Effect of enhancement and ageing on flavor and volatile compounds in various beef muscles

To identify and quantify selected flavor-active volatile compounds and relate them to sensory characteristics, the gluteus medius (round), rectus femoris (round), vastus lateralis (round), vatsus medialis (round), teres major (chuck), infraspinatus (chuck), complexus (chuck), serratus ventralis (chuck), psoas major (loin) and longissimus dorsi (loin) were removed from heifer carcasses, enhanced, vacuum packaged, aged for 7 or 14days, steaks were cut, vacuum packaged and frozen (48h). Flavor-active volatiles affected by enhancement and ageing in the various muscles included nonanal, 2,3-octanedione, pentanal, 3-hydroxy-2-butanone, 2-pentyl furan, 1-octen-3-ol, butanoic acid, pentanal and hexanoic acid, compounds often associated with lipid oxidation. Enhancement decreased hexanal and hexanoic acid. Ageing decreased butanoic acid. Pentanal content varied among muscles depending on enhancement and ageing. Livery off-flavor was positively correlated with pentanal, hexanal, 3-hydroxy-2-butanone and hexanoic acid. Rancid off-flavor was correlated with pentanal and with 2-pentyl furan but not with hexanal.     

Quality changes in beef gluteus medius, infraspinatus, psoas major, rectus femoris, and teres major enhanced prior to aging

ABSTRACT:  The objective of this study was to evaluate the effects of enhancement and aging on quality characteristics of the gluteus medius (round), rectus femoris (round), teres major (chuck), infraspinatus (chuck), and psoas major (loin). Muscles were enhanced, vacuum packaged, and aged for 7 or 14 d. Aging affected enhanced compared with nonenhanced beef differently. After 7 d of aging, enhanced beef experienced more cook loss and was less red (higher hue angle, lower a * values, and chroma) than did nonenhanced beef; after 14 d of aging, these differences were lost. Enhancement increased tenderness and brown color of the gluteus medius (round); however, it decreased visual green and red colors. Enhanced gluteus medius experienced 3.1% lower purge losses, lower L * values, and chroma than their nonenhanced counterparts. Enhancement increased infraspinatus (chuck) tenderness and visual brown color, and decreased visual red and green colors, purge loss, L * value, a * value, and chroma. The enhanced infraspinatus was substantially more tender than the other muscles evaluated, other than the gluteus medius ; however, it was also substantially more visually brown. Enhancement decreased purge loss of the psoas major . Enhancement increased L * and a * values, hue angles, and chroma than other enhanced muscles. After enhancement the tenderness of the rectus femoris increased by 10%, decreased purge losses from 4.5% to 1.7%, and decreased green color by 18%. It reduced L * and a * values and chroma. Aging increased tenderness. Overall, muscles from the chuck ( infraspinatus and teres major ) appeared to benefit the most from enhancement.     

Enhancing palatability traits in beef chuck muscles

Thirty-six USDA Select complexus, latissimus dorsi, rhomboideus, serratus ventralis (SEV), splenius, subscapularis, supraspinatus (SUS), and triceps brachii (TRB) muscles were studied. Muscles were assigned to one of four treatments: control, marinated, needle-pumped, and vacuum-tumbled to reach 10% brine pick-up after treatment. The solution was formulated to have 0.5% of sodium chloride and 0.4% of sodium tripolyphosphate in the end product. Steaks from the SEV, SUS, and TRB muscles were cooked on a grill and the other five muscles were oven-roasted whole, all of them cooked to an internal temperature of 71°C. Sensory evaluations and Warner-Bratzler shear force (WBSF) determinations were conducted immediately after cooking (Day-1), and after being cooked and stored frozen for 60 days (Day-60). For the Day-60 evaluation, samples were reheated using a microwave oven. The needle-pumped treatment reduced WBSF values compared to the control in 3 out of 8 muscles (P<0.05). The marinated treatment appeared to have the greatest influence on juiciness and was different from the control in 7 of 8 muscles (P<0.05). Comparing treated muscles to the control, tenderness was increased in 4 out of 8 muscles when needle pumped (P<0.05). Brine treatment reduced sensory detected connective tissue only in 2 of 8 muscles. More off-flavors were detected by the panel for marinated samples from six of eight muscles. All muscles had lower values for WBSF, less connective tissue and off-flavors, and higher juiciness, overall tenderness, and beef flavor intensity on Day-1 than Day-60. The needle-pumped method was slightly superior to the marinated and vacuum-tumbled treatments even though the differences were not always consistent. But, regardless of the application method, palatability traits were generally enhanced by brine treatments.     

The influence of cooking rate and holding time on beef chuck and round flavor

Steaks from seven muscles from 10 beef carcasses were cooked quickly or slowly and held 0 or 1 h to explore the influence of cooking rate and holding time on beef flavor. Moisture, ash, pH, and heme–iron concentration were determined for each muscle. Trained sensory panels evaluated the steaks for tenderness, juiciness, connective tissue, and off-flavor intensity in addition to identifying specific off-flavors. Off-flavor intensity was lowest when beef was cooked slowly (on a 149 °C gas grill instead of a 249 °C grill) and when it was held for 1 h prior to sensory evaluation. The M. infraspinatus had the least intense off-flavor and the M. vastus intermedius had the most intense off-flavor. Slow cooking or holding for 1 h prior to consumption reduced the intensity of off-flavor in value cuts from the beef chuck and round while chemical characteristics did not contribute to off-flavor in this study.     

Factors influencing the variation in tenderness of seven major beef muscles from three Angus and Brahman breed crosses

Beef carcasses (n=30) from 3/4 Angus (A)×1/4 Brahman (B), 1/4A×3/4B, and 1/2A×1/2B F(1) crosses were used to evaluate breed type, electrical stimulation, and postmortem aging on the M. semimembranosus (SM), M. semitendinosus (ST), M. biceps femoris (BF), M. vastus lateralis (VL), M. gluteus medius (GM), M. longissimus dorsi lumborum (LD), and M. triceps brachii (TB). Shear force values decreased with increased postmortem aging to a greater extent in steaks from 3/4A×1/4B than steaks from the other breed types. Shear force values for steaks from the round (SM, ST, BF, VL) were higher than steaks from the loin (LD, GM) and chuck (TB) for both electrically stimulated and non-electrically stimulated muscles. In the LD muscle, calpastatin activities were similar among breed types. Muscle type played the greatest role in determining tenderness.     

Tenderizing, Ageing, and Thawing Effects on Sensory, Chemical, and Physical Properties of Beef Steaks

The effects of electrical stimulation, tender stretching, ageing, freezing and thawing on beef palatability were assessed by a trained sensory panel and by several objective methods. The overall palatability of both longissimus dorsi (strip loin) and semimembranosus (topside) steaks improved with ageing, irrespective of tenderizing method, but there appeared to be little advantage in extending ageing beyond 10 days. Tender stretched longissimus dorsi steaks were marginally preferred by the taste panel to electrically stimulated steaks subjected to the same ageing. Unfrozen steaks were preferable to frozen and thawed steaks with respect to juiciness, flavor and tenderness. Thawing method had little effect on steak palatability.     

Profile of biochemical traits influencing tenderness of muscles from the beef round

The objective of this study was to define the biochemical differences that govern tenderness and palatability of economically important muscles from the beef round using cuts with known tenderness differences. At 24h postmortem, the longissimus dorsi (LD), gracillus (GR), adductor (AD), semimembranosus (SM), sartorius (SAR), vastus lateralis (VL), and vastus intermedius (VI) muscles were removed from ten market weight beef cattle. Sensory and biochemical characteristics were determined in each cut and compared with the LD. The GR, SAR and VI had sensory traits similar to the LD while the SM, AD and VL differed. The GR, SAR, AD, and SM all had multiple biochemical characteristics similar to the LD, while the VI and AD had numerous biochemical differences. While no one biochemical characteristic can be used to predict tenderness across all muscles, analysis of the biochemical characteristics revealed that in most beef round cuts postmortem proteolysis provided a good indication of the tenderization occurring during aging.     

Palatability and Storage Characteristics of Precooked Beef Roasts

The palatability and storage characteristics of fresh, frozen and precooked beef chuck roasts prepared using added phosphate-salt and vacuum cooking bags were evaluated. Palatability attributes (tenderness, juiciness, beef flavor intensity, and off-flavor intensity) of precooked roasts prepared in vacuum cooking bags with phosphate-salt were as good or better than freshly cooked roasts. Precooked roasts in vacuum cooking bags had the lowest total bacterial counts after storage for 14 and 28 days. A palatable precooked roast beef product was developed that can be stored for 28 days at 4°C.     

Tenderness Improvement of the Major Muscles of the Beef Carcass by Electrical Stimulation

Steaks (arm; blade; seven-bone; rib, small-end; top loin; tenderloin; top sirloin; tip, cap-off; and round) from electrically stimulated (ES) and control sides of 20 beef carcasses were cut and frozen at 5 days postmortem. Forequarter muscles that were improved by ES were the longissimus dorsi (rib steak), the subscapularis and supraspinatus (blade steak), and the superficial pectoral (arm steak). Hindquarter muscles that were improved by ES were the longissimus dorsi (top loin steak), the biceps femoris and gluteus medius (top sirloin steak), and the semimembranosus and biceps femoris (round steak). ES, in addition to improving tenderness of the longissimus dorsi, increased tenderness of other major muscles in the carcass.     

Effect of Muscle Type and Mixing Time on Sectioned and Formed Beef Steaks

Sectioned and formed beef steaks were manufactured from Standard beef rounds, Choice beef plates and Choice, Yield Grade 3, Meat Buyers Guide (126) 3-piece chucks. Replicates were established by slaughter day. A trained sensory panel evaluated juiciness, flavor, tenderness, and connective tissue residue. Color scores were assigned to steaks from each treatment. Instron Kramer shear was used to evaluate tenderness and thiobarbituric acid (TBA) was used to measure rancidity. Reflectance spectrophotometry indicated oxymyoglobin and metmyoglobin content. Mixing improved tenderness (P < 0.01) by 20% after 18 min. An 8% improvement occurred after 6 min. Mixing increased sensory juiciness (P < 0.01) and flavor (P < 0.05). Subjective and objective measures indicated that steaks processed from chuck were less tender (P < 0.01). Cooking yields improved linearly (P < 0.01) due to mixing. The TBA values were low and did not change due to treatment or after 90 days storage at –30°C. Subjective and objective color analyses indicated highly desirable color for fresh beef. Steaks processed from chuck meat were higher in percent metmyoglobin.     

The effects of quality grade, aging, and location within muscle on Warner-Bratzler shear force in beef muscles of locomotion

Aging patterns of nine muscles from the chuck and round from two quality classifications of beef: USDA Select and upper 2/3 of USDA Choice grade were determined. The following muscles were evaluated: infraspinatus, triceps brachii - lateral head, triceps brachii - long head, serratus ventralis, complexus, splenius, rhomboideus, vastus lateralis and rectus femoris. Muscles were divided into four portions, progressing from anterior to posterior or dorsal to ventral orientation to the carcass depending on muscle fiber orientation. One steak was removed from each portion and aged for 7, 14, 21, or 28 d postmortem, then Warner-Bratzler shear force (WBSF) analysis was conducted. Consistent aging recommendations can be given for all muscles studied, as there was no aging by muscle interaction. An aging by USDA grade interaction was noted (P<0.05). The upper 2/3 of USDA Choice need not be aged beyond 7 d. USDA Select should be aged at least 14 d. Steak location within muscle had an effect (P<0.05) on WBSF values in four muscles. These data indicate that fabrication and merchandizing decisions should be made on an individual muscle basis.     

Sensory evaluation of beef-flavor-intensity, tenderness, and juiciness among major muscles

Twelve muscles from eight USDA Select/Choice grade steers were evaluated for beef-flavor intensity, tenderness, and juiciness. The biceps femoris, psoas major, gluteus medius, semimembranosus, and triceps brachii were similar in beef-flavor-intensity (P > 0·05) and were ranked as the most intensely flavored of all muscles. The rectus femoris, longissimus lumborum, serratus ventralis, infraspinatus, semitendinosus, pectoralis profundus, and supraspinatus generally were less intense in beef-flavor than the other muscles and were ranked from highest to lowest intensity in that order. The psoas major was the most tender (P > 0·05) followed by the infraspinatus, longissimus lumborum, and rectus femoris, which were similar (P > 0·05). Generally, muscles from the chuck and loin were juicier than those from the round.     

SODIUM LACTATE/SODIUM POLYPHOSPHATE EFFECTS ON OXIDATION IN PRECOOKED FROZEN PORK PATTIES

The objective of this study was to evaluate sodium lactate (SL; 0, 1, 2 or 3%) and sodium polyphosphate (SP; 0, 0.1, 0.2 or 0.3%) effects on lipid oxidation and color characteristics of precooked pork patties during frozen storage. Ground pork was mixed with SL and/or SP, stuffed, frozen, sliced and cooked from the frozen state, packaged and held frozen for 14 weeks. Samples containing SP had lower TBARS, regardless of SL content. SL decreased pH, a* and b* values and red color (R630–R580). L* value, hue angle, pork flavor, saltiness, and juiciness increased as SL increased. A moderate correlation occurred between SL and TBARS. SP decreased hue angle, cook loss, and rancid flavor and increased pH, b* value, pork flavor, saltiness, and juiciness. Storage time decreased a* value, red color and juiciness, while saltiness, alkalinity, and rancid flavor scores increased. Based on both physical and sensory characteristics, optimum combinations appear to be 3% SL and 0.2–0.3% SP.     

Effects of Sodium Tripolyphosphate on Physical and Sensory Properties of Beef and Pork Roasts

Six paired beef round and pork loin roasts were used to determine the effects of sodium tripolyphosphate (STPP) on fresh and reheated roasts. Roasts were pumped 10% of their weight with distilled water (control) or with distilled water containing 4.75% STPP. Roasts were cooked to internal temperatures of 70°C (beef) or 75°C (pork) and evaluated after 0, 1 or 3 days (roasts were reheated to cooked temperatures). This study indicates that phosphate in pork and beef roasts allowed them to be reheated after 1 and 3 days of refrigeration with minimal losses of juiciness, tenderness or flavor intensity, Phosphate injection reduced warmed-over flavor in reheated pork roasts but was not successful in decreasing the incidence of warmedover flavors in reheated beef roasts.     

Effects of quality grade on the chemical, physical and sensory characteristics of Hanwoo (Korean native cattle) beef

The effects of quality grade (which reflects relative marbling) on the chemical, physical and sensory properties was investigated using Korean native cattle (Hanwoo) beef. Thirty-six Hanwoo cows were slaughtered and the carcasses were graded at 24h postmortem according to the Korean carcass grading system. The quality grade 1 (high quality), grade 2 and grade 3 (low quality) were based on the marbling score of longissimus dorsi (LD) muscles. The effects of quality grade on the meat quality parameters of beef LD muscle were assessed during aging. Loin-eye area, fat thickness and yield grade were all similar for the three quality groups. Mean lean color, fat color and maturity scores did not differ among quality grade groups (P>0.05). pH, Sarcomere length, WHC, collagen content, cooking loss, shear force and MFI were not affected by quality grade groups. Drip loss for grade 1 group was significantly lower than that for grade 3 groups (P<0.05). At initial tenderness evaluation of steaks, no differences among the three grades were observed; however, with additional days of storage, grade 1 steaks had higher tenderness score than grade 3 steaks (P<0.05). Increased postmortem aging time improved tenderness attributes regardless of quality groups. No significant differences were found among the quality grade groups for flavor (P>0.05). The grade 1 group had the highest juiciness score, and grade 3 groups had the lowest score (P<0.05), but postmortem aging did not influence flavor and juiciness. The quality grades were more strongly related to juiciness than tenderness or flavor.     

Muscle Mineral Concentrations as Predictors of Taste Panel Sensory Attributes of Beef

M. longissimus concentrations of Zn, Co, Se, Cd, Mn, Na, Fe, Ca and Mg were used to predict taste panel sensory attributes of 144 beef rib roasts. The best equations included 4, 6, 2 and 5 predictors for flavor, juiciness, tenderness and chew test, respectively, and explained from 4.9 (tenderness) to 26% (flavor) of the variation. When all predictors were used, they explained 28.2, 25.6, 7.5 and 25.4% of the variation in flavor, juiciness, tenderness and chew test. Thus, muscle mineral concentration is as poor a predictor of sensory attributes as marbling score. If variation in taste panel sensory attributes of beef from young cattle is important, other predictors must be identified.     

Frozen storage stability of antioxidant-treated raw restructured beef steaks made from mature cows

Previous research has shown that beef quality decreased with the age of cattle. In this study, beef trimmings from nine mature cows (n = 9), equally representing three animal age groups (2–4, 6–8, and 10–12 yr), were restructured into steaks formulated with propyl gallate, alone or in combination with a beefy flavoring agent, to enhance palatability and stability during 6 months of frozen storage at −29 °C. Lipid oxidation, rancidity, and loss of beefy flavor in restructured steaks during extended storage were reduced by propyl gallate. The beefy flavoring agent inclusion masked mature, forage-fed beef off-flavors, intensified beefy flavor, and improved steak tenderness, juiciness and cooking yield. Thus, the combination of propyl gallate and beefy flavoring offers an effective means to enhance the palatability and storage stability of restructured beef prepared from mature cows.     

The influence of mechanical tenderization on the palatability of certain bovine muscles

Twelve different muscles or muscle groups were removed from both sides of 25 Canada AA beef carcasses. Muscles or muscle groups from alternate carcass sides were either mechanically tenderized or served as controls. Mechanical tenderization increased thaw-drip losses from the brisket approximately 1.25% and cooking losses from the blade eye by approximately 2.75%, but did not influence cooking times. Mechanical tenderization also improved initial and overall tenderness of the outside round, top sirloin, striploin, inside round and chuck tender. In addition initial tenderness of the sirloin tip eye and overall tenderness of the eye of the round were improved by mechanical tenderization. Moreover, connective issue in the top sirloin, striploin, and eye of the round was less perceptible after mechanical tenderization, but juiciness was decreased in the blade eye and top sirloin. Flavor intensity of the top sirloin and rib-eye was also reduced by mechanical tenderization. However, the desirability of the flavor of the inside round was improved by mechanical tenderization and the overall palatability of the inside round and eye of the round improved with mechanical tenderization. Mechanical tenderization decreased the proportion of inside round samples rated tough initially and overall from 52 to 20% and from 36 to 12% respectively. Mechanical tenderization also reduced the proportion of outside round samples perceived to contain a slight or greater amount of connective tissue from 36 to 12%. In addition, mechanical tenderization reduced the proportion of inside round samples with undesirable flavor from 16 to 0% and reduced the proportion of unpalatable rib-eye, inside round, and eye of the round samples from 12 to 0, 36 to 8 and 80 to 40%, respectively. Consequently, mechanical tenderization can be effectively utilized to reduce the variability and improve the tenderness and palatability of certain muscles, particularly hip muscles.     

Consumer evaluation of palatability characteristics of a beef value-added cut compared to common retail cuts

The objectives of this study were to educate consumers about value-added beef cuts and evaluate their palatability responses of a value cut and three traditional cuts. Three hundred and twenty-two individuals participated in the beef value cut education seminar series presented by trained beef industry educators. Seminar participants evaluated tenderness, juiciness, flavor, and overall like of four samples, bottom round, top sirloin, ribeye, and a value cut (Delmonico or Denver), on a 9-point scale. The ribeye and the value cut were found to be similar in all four attributes and differed from the top sirloin and bottom round. Correlations and regression analysis found that flavor was the largest influencing factor for overall like for the ribeye, value cut, and top sirloin. The value cut is comparable to the ribeye and can be a less expensive replacement.     

Effects of enhancing beef longissimus with phosphate plus salt, or calcium lactate plus non-phosphate water binders plus rosemary extract

Beef strip loins (n=36) were enhanced with a sodium phosphate plus salt solution (PS); or with a calcium lactate solution (Ca) plus 1% or 2% beef broth (Br) plus natural flavoring (N) containing rosemary extract; or with 1% or 2% kappa carrageenan (Cr) plus N to determine effects of ingredients on color life, water-binding ability, and palatability traits. Enhancement with PS resulted in higher pH, higher pumped yields, greater water-binding ability, and higher tenderness and juiciness scores than enhancement with Ca (all p<0.05). Enhancement with Ca resulted in less color deterioration, less metmyoglobin discoloration, higher L (?), a (?), and b (?) values, higher beef flavor intensity scores and lower off-flavor scores than enhancement with PS (all p<0.05). Warner-Bratzler shear values did not differ among treatments. Steaks enhanced with Br had less color deterioration, less metmyoglobin discoloration, and higher L (?) values than those enhanced with Cr (all p<0.05). Pumped yields were not different between loins enhanced with Br or Cr. The N flavoring containing rosemary extract decreased (p<0.05) discoloration. Enhancing beef longissimus with PS increased water-binding capacity and sensory tenderness traits, but reduced color stability and increased off-flavors, whereas Ca preserved color stability and enhanced flavor at the expense of pumped yields. The use of Br or Cr had no influence on tenderness or palatability traits.