Speckle decorrelation due to two-dimensional flow gradients

The performance of ultrasonic velocity estimation methods is degraded by speckle decorrelation, the change in received echoes over time. Because ultrasonic speckle is formed by the complex sum of echoes from subresolution scatterers, it is sensitive to the relative motion of those scatterers. Velocity gradients in flowing blood result in relative scatterer motion and can be a significant source of speckle decorrelation. Computer simulations were performed to evaluate speckle decorrelation due to two-dimensional flow gradients. Results indicate that decorrelation due to flow gradients is sensitive to the angle of flow and has a maximum at a beam-vessel angle of 0 degrees , i.e., purely axial flow. A quantitative summary of the major factors causing speckle decorrelation indicates that flow gradients are the most significant contributors under the conditions modeled.     

Improving IVUS palpography by incorporation of motion compensation based on block matching and optical flow

Intravascular ultrasound (IVUS) strain imaging of the luminal layer in coronary arteries, coined as IVUS palpography, utilizes conventional radio frequency (RF) signals acquired at 2 different levels of a compressional load. The signals are cross-correlated to obtain the microscopic tissue displacements, which can be directly translated into local strain of the vessel wall. However, (apparent) tissue motion and nonuniform deformation of the vessel wall, due to catheter wiggling, reduce signal correlation and result in invalid strain estimates. Implications of probe motion were studied on the tissue-mimicking phantom. The measured circumferential tissue displacement and level of the speckle decorrelation amounted to 12° and 0.58, respectively, for the catheter displacement of 456 μm. To compensate for the motion artifacts in IVUS palpography, a novel method based on the feature-based scale-space optical flow (OF), and classical block matching (BM) algorithm, were employed. The computed OF vector and BM displacement fields quantify the amount of local tissue misalignment in consecutive frames. Subsequently, the extracted circumferential displacements are used to realign the signals before strain computation. Motion compensation reduces the RF signal decorrelation and increases the number of valid strain estimates. The advantage of applying the motion correction in IVUS palpography was demonstrated in a midscale validation study on 14 in vivo pullbacks. Both methods substantially increase the number of valid strain estimates in the partial and compounded palpograms. Mean relative improvement in the number of valid strain estimates with motion compensation in comparison to one without motion compensation amounts to 28% and 14%, respectively. Implementation of motion compensation methods boosts the diagnostic value of IVUS palpography.     

Optical in-plane strain field sensor

A whole-field speckle strain sensor is presented. The speckle strain sensor allows the measurement of all three in-plane components of the strain field simultaneously without touching the surface of the sample. The strain fields are extracted from the in-plane motion of defocused laser speckles in a telecentric imaging system. To distinguish the contribution to the speckle motion from surface translation, rotation, and strain, the speckle motion from three lasers with different illumination directions and wavelengths has to be analyzed separately. Simultaneous acquisition of the three individual speckle patterns is achieved by means of splitting the light from the lasers onto separate but synchronized detectors with the aid of dichroic mirrors. The motion of the speckles is calculated with digital speckle photography (speckle correlation), which enables the strain sensor to measure strain fields with noise levels as low as 10 microstrain.     

Three-dimensional deformation field measurement with digital speckle correlation

Digital speckle correlation is based on a detailed analysis of changes in speckle images that are recorded from laser-illuminated rough surfaces. The two in-plane components are obtained by cross-correlation of corresponding subimages, a method also known as digital speckle photography. The local gradient of the hitherto inaccessible out-of-plane component is determined from the characteristic dependence of the speckle correlation on the spatial frequency. A detailed experimental study is carried out to analyze the new technique for systematic and random measuring errors. For moderate decorrelation the accuracy of the out-of-plane measurement is better than lambda/10 and thus comparable with interferometric techniques. Yet the extremely simple and robust optical setup is suited to nondestructive-testing applications in harsh environments. The quality of the deformation maps is demonstrated in a practical application.     

Improved speckle projection profilometry for out-of-plane shape measurement

An improved speckle projection profilometry that combines the projection of computer generated random speckle patterns using an ordinary LCD projector and the two-dimensional digital image correlation technique for in-plane displacements measurement is proposed for accurate out-of-plane shape and displacement measurements. The improved technique employs a simple yet effective calibration technique to determine the linear relationship between the out-of-plane height and the measured in-plane displacements. In addition, the iterative spatial domain cross-correlation algorithm, i.e., the improved Newton-Raphson algorithm using the zero-normalized sum of squared differences correlation criterion and the second-order shape function was employed in image correlation analysis for in-plane displacement determination of the projected speckle patterns, which provides more reliable and accurate matching with a higher correlation coefficient. Experimental results of both a regular cylinder and a human hand demonstrate that the proposed technique is easy to implement and can be applied to a practical out-of-plane shape and displacement measurement with high accuracy.     

Reduced peak-hopping artifacts in ultrasonic strain estimation using the Viterbi algorithm

Internal strain resulting from tissue deformation can be estimated by correlation processing of speckle patterns within complex (i.e., radio frequency) ultrasound images acquired during deformation. At large deformations, the magnitude of the correlation coefficient peak can be significantly lower than unity, so that random speckle correlations will exceed the true peak. This effect is called ?peak hopping? and causes significant errors in displacement and deformation estimates. Here we investigate the Viterbi algorithm, a dynamic programming procedure, to overcome peak-hopping artifacts by finding the most likely sequence of hidden states in a sequence of observed events. It is well suited to motion estimation in elasticity- imaging studies because adjacent tissue elements remain adjacent following deformation. Particularly, tissue elements along an ultrasonic beam in one image lie along a 3-D continuous curve in the next image instant. The observed event in this case is the correlation coefficient of a pixel at a certain displacement. Radio-frequency data were generated before and after deformation with an average strain of 6%. Simulations were performed for a homogenous medium and for a medium with a stiffer inclusion. Results show that Viterbi processing of speckle-tracking outputs can significantly reduce peak-hopping artifacts.     

Optimization of fringe pattern calculation with direct correlations in speckle interferometry

A direct correlation technique is used to calculate correlation fringe patterns from consecutive speckle patterns acquired with a dual-beam electronic speckle interferometer. Although more calculations are required than in standard image differencing routines, an advantage of the method is that the illumination over the surface of the object need not be uniform. In the method, Pearson's coefficient of correlation between the intensities within a set of adjacent pixels is calculated. This has the added advantage of being directly related to the theoretical phase-dependent correlation. A mapping of this measure of correlation results in the correlation fringe pattern. Laboratory tests were carried out with in-plane translations ranging from 5 to 45 mum. The correlation calculations were carried out by using cells (square sets of pixels) in the raw speckle images with dimensions ranging from 2 pixels x 2 pixels to 19 pixels x 19 pixels. Both cell dimension and translation magnitude dependent decorrelation effects influence the quality of the correlation fringe patterns.     

Uneven intensity change correction of speckle images using morphological Top-Hat transform in digital image correlation

By comparing two digital speckle images recorded before and after deformation, two-dimensional digital image correlation (DIC) method can accurately determine the in-plane displacement fields and strain fields. In a practical measurement, however, the variance of light source intensity, location and direction will cause the random uneven intensity change of the random speckle images and will lead to the obvious measurement error. Numerical simulation experiment is first carried out to analyse the influence of the recorded speckle images undergoing uneven light variation on DIC measurement accuracy. Then, a correction method for speckle images with uneven intensity change is proposed based on morphological Top-Hat transform. In addition, quantitative measurements of both in-plane rotation of a rigid body and three-point bending beam are investigated experimentally by DIC to verify the feasibility of the correction method. Experimental results show that the measurement accuracy of DIC is improved dramatically after the procedure of uneven light variation correction.     

Signal-to-noise based local decorrelation compensation for speckle interferometry applications

Speckle-based interferometric techniques allow assessing the whole-field deformation induced on a specimen due to the application of load. These high sensitivity optical techniques yield fringe images generated by subtracting speckle patterns captured while the specimen undergoes deformation. The quality of the fringes, and in turn the accuracy of the deformation measurements, strongly depends on the speckle correlation. Specimen rigid body motion leads to speckle decorrelation that, in general, cannot be effectively counteracted by applying a global translation to the involved speckle patterns. In this paper, we propose a recorrelation procedure based on the application of locally evaluated translations. The proposed procedure implies dividing the field into several regions, applying a local translation, and calculating, in every region, the signal-to-noise ratio (SNR). Since the latter is a correlation indicator (the noise increases with the decorrelation) we argue that the proper translation is that which maximizes the locally evaluated SNR. The search of the proper local translations is, of course, an interactive process that can be facilitated by using a SNR optimization algorithm. The performance of the proposed recorrelation procedure was tested on two examples. First, the SNR optimization algorithm was applied to fringe images obtained by subtracting simulated speckle patterns. Next, it was applied to fringe images obtained by using a shearography optical setup from a specimen subjected to mechanical deformation. Our results show that the proposed SNR optimization method can significantly improve the reliability of measurements performed by using speckle-based techniques.     

Monitoring of tissue thermal modification with a bundle-based full-field speckle analyzer

Speckle-contrast monitoring of laser-mediated tissue modification is examined for the specific case of delivery of speckle-modulated light from the tissue to detector (CCD camera) with a fiber-optic element (bundle). The influence of the transfer properties of a bundle-based optical system on the decorrelation rate of detected dynamic speckles is analyzed. Compared with the widely used method on the base of speckle-contrast analysis in the image plane, the considered technique is characterized by a more pronounced correlation between variations of the contrast of time-averaged speckle patterns and changes in the temperature of the modified tissue. The possibility of characterization of the modification kinetics (in particular, by the evaluation of the characteristic activation energy) using the developed speckle technique is demonstrated.     

Accuracy in electronic speckle photography

Electronic speckle photography is an accurate, easy-to-use, video-based technique for the analysis of two- and three-dimensional deformation fields and in-plane strain fields, based on numerical cross correlation. Through the use of statistical optics, simulated speckle patterns, and experiments the accuracy in electronic speckle photography was found to depend on correlation, speckle size, window size, and correlation filter. The estimated correlation was found to be the combined effect of three mutually competing factors because of classical speckle correlation, subimage overlap, and displacement gradients. In many applications white-light speckle patterns provide a more accurate estimate of the displacement field than do laser speckle patterns.     

Decorrelation-induced phase errors in phase-shifting speckle interferometry

The purpose of this research is the quantitative investigation of decorrelation-induced phase errors in speckle interferometry. Measurements in speckle interferometry are inherently affected by decorrelation, i.e., by alterations of the speckle fields during measurement. Likewise, the random phases carrying the interferometric information change during decorrelation. Image plane and pupil plane decorrelation are considered for both smooth and speckle reference wave interferometers. Since the decorrelation effect depends on the aperture and the pixel size, the calculations include not only the case of speckles being well resolved by the camera but also the case of unresolved speckles. Different standard deviations of the phase error are obtained from the probability density of the pixel modulation and the phase before and after decorrelation. Most cases (apart from pupil plane decorrelation in speckle reference wave setups) appear to obey exactly the same phase error statistics. In particular, the number of speckles per pixel does not affect the phase error distribution over the whole image. The only important parameters determining the decorrelation-induced phase errors are the amount of decorrelation and the pixel modulation.     

Real-time heterodyne speckle pattern interferometry using the correlation image sensor

A real-time method for heterodyne speckle pattern interferometry using the correlation image sensor (CIS) is proposed. The CIS demodulates the interference phase of heterodyned speckle wavefronts pixelwise at an ordinary video frame rate. The proposed method neither suffers loss of spatial resolution nor requires a high frame rate. Interferometers for out-of-plane and in-plane deformation are developed with a 200 × 200 pixel CIS camera. Experimental results confirm that the proposed method realizes real-time imaging of a rough-surfaced object under deformation. The average standard deviations of demodulated phase-difference images for the out-of-plane and in-plane interferometers are 0.33 and 0.13 rad, respectively.     

Analysis of coherent symmetrical illumination for electronic speckle pattern shearing interferometry

In an effort to find a non-contact technique capable of providing measurements of in-plane strain, a speckle shearing interferometer was designed using symmetrical coherent illumination. This paper presents an analysis of the sensitivity to displacement and strain of this interferometer, together with an analysis of the phase-stepping of the resultant fringe patterns. New notation is introduced alongside this analysis to define the interference components in speckle shearing interferometers using multiple illumination beams. Experimental results show fringe patterns and phase stepping in support of the theoretical analysis.     

Determination of Elastic Properties of Resected Human Breast Tissue Samples Using Optical Coherence Tomographic Elastography

Abstract: The present study is concerned with the application of optical coherence tomographic (OCT) elastography technique for quantitative assessment of the elastic properties of resected human breast tissue samples subjected to axial compressive loading in vitro. Three classes of breast tissue samples, namely normal, benign (fibroadenoma) and malignant (invasive ductal carcinoma), were considered. A speckle tracking technique based on two‐dimensional cross correlation was employed to track the speckle motion between original (pre‐compressed) and the displaced (post‐compressed) OCT images of the tissue samples for the measurement of displacement and strain maps. The overall data reduction approach for quantitative assessment of elastic properties was validated against the results of gelatin phantoms containing activated charcoal particles as scattering centres. Results are presented in the form of OCT images and displacement and axial strain maps for normal, benign and malignant breast tissue samples. Based on the stress–strain relationship obtained for these three classes, the values of stiffness coefficients were reported in terms of modulus of elasticity. Results of the study reveal significant differences between the two‐dimensional displacement vector maps of normal and cancerous breast tissue samples. The stiffness of benign tissue samples is found to be about two times higher than that of normal tissue samples, whereas for malignant samples, it is about four times higher, thereby signifying appreciable differences in the stiffness of cancerous and normal tissue samples.     

Nonscanning measurements for determining in-plane mode shapes in piezoelectric devices with polished surfaces

A nonmechanical scanning method has been developed for the visualization of the in-plane mode shapes of piezoelectric devices with polished surfaces. By taking into account the reflection versus laser-wavelength characteristics of the material of the electrodes, the in-plane motion can be measured even if the surface of the measurement plane is polished like a mirror. This method is based on laser speckle interference and two-dimensional correlation filtering that effectively enhance the mode-shape visualization for bulk and surface acoustic wave devices. Although this method cannot directly measure absolute displacement, the simple measurement system and high speed measurement more than offset this disadvantage. The experimental results for fundamental thickness-shear and nearby inharmonic modes in a bimesa-shaped rectangular AT-cut quartz resonator have been presented. The results of the experiments and the analyses obtained by the three-dimensional finite element analyses correlate well and show the advantages and validity of the proposed technique.     

Systematic and random errors in electronic speckle photography

Electronic speckle photography offers a simple and fast technique for measuring in-plane displacement fields in solid and fluid mechanics. Errors from undersampling, illumination divergence, and displacement magnitude have been analyzed and measured. The nature of the systematic error is such that a drift toward the closest integral pixel value is introduced. Because of the finite extent of the sensor area, considerable undersampling is tolerable before systematic errors occur. The random errors are mainly dependent on the effective ?-number of the imaging system and speckle decorrelation introduced by object displacement. When sampling at a rate of ~ 70% of the Nyquist frequency, we avoided systematic errors and minimized random errors.     

Internal displacement and strain imaging using ultrasonic speckletracking

Previous ultrasound speckle tracking methods have been extended, permitting measurement of internal displacement and strain fields over a wide dynamic range of tissue motion. The markedly increased dynamic range of this approach should lead to enhanced contrast resolution in strain and elasticity images. Results of experiments on gelatin-based, tissue equivalent phantoms show the capabilities of the method     

Ultrasound three-dimensional velocity measurements by featuretracking

This article describes a new angle-independent method suitable for three-dimensional (3-D) blood flow velocity measurement that tracks features of the ultrasonic speckle produced by a pulse echo system. In this method, a feature is identified and followed over time to detect motion. Other blood flow velocity measurement methods typically estimate velocity using one- (1-D) or two-dimensional (2-D) spatial and time information. Speckle decorrelation due to motion in the elevation dimension may hinder this estimate of the true 3-D blood flow velocity vector. Feature tracking is a 3-D method with the ability to measure the true blood velocity vector rather than a projection onto a line or plane. Off-line experiments using a tissue phantom and a real-time volumetric ultrasound imaging system have shown that the local maximum detected value of the speckle signal may be identified and tracked for measuring velocities typical of human blood flow. The limitations of feature tracking, including the uncertainty of the peak location and the duration of the local maxima are discussed. An analysis of the expected error using this method is given