cold, and heat are increasing due to climate change. Moreover, in the field, plants are affected by multiple abiotic stresses simultaneously or sequentially. Thus, it is imperative to compare the effects of stress combinations on crop plants relative to individual stresses. This study investigated the differential regulation of physio-biochemical and metabolomics parameters in peanut (Arachis hypogaea L.) under individual (salt, drought, cold, and heat) and combined stress treatments using multivariate correlation analysis. The results showed that combined heat, salt, and drought stress compounds the stress effect of individual stresses. Combined stresses that included heat had the highest electrolyte leakage and lowest relative water content. Lipid peroxidation and chlorophyll contents did not significantly change under combined stresses. Biochemical parameters, such as free amino acids, polyphenol, starch, and sugars, significantly changed under combined stresses compared to individual stresses. Free amino acids increased under combined stresses that included heat; starch, sugars, and polyphenols increased under combined stresses that included drought; proline concentration increased under combined stresses that included salt. Metabolomics data that were obtained under different individual and combined stresses can be used to identify molecular phenotypes that are involved in the acclimation response of plants under changing abiotic stress conditions. Peanut metabolomics identified 160 metabolites, including amino acids, sugars, sugar alcohols, organic acids, fatty acids, sugar acids, and other organic compounds. Pathway enrichment analysis revealed that abiotic stresses significantly affected amino acid, amino sugar, and sugar metabolism. The stress treatments affected the metabolites that were associated with the tricarboxylic acid (TCA) and urea cycles and associated amino acid biosynthesis pathway intermediates. Principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and heatmap analysis identified potential marker metabolites (pinitol, malic acid, and xylopyranose) that were associated with abiotic stress combinations, which could be used in breeding efforts to develop peanut cultivars that are resilient to climate change. The study will also facilitate researchers to explore different stress indicators to identify resistant cultivars for future crop improvement programs.     

Genome-Wide Association Mapping Identifies New Candidate Genes for Cold Stress and Chilling Acclimation at Seedling Stage in Rice (Oryza sativa L.)

Rice (Oryza sativa L.) is a chilling-sensitive staple food crop, and thus, low temperature significantly affects rice growth and yield. Many studies have focused on the cold shock of rice although chilling acclimation is more likely to happen in the field. In this paper, a genome-wide association study (GWAS) was used to identify the genes that participated in cold stress and chilling accumulation. A total of 235 significantly associated single-nucleotide polymorphisms (SNPs) were identified. Among them, we detected 120 and 88 SNPs for the relative shoot fresh weight under cold stress and chilling acclimation, respectively. Furthermore, 11 and 12 quantitative trait loci (QTLs) were identified for cold stress and chilling acclimation, respectively, by integrating the co-localized SNPs. Interestingly, we identified 10 and 15 candidate genes in 11 and 12 QTLs involved in cold stress and chilling acclimation, respectively, and two new candidate genes (LOC_Os01g62410, LOC_Os12g24490) were obviously up-regulated under chilling acclimation. Furthermore, OsMYB3R-2 (LOC_Os01g62410) that encodes a R1R2R3 MYB gene was associated with cold tolerance, while a new C3HC4-type zinc finger protein-encoding gene LOC_Os12g24490 was found to function as a putative E3 ubiquitin-protein ligase in rice. Moreover, haplotype, distribution, and Wright’s fixation index (FST) of both genes showed that haplotype 3 of LOC_Os12g24490 is more stable in chilling acclimation, and the SNP (A > T) showed a difference in latitudinal distribution. FST analysis of SNPs in OsMYB3R-2 (LOC_Os01g62410) and LOC_Os12g24490 indicated that several SNPs were under selection in rice indica and japonica subspecies. This study provided new candidate genes in genetic improvement of chilling acclimation response in rice.     

Adipose Lipolysis Regulates Cardiac Glucose Uptake and Function in Mice under Cold Stress

The heart primarily uses fatty acids as energy substrates. Adipose lipolysis is a major source of fatty acids, particularly under stress conditions. In this study, we showed that mice with selective inactivation of the lipolytic coactivator comparative gene identification-58 (CGI-58) in adipose tissue (FAT-KO mice), relative to their littermate controls, had lower circulating FA levels in the fed and fasted states due to impaired adipose lipolysis. They preferentially utilized carbohydrates as energy fuels and were more insulin sensitive and glucose tolerant. Under cold stress, FAT-KO versus control mice had >10-fold increases in glucose uptake in the hearts but no increases in other tissues examined. Plasma concentrations of atrial natriuretic peptide and cardiac mRNAs for atrial and brain-type natriuretic peptides, two sensitive markers of cardiac remodeling, were also elevated. After one week of cold exposure, FAT-KO mice showed reduced cardiac expression of several mitochondrial oxidative phosphorylation proteins. After one month of cold exposure, hearts of these animals showed depressed functions, reduced SERCA2 protein, and increased proteins for MHC-β, collagen I proteins, Glut1, Glut4 and phospho-AMPK. Thus, CGI-58-dependent adipose lipolysis critically regulates cardiac metabolism and function, especially during cold adaptation. The adipose-heart axis may be targeted for the management of cardiac dysfunction.     

低温位废热回收与液化天然气冷能利用的集成研究

Two novel thermal cycles based on Brayton cycle and Rankine cycle are proposed, respectively, which integrate the recovery of low-level waste heat and Liquefied Nature Gas （LNG） cold energy utilization for power generation. Cascade utilization of energy is realized in the two thermal cycles, where low-level waste heat,low-temperature exergy and pressure exergy of LNG are utilized efficiently through the system synthesis. The simulations are carried out using the commercial Aspen Plus 10.2, and the results are analyzed. Compared with the conventional Brayton cycle and Rankine cycle, the two novel cycles bring 60.94% and 60% in exergy efficiency, respectively and 53.08% and 52.31% in thermal efficiency, respectively.     

太阳能热水相变炕体蓄放热性能及影响因素

提出一种太阳能热水相变蓄热炕的新型供暖系统，系统无须设置水箱，仅使用炕板与相变材料作为蓄热装置，可有效提高供暖效率。基于Fluent数值模拟平台，建立相变炕的二维非稳态传热模型，研究相变炕的蓄放热性能，并与混凝土炕的热性能进行对比；还分析了相变材料的相变温度和潜热对相变炕蓄放热性能的影响。结果表明，在设定工况下，与混凝土炕相比，上炕面的日间与夜间稳定温度分别提高2℃和4℃，上炕面最大温差由3.7℃降至0.8℃，全天得热量提高了66.36%。因此，相变炕具有上炕面温度较高、温度分布均匀、得热量大以及保温性能好等优点；提高相变温度，炕体得热量会有所减少，但对提升上炕面温度作用显著；增大相变潜热，可以显著提高炕体得热量，但对提升上炕面温度作用不明显。     

带亲水层条缝型翅片管换热器析湿工况空气侧换热与压降特性

对带亲水层条缝型翅片管换热器在析湿工况下的空气侧特性进行了实验研究.分析了翅片间距、管排数和入口相对湿度对空气侧换热及压降特性的影响.研究结果表明,当雷诺数较低时,翅片间距对空气侧换热特性的影响很小,当雷诺数大于1600时,空气侧换热特性随着翅片间距的增大而逐渐减小.翅片间距对空气侧压降特性的影响则同干工况相似.管排数对空气侧换热特性的影响非常明显,但对空气侧压降特性的影响则不明显.空气侧的换热性能随着入口空气相对湿度的增大稍微有所增强,而压降特性则几乎不受入口相对湿度的影响.开发了析湿工况下带亲水层条缝型翅片管换热器空气侧换热因子j和摩擦因子f 的关联式.平均误差分别为9.6%和7.8%,在±15%误差范围内分别能涵盖82.1%和89.3%实验数据.     

Leaf Photosynthesis and Its Temperature Response Are Different between Growth Stages and N Supplies in Rice Plants

Leaf photosynthesis is highly correlated with CO₂-diffusion capacities, which are determined by both leaf anatomical traits and environmental stimuli. In the present study, leaf photosynthetic rate (A), stomatal conductance (g_s), mesophyll conductance (g_m) and the related leaf anatomical traits were studied on rice plants at two growth stages and with two different N supplies, and the response of photosynthesis to temperature (T) was also studied. We found that g_m was significantly higher at mid-tillering stage and at high N treatment. The larger g_m was related to a larger chloroplast surface area facing intercellular air spaces and a thinner cell wall in comparison with booting stage and zero N treatment. At mid-tillering stage and at high N treatment, g_m showed a stronger temperature response. The modelling of the g_m-T relationships suggested that, in comparison with booting stage and zero N treatment, the stronger temperature response of g_m was related to the higher activation energy of the membrane at mid-tillering stage and at high N treatment. The findings in the present study can enhance our knowledge on the physiological and environmental determinants of photosynthesis.     

相变储能材料在温室大棚中应用研究进展

采用相变储热材料(PCMs)潜热储蓄技术是最有效热能贮存方式之一,将其应用于温室节能有重要意义.本文综述了低温相变储热材料在温室大棚中的应用条件及类型,国内外低温相变储热材料的研究现状.分析了温室储热材料的性能与存在问题,展望了低温相变储能材料的发展方向和应用前景.