Effect of sodium,potassium, calcium and magnesium chloride salts on porcine muscle proteases

This study is focused on the effect of sodium chloride alternative salts (KCl, MgCl₂ and CaCl₂) on porcine muscle proteases (cathepsins, dipeptidylpeptidases and aminopeptidases). In general, KCl exerted a very similar effect to NaCl for all the studied enzymes, while the effect of divalent salts (CaCl₂ and MgCl₂) was more pronounced. Cathepsins, dipeptidyl peptidase III, dipeptidyl peptidase IV and alanyl aminopeptidase activities were strongly inhibited by all the chloride salts especially by divalent ones. Dipeptidyl peptidase II and leucyl aminopeptidase were little affected and methionyl aminopeptidase was only inhibited by divalent salts. Dipeptidyl peptidase I was strongly activated by low concentrations of the chloride salts except NaCl. Arginyl aminopeptidase was activated by NaCl and KCl and low amounts of MgCl₂, while CaCl₂ showed a strong inhibitory effect. This is very important as these enzymes play important roles in dry-cured meats and their activity is, in general, regulated by sodium chloride. Thus, reductions in the sodium concentration with subsequent increases of other alternative cations may have relevant consequences on enzyme activity that should be taken into account when processing dry-cured meats.     

Reduced‐sodium cheeses: Implications of reducing sodium chloride on cheese quality and safety

Sodium chloride (NaCl) universally well‐known as table salt is an ancient food additive, which is broadly used to increase the storage stability and the palatability of foods. Though, in recent decades, use of table salt in foods is a major concern among the health agencies of the world owing to ill effects of sodium (Na) that are mostly linked to hypertension and cardiovascular diseases. As a result, food scientists are working to decrease the sodium content in food either by decreasing the rate of NaCl addition or by partial or full replacement of NaCl with other suitable salts like potassium chloride (KCl), calcium chloride (CaCl₂), or magnesium chloride (MgCl₂). However, in cheese, salt reduction is difficult to accomplish owing to its multifaceted role in cheese making. Considering the significant contribution in dietary salt intake (DSI) from cheese, researchers across the globe are exploring various technical interventions to develop reduced‐sodium cheeses (RSCs) without jeopardizing the quality and safety of cheeses. Thus, the purpose of this study is to provide an insight of NaCl reduction on sensory, physicochemical, and technofunctional attributes of RSCs with an aim to explore various strategies for salt reduction without affecting the cheese quality and safety. The relationship between salt reduction and survival of pathogenic and spoilage‐causing microorganisms and growth of RSCs microflora is also discussed. Based on the understanding of conceptual and applied information on the complex changes that occur in the development of RSCs, the quality and safety of RSCs can be accomplished effectively in order to reduce the DSI from cheese.     

Influence of sodium replacement on the salting kinetics of pork loin

The partial replacement of sodium chloride by other salts has been proposed as a possible strategy to reduce the sodium content of cured meat products. Nevertheless, there is very little knowledge as regards the influence of salts other than sodium chloride on salting kinetics.The aim of this study was to evaluate the effect brought about by substituting sodium chloride with potassium, calcium and magnesium chloride on the salting kinetics of dry cured loins. The results showed very important changes in the salting pattern depending on the type of salts. Partial substitution of sodium affected both water and ion transport during the salting process. The presence of KCl decreased water loss while CaCl₂ and MgCl₂ had the opposite effect. However, replacement of up to 50% NaCl by KCl had no significant effect on the salting kinetics in the case of the control formulation. Calcium and magnesium cations penetrated with difficulty into the muscle remaining in the brine formed during the pile salting process. Moreover, the presence of calcium and magnesium cations considerably reduced the sodium and potassium content of the salted loin.     

Characterisation of pile salting with sodium replaced mixtures of salts in dry-cured loin manufacture

Salting, one of the fundamental operations in Spanish cured loin manufacturing, is essential to preserve the product throughout its processing and storage. However, elevated blood pressure has been identified to be the major adverse effect associated with increased sodium intake. The partial replacement of NaCl with other chloride salts such as KCl, CaCl₂ and MgCl₂ has been proposed as a possible strategy to reduce the sodium content of these products. The aim of this study was to determine the salting time needed to reach the same chloride content as commercial dry-cured loins and, to characterise the first step in dry-cured loin production (salting), in loins salted with sodium partial replaced salts. The results indicated a reduction in the salting time when using KCl as NaCl substitute and an increase in the salting time when adding CaCl₂ and MgCl₂ to the mixture. Further studies need to be done in order to fully understand the influence of these low sodium mixtures of salts on the following manufacturing steps and the final quality characteristics of the product.     

Biochemical and sensory changes in dry-cured ham salted with partial replacements of NaCl by other chloride salts

The reduction of the content of sodium chloride in dry-cured ham was studied in to prevent the problems related to high sodium intake (i.e. the hypertension). One of the possibilities to reduce the sodium content is the partial replacement of sodium chloride by mixtures of potassium, magnesium and calcium chloride salts. The effect of two salting formulations (formulation II: 50% NaCl-50% KCl and formulation III: 55% NaCl, 25% KCl, 15 CaCl₂ and 5 MgCl₂) on the protease activity through the dry-curing process and on the sensory characteristics of the final product was evaluated and compared to those of control hams (formulation I, 100% NaCl). Sensory attributes were all affected in the hams containing CaCl₂ and MgCl₂ while hams containing 50% KCl and NaCl (formulation II) were better valued, except for the attribute taste probably due to the potassium contribution to bitter taste.     

Effects of Mechanically Separated Beef with Various Chloride Salts in Restructured Beef Steaks

The effects of various chloride salts (NaCl, MgCl₂, KCl or 65% NaCl + 35% KCl) in combination with mechanically separated beef (MSB at 0, 10 or 20%) on the quality of restructured steaks formulated from bullock chucks were determined. As level of MSB increased, objectionable connective tissue, flavor desirability and overall palatability decreased and off-flavors increased. The development of oxidative rancidity during frozen storage was not affected by MSB level and MSB had little effect on textural traits. NaCl and NaCl + KCl treated steaks received superior flavor and overall palatability ratings over steaks containing MgCl₂ or KCl. Oxidative rancidity developed faster in steaks containing NaCl than the other salt types. Partial replacement (35%) of NaCl with KCl resulted in restructured steaks with sensory properties equal to and storage life superior to steaks with NaCl alone.     

Determining salt concentrations for equivalent water activity in reduced-sodium cheese by use of a model system

The range of sodium chloride (salt)-to-moisture ratio is critical in producing high-quality cheese products. The salt-to-moisture ratio has numerous effects on cheese quality, including controlling water activity (a_w). Therefore, when attempting to decrease the sodium content of natural cheese it is important to calculate the amount of replacement salts necessary to create the same a_w as the full-sodium target (when using the same cheese making procedure). Most attempts to decrease sodium using replacement salts have used concentrations too low to create the equivalent a_w due to the differences in the molecular weight of the replacers compared with salt. This could be because of the desire to minimize off-flavors inherent in the replacement salts, but it complicates the ability to conclude that the replacement salts are the cause of off-flavors such as bitter. The objective of this study was to develop a model system that could be used to measure a_w directly, without manufacturing cheese, to allow cheese makers to determine the salt and salt replacer concentrations needed to achieve the equivalent a_w for their existing full-sodium control formulas. All-purpose flour, salt, and salt replacers (potassium chloride, modified potassium chloride, magnesium chloride, and calcium chloride) were blended with butter and water at concentrations that approximated the solids, fat, and moisture contents of typical Cheddar cheese. Salt and salt replacers were applied to the model systems at concentrations predicted by Raoult's law. The a_w of the model samples was measured on a water activity meter, and concentrations were adjusted using Raoult's law if they differed from those of the full-sodium model. Based on the results determined using the model system, stirred-curd pilot-scale batches of reduced- and full-sodium Cheddar cheese were manufactured in duplicate. Water activity, pH, and gross composition were measured and evaluated statistically by linear mixed model. The model system method accurately determined the concentrations of salt and salt replacer necessary to achieve the same a_w as the full-sodium control in pilot-scale cheese using different replacement salts.     

Rheological and Gelation Properties of Meat Batters Prepared with Three Chloride Salts

The gelation and rheological properties of poultry meat emulsions prepared with three chloride salts (NaCl, KCl, MgCl₂) and 50/50 mixtures of the above salts at isoionic strength (IS = 0.42) were studied. The relationship between shear rate and shear stress for the different raw meat emulsions was found to be nonlinear and followed Bingham pseudoplastic behavior. The yield stress for the emulsion containing only MgCl₂ was significantly lower as compared to all the other treatments. The highest rigidity modulus values obtained during cooking were observed in the NaCl + MgCl₂ treatment followed by the NaCl + KCl, NaCl and KCl. The emulsion containing MgCl₂ only broke down at the initial stages of cooking, exhibiting the destabilizing effect of the magnesium cation.     

Salt Reduction in Vegetable Fermentation: Reality or Desire?

NaCl is a widely used chemical in food processing which affects sensory characteristics and safety; in fact, its presence is frequently essential for the proper preservation of the products. Because the intake of high contents of sodium is linked to adverse effects on human health, consumers demand foods with low-sodium content. A 1st step to reduce the use of salt would imply the proper application of this compound, reducing its levels to those technologically necessary. In addition, different chloride salts have been evaluated as replacers for NaCl, but KCl, CaCl₂, and ZnCl₂ show the most promising perspectives of use. However, prior to any food reformulation, there is a need for exhaustive research before its application at industrial level. Salt reduction may lead to an increased risk in the survival/ growth of pathogens and may also alter food flavor and cause economic losses. This review deals with the technological, microbiological, sensorial, and health aspects of the potential low-salt and salt-substituted vegetable products and how this important segment of the food industry is responding to consumer demand.     

Effect of selected ions from lyotropic series on lipid oxidation rate

Organic or inorganic salts, commonly present in foods as natural components or ingredients, can affect the hydrophobic/hydrophilic interactions among food components. In particular, modifying the physicochemical equilibrium in the media, the ionic species forming salts could affect the kinetics of chemical reactions occurring in foods. The aim of the present research was to study the influence of different ionic species from lyotropic series on the kinetics of lipid oxidation. For this purpose, salts containing antichaotropic (carbonate and acetate), neutral (Na⁺, K⁺) and chaotropic ions (Cl⁻) were added to soybean oil. Results indicate that potassium carbonate and potassium acetate present a strong antioxidant capacity, whereas no effect was detected for NaCl and KCl. The salt antioxidant activity was prevalently attributed to the antichaotropic anionic species present in the media, which could interact with hydroperoxides by virtue of their ability to form hydrogen bonds. These results appear to be of considerable interest for controlling the development of rancidity in foods.     

Extraction and reliable determination of acrylamide from thermally processed foods using ionic liquid-based ultrasound-assisted selective microextraction combined with spectrophotometry

Acrylamide (AAm) is a carcinogenic chemical that can form in thermally processed foods by the Maillard reaction of glucose with asparagine. AAm can easily be formed especially in frequently consumed chips and cereal-based foods depending on processing conditions. Considering these properties of AAm, a new, simple and green method is proposed for the extraction of AAm from thermally processed food samples. In this study, an ionic liquid (1-butyl-3-methylimidazolium tetrafluoroborate, [Bmim][BF₄]) as extractant was used in the presence of a cationic phenazine group dye, 3,7-diamino-5-phenylphenazinium chloride (PSH⁺, phenosafranine) at pH 7.5 for the extraction of AAm as an ion-pair complex from selected samples. Under optimum conditions, the analytical features obtained for the proposed method were as follows; linear working range, the limits of detection (LOD, 3S_b/m) and quantification (LOQ, 10S_b/m), preconcentration factor, sensitivity enhancement factor, sample volume and recovery% were 2.2–350 µg kg^?1, 0.7 µg kg^?1, 2.3 µg kg^?1, 120, 95, 60 mL and 94.1–102.7%, respectively. The validity of the method was tested by analysis of two certified reference materials (CRMs) and intra-day and inter-day precision studies. Finally, the method was successfully applied to the determination of AAm levels in thermally processed foods using the standard addition method.     

Effects of Chloride Salts on Physical, Chemical and Sensory Properties of Frankfurters

Triplicate 4.5 kg batches of frankfurters formulated with either pork/beef or mechanically deboned turkey (MDT) were made to replace all (100%) or part (35%) of the sodium chloride (NaCl) with either potassium chloride (KCl), magnesium chloride (MgCl₂) or lithium chloride (LiCl). Regardless of storage period or formulation, replacing all of the NaCl with either KCl or MgCl₂ was detrimental to flavor. This same replacement with LiCl was not detrimental to flavor in pork/beef formulations. Data suggest that MgCl₂ would not be a satisfactory substitute for NaCl in these formulations; however, replacing 35% of the NaCl with KCl may be possible. Because of its toxicity, LiCl is not GRAS-approved and we do not recommend its use but, its use in this study more closely approximated the sensory properties of NaCl than either KCl or MgCl₂.     

Effect of NaCl reduction and replacement on the growth of fungi important to the spoilage of bread

The effect of NaCl and various NaCl replacers (CaCl₂, MgCl₂, KCl and MgSO₄) on the growth of Penicillium roqueforti and Aspergillus niger was evaluated at 22 °C. In addition, challenge tests were performed on white bread to determine the consequences of NaCl reduction with or without partial replacement on the growth of P. roqueforti. From the results obtained it can be concluded that at equivalent water phase concentrations the isolates exhibited differing sensitivities to the salts evaluated with NaCl and MgCl₂ having the greatest inhibitory action on the growth of A. niger and P. roqueforti, respectively. MgSO₄ had the least antifungal activity. At equivalent molalities, CaCl₂ had in general the largest antifungal activity. Although the water activity (a_w) lowering effects of the compounds studied play a large role in explaining the trends observed, at equivalent water phase concentrations MgCl₂ was found to have a smaller inhibitory effect on A. niger than that expected from its a_w depressing effect. The challenge tests revealed that no difference occurred in the growth of P. roqueforti on standard white bread, bread with 30% less NaCl and bread in which 30% of the NaCl has been partially replaced by a mixture of KCl and Sub4Salt. These results are of importance in assessing the possible microbiological consequences of NaCl reduction or replacement in bread and similar bakery products.     

Use of a D-optimal mixture design to estimate the effects of diverse chloride salts on the growth parameters of Lactobacillus pentosus

The effects of NaCl, KCl, CaCl₂, and MgCl₂ and their mixtures on the ionic strength (IS) of the medium and the growth parameters of Lactobacillus pentosus were studied by means of a D-optimal mixture experimental design with constrains (total salt concentration ≤ 9.0%, wt/vol) and the generalized z-value. The IS was linearly related to the concentrations of the diverse salts and its increase, for similar concentrations of salts, followed the order MgCl₂ > CaCl₂ > NaCl > KCl. Within the experimental region, the lag phase duration (λ) was mainly affected by NaCl and CaCl₂ and the interaction KCl with MgCl₂. The maximum specific growth rate (μ_max) decreased as NaCl (the highest effect), CaCl₂, and MgCl₂ increased (regardless of the presence or not of previous NaCl); low KCl concentrations had a stimulating effect on μ_max, but its overall effect showed a similar trend to the other salts. The maximum population reached (N_max) was the least affected parameter and decreased as NaCl and CaCl₂ concentrations increased regardless of the presence of the other salts. The equations that expressed the growth parameters as a function of the diverse chloride salts, within the limits assayed, were developed and the corresponding z- and harmonic Z-values were estimated.     

Salmonella typhimurium Attached to Chicken Skin Reduced Using Electrical Stimulation and Inorganic Salts

Chicken skins inoculated with Salmonella typhimurium at 1 × 10⁸ CFU/mL were subjected to chemical or electrical treatments (4 mA/ cm² current, 1 kHz frequency and 50% duty cycle) for 10 min in 1% sodium chloride (NaCl), sodium carbonate (Na₂CO₃), or trisodium phosphate (Na₃PO₄ 12H₂O or TSP). Salmonellae on chicken skins and in treatment solutions and rinsing water were enumerated with microbiological platings. Chicken skin was also examined using scanning electron microscopy.S. typhimurium attached to skins were reduced by 90% after electrical treatments in 1% NaCl, Na₂CO₃, or TSP, while the reduction ranged from 34% to 76% in groups treated by the salts alone.     

27—A STUDY OF FLAME-RESIST TREATMENTS OF WOOL BY INORGANIC TIN CHEMICALS

An investigation is reported into the mode of action of two commercial flame-resist treatments for wool containing inorganic tin salts. For stannic chloride–ammonium bifluoride formulations, it is shown by ^119mSn Mössbauer spectroscopy that the tin species initially exhausted onto the fabric (SnF₅OH^2?) is chemically different from that observed after washing (SnO₂). A similar study of the potassium hexafluorozirconate–stannous chloride–hydrochloric acid system indicated that SnO₂ was formed, even before washing, and that no tin (II) species remained. The flame-resist properties of several other tin (IV) salts on wool are also reported. Stannic oxide itself appears to be ineffective as a flame-retardant for wool.     

Lipid oxidation and color change of salted pork patties

The purposes of this study were to explore the interaction of lipid oxidation and pigment oxidation in salted pork patties and to study the mutual relationship between lipid and pigment oxidation caused by replacing sodium chloride with potassium chloride at low salt levels. For fresh pork patties, a^∗ and b^∗ values decreased with the replacement of sodium chloride by potassium chloride. However, there were no significant differences of L^∗ values, pH values, total pigments and heme irons by replacement of NaCl with KCl; however, the replacement reduced 2-thiobarbituric acid reactive substances (TBARS).     

Water sorption and time-dependent crystallization behaviour of freeze-dried lactose-salt mixtures

Water sorption properties of freeze-dried lactose, lactose/CaCl₂, lactose/NaCl, lactose/MgCl₂, and lactose/KCl mixtures in their molar ratio of (9:1) were investigated. Brunauer-Emmett-Teller (BET) and Guggenheim-Anderson-de Boer (GAB) models were used to model water sorption properties. Water is known to function as a plasticizer, depressing the glass transition and facilitating crystallization. Crystallization in the present study resulted in loss of sorbed water from lactose. The crystallization of pure lactose and lactose/salt mixtures was observed at RVP?44.0% within 24 h. At RVP?54.4% water contents were higher in lactose/CaCl₂ and lactose/MgCl₂ mixtures than in pure lactose, lactose/NaCl, and lactose/KCl.Water content in pure lactose after crystallization was ?5.0%, suggesting that lactose crystallized as a mixture of α-lactose monohydrate and various anhydrous forms of α/β-lactose crystals. Anhydrous lactose/CaCl₂ and lactose/MgCl₂ had higher glass transition temperatures than lactose, but other salts (NaCl and KCl) with lactose gave lower glass transition than amorphous lactose. It seems that bivalent salts in mixtures with lactose gave a higher T_g than smaller monovalent ions. Salts delayed lactose crystallization. The effect on lactose crystallization was highest with calcium chloride (CaCl₂) and lowest with potassium chloride (KCl). It seems that different salts interacted with lactose to different extents. For water sorption, GAB model gave a better fit than BET model. Water sorption and time-dependent crystallization properties of lactose/salt mixtures should be considered in manufacturing and storage of dairy-based dehydrated materials.     

Influence of Sodium, Potassium and Magnesium Chloride on Thermal Properties of Beef Muscle

Sodium, potassium and magnesium chloride, at 1.25, 2.50 and 5.00%, were studied using differential scanning calorimetry. Increasing NaCl resulted in decrease in myosin transition temperature (T₁, first transition) and enthalpy (H₁); both showed significant (P<0.05) linear response. The sarcoplasmic proteins and collagen denaturation temperature (T₂, second transition) increased significantly over the control when 1.25% NaCl was added, but were the same as control when 2.5% and 5.0% were used. Actin denaturation temperature (T₃, third transition) tended to increase when NaCl was added, but enthalpy decreased. Potassium chloride showed similar effects to NaCl on T₁, T₂, H₁ and H₃. Increasing magnesium chloride had much more pronounced effect on decreasing second enthalpy compared to the monovalent salts. At 5% MgCl₂ T₁ increased significantly.     

EQUILIBRIUM DATA FOR OSMOTIC CONCENTRATION of POTATO IN NACL-WATER SOLUTION

Equilibrium data needed in modeling of osmotic dehydration are reported for unblanched diced potato in contact with aqueous solutions of sodium chloride. Diced potato, 1 cm cubes, were equilibrated with 0–12% (w/w) aqueous NaCl solutions at three temperatures (8, 21 and 35°C). the mass fraction of total solids nonsalt (w^F₃) in potato at equilibrium was modeled as a function of mass fraction of total solids in the fresh material at full turgor (w^F₃), concentration of NaCl in the external solution (w_IB) and temperature. Using w^F₃ and w_IB, and assuming that the activity of NaCl in the potato tissue and the external solution at equilibrium are equal, equilibrium densities were calculated based on the principle of volume additivity. the predicted and experimentally measured equilibrium densities were in close agreement.