Non-destructive, ultrasonic evaluation of meat quality in live Japanese Black steers from coloured images produced by a new ultrasound scanner

An improved colour scanning scope was used for evaluating meat quality (marbling) of live Japanese Black steers. This equipment consisted of a small size ultrasonic probe (2 MHZ) and LCD display. Seventeen fattened Japanese Black cattle were scanned at the region of the 7th rib about one week before slaughter. A picture of the cross-sectional area of the back was obtained immediately after applying the probe and contained 15 colours representing different signal strengths. The time for each scan was 2 seconds. The picture signals were fed into a computer for rapid estimation of fat percentage of the M. longissimus thoracis. After slaughter, the fat content and chemical characteristics were determined on the M. longissimus thoracis obtained from the same rib section. The range of fat content was 7.0 to 23.7% (average 18.47%). A high correlation coefficient (r = 0.90; r.s.d. = 2.01%) was obtained between actual fat percentage of the M. longissimus thoracis and colour-scanning scope SR200 estimates based on the percentage of the weak blue dot(1) in the echo. Estimates of the subcutaneous fat thickness and the cross-sectional area of M. longissimus thoracis from the scans were in good agreement with the actual carcass measurements (r = 0.69; r.s.d=0.52 cm and r = 0.81; r.s.d. = 4.26 cm(2), respectively). These results show that the new colour scanning scope is a useful instrument for estimating meat quality (marbling) in live cattle.     

Variation of the sensory quality within the m. longissimus thoracis et lumborum of PSE and normal pork

This study evaluated the variation in instrumental and sensory meat quality within the m. longissimus thoracis et lumborum of carcasses with normal and PSE meat. Out of 80 slaughtered pigs, respectively, 9 and 7 carcasses were selected with a pH30 value > or=5.80 (normal pork) and a pH30 value <5.80 (PSE pork). Instrumental and sensory meat quality measurements were performed at three locations on these carcasses: longissimus thoracis (LT), longissimus thoracis et lumborum: (LTL-mid-loin) and longissimus lumborum (LL). Meat of the thoracis site (LT) was paler, but more favourable for pH45, cooking loss, juiciness, tenderness and preference scores than meat of the mid-loin part. The lumborum site (LL) was more or less in between the other two locations for meat quality, but closer to the LTL than the LT site. The meat quality variation within the m. longissimus thoracis et lumborum followed a similar pattern for normal and PSE carcasses. It can be concluded that the mid-loin part is best suited as a reference place for meat quality assessment, if one wants to eliminate carcasses with unacceptable meat.     

Relationship between Japanese beef marbling standard and intramuscular lipid in the M. longissimus thoracis of Japanese Black and American Wagyu Cattle

Japanese Black and American Wagyu steers (n = 155) were slaughtered and graded under typical Japanese production conditions, and then analyzed for percentage ether-extractable fat at the 6th thoracic vertebra. The percentage of intramuscular lipid was used to develop a prediction equation for the Japanese beef marbling standard (BMS) with R(2) = 0·7619.     

Heterogeneous composition of histochemical fibre types in the different parts of M. longissimus thoracis from Mishima (Japanese native) steers

In Mishima (Japanese native) steers, histochemical properties of m. longissimus thoracis were examined at 3 parts: on the level of 6th thoracic (LT I), 11th thoracic (LT II) and 5th lumbar vertebra (LT III). Myofibres were categorized into Type I, II A and II B. The same fibre type composition (I, 37%, II A, 17%, II B, 46%) was observed at LT I and LT III and another (I, 26%, II A, 15%, II B, 59%) at LT II. At each part apparent regional differences of fibre type composition could not be demonstrated except for Type I between medial and lateral subpart at LT I and Type II A between central and lateral at LT III because of its marked variation among the individual steers. Relative fibre diameter of Type I to Type II A in the combined data was large at LT I and LT III. Type II B fibres showed larger diameter than Type I only at LT III. From these results it was suggested that Mishima steers have maintained another histochemical property of the longissimus muscle differing from that of Japanese Black steers (Gotoh, Iwamoto, Ono, Nishimura, Matsuo, Nakanishi, Umetsu & Takahara, (1994). Comparative study on the regional composition of fiber types in M. Longissimus thoracis with different marbling scores for Japanese Black steers. Animal Science and Technology, 65, 454-463).     

Growth and carcass characteristics of Angus and American Wagyu steers

Ten purebred Angus and ten crossbred (3/4–7/8) American Wagyu steers were fed a corn and barley-based diet for 552 days and slaughtered. All USDA and Japanese yield and quality grade factors were evaluated and the amount of extractable lipid and moisture in totally trimmed muscle was determined. Angus steers gained 0·9 kg/head/day and American Wagyus gained 0·7 kg/head/day. This difference in growth rate resulted in Angus steers having a heavier final weight (P < 0·05). Angus steers required less feed per unit of gain than did American Wagyu steers. Adjusted fat thickness over M. longissimus dorsi opposite the 12th rib was approximately 3·5 cm and was not statistically different between the two breeds (P > 0·05). Average ribeye area and kidney, pelvic and heart fat were similar for the two breeds. The calculated USDA yield grade for both breeds exceeded 6. Average USDA marbling score was nearly a degree of marbling higher for American Wagyu than for Angus, but variation within breed groups was high. Average USDA quality grade was well into USDA Prime for all carcasses and did not differ by breed (P > 0·05). Japanese yield grade factors were similar for both breeds except for cold left side weight and the yield estimation which includes an adjustment factor that favors American Wagyu. American Wagyu steers merited a higher Japanese marbling score than did Angus (P < 0·05). Beef color score, firmness, texture and firmness and texture grade were also different between the breeds (P < 0·05). Fat colour, luster and quality were not different (P > 0·05). These data clearly show that some American Wagyu steers have the genetic ability to deposit as much marbling as Japanese Black cattle raised in Japan.     

The characteristics of muscle fiber types of longissimus thoracis muscle and their influences on the quantity and quality of meat from Japanese Black steers

Forty-four Japanese Black fattening steers from four groups, produced in four districts and consisting of differing genetic backgrounds were slaughtered to examine the characteristic differences in muscle fiber types at the 6th thoracic vertebra of the M. longissimus thoracis (LT). The influence of percentage, diameter, and relative area of each muscle fiber type on the carcass characteristics and some quantity and quality traits of beef taken from LT, were also investigated. Significant differences in the characteristics of the muscle fiber types were observed among the four groups, except for muscle fiber diameter in the αR fiber, and the relative area of each αW fiber. For all steers, the average percentages and diameters of each muscle fiber type, βR, αR and αW were 26.8, 18.5 and 54.7% and 51.4, 50.6 and 52.4 μm, respectively. The relative area of each fiber type was similar to those of muscle fiber composition. αR Fiber content had significant negative correlations with marbling score (p<0.05), intramuscular fat content (p<0.05) and ultimate pH value (p<0.05). Significant correlations between the diameter of each fiber type, and the quantity or quality traits of the meat were not found, with the exception of red fiber types (βR and αR) and meat color a(?) values (p<0.05) which were positively correlated.     

THE SHEAR FORCE OF VERY-RAPIDLY CHILLED LAMB 2: THE EFFECT OF MUSCLE EXCISION

The effect of chilling rate and muscle excision on the shear force of ovine muscle (M. longissimus thoracis et lumborum) was examined. Conventional chilling involved chilling at 4C for 24 h and very-rapid chilling involved chilling at −20C for 3.5 h followed by conventional chilling for 20.5 h. After 24 h all muscles were aged for a further four days. Approximately 30 min after slaughter, one M. longissimus thoracis et lumborum was excised from each carcass and placed in the same chill as the remainder of the carcass. This gave a total of four different treatments. Shear force was assessed at I and 5 days postmortem by Warner-Bratzler shear force measurement. Excision led to reduced shear force in the very-rapid chilling regime but increased shear force in the conventionally chilled muscles. Aging improved shear force for all chilling treatments. No significant difference was found in the rate of pH fall, between the chilling treatments.     

Glycogen concentrations in bovine longissimus dorsi muscle

The aim of this study was to assess the effect of location within bovine longissimus dorsi (thoracis et lumborum) muscle (LTL) when determining glycogen concentration. Six locations in Angus heifers were sampled post mortem (EXP-1) and eight locations in live Charolais crossbred steers were biopsied (EXP-2). In EXP-1, there was more glycogen in the animals' left LTL (64.1±1.6 mmol/kg) versus the right LTL (57.0±1.6 mmol/kg) (P<0.05). Locations along the cranial-caudal axis within LTL did not differ in glycogen concentration. Results for EXP-2 did not confirm the sidedness effect. Instead, it showed that glycogen concentration was lower at the cranial sampling locations near the 10th rib (89±2.5 mmol/kg) than at the middle (97±2.0 mmol/kg) or caudal locations (96±1.9 mmol/kg) (P<0.005). Taking lactate accumulation into account (glycolytic potential) rendered those differences insignificant. Nevertheless, the tendency remained clear. The potential for dilution of glycogen by intramuscular fat deposits is discussed. The results indicate that one needs to be cautious in performing repeated sampling on bovine LTL.     

Predicting the weight of lean meat in lamb carcasses and the suitability of this characteristic as a basis for valuing carcasses

The carcasses of 138 lambs were dissected into fat, muscle and bone as the basis for developing a model to estimate the weight of lean meat (muscle and intramuscular fat). The lambs represented two sexes (70 wethers, 68 ewes) and three sire genotypes (67 Poll Dorset, 39 Suffolk, 32 Wiltshire Horn) all from Border Leicester × Polwarth × Booroola type ewes. Hot carcass weight (HWT) was found to explain the majority of the variation in the weight of lean meat. When measures of subcutaneous fat depth at different sites were used as predictors in addition to HWT, the accuracy with which lean meat yield could be estimated was found to increase by a small amount. There was, however, little difference in their individual value as predictors. The area of the M. longissimus thoracis et lumborum at the twelfth-thirteenth rib was found to account for the significant breed type difference between Poll Dorset and Suffolk sired lambs when included in a multiple regression with HWT and the GR measurement (tissue thickness at the twelfth rib 110 mm from the midline). The final model produced an R(2) = 0·92 and an RSD = 0·45 kg for the 106 lambs. Using the model for the 106 lambs, the estimated (from the model) and actual values of lean meat for the Wiltshire Horn sired lambs were compared. The correlation coefficient between the values was r = 0·97 and the RSD was 0·31 kg. This shows that for second cross lambs as used in this study the fitted model exhibits a degree of general validity and stability. An overall model for the 138 lambs produced an R(2) = 0·92 an RSD = 0·43 kg. The potential for pricing meat on the basis of lean meat yield is discussed, with particular emphasis on the current developments in assessment of lamb carcasses in Australia.     

Ether-Extractable Fat in Longissimus Muscles From Beef Carcasses with the Same Marbling Scores but Different Yield Grades

Ether-extractable fat in beef longissimus muscles–completely trimmed of all subcutaneous fat, epimysium and peripheral muscles–from 383 heifer and steer carcasses of A and B maturity was compared to determine whether the fat content of muscle was the same when marbling was held constant but yield grade of carcasses differed. Yield grade was a significant source of variation in intramuscular fat content of trimmed steaks within marbling scores (P < 0.001); as yield grade increased, total fat content of the ribeye muscle increased very slightly, even though marbling score was held constant. These data indicated that marbling score alone did not account for all of the variation in the fat content of totally-trimmed beef longissimus steaks.     

Cellular conditions for intramuscular fat deposition in Japanese Black and Holstein steers

The experiment was conducted to study the development of intramuscular fat in Japanese Black (JB) compared to Holstein (HS) steers and to find breed differences for fat depot development and distribution in the carcass under equal feeding conditions. Additional to slaughter samples, biopsy samples of longissimus muscle (LM) and subcutaneous fat, taken at 10, 14, 18, and 22 months of age, were used for histological and molecular investigations. Japanese Black steers stored about 14% more fat in the LM (P = 0.001), resulting in larger marbling flecks (P < 0.001). Muscle fibers and intramuscular adipocytes in both breeds responded to the high energy feeding with significant enlargement, which was faster in JB. Histograms of intramuscular adipocytes size showed a shift toward larger cells during growth, but also the abundance of small, developing adipocytes. This development was accompanied by a correlated up-regulation of adipogenic genes until 22 months of age.     

Ultrasonic scanning measurements of the longissimus thoracis et lumborum muscle to predict carcass muscle content in sheep

Nineteen Omani sheep (average 26 kg body weight) were scanned over the 6th rib, 12th rib and the second-last lumbar vertebra, to determine the maximum depth (B), circumference, area of the cross-section and volume of the longissimus thoracis et lumborum (LD) muscle. The volume of the muscle was estimated by multiplying the average area of its cross-section obtained at the three sites by its length. After slaughter, the LD muscle was dissected out, frozen, and its maximum depth, circumference, length, weight and volume were determined. Correlation and regression analyses were carried out to evaluate relationships between scanning and real measurements and between scanning measurements and carcass total muscle content. Generally there were positive correlations (r = 0.43-0.66) between the LD scanning measurements made at the 12th rib and the corresponding measurements made on the dissected muscle. The volume of the LD muscle estimated from scanning was positively correlated (r = 0.59) with its real volume which was correlated with its weight (r = 0.51) and consequently with the total carcass muscle content (r = 0.69). Ultrasonic scanning can be used to predict the circumference and volume of the LD muscle. These may be used for prediction of total carcass muscle content in sheep in addition to traditional measurements of the muscle (the maximum depth B) and area.     

Recommended terminology for the muscle commonly designated 'longissimus dorsi'

Musculus longissimus of the erector spinae is frequently used by meat scientists because of its relative mass, its commercial importance and its variability in quality. Its designation has fluctuated considerably due to confusion over interpretation of accepted anatomical guidelines, some of which have been altered to improve precision. We recommend that meat and muscle scientists refer to the part of the whole muscle they generally use as the Longissimus thoracis et lumborum (LTL) or to either of its two parts, Longissimus thoracis (LT) or longissimus lumborum (LL), depending on which is referenced. The word dorsi should be omitted, the word musculus (or its abbreviation M.) preceding all muscles in Latin should not be used in English, and the longissimus atlantis, capitis and cervicis should not be included except when they are actually used.     

Prediction of carcass composition and individual carcass cuts of Japanese Black steers

The objective of this study was to develop equations to predict carcass tissue weights and percentages and boneless carcass non-trimmed cut weights by using the cold carcass weight (CCW) and three other traits at the 6–7th rib section, which are routinely collected in carcass markets in Japan. Carcasses from 94 Japanese Black steers were used for the multiple regression analysis with a stepwise procedure and a novel Least Absolute Shrinkage and Selection Operator (LASSO). The accuracies of prediction (R²) and RMSEs for the carcass tissue and cut weights were similar between the two procedures. In contrast, LASSO appeared to be the better procedure for predicting carcass tissue percentages. The longissimus muscle area and subcutaneous fat thickness were the important predictors for the lean percentage in the stepwise procedure, and CCW was additionally selected when the LASSO procedure was used.     

Comparisons of the effect of electrical stimulation methods on postmortem pH decline in beef muscle

Results of four electrical stimulation (ES) studies were summarized to demonstrate the impact of different ES parameters on pH decline patterns in postmortem M. longissimus thoracis et lumborum. Postmortem pH decline was modeled as a non-linear function of time, and estimates of minimum obtainable pH, pH decline rates, and time to reach pH 6·0 were compared for each study. The decline model for study 4 (AC, 60 Hz, 50 V; 5 min post mortem) had a larger (P < 0·05) estimate for decline rate than that for study 1 (AC, 60 Hz, 400 V; 1 h post mortem) and the control (non-stimulated) data. The model estimate of time to pH 6·0 (0·56 h) for study 4 was the shortest (P < 0·05) for all treatments. Different ES parameters produce different pH decline patterns post mortem and, therefore, may impact product quality and fabrication and chilling protocols adopted in fresh beef processing.     

OXYMYOGLOBIN IN BOVINE MUSCLE SYSTEMS AS AFFECTED BY OXIDIZING LIPIDS, VITAMIN E AND METMYOGLOBIN REDUCTASE ACTIVITY

Oxymyoglobin and lipid oxidation were examined in low and high vitamin E (α-tocopherol) bovine M. longissimus thoracis et lumborum (LD) homogenates and in subcellular fractions of LD. Investigation of the effects of temperature (4, 22, 37C) and pH (5.5, 6.4, 7.2) on lipid and oxymyoglobin oxidation indicated that oxymyoglobin oxidation increased with increasing temperature (P < 0.05) and was higher at the low pH (P < 0.05). Lipid oxidation increased with temperature (P < 0.05) only at pH 5.5. The inclusion of 45 μM Fecl₃/ascorbate (1:1), at pH 5.5 and 4C, led to significant increases (P < 0.05) in lipid oxidation but significant oxymyoglobin oxidation occurred only when the extent of lipid oxidation exceeded a threshold level. Oxymyoglobin and lipid oxidation were significantly lower in LD fractions derived from high vitamin E muscle compared to low vitamin E muscle. α-Tocopherol did not interact directly with oxymyoglobin to suppress oxymyoglobin oxidation but a positive unit effect of muscle α-tocopherol on metmyoglobin reductase activity was observed.     

Estimating beef carcass composition using the speed of ultrasound

The reciprocal of the speed of 2·25 MHz (nominal) ultrasound (RV) was measured at selected sites through the soft tissues of 72 beef carcasses from Friesian. Hereford and Hereford × Friesian bulls and steers at or within 1 h of stunning. The measurement sites were: through M. adductor horizontal to the caudal edge of the symphysis pubis (S2), through M. scalenus just cranial to the first rib (S3) and through M. longissimus thoracis between the tenth and eleventh ribs, approximately 6 cm from the midline (S4). The thickness of extractable lipid (d(f)) at each site was defined as the product βd, where β was the volume fraction of extractable lipid and d was the tissue thickness. The thickness of lipid-free tissue (d(ff)) was defined as (d-d(f)) and β was calculated from the ultrasonic measurements using an empirical linear relation between β and RV. When cold, the intact sides were scored independently for fatness and conformation by two experienced judges according to the EAAP system and their mean scores determined. Sides were split at the level of the last rib and the depth of subcutaneous fat measured at 25%, 50% and 75% of the width of M. longissimus thoracis from the midline. The three ultrasonic characteristics (RV, d(f) and d(ff)) at each site, the mean fat depth and the mean fat and conformation scores were examined for correlation with % fat and % lean in the sides determined by complete physical dissection. The best predictors of % fat were mean d(f) at S3 and S4, mean d(f) at S2, S3 and S4 and mean EAAP fat score which were all of comparable precision (rsd, respectively, 2·07, 2·06 and 2·08). The best predictors of % muscle were mean d(f) at S3 and S4, mean d(f) at S2, S3 and S4 and mean RV at S2, S3 and S4 (rsd, respectively, 1·79, 1·79 and 1·90). The corresponding residual standard deviations for mean EAAP fat score and mean fat depth were 2·18 and 2·20.     

The effect of dietary poultry litter supplementation on beef chemical, cooking, and palatability properties and consumer acceptance

Twenty Holstein steers were randomly and equally allocated to two groups (treatment and control) at a mean weight of 334.3 kg and were backgrounded for 133 days and fed a finishing diet for 99 days under commercial conditions. Control animals received 97.1% corn silage, 2.6% canola meal, and 0.3% mineral mix during backgrounding and 72.6% corn silage, 3.9% canola meat, 23% barley and 0.2% mineral mix during finishing. Treated animals received 95.3% corn silage, 4.4% poultry litter, and 0.3% mineral mix during backgrounding and 62.1% corn silage, 5.6% poultry litter, 22% barley, and 0.2% mineral mix during finishing. The poultry litter was provided as a pelleted. At the end of the finishing period, all animals were harvested. After 144 h at 2 °C, the boneless longissimus thoracis et lumborum muscles were removed, cut into steaks (1.9 cm thick) and roasts, vacuum packaged, and frozen. A steak adjacent to the 13th thoracis vertebra of alternate carcass sides was displayed under simulated retail conditions for 4 days and then analyzed for moisture and fat content. A steak immediately posterior to the 13th thoracis vertebra of alternate carcass sides was evaluated by an experienced trained six-member laboratory panel for palatability attributes. The steak immediately posterior to the steak used for sensory evaluation was used for shear force evaluation. A roast constituting the reminder of the longissimus lumborum muscle was used to obtain complete flavour and texture profiles. Total cooking losses were determined and cooking times were recorded for both steaks and roasts. All remaining steaks (1180) were distributed at random to households to determine consumer acceptance. Treated samples were essentially indistinguishable from control samples in both palatability and consumer acceptance, indicating poultry litter supplements can be utilized in feedlot rations for cattle without compromising either palatability or consumer acceptance.     

The value of carcass weight, fat depth measures and eye muscle area for predicting the percentage yield of saleable meat in Australian grass-fed beef carcasses for Japan

The percentage saleable meat yield was determined for 42 carcasses from grass-fed steers representing a range of breed types purchased in Australia for the Japanese market. Their mean (s.d.) carcass weight and P8 fat depth were 329 (28.1) kg and 17.3 (4.3) mm, respectively. All measures of subcutaneous fat depth were significantly (P < 0.05) and moderately correlated with percentage saleable meat yield, with measures in the loin region showing a stronger association than those taken in the rump region. The association between P8 fat depth and the loin measures of subcutaneous fat were low and there was a significant (P < 0.05) association only between P8 and fat depth at the 10th rib (FD 10). The use of fat depth measurements from the loin region reduced the error associated with predicting saleable meat yield more than those from the rump region and significantly increased the amount of variation (R(2)) in saleable meat yield that was explained. Hot carcass weight (HCW) did not significantly (P > 0.05) improve prediction models when combined with subcutaneous fat depth measurements and overall, the R(2) values were low ranging from 0.19 to 0.42. The models indicated that fat depth measures and carcass weight are poor predictors of percentage meat yield in heavy-weight carcasses from mixed breed cattle as produced and processed in Australia. The prediction of percentage yield was in general significantly (P < 0.05) improved when measures of M. longissimus thoracis et lumborum (LD) area were added as independent variables to models based on hot carcass weight and subcutaneous fat depth measurements. With LD area added the amount of variation in yield that could be explained by the models increased by as much as 28%. Overall, the best model was based on fat depth at the 12th rib and LD area measured at the 5th rib for which the R(2) was 0.58 and the residual standard deviation was 1.63%. The next most accurate prediction of yield was provided by a model which included the independent variables used in the Australian Chiller Assessment Scheme namely HCW, FD10 and LD area at the 10th rib upon which breed type had no significant (P > 0.05) effect.     

延迟冷却对牛肉品质及超微结构影响的研究

本文研究了常规冷却与延迟冷却对中国杂交黄牛(鲁西黄牛×西门塔尔)宰后冷却过程中牛胴体pH值、温度变化、胴体冷却失重、肉色及成熟过程中剪切力值、背最长肌汁液损失、蒸煮损失、保水性、肌原纤维结构变化的影响.结果表明延迟冷却能减缓牛胴体温度的下降速率(P＜0.05),加快牛胴体pH值的降低(P＜0.05),提高牛背最长肌的嫩化速率,改善肉色(P＜0.05),但加剧了汁液损失、蒸煮损失,降低了保水性(P＜0.05).延迟冷却对成熟0d、3d时的肌原纤维结构影响显著(P＜0.05),延迟冷却组的肌节长度明显长于对照组的肌节长度.