明胶在创伤敷料中的应用

明胶因其具有良好的理化性能被广泛地应用于组织工程学,如皮肤代用品、骨代用品等等,本文专就其在创伤敷料中的应用进行了综述。     

CA文摘

有切割标志的喷墨记录纸的制造,含有辐照敏感剂的水凝胶创伤敷料及制备方法,含疏水有机材料的轻型的，吸水的培养土替代品的制造,用PVA和／或PEG大分子混合物制造的生物可降解盒生物相容的交联聚合物水凝胶,具有良好的透明度和氧气屏蔽性的气体屏蔽薄片,好的粘合性和抗煮和曲折的多层膜和它们的薄片,     

壳聚糖基纳米复合材料在创伤修复的研究进展

综述了近年来通过静电纺丝、超声处理等方法制得的壳聚糖基纳米复合材料及其在创伤修复、抗菌领域中的应用新进展。分析了新颖的纳米生物材料对创面修复及保护伤口感染方面的潜在应用,还主要介绍了壳聚糖基纳米复合材料作为伤口敷料的应用研究现状并对其发展前景进行展望,为进一步的研究开发提供参考。     

氧化石墨烯在生物医用材料中的应用研究

介绍了氧化石墨烯的结构、性能,综述了关于氧化石墨烯的细胞毒性、生物相容性的研究,概述了氧化石墨烯在胶原基复合材料、胶原基组织工程支架材料以及胶原基创伤敷料等生物医用材料中的应用,展望了其发展前景。     

壳聚糖基复合水凝胶在创伤修复中的应用

综述了近年来壳聚糖与温敏材料、生长因子、多肽及多种其他材料通过物理或化学方法复合制备的壳聚糖基水凝胶及其在创伤修复领域中研究和应用的新进展,并对其发展前景进行了展望,期望为后续壳聚糖基水凝胶的进一步研究提供参考。     

壳聚糖基复合水凝胶在创伤修复中的应用

综述了近年来壳聚糖与温敏材料、生长因子、多肽及多种其他材料通过物理或化学方法复合制备的壳聚糖基水凝胶及其在创伤修复领域中研究和应用的新进展,并对其发展前景进行了展望,期望为后续壳聚糖基水凝胶的进一步研究提供参考。     

静电纺丝制备生物医用敷料研究进展

介绍了静电纺丝的原理以及静电纺丝纤维膜作为医用敷料的优点;综述了国内外静电纺丝制备医用敷料的研究进展,包括用于体外创伤护理、皮肤再生和具有定向药物释放功能的静电纺丝医用敷料。指出静电纺丝纳米纤维医用敷料作为一种高科技的功能性敷料,具有良好的发展前景。     

明胶基海绵的研究

制备和比较明胶／藻酸盐(GA)，明胶／透明质酸盐(GH)和壳聚糖／透明质酸盐(CH)交联海绵，含有或不含有磺胺嘧啶银(AgSD)的6种不同海绵应用于治疗背部全厚度皮肤损伤的Wistar老鼠。这种皮肤损伤的组织学和表皮伤口愈合速度的研究是借助光学显微镜和计算机的形态测量学5d和12d后的后期工作。在对我们的全厚度损伤试样(直径1cm)的治疗中，总体上说，含AgSD的海绵具有优良的伤口愈合能力。然而，明胶基海绵(GA，GH)和CH的伤口愈合的区别显得更有意义。通过对组织学研究结果和计算机化的形态测量学的表皮愈合分析发现，含AgSD的GH作为创伤敷料具有最强的伤口愈合能力。     

海洋多糖壳聚糖及其衍生物在创伤修复中的应用

壳聚糖作为世界上最丰富的天然生物材料之一,其创伤修复的特性在医用敷料领域引起人们极大的关注。介绍了壳聚糖及其衍生物创伤修复机理以及应用,为壳聚糖及其衍生物作为医用敷料的研究应用提供理论指导。     

铬革屑的高值利用研究

采用分段从铬革屑中提取胶原蛋白的方法。制得铬含量和灰分含量分别低于2mg/kg和2％，平均分子量为85kD的胶原蛋白。以此胶原蛋白为主要成分制备了胶原基创伤敷料。该敷料的物理机械性能、透气性以及与创伤的贴附性能良好，功能性评价结果表明：该敷料与动物皮肤相容性好，无刺激反应。有明显的保护伤口、防止感染和促进伤口愈合的作用。     

Procoagulant and Antimicrobial Effects of Chitosan in Wound Healing

Chitosan, a polysaccharide derived from chitin, has excellent wound healing properties, including intrinsic antimicrobial and hemostatic activities. This study investigated the effectiveness of chitosan dressing and compared it with that of regular gauze dressing in controlling clinically surgical bleeding wounds and profiled the community structure of the microbiota affected by these treatments. The dressings were evaluated based on biocompatibility, blood coagulation factors in rat, as well as antimicrobial and procoagulant activities, and the microbial phylogenetic profile in patients with abdominal surgical wounds. The chitosan dressing exhibited a uniformly fibrous morphology with a large surface area and good biocompatibility. Compared to regular gauze dressing, the chitosan dressing accelerated platelet aggregation, indicated by the lower ratio of prothrombin time and activated partial thromboplastin time, and had outstanding blood absorption ability. Adenosine triphosphate assay results revealed that the chitosan dressing inhibited bacterial growth up to 8 d post-surgery. Moreover, 16S rRNA-based sequencing revealed that the chitosan dressing effectively protected the wound from microbial infection and promoted the growth of probiotic microbes, thereby improving skin immunity and promoting wound healing. Our findings suggest that chitosan dressing is an effective antimicrobial and procoagulant and promotes wound repair by providing a suitable environment for beneficial microbiota.     

Advances in wound dressing based on electrospinning nanofibers

In recent years, there has been a significant focus on bioactive dressings suitable for treating chronic and acute wounds. Electrospinning nanofibers are considered advanced dressing options due to their high porosity and permeability to air and water, effective barrier properties against external pathogens, and excellent resemblance to the extracellular matrix for wound healing and skin regeneration. This article reviews the recent advancements in the application of electrospinning nanofibers for bioactive wound healing. The review begins with an overview of the wound healing process and electrospinning methods. It then explores the advantages and disadvantages of different synthetic and natural polymers used in the preparation of electrospinning wound dressings. The natural polymers discussed in this review include collagen, gelatin, silk fibroin, chitosan, hyaluronic acid, and sodium alginate. Additionally, the review delves into commonly used synthetic polymers like polyvinyl alcohol, polyvinyl chloride, polyethylene lactone, polylactide, and polyurethane for wound dressing applications. Furthermore, the review examines the blending of natural and synthetic polymers to create high-performance wound dressings. It also explores the incorporation of functional additives, such as antimicrobial agents, growth factors, and natural extracts, into electrospinning nanofibers to expedite wound healing and tissue repair. In conclusion, electrospinning is an emerging technology that provides unique opportunities for designing more effective wound dressings and care products.     

双负载抗生素亲水性聚氨酯泡沫的制备与表征

因细菌、异物和炎症等因素引起的慢性伤口会有过量的伤口渗出液流出,影响伤口周围健康皮肤,不利于伤口愈合.针对以上问题,我们开发了双负载亲、疏水性抗生素的亲水性聚氨酯泡沫作为慢性伤口的创面敷料.通过电子显微镜观察,泡沫的孔径均匀地分布在200～400 μm之间.聚氨酯泡沫中载入的药物对聚氨酯泡沫的力学性能略有影响,对吸液率...     

Nitrofurazone‐loaded PVA–PEG semi‐IPN for application as hydrogel dressing for normal and burn wounds

Hydrogels have been used in a wide variety of biomedical devises, particularly in the field of drug delivery, tissue engineering, and wound healing. In this study, a polyvinyl alcohol (PVA)–polyethylene glycol (PEG) semi‐interpenetrating hydrogel network (IPN)‐based wound dressing system containing nitrofurazone (NFZ) was synthesized by chemical crosslinking technique. The introduction of PEG to PVA matrix led to reduction in the water vapor transmission rate, which in‐turn resulted in improved healing activity. Drug‐loaded IPNs were prepared by mixing aqueous solution of NFZ with the optimized PVA–PEG formulation subsequent to the crosslinking step. The in vitro diffusion studies of NFZ indicated a relatively slow release of drug resulting from its microencapsulation in the polymeric matrix. Subsequently, in vivo wound healing efficacy toward acute and burn wound healing in experimental rats was investigated. Semi‐IPN hydrogel loaded with NFZ dressing improved the overall healing rate in both acute and burn wounds, as evidenced by significant increase in total protein, hydroxyproline and hexosamine contents. Histological examinations also correlated well with the biochemical findings. A faster wound contraction was also observed in hydrogel treated acute and burn wounds. The results indicated that PVA–PEG semi‐IPN hydrogel based dressing systems containing NFZ could be used as an effective wound dressing material. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013     

A Novel Route for the Preparation of Silver Loaded Polyvinyl Alcohol Nanogels for Wound Care Systems

This study is aimed at the development of a composite wound dressing containing nanosilver loaded polyvinyl alcohol (PVA) nanogels. The PVA nanogels were prepared by fructose induced reduction of silver nitrate within PVA gel to develop nanosilver nanogel (nGel). The influence of various synthesis parameters on PVA nanogel formation was investigated. The particle size of the nanogels, as evaluated by transmission electron microscopy was observed to be in the range of 10–50 nm. Glycerol was added to the nGel system and this composition, nGel/Glc, was coated on cotton fabric to develop a composite wound dressing. The cumulative release of silver from the dressing was found to be ～36% of the total loading after 48 h. Even at such low concentrations, high antibacterial efficiency was achieved against both gram positive and gram negative bacteria. In vivo wound healing studies were carried out over a period of 21 days on full-thickness skin wounds created on Swiss albino mice. Fast healing was observed in nGel/Glc treated wounds with minimum scarring, as compared to other groups. These results suggest that nGel/Glc based dressing material could be promising candidates for wound dressing applications.     

Comparative study of the properties of sericin-gelatin nanofibrous wound dressing containing halloysite nanotubes loaded with zinc and copper ions

Abstract

This study introduces and characterizes a novel antibacterial wound dressing from gelatin-sericin nanofibers containing halloysite nanotubes (HNTs) loaded with therapeutic copper and zinc ions. Finer fibers with faster bactericidal activity were observed in the Cu²⁺ containing mat; however, the fibroblast viability was reduced in these samples. On the contrary, Zn⁺² loaded nanofibers exhibited the best hosting area for fibroblast cells attachment, viability, and collagen secretion. Therefore, the proliferative effects of Zn-loaded gelatin-sericin nanofibers along with sufficient mechanical properties and favorable antibacterial activity proved that such a nanocomposite can have a potential of use as an antibacterial wound dressing.     

In vitro degradation and drug‐release behavior of electrospun,fibrous webs of poly(lactic‐co‐glycolic acid)

Ultrafine fibrous webs of poly(lactide‐co‐glycolic acid) (PLGA) containing the bactericidal antibiotic drug rifampin were prepared by electrospinning, and their properties were investigated for wound‐dressing applications. Because PLGA is a biodegradable and biocompatible polymer, it is one of the best materials for the preparation of wound‐dressing substrates. Through this investigation of PLGA/rifampin electrospun webs, we found that the in vitro degradation reached approximately 60% in 10 days, and the drug release from the webs showed a fast and constant profile suitable for wound‐dressing applications. Also, we observed that both the web‐degradation rate and the drug‐release rate increased as the drug concentration in the PLGA/rifampin electrospun webs and the content level of glycolide units in the PLGA polymer matrix increased. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012     

Preparation of hybrid PU/PCL fibers from steviol glycosides via electrospinning as a potential wound dressing materials

In this study, the synthesis and application of biocompatible steviol glycosides based polyurethane/poly (ε-caprolactone) (PU/PCL) fibers was performed by electrospinning as a potential wound dressing materials that can be used for the closure of nonhealing wounds. During electrospinning, steviol glycoside-based polyurethane structures were used in blend formation with poly (ε-caprolactone) for easy producibility. Steviol glycosides are a natural abundant and easily accessible source as the main component of the wound dressing material due to their free hydroxyl groups, high biocompatibility, and hydrophilicity. The structure of steviol glycosides is composed of saccharide units and the free OH groups. Thus, steviol glycosides act as a crosslinker within the polyurethane structure and provides mechanical strength. For the production of steviol glycosides based PU/PCL fibers first, the steviol glycosides as a monomer were isolated from the stevia rebudiana. Then, polyurethane structures containing stevia glycoside were synthesized with hexamethylene diisocyanate, lactose and PEG-200 by solution polymerization technique. PCL was added to the prepared polyurethanes in a ratio of 1:2 and formation of nanofiber structure. The prepared wound dressing material was characterized by Fourier transform infrared, atomic force microscopy, and scanning electron microscope techniques. Swelling degree, water content and oxygen permeability assay of the steviol glycosides based PU/PCL wound dressing material was determined. In biocompatibility test, cell viability value of PU/PCL fibrous materials in indirect cytotoxicity test was determined as 86.9% and cell adhesion on hybrid PU/PCL fibers was showed as morphological. In accordance with this target, the steviol glycosides based PU/PCL wound dressing material can be produced easily and low cost. As a result, the wound dressing materials obtained with their high biocompatibility and low costs will be an effective and fast method for the healing of open wounds of diabetics.     

Fibro‐porous polycaprolactone membrane containing extracts of Biophytum sensitivum: A prospective antibacterial wound dressing

A fibro‐porous wound dressing with antibacterial activity was fabricated from polycaprolactone (PCL) solution containing crude extract of biophytum sensitivum (BS) a potential antibacterial herbal drug. Scanning electron microscopy revealed the smooth fibrous morphology of the PCL membrane, whereas the drug‐loaded PCL formed fibers with more interconnective junctions with an average fiber diameter between 1 and 3 μm. Physical characterization of the membrane revealed that it has excellent mechanical stability, water vapor transmission rate and that it promotes water uptake. The release characteristics by total immersion method in phosphate buffer and acetate buffer displayed an increase in drug release with time. Finally, the antibacterial activity of the membrane was tested against standard strains of Staphylococcus aureus and Escherichia coli. PCL membranes loaded with the drug extracts were able to inhibit the growth of bacterial strains which indicated that this fibro‐porous membrane could act as a potential wound‐dressing material to treat various wounds. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013     

Combined effects of 3D bone marrow stem cell-seeded wet-electrospun poly lactic acid scaffolds on full-thickness skin wound healing

Tissue engineering has emerged as an alternative treatment to traditional grafts for skin wound healing. Three-dimensional nanofibers have been used extensively for this purpose due to their excellent biomedical-related properties. In this study, high porous 3D poly lactic acid nanofibrous scaffolds (PLA-S) were prepared by wet-electrospinning technique and seeded with rat bone-marrow stem cells (BMSCs) to characterize the biocompatibility and therapeutic efficacy of these fibers on the treating full-thickness dermal wounds. The results of in vitro andin vivo studies indicate that the 3D fibrous PLA-S can be a potential wound dressing for wound repair, particularly when seeded with BMSCs.