A Method for Fabrication of Polycarbonate-Based Bioactive Platforms

Surface-based assays have been used extensively for the functional and structural analysis of biomolecules such as DNA or proteins. These experiments are established by the analysis of binding between acceptor molecules and immobilized receptors on a platform. Site-specific printing of receptor molecules on gold, glass, or polycarbonate (PC) surfaces is conventionally performed by the chemical derivatization of a surface, priming it to covalently bind to subsequently deposited receptor molecules. Unlike conventional methods, we have developed a new fabrication method for bioactive PC surfaces by directly molding PC granules doped with receptor molecules. PC-based receptor molecules were synthesized and commercially available PC granules were doped with these synthesized molecules. In our proof-of-concept study, PC doped with dye 1 (Fig. 1) was used as the receptor molecule. Using an aluminum mold and a hot press machine, PC-based objects were manufactured through compression molding using doped PC granules. Affinity analysis was evaluated by monitoring the localization of a monoclonal antibody elicited against dye 1 to the surface of the molded platforms by fluorescence microscopy. The results illustrated effective binding of an anti-dye 1 monoclonal antibody to the surface, substantiating successful display of assemblies of molecular receptors on the surface through compression molding. Although conventional surface functionalization methods impose limited applications and alter desired opto-mechanical properties of the polymer, our investigation provides a versatile means for the fabrication of bioactive PC-based platforms. It can also be used for engineering and imbedding receptor arrays within three-dimensional objects with applications to the production of opto-medical devices or biosensors.     

Implementation of Proteomics in Clinical Trials

The application of protein (or peptide) biomarkers in clinical studies is a dynamic, ever‐growing field. The introduction of clinical proteomics/peptidomics, such as mass spectrometry–based assays and multiplexed antibody–based protein arrays, has reshaped the landscape of biomarker identification and validation, allowing the discovery of novel biomarkers at an unprecedented rate and reliability. To reflect the current status with respect to implementation of protein/peptide biomarkers, an investigation of the most recent (last 6 years) clinical studies from clinicaltrials.gov is presented. Forty‐two clinical trials involving the direct use of protein or peptide biomarkers in patient stratification and/or disease diagnosis and prognosis are highlighted. Most of the clinical trials that include proteomics/protein assays are aiming toward implementation of non‐invasive diagnostic tools for early detection, while many of the clinical trials are targeting to correlate the protein abundance with the risk of a disease event. Less in number are the studies in which the protein biomarkers are applied to stratify the patients for intervention. All the above areas of application are considered of great importance for improving disease management, in an era where implementation toward precision medicine is the desired outcome of proteomics biomarker research.     

Optimizing Kinase Assays for Ultrahigh-Throughput Profiling Using the Kinase-Glo Plus Assay

Kinase profiling is an important component of the drug discovery process. Profiling with IC50 provides simultaneous potency and selectivity information about compounds of interest. Profiling data assist in the detection of inhibitors that may have potential toxic effects due to off-target activity. Compounds that exhibit the strongest potency and specificity profiles can be identified as leads for further optimization. Miniaturizing this profiling process in uHTS format generates data from a wide range of compound concentrations, providing more meaningful and accurate information. The combination of the Kinase-Glo Plus Assay with low-volume liquid-dispensing equipment provides an excellent solution for assay optimization and kinase profiling.     

A multi-scale electrode array (MSEA) to study excitation-contraction coupling of cardiomyocytes for high-throughput bioassays

Excitation-contraction coupling, a process of linking electrical activity to contraction, is the basis of cardiac function at cellular level. Here we developed a multi-scale electrode array (MSEA) as an in vitro platform to synchronously monitor excitation and contraction of cultured cardiomyocytes. The electrodes of MSEA were with sizes ranging from several tens of micrometers to about 1 mm and were classified into type_1, type_2 and type_3 by their electrode area. The former two types of electrodes were electroplated with platinum black to reduce baseline noise and no extra treatment was done on type_3 electrodes. In experiment, the micrometer-scale electrodes recorded extracellular field potentials like generally used multielectrode arrays and especially, the millimeter-scale electrodes recorded a “hump” followed after extracellular field potential. By analyzing temporal behavior of hump and observing its change under treatment of microtubule inhibitor vinblastine, it was verified that the hump expressed mechanical contraction of cardiomyocytes. In addition, we analyzed that the pathway of contraction recording was through cell-electrode contractile coupling, in which charges at electrode-electrolyte interface were reestablished due to contractile behavior of cellular layer and then the hump was formed. Finally, the dependence of amplitude and time duration of hump on electrode area, electrode property and contractile strength was also explored.     

高精度高压高阻检定仪设计

针对工程中对高压高阻检定精度不断提高的问题,设计了一套基于高压电桥法和ARM的数字式高压高阻鉴定系统。该系统硬件部分主要包括高压电源模块、高压电桥模块、信号采集模块、ARM cortex M4嵌入式控制模块。在控制模块设置测量电压,将高压电源模块输出电压加载到被测电阻两端,根据量程选择恒流恒压方式进行测量,经过A/D采集计算出所测量的电阻,同时可以通过LCD多级菜单设置测量系数并显示鉴定结果。本系统将电桥测量原理、数字处理技术结合在一起,实现了对目前国内市场上各种电压、电阻范围高阻箱的检定。经过试验表明鉴定结果符合JJG 166-93、DL/T 979-2005,可替代传统的高压高阻电桥。     

高速高效高品质——Fujitsu(富士通)发布全新fi-6系列高速文档图像扫描仪

作为高速扫描仪领域的领跑者,Fujitsu(富士通)正式发布栓线产品-6-6系列高速文档图像扫描仪.fi-6系列涵盖fi-6010N、fi-6130/fi-6230、fi-6140/fi-6240、fi-6670/fi-6670A、fi-6770/fi-6770A、fi-6750s多款新品,以海量文档扫描、人性化设计和网络管理应用为基础,秉持高品质的产品理念,以简单易用、高效扫描速度、逼真写实的图像处理能力,在降低办公成本、提升工作效能、加速企业文档信息化进程方面提供最新最全面的解决方案,以「高速高效高品质」再次成为业界领袖.……     

高速 高效 高品质——Fujitsu(富士通)发布全新fi-6系列高速文档图像扫描仪

作为高速扫描仪领域的领跑者,Fujitsu(富士通)正式发布全线产品--fi-6系列高速文档图像扫描仪。fi-6系列涵盖fi-6010N、fi-6130/fi-6230、fi-6140/fi-6240、fi-6670/fi-6670A、fi-6770/fi-6770A、fi-6750s多款新品,以海量文档扫描、人性化设计和网络管理应用为基础,秉     

高速高精度ADC系统研究

由于单个ADC模拟输入带宽的限制,混合滤波器组ADC系统不能够直接对高达数GHz的模拟信号进行采样.本文提出了基于混频器和低通滤波器的抽取器数学模型,并且从时域和频域进行详细的分析和证明.最后在混合滤波器组ADC系统的基础上,提出了基于混频器和低通滤波器的高速高精度混合滤波器组ADC系统,它完全满足软件无线电和雷达等多个领城的高速高精度要求.     

高效率高耐力 黑白打印

理光Aficio MP 6002/7502/9002数码黑白多功能复合机上市当今社会是一个信息爆炸的时代,在激烈商战中的决胜之道,就是如何高效处理文档信息,掌握商务先机。如今,一款具备更大生产力、更持久的耐力、更先进的工作流程优化的输出设备将成为简易高效办公的好帮手。作为理光炫睿系列产品的新成员,理光Aficio MP 6002/7502/9002数码黑     

看得见的高清

高清，一个很高调的字眼就像挂在最高枝头的一枚青苹果让我们可望而不可及。总是以为，高清离我们还很遥远，总是以为，高清需要高成本投入才能一亲芳泽……其实……潘多拉的盒子一旦打开，金刚、蜘蛛侠、变形金刚、哈里波特……就会不停地从盒子里飞出来，电脑上、碟机里，甚至游戏机里，你都可以觅到他的行踪。     

大功率高压变频器的选型

对大功率变频装置的结构、技术性能、控制方式及适用工况进行分析,并就其工程实施及合理选配进行探讨.     

玩心情的建筑

净化现代生活环境的本质,奥地利的摄影师成功做到了。约瑟夫·舒尔茨（Josef Schulz）拍出来的照片是双重曝光的,仿佛只是自然地拍下了仓库和办公楼,但实际上是经过处理的。     

基于JAVA平台创建高性能与高伸缩性的WebMail系统

基于Java平台的大容量WebMail系统的设计和实现方法,介绍了用JSP实现WebMail时的关键技术,在实际应用中达到较好的效果。     

高空间分辨率、高灵敏度微波辐射计研究

简述了进行高空间分辫率，高灵教度徽波栖射计研究的必要性.根据徽波辐封计天线接收地物徽波福封的特性，讨论了三种提高徽波格封计空间分辫率的新技术:大口径天线技术、合成孔径技术、等效口径技术.为了适应未来星载徽波辐升计新型扫描方式的要求，提出了结构简单、高德定、高灵教度的徽波格封计方案-计算机增益波动自动补偿徽波辐升计。