Clocks regulating developmental processes

The goal of this study was to investigate the role of chronic otitis media with effusion as a cause of childhood imbalance. Nineteen 4- to 6-year-old children with chronic otitis media with effusion and 14 matched control children underwent a series of laboratory tests, including two standardized tests of balance and locomotion: the Peabody Developmental Motor Scales (PDMS) and the Bruininks-Oseretsky Test of Motor Proficiency (BOTMP). The children in the chronic otitis media with effusion group underwent tympanostomy tube insertion. Both groups underwent the same battery of tests within 6 to 8 weeks of initial testing. Analysis of variance for the PDMS and BOTMP demonstrated significantly lower performance in the balance subscales for the otitis group before (P < .01). Both otitis and control groups improved when retested. The improvement was greater and approaching significance in the otitis group on the PDMS and BOTMP (P = .056 and .097, respectively). It is concluded that chronic otitis media with effusion significantly affects balance and coordination skills in 4- to 6-year-old children. These skills improve after tympanostomy tube insertion at a rate greater than that of the control group.     

PTHrP regulates epidermal differentiation in adult mice

BACKGROUND AND PURPOSE: A new gait training strategy for patients with stroke proposes to support a percentage of the patient's body weight while retraining gait on a treadmill. This research project intended to compare the effects of gait training with body weight support (BWS) and with no body weight support (no-BWS) on clinical outcome measures for patients with stroke. METHODS: One hundred subjects with stroke were randomized to receive one of two treatments while walking on a treadmill: 50 subjects were trained to walk with up to 40% of their body weight supported by a BWS system with overhead harness (BWS group), and the other 50 subjects were trained to walk bearing full weight on their lower extremities (no-BWS group). Treatment outcomes were assessed on the basis of functional balance, motor recovery, overground walking speed, and overground walking endurance. RESULTS: After a 6-week training period, the BWS group scored significantly higher than the no-BWS group for functional balance (P = 0.001), motor recovery (P = 0.001), overground walking speed (P = 0.029), and overground w alking endurance (P = 0.018). The follow-up evaluation, 3 months after training, revealed that the BWS group continues to have significantly higher scores for overground walking speed (P = 0.006) and motor recovery (P = 0.039). CONCLUSIONS: Retraining gait in patients with stroke while a percentage of their body weight was supported resulted in better walking abilities than gait training while the patients were bearing their full weight. This novel gait training strategy provides a dynamic and integrative approach for the treatment of gait dysfunction after stroke.     

Detection and determination of the major metabolites of [3H]cytosine arabinoside by high-performance liquid chromatography

On sudden presentation of a danger stimulus, the crab Chasmagnathus elicits an escape response that habituates promptly and for a long period. We have previously reported that administration of a cAMP-permeable analog (CPT-cAMP) along with a phosphodiesterase inhibitor (IBMX) improves long-term habituation (LTH). In present experiments we studied the effect of systemic administration of the protein kinase A (PKA) activator Sp-5,6-DCl-cBIMPS and that of the PKA inhibitor Rp-8-Cl-cAMPS on LTH tested 24 h after a weak training protocol (5 trials of danger stimulus presentation) or a strong training protocol (15-30 trials), respectively. A 50 microliters pre-training injection of 75 microM Sp-5,6-DCl-cBIMPS, and to a lesser degree of 25 microM, improved retention of the habituated response but not affect short-term habituation (STH). Like pre-training injection, post-training administration of Sp-5,6-DCl-cBIMPS proved to exert a facilitatory action on retention though with 75 microM dose only. Conversely, both pre- and post-training injection of 25 microM Rp-8-Cl-cAMPS impaired LTH without affecting STH. Thus, the PKA activator Sp-5,6-DCl-cBIMPS enables a weak training to produce LTH while the PKA inhibitor Rp-8-Cl-cAMPS impairs LTH when a strong training is given. Activation of crab PKA by Sp-5,6-DCl-cBIMPS and its inhibition by Rp-8-Cl-cAMPS were assessed using an in vitro PKA activity assay. These results provide independent evidences supporting the view that PKA plays a key role in long-term memory storage in this learning paradigm.     

Behavior consultation: A five-year evaluation of consultant and client outcomes.

Examined the effects of training in behavioral consultation (BEC) on school psychology graduate students and on client outcomes. 169 children (aged 3–11 yrs), 29 teachers/consultees, and 26 consultants participated. The consultants were comprised of 4 groups of graduate students and 1 no-training undergraduate control group. The procedure involved 3 phases of assessment: pre-training, competency-based training, and post-training. Training focused on the principles and mastery of applied behavioral therapy and BEC, relationship skills, and systems entry issues. The dependent variables included indices of mastery of the BEC process, knowledge and attitude toward behavior modification (BEM), consumer satisfaction, and treatment outcome. Each consultant achieved a predetermined minimum criterion of 80% of BEC interview objectives. Training resulted in large increases of BEM and no changes in attitude toward BEM. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Intrahippocampal GMP administration improves inhibitory avoidance performance through GABAergic and glutamatergic mechanisms in rats

Guanidino compounds play specific physiological and pathological roles in the central nervous system. We investigated the effect of an intrahippocampal infusion of GMP (a guanidino compound) administered immediately post-training on the inhibitory avoidance learning paradigm in rats. Bilateral intrahippocampal micro-injection of GMP (0-30 nmol) caused a dose-dependent increase in test step-down latencies which was completely reversed by intrahippocampal co-administration of muscimol or baclofen (GABA agonists) or preadministration (15 min pre-training, i.p.) of MK-801 (an NMDA antagonist). These results provide evidence for a participation of GABA and NMDA receptors in the GMP-induced increase in the test step-down latencies.     

Evaluation of Floor Vibration in a Biotechnology Laboratory Caused by Human Walking

A floor supported on long-span beams, which was designed to accommodate bio research instruments, is evaluated for vibration induced by people walking. First, a brief review in vibration criteria is given. The variation of force time histories imposed by people’s feet on supporting objects is also discussed. Both beam and floor finite-element models are then used to simulate the local walking response of the floor mathematically. Footfall forces are applied to the finite-element models via triangular distribution function. A comparison of the time history analysis results with the vibration criteria shows that the floor performs well under people walking. Field measurements were also conducted after the completion of the construction. The measured results show a good correlation with the finite-element analysis results. During the analyses, it was also found that as long as the local floor model covers a structural bay, the boundary conditions of the floor model do not affect the response much. Using an equivalent constant footfall force function can produce similar results compared with those obtained using a more sophisticated force function.     

In whose ''best interests''? Ethical issues involved in the moral dilemmas surrounding the removal of sexually abused adolescents from a community-based residential treatment unit to a locked, forensic adult psychiatric unit

BACKGROUND: Physiological and psychological markers of patients with congestive heart failure (CHF) who will respond to aerobic training are needed as a guide to appropriate therapy. METHODS: Seventeen of 21 patients with stable CHF completed a 16-week supervised progressive walking program 5 times per week. Cycle ergometer determinations of peak oxygen intake and peak power output at entry and 16 weeks were supplemented by a 6-minute walk, a disease-specific Quality of Life (QOL) questionnaire, and a standard gamble. RESULTS: Peak oxygen intake increased by 2.6 +/- 1.5 mL/(kgmin) over an initial value of 15.6 mL/(kgmin), with parallel gains in peak power and the 6-minute walk. Marked improvements in QOL and standard gamble scores also developed. Initial cardiorespiratory status (heart volume, ejection fraction, oxygen pulse, and peak oxygen intake) was correlated more closely (P = 0.09 to 0.18) with delta peak oxygen intake than with delta peak power or delta walking distance. Physiological gains bore little relationship to initial psychological status. Gains in CHF Questionnaire and Standard Gamble scores were strongly associated with initial scores for these variables (dyspnea, P = .02; mastery, P = .005; standard gamble, P = .001), but could not be predicted from either initial physiological status or gains in physiological condition. CONCLUSIONS: Initial cardiorespiratory status provides little indication of which patients with CHF respond well to training. Gains in QOL score are influenced by initial scores, and seem to show a "ceiling" effect.     

Selective peripheral neurotomy of the hamstring branches of the sciatic nerve in the treatment of spastic flexion of the knee. Apropos of a series of 11 patients

BACKGROUND: A series of 11 patients (9 adults and 2 children) were operated on by selective peripheral neurotomy for spastic knee in flexion. The objective of surgery was to restore bipodal stance and therefore walking for the adult patients and to improve walking for the diplegic children. METHODS: Spasticity was secondary to stroke or cranial trauma for the adult patients. The two children presented with Little disease. The delay between onset and surgery was on the average of 2 years. All the patients had a passive flexum of the knee of 20 degrees, and an excessive spasticity of the hamstrings (average Held score of 4.55). Peripheral selective neurotomies consist of a partial section of the spastic muscle's motor fascicles. The partial section concerns the afferent fibers to the spinal cord whose interruption leads to the disappearance of spasticity. The section also involves the motoneurones' axons and thus leads to partial denervation. RESULTS: The spasticity of the treated muscles disappeared in the 11 patients. Average follow-up was 16 months. The neurotomies led to improvement of walking without splint for 3 walking patients, and restored walking with a splint for 5 patients. One patient failed to walk. The two children had a dramatic improvement of their walk. CONCLUSION: Our results incite us to increasingly indicate this type of neurotomy in the treatment of the spastic lower limb.     

Does walking speed influence the time pattern of muscle activation in normal children?

Dynamic electromyography (EMG) of the extrinsic muscles of the ankle is used more and more frequently to assist in the planning of tendon transfers in children with equinovarus deformities. Since these children walk at low speeds (1 to 4km/h), and since walking speed modifies the EMG-time pattern, the clinical investigator must be able to differentiate EMG modifications due to pathology versus those due to slow walking speed. The aim of this work was to study the effect of walking speed on the EMG-time pattern of the extrinsic ankle muscles in healthy children between 4 and 11 years of age. This pattern was found to change significantly with speed of progression but is independent of growth over this age range. A nomograph of EMG timing, taking into account walking speed, is proposed for clinical gait analysis.     

The street food culture of Guatemala City: a case study from a downtown, urban park

A number of experimental studies have reported that moderate hypothermia can produce significant protection against behavioral deficits and/or morphopathological alterations following traumatic brain injury; a Phase 3 clinical trial is currently examining the therapeutic potential for moderate hypothermia (32 degrees C) to improve outcome following severe traumatic brain injury in humans. The current study examined whether hypothermia (32 degrees C) provided behavioral protection following experimental cortical impact injury. The extent of focal cortical contusion was also examined in the same rats. A total of 30 male Sprague-Dawley rats were trained on beam balance and beam walking tasks prior to injury. Under isoflurane anesthesia, cortical impact was produced on the right parietal cortex of 20 rats. Ten rats underwent all surgical procedures but were not impacted (sham-injured rats). Ten of the injured rats were cooled to 32 degrees C (measured in temporalis muscle) beginning 5 min postinjury, maintained for 2 h and rewarmed slowly for 1 h. In the other 10 injured rats, normothermic temperatures (37.5 degrees C) were maintained for the same duration. Beam balance and beam walking performance was assessed daily for 5 days following injury. At 11 days postinjury, rats were assessed for 5 days on acquisition of the Morris water maze task. Following behavioral assessments, rats were perfused and the brain removed. Coronal sections were cut through the site of cortical impact injury and stained with hematoxylin and eosin. Hypothermic treatment resulted in significantly less beam balance and beam walking deficits than observed in normothermic rats. Hypothermia also significantly attenuated spatial memory performance deficits. Quantitative morphometric analyses failed to detect any significant differences in volumes of necrotic tissue cavitation in cortices of hypothermic and normothermic rats. Hypothermic treatment also had no effect on volumes of dorsal hippocampal tissue or numbers of cells in CA1 or CA3 regions of the hippocampus. These data suggest that hypothermia, consistent with the reports of others, can produce significant behavioral protection following cortical impact injury that is not necessarily correlated with changes in focal cortical necrosis within the first 15 days following injury.     

The fen-phen finale: a study of weight loss and valvular heart disease

Exercise tolerance may be reduced in patients with cystic fibrosis, but it is not always possible to predict this from standard lung function measurements. Formal exercise testing may, therefore, be necessary, and the test should be simple and readily available. We have developed a "3-minute step test" and compared it with the standard 6-minute walking test. Subjects stepped up and down a 15-cm-high single step at a rate of 30 steps per minute for 3 minutes. The effect of the step test on spirometry was tested first in 31 children with CF (mean age, 12.0 years), who had a mean (range) baseline forced expired volume in 1 second (FEV1) of 64% (18-94%) of predicted values. The step test was then compared with the standard 6-minute walk in a further 54 patients with cystic fibrosis (mean age, 12.5 years), with mean (range) baseline FEV1 of 61% (14-103%) of predicted values. Outcome measures were minimum arterial oxygen saturation (SaO2), maximum pulse rate, and the modified Borg dyspnea score. Post-step test spirometry showed mean (95% CI) changes of -1.1% (-6.0 + 3.9%) for forced vital capacity, of -1.6% (-4.2 + 1.1%) for FEV1, and +0.25% (-2.8 + 3.3%) for peak expiratory flow, although 5/31 children showed >15% drop in one or more parameters. The step and walk tests both produced significant changes (P < 0.0001) in all outcomes, with a mean (range) minimum SaO2 of 92% (75-98%) versus 92% (75-97%), a maximum pulse rate of 145 b.p.m. (116-189) versus 132 (100-161), and a Borg score of 2.5 (0-9) versus 1.0 (0-5), respectively. Comparison of the two tests showed that the step test increased breathlessness (mean change Borg score, 2.3 vs. 0.8; P < 0.0001) and pulse rate (mean change, 38% vs. 24%, P < 0.0001) significantly more than the walk, whereas the decrease in SaO2 was similar (mean change, -2.9% vs. -2.6%; P = 0.12). Some patients with a significant drop in SaO2 (>4%) would not have the decrease predicted from their baseline lung function. Reproducibility for the two tests was similar. The step test is quick, simple and portable, and is not dependent on patient motivation. Although the step test is more tiring, its effect on SaO2 is similar to the 6-minute walking test. It is a safe test that may prove to be a valuable measure of exercise tolerance in children with pulmonary disease, although longitudinal studies are now needed.     

Letter training and its effect on the development of beginning reading skills.

The effect of training to improve letter-naming speed on beginning reading skill was evaluated with 39 1st-grade students. Ss were randomly assigned to training and control groups and were administered curriculum-based measures of reading at pretraining, post-training, and follow-up. Trained Ss demonstrated significantly faster letter-naming speed at post-training assessment as compared with untrained Ss. However, the difference in letter-naming speed did not lead to a significant difference in overall measured reading skill as evaluated by an analyses of covariance. Scores for 1 of the 4 reading measures (Fall passage oral reading rate) demonstrated a significant difference at post-training, but were not significant at follow-up. In 2 of the 3 remaining measures of beginning reading skill, differences between the mean scores of trained and untrained Ss were greatest at points where the assessment of skills most closely matched the content of classroom instruction. However, these differences were not statistically significant. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Anthropometric and physical performance characteristics of male volleyball players

Fourteen Finnish and ten Russian elite male volleyball players were studied for their anthropometric dimensions, maximal isometric trunk extension and flexion, leg extension strength and vertical jumping height. In addition, the height of rise of the body centre of gravity h (C.G.), and the height of the hand and ball were analyzed from a video tape in spike and block jumps taken during actual competition. The two teams were found to differ significantly in the h (C.G.) during a vertical jumping test where a preliminary counter movement was allowed and in the lengths of lower limbs and legs; the Russian volleyball players jumped higher and had longer lower extremities. In actual competition, the hands of the Russian players while performing a spike were on the average ten centimeters higher (p less than .01) than the hand of the Finnish players. No significant differences were found, however, between the teams in the h (C.G.) during spiking. This finding seems to suggest that the Russians have better spike technique.     

Effects of therapy on maximal walking time following femoral ligation in the rat

Maximal walking times at a constant rate of 10 m/min were compared in normal rats, normal rats that had been exercised daily for 6 weeks, and rats that had undergone bilateral femoral artery ligation followed by 6 weeks of either an oral vasodilator, absence of sympathetic impulses to the extremities, cold exposure, or daily exercise. Rats maintained on the vasodilator could walk no farther than untreated, ligated rats. Both chronic cold exposure and sympathectomy significantly increased maximal walking distances of ligated rats and daily exercise increased maximal walking distances 7-fold. Rats that had received 6 weeks of exercise training following femoral artery ligation could walk longer than normal untrained rats; therefore, the increased exercise tolerance could not be explained solely by the restoration of normal large artery conductance by enlargement of collaterals bypassing the femoral occlusion.     

Effects of maximally tolerated oral therapy on the six-minute walking test in patients with chronic congestive heart failure secondary to either ischemic or idiopathic dilated cardiomyopathy

In patients with heart failure, therapy with "maximally tolerated" oral doses of diuretics, vasodilators, and digitalis results in a significant increase in the distance walked during the 6-minute walking test, compared with conventional therapy at "standard" doses, indicating an improvement in exercise tolerance. The 6-minute walk test is a simple, inexpensive, and well-tolerated test to measure changes in exercise tolerance induced by pharmacologic interventions, even on a short-term basis.     

Apolipoprotein E antibodies affect the retention of passive avoidance memory in the chick

Isoforms of apolipoprotein E (ApoE) have been implicated as risk factors in Alzheimer's disease. We have, therefore, examined the possible role of ApoE in memory formation, using a one-trial passive avoidance task in day-old chicks. Birds were trained on the task and then at various times pre or post-training were injected intracerebrally with anti-ApoE. Immunofluorescence staining demonstrated the presence of the antibody bound to the neuropil, close to the injection site and adjacent to the ventricle, with a residence time in the brain of up to 30 min. Chicks that were injected 30 min pre-training or just post-training with 5 micrograms/hemisphere of the antibody learned the task, but were amnesic when tested at 30 min or at subsequent times up to 24 hr post-training. When tested at 24 hr, birds injected 5.5 hr post-training showed unimpaired retention. Birds injected with 5 micrograms/hemisphere of anti-ApoA-I (which has a brain distribution similar to that of anti-ApoE) at 30 min pretraining showed no amnesia, indicating the specificity of the effect to the ApoE. Possible mechanisms for this effect are discussed.     

Tuning in to another person's action capabilities: Perceiving maximal jumping-reach height from walking kinematics.

Three experiments investigated the ability to perceive the maximum height to which another actor could jump to reach an object. Experiment 1 determined the accuracy of estimates for another actor's maximal reach-with-jump height and compared these estimates to estimates of the actor's standing maximal reaching height and to estimates of the perceiver's own maximal reaching and reach-with-jump height. Perception of another actor's maximum reach-with-jump height was less accurate than the other estimates, but still accurate to within 8% error. The actor's reach-with-jump height was modified in Experiment 2 by attaching weights around the actor's ankles. Perceivers, who were explicitly aware of the manipulation, adjusted their maximum reach-with-jump estimates for the actor accordingly. In Experiment 3, perceivers were not explicitly aware of the weight manipulation, but provided significantly lower maximum reach-with-jump estimates after watching the actor walk while wearing the weights compared to estimates obtained after watching the actor walk while not wearing the weights. The results suggest that the actor's walking pattern was informative about the actor's capacity to produce a different action, jumping to reach an object. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Risk factors for diabetic foot ulceration: a logical approach to treatment

Children with limb deficiencies or amputations of the lower extremities should be enabled to stand or walk according to their state of development, because standing and walking are among the most important preconditions for the best possible integration. Supplying them with a prosthesis, orthosis and orthoprosthesis is therefore indispensable for rehabilitation. While a prosthesis replaces parts of the extremities, an orthosis stabilizes the existing extremity. Orthoprostheses compensate longitudinal malformations, have a supporting effect, allow growth to be controlled and compensate for shortening. Just as important as the remedy is early medical treatment. The medical team taking care of the patient works out a treatment plan where responsible cooperation with the parents is of utmost importance. The focus of all efforts is, of course, the handicapped child. Examples are used to demonstrate the course of successful rehabilitation of children and adolescents with damaged limbs. Equally the psychological situation of the parents and child is taken into consideration.     

Variability in energy cost and walking gait during race walking in competitive race walkers

PURPOSE: The aim of this study was to examine the variability of energy cost (Cw) and race walking gait after a 3-h walk at the competition pace in race walkers of the same performance level. METHODS: Nine competitive race walkers were studied. In the same week, after a first test of VO2max determination, each subject completed two submaximal treadmill walks (6 min length, 0% grade, 12 km X h(-1) speed) before and after a 3-h overground test completed at the individual competition speed of the race walker. During the two submaximal tests, subjects were filmed between the 2nd and the 4th min, and physiological parameters were recorded between the 4th and the 6th min. RESULTS: Results showed two trends. On the one hand, we observed a significant and systematic increase in energy cost of walking (mean deltaCw = 8.4%), whereas no variation in the gait kinematics prescribed by the rules of race walking was recorded. On the other hand, this increase in metabolic energy demand was accompanied by variations of different magnitude and direction of stride length, of the excursion of the heel and of the maximal ankle flexion at toe-off among the race walkers. CONCLUSION: These results indicated that competitive race walkers are able to maintain their walking gait with exercise duration apart from a systematic increase in energy cost. Moreover, in this form of locomotion the effect of fatigue on the gait variability seems to be an individual function of the race walk constraints and the constraints of the performer.