Assessment of physico‐chemical properties of water and phytoplankton bloom formation in a eutrophic pond using multivariate analysis

An investigation of the water chemistry and phytoplankton bloom formation characteristics of a seasonal earthen pond located in the Alappuzha District, Kerala (India) was carried out during November 2011 to May 2012. Twelve important physico‐chemical parameters (temperature, Secchi disc transparency, pH, electrical conductivity EC), dissolved oxygen (DO), biochemical oxygen demand (BOD), ammonia‐nitrogen, nitrite‐nitrogen, nitrate‐nitrogen, total phosphorus (TP), dissolved iron and chlorophyll a related to phytoplankton growth in the pond were the focus of the present study. Chlorophyll a was used as a measure of phytoplankton biomass. The pond developed subsurface phytoplankton blooms of Chlorella vulgaris, Aphanothece sp., Leptosira sp., Lepocinclis globulus and Lepocinclis fusiformis. Visible scums of Lepocinclis globulus and Euglena proxima was also observed during March 2012. Principal component analysis was performed to understand the biochemical processes in the pond ecosystem leading to the Euglena spp. bloom.     

Phytoplankton composition and biomass in tropical soda Lake Shala: seasonal changes in response to environmental drivers

Although soda lakes are valuable, sensitive aquatic resources where phytoplankton play a decisive role for the entire ecological functions, they are among the least‐studied ecosystems. Seasonal variations in phytoplankton composition, abundance and biomass in relation to some environmental parameters of the little known, deep, large, volcanic and saline–alkaline Lake Shala were investigated over an annual cycle. The lake phytoplankton community consisted of relatively diverse taxa (23) belonging to Bacillariophyceae, Cryptophyta, Cyanoprokaryota and Dinophyta. Bacillariophyceae and Cryptophyta were the dominant groups throughout the annual cycle, accounting for about 57% and 22% of the total number of species, and 28% and 69% of the total abundance of the phytoplankton community, respectively. Cryptomonas spp. were most abundant throughout nearly all months, contributing about 59%–95% of total phytoplankton abundance, followed by Thalassiosira sp. (1%–35%). The chlorophyll‐a concentration, as a proxy for algal biomass, was generally low (mean 17 μg L^?1), exhibiting only small seasonal variation. The strong, inverse relation of chlorophyll‐a with water transparency (r = ?0.69; n = 11) and the persistent dominance of species adapted to low‐light conditions and mixing suggest the overriding importance of these factors in controlling the lake's phytoplankton. The results of the present study generally suggest the phytoplankton composition and biomass in Lake Shala exhibited muted seasonal changes, despite the environmental perturbations, probably because of the lake's high buffering capacity against allochthonous impacts because of its voluminous nature.     

Relative contribution of bacteria, algae and ciliates to the microplankton biomass in an eutrophic lake

Biomass in size-fractionated plankton (0.2–1.2 μm; 1.2–12/μm; 12–45 μm and 45–160 μm), together with that of ciliated protozoans (size fraction 1–160 μm), were assessed during stratification, in an eutrophic lake (Lake Aydat, Massif Central, France). Samples (51) were gathered from depths of 2, 7 and 14 m during 17 weekly samplings in the centre of the lake. Three biomass estimations were performed: cell-counts, chlorophyll a and ATP assays. Ciliates were counted live. Five species groups dominated the ciliate community: Loxodes striatus, Frontonia spp., Spirostomum ambiguum, Strombidium spp. and Dexiotrica plagia. Frontonia always had the highest cell concentration (54–64% of total cell counts), while the bulk of biomass was divided between Loxodes (42–70% of total) and Frontonia (29–53% of total). In terms of carbon, both the bacteria and ciliate contributions to the total microplanktonic biomass (size fraction 1–160 μm, i.e. bacteria + phytoplankton + ciliates) depends on the choice of the phytoplankton biomass estimation technique used. Thus, the contributions increase respectively from 16% and 19%, when phytoplankton biomass is estimated from chlorophyll α concentrations, to 24% and 27%, when phytoplankton biomass is based on biovolume. Relationships between bacteria, phytoplankton and ciliated protozoans reveal that ATP data are greatly overestimated by bacteria and ciliate interferences, especially in the metalimnion and hypolimnion. In the epilimnion, this interference is less. Interpretations of phytoplankton dynamics and metabolic activity from ATP assays should be made with extreme care, especially in eutrophic environments.     

Optical and biological properties of Lake Ülemiste, a water reservoir of the city of Tallinn I: Water transparency and optically active substances in the water

Optical and biological measurements were performed in Lake Ülemiste in the summer of 1997 (four measurement days), and from May to October in 1998 (12 measurement days). This kind of data, describing the type and amount of optically active substances in the water, phytoplankton characteristics, the underwater light field, and temperature–oxygen situation in the lake are necessary when estimating the ecological state of the lake. Lake Ülemiste is the main drinking water reservoir of Tallinn, the capital of Estonia. Phytoplankton abundance and biomass, chlorophyll a and suspended matter were determined from collected Lake Ülemiste water samples in the laboratory. Spectrophotometrical processing of the filtered and unfiltered water was carried out to describe the beam attenuation coefficient spectra and optical influence of yellow substance in the water. Vertical profiles of downwelling irradiance of the PAR (400–700 m) region of the spectrum (and from these data the averaged over depth diffuse attenuation coefficient) were determined. The relative transparency of the water was estimated by using a Secchi disk. Passive optical remote measurements were episodically made from aboard a boat. Results obtained confirmed that Lake Ülemiste is turbid (almost hypertrophic), comparable with most turbid lakes in Estonia (e.g. Lake Võrtsjärv) and Finland (Lake Tuusulanjärvi). Its chlorophyll a content varied within the range 13–121 mg m^–3, phytoplankton biomass 3–107 mg L^–1, phytoplankton abundance 65 000–999 000 mL^–1, suspended matter 8–34 mg L^–1, effective concentration of the yellow substance 6–30 mg L^–1, diffuse attenuation coefficient of light in the PAR region 1.0–3.3 m^–1 and a Secchi disk depth of 0.5–1.75 m. The temporal variation of the spatial averages of these parameters during 1998 was analysed. Almost all characteristics showed an increase from May to midsummer with a maximum in late July or in August (correspondingly the Secchi depth values were minimal in late summer). The amount of yellow substance was an exception, which was nearly constant during the observation period. The maximum level of chlorophyll a content in July and August 1998 markedly exceeded that in 1997, despite the fact that the summer of 1997 was sunny, but the summer of 1998 was cold and rainy. The correlative relationships between the different parameters were investigated together with the respective data for other lakes. They show that the data of Lake Ülemiste supplemented the correlation graph in the region of turbid lakes, whereby in all cases the correlation coefficient increased following the addition of Lake Ülemiste data. The highest correlation coefficient was obtained when light attenuation coefficient values were correlated with a sum of weighted concentrations of chlorophyll a, yellow substance and suspended matter (multiple regression analysis).     

Restoration of small lakes through cooperative management: A suitable strategy for poverty‐laden areas in developing countries?

Lake Rupa is a small, subtropical, shallow lake with a surface area of 100 ha situated 600 m a.s.l. in Central Himalaya, Nepal. This degraded lake was studied between 2000 and 2006, with the goal of determining whether or not it could be restored by a community‐based cooperative of local people living in its catchment. Main threats to the lake, its aquatic life and its very existence include encroachment by excessive aquatic vegetation, sedimentation, and low in‐lake concentrations of dissolved oxygen (DO). Small lakes (≤ 500 ha) are relatively prone to the process of ‘succession and climax’, although they also can be of social, environmental and ecological importance. Thus, their disappearance could spark social chaos and disorder in areas already having to cope with other rapid environmental changes. This means that credible mechanisms for revitalizing or protecting small lakes are an important goal. Accordingly, a lake cooperative of 329 households living in close vicinity to Lake Rupa was formed in 2002, by prioritizing traditional fishers, women and other deprived community members with the goals of respecting the citizenry and equity. Following the cooperative's campaigns directed at weed removal and fish stocking, encroachment of vegetation on the lake margins was halted, its aquatic weeds became under control, and its fisheries improved. In addition to the cooperative's restoration activities, several water quality parameters, including water transparency and DO and chlorophyll‐a concentrations, were monitored on a monthly basis. The measured water transparency was inconsistent, exhibiting large variations between 2000 and 2003. Relatively low, but consistent, values, however, were measured after 2004. The results imply that the removal of weeds, and sufficient nutrients and solar radiation are subsequently available to the lake's phytoplankton communities. Supporting this notion is that the chlorophyll‐a concentration spiked to 205 µg L⁻¹ in November 2006, the water transparency became consistent, and the DO concentration increased to >3.8 mg L⁻¹ during the critical months (March–May) after 2004. These water quality indicators indicated improvement in the degraded Lake Rupa, suggesting that the establishment of cooperatives such as that highlighted in this study could be a powerful and sustainable mechanism for restoring degraded lakes in similar socioeconomic settings by maintaining equity, by connecting communities with their resources, and by facilitating integrity, equity, citizenry and social justice.     

Spatial variations in nutrients and other physicochemical variables in the topographically closed Lake Baringo freshwater basin (Kenya)

Spatial physicochemical parameters were determined from 39 sampling sites distributed throughout Lake Baringo during December 2010. Mean values of temperature, dissolved oxygen concentration and electrical conductivity decreased successively with depth, while the pH remained constant. Only the turbidity values increased marginally with depth. Of the surface water parameters, mean (range) values of dissolved oxygen (DO), pH, electrical conductivity, water transparency and turbidity were 6.9 (4.5–8.4) mg L^?1, 8.3 (7.8–8.5), 573 (556–601)μS cm^?1, 33 (28–37) cm and 43.3 (32.7–54.6) NTU, respectively. Mean and range values of total nitrogen (TN), nitrate‐nitrogen(NO₃‐N), ammonia nitrogen (NH₄‐N), total phosphorus (TP) and soluble reactive phosphorus (SRP) were 788.4 (278–4486) μg L^?1, 4.5 (2.4–10.0) μg L^?1, 42.6 (33.8–56.3) μg L^?1, 102.9 (20.3–585.3) μg L^?1 and 23.5 (15.2–30.5) μg L^?1, respectively. Dissolved silica concentrations ranged from 19.7 to 32.7 mg L^?1, with a mean value of 24.7 mg L^?1. The chlorophyll‐a concentrations were quite low, ranging from 1.4 to 4.9 μg L^?1, with a mean value of 4.2 μg L^?1. In contrast to previous reported values, a key finding in the present study is a relatively high water transparency, indicating a relatively clear water column, due possibly to the fact that the sampling was conducted during the dry period. The nutrient levels remained low, and the chlorophyll‐a concentration also was an almost all time low value. A TP value of 20 μg L^?1 and higher confirms strongly eutrophic conditions prevailing in the lake, with an extremely low potential for fish production and low species diversity, consistent with other studies. The results of the present study, therefore, reinforce the database for future management and monitoring plans for the Lake Baringo ecosystem, which lies adjacent to known geothermally active zones and a saline Lake Bogoria.     

Effects of temperature and nutrients on phytoplankton biomass during bloom seasons in Taihu Lake

Long-term variations of phytoplankton chlorophyll-a (Chl-a), nutrients,and suspended solids (SS) in Taihu Lake, a large shallow freshwater lake in China, during algal bloom seasons from May to August were analyzed using the monthly investigated data from 1999 to 2007. The effective accumulated water temperature (EAWT) in months from March to June was calculated with daily monitoring data from the Taihu Laboratory for Lake Ecosystem Research (TLLER).The concentrations of Chl-a and nutrients significantly decreased from Meiliang Bay to Central Lake. Annual averages of the total nitrogen (TN), total phosphorus (TP), and Chl-a concentrations, and EAWT generally increased in the nine years. In Meiliang Bay, the concentration of Chl-a was significantly correlated with EAWT, ammonia nitrogen (NH4+-N ), TN, the soluble reactive phosphorus (SRP),TP, and SS. In Central Lake, however, the concentration of Chl-a was only correlated with EAWT, TP, and SS. Multiple stepwise linear regression revealed that EAWT, dissolved total phosphorus (DTP), and TP explained 99.2% of the variation of Chl-a in Meiliang Bay, and that EAWT, NH4+-N, and TP explained 98.7% of the variation of Chl-a in Central Lake. Thus EAWT is an important factor influencing the annual change of phytoplankton biomass. Extreme climate change, such as extremely hot springs or cold springs, could cause very different bloom intensities in different years. It is also suggested that both nutrients and EAWT played important roles in the growth of phytoplankton in Taihu Lake. The climate factors and nutrients dually controlled the risk of harmful algal blooms in Taihu Lake. Cutting down phosphorus and nitrogen loadings from catchments should be a fundamental strategy to reduce the risk of blooms in Taihu Lake.     

Eutrophication of lakes in urbanized areas: The case of Yaounde Municipal Lake in Cameroon, Central Africa

A 1 year qualitative and quantitative evaluation of phytoplankton and chlorophyll‐a, as well as some physicochemical parameters, was recorded in a shallow tropical lake in Cameroon: the Yaounde Municipal Lake. Physicochemical measurements also were regularly made in its main tributary (Mingoa Stream). These analyses aimed to assess the lake's trophic status and to propose measures for controlling its degradation process. The Secchi disk transparency was low and rarely exceeded 100 cm. Conductivity was higher near the lake bottom. The oxygen deficiency, and sometimes anoxia, recorded from a 2.5 m depth leads to high quantities of ammonium‐nitrogen. The total phosphorus concentrations varied from 80–2290 µg P L^?1 and the total Kjeldhal nitrogen concentrations fluctuated between 3 and 15 mg  L^?1. Upstream to the lake, in the Mingoa Stream, total phosphorus concentrations ranged from 0.6–3.8 mg P L^?1 and total Kjeldhal nitrogen concentrations ranged from 10–22 mg  L^?1. There are up to 102 phytoplankton‐specific taxa, with Euglenophyta and Chlorophyta particularly more diversified. The phytoplankton biomass and chlorophyll‐a concentrations reached 225 µg mL^?1 and 566 mg m^?3, respectively. The analyses pointed out the allogenic nature of the functioning of this ecosystem as a result of bad waste management in the surrounding landscape. Urgent actions need to be undertaken in order to rehabilitate this lake, which rapidly shifted to a hypertrophic status.     

Response of phytoplankton from the metalimnetic chlorophyll maximum to macro- and micro-nutrients amendments in Lake Geneva

Despite re-oligotrophication and low trace elements concentrations, direct determination of limiting nutrient(s) of the phytoplankton community in Lake Geneva is still missing. Incubation experiments were carried out with either addition of phosphate (+P), nitrate (+N), reactive silica (+Si), iron (+Fe), nickel (+Ni) and molybdenum (+Mo) to surface water collected between 9 and 12?m, during seasonal fieldtrips in 2015. Macro- and micro-nutrients, dissolved concentrations, temperature and irradiance as well as chlorophyll a (Chl a) levels, Chl a:POC ratios, POP:POC ratios and Chl a variable fluorescence parameters were quantified. The rapid phosphorus consumption, elevated inorganic nitrogen to phosphorus ratios and increased Chl a, Chl a:POC and POP:POC, in response to P additions, all indicated a phosphorus limitation in phytoplankton. Moreover, none of the measured parameters differed significantly from the control in the other treatments. These results were also confirmed by the response of Chl a variable fluorescence. The effective photosystem II quantum yield in ambient light, the absolute electron transfer rate and the connectivity, under 53?μmol photons/m²/s illumination, significantly increased while the non-photochemical quenching decreased in the +P treatment. Phytoplankton cells supplemented with phosphate were thus more efficient to capture photons and to drive them into photochemistry at light levels close to in situ levels. This is the first study to demonstrate directly a phosphorus limitation in Lake Geneva, pointing towards the success of its re-oligotrophication process.     

Diversity and ecology of phytoplankton in Lake Edward (East Africa): Present status and long-term changes

Lake Edward is one of the African Rift Valley lakes draining into the Nile River basin. We conducted three sampling series in Lake Edward in October-November 2016, March-April 2017 and January 2018, in distinct seasonal conditions and in several sites varying by depth and proximity to river outlets, including the Kazinga Channel, which connects the hypertrophic Lake George to Lake Edward. The phytoplankton was examined using microscopy and marker pigment analysis by high performance liquid chromatography (HPLC) and subsequent CHEMTAX processing for estimating abundance of phytoplankton groups. Chlorophyll a concentration in the pelagic and littoral open lake sites barely exceeded 10 µg L⁻¹ whereas, in contrast, in the semi-enclosed Bay of Katwe influenced by the Kazinga Channel chlorophyll a was up to 100 µg L⁻¹. Despite substantial seasonal variations of limnological conditions such as photic and mixed layer depths, cyanoprokaryotes/cyanobacteria represented on average 60% of the phytoplankton biomass, followed by diatoms, which contributed ~25% of chlorophyll a, and by green algae, chrysophytes and cryptophytes. 248 taxa were identified with clear prevalence of cyanobacteria (104 taxa), from the morphological groups of coccal and filamentous species (non-heterocytous and heterocytous). The high proportion of heterocytous cyanobacteria, along with a relatively high particulate organic carbon to nitrogen (C:N) ratio, suggest N limitation as well as light limitation, most pronounced in the pelagic sites. During the rainy season, the most abundant diatoms in the plankton were needle-like Nitzschia. Comparison with previous studies found differences in water transparency, total phosphorus, and phytoplankton composition.     

Lake—Wide Seasonal Changes in Limnological Conditions in Lake Michigan in 1976

Data collected on lake-wide cruises in 1976 were used to study seasonal and vertical variations in water temperature, transparency, chlorophyll a, and nutrients in Lake Michigan. Data were analyzed according to subsets corresponding to the northern and southern open lake. Comparisons (t-tests) of data from the open lake indicated that the average water temperature was cooler and average water transparency was greater in the northern lake than in the southern, but with the exception of total phosphorus, average nutrient concentrations did not differ between the northern and southern parts. It was found that physical-chemical characteristics of nearshore and Straits of Mackinac stations differed significantly from open lake stations. Seasonal phytoplankton dynamics in the open lake were related to seasonal and vertical changes in silica and nitrate nitrogen. The spring phytoplankton bloom occurred before the lake was strongly stratified thermally. After thermal stratification was well developed, epilimnetic concentrations of chlorophyll a decreased, probably due to some combination of nutrient limitation and zooplankton grazing, and maximum chlorophyll a concentrations were found below the thermocline. Epilimnetic silica concentrations decreased after thermal stratification and diatoms were replaced in the phytoplankton assemblage by green and blue-green algae in late summer. Total phosphorus averaged only 8 μg/L on a lake-wide basis and thus only small but significant reductions in absolute concentration can be expected from phosphorus control programs. However, over a period of several years, these small reductions in concentration may be difficult to verify from total phosphorus measurements which have relatively large sampling errors and variances.     

Bacterioplankton heterotrophic activity in relation to the phytoplankton compartment in a recently formed reservoir

Seasonal and spatial variations in bacterial abundance, biomass and potential heterotrophic activity in a recently flooded reservoir were measured for two consecutive years, in conjunction with phytoplankton biomass (chlorophyll‐a concentration) and activity (primary production). The mean value of primary production remained constant between the two study years, while those of the chlorophyll‐a concentrations, bacterial abundance, bacterial biomass and bacterial heterotrophic activity decreased. The observed trends in the bacterial variables were linked to changes in the relative importance of allochthonous dissolved organic matter, in addition to selective grazing activity on bacterioplankton. Multivariate regression analyses identified bacterial abundance (29%) and temperature (17%) as dominant correlates of the bacterial potential heterotrophic activity. We concluded that, in a new reservoir, organic matter, other than that from phytoplankton, might be of great importance for bacterioplankton metabolism. Furthermore, grazing activity on bacteria by metazoa in a new reservoir represents, on occasion, an important trophic link between the top consumer and otherwise unavailable dissolved organic carbon sources. Finally, even if little energy is transferred to larger consumers, the microbial route is still important in re‐mineralizing organic matter in Sep Reservoir.     

Chemical, physical and biological characteristics of Saladito Reservoir, Cienfuegos Province, Cuba

Saladito Reservoir, located in Cienfuegos Province in Cuba, lies in an intermediate position between tropical and subtropical climatic conditions. Although Cuba only had 13 reservoirs before 1959, the government has constructed 229 reservoirs since that time, with a total storage capacity of 8.81 km³, administered by the National Hydraulic Resources Institute. In 1995, it was proposed to use waste water from the Swinish Farm to fertilize small reservoirs, providing nitrogen and phosphorus to increase primary production in the reservoirs as a means of facilitating high fish production levels. Saladito Reservoir, with a hydrographic basin of 34.5 km², a mean depth of 3.4 m and a water retention time of 2 years, was constructed as the final part of the wastewater treatment system for Swinish Farm in the Palmyra Municipality. The main objective of this study is to determine the level of eutrophication in the reservoir after it had received waste water from the Farm for 17 months, based on relevant biological (phytoplankton), chemical and physical indicators sampled at five stations on 22 and 23 May 1995. Based on the measured parameters, the concentration of dissolved salts was generally high, with calcium and bicarbonate being the dominant ions. The nitrite‐nitrogen and nitrate‐nitrogen concentrations were relatively low at the surface, increasing with depth, while the ammonium‐nitrogen concentrations were extremely high, likely related to the high organic content of the reservoir. The total phosphorus concentration also exhibited high values, indicating the possible existence of soluble phosphorus in a complex with iron, which would allow algae to assimilate it like phosphate. The water transparency, measured as Secchi disk, was 0.2 m at all sampling stations. The chlorophyll‐a concentration ranged from 111 µg L⁻¹ in the surface water to 50 µg L⁻¹ at the bottom depth. The phytoplankton cell counts identified 27 algal species contained in five classes. The dominant group was Cyanophyceae, with Oscillatoria sp. being the largest contribution to the reservoir biomass. Twenty‐seven taxa were present in the reservoir in concentrations of at least 1 mg m⁻³. The results of this study indicate that Saladito Reservoir is hyper‐eutrophic, based on its measured biomass (cell counts), and chlorophyll‐α, inorganic nitrogen and total phosphorus concentrations. The possible production of algal toxins from blue‐green algal growths also could be affecting the productivity of Saladito Reservoir.     

Phytoplankton primary production, chlorophyll-a and nutrient concentrations in the water column of mountainous Lake Phewa, Nepal

The seasonal patterns of phytoplankton primary production, chlorophyll‐a concentration, cell number and several other limnological variables in Lake Phewa, located in the active monsoon zone in Central Himalaya, Nepal, were studied for a year beginning in April 2001. During the study period, the gross primary production and chlorophyll‐a concentrations were relatively low during the monsoon season. The phytoplankton cell number, represented by 24 genera, also fluctuated seasonally, but tended to increase in the pre‐ and post‐monsoon period. These results suggest that the monsoon plays a crucial role in the primary production and phytoplankton dynamics for Lake Phewa. Among the phytoplankton species, Microcystis aeruginosa, a representative species for eutrophic lakes, was the dominant phytoplankton. At the same time, however, it is clear that the lake is not yet heavily eutrophic. The present study suggests that the exchange of lake water during the monsoon season contributes to maintaining the health of the lake against further degradation. Nevertheless, the silt carried in the monsoon rain run‐off from the lake's catchment area suggests increasingly serious degradation problems for this small mountainous lake.     

Regime shifts observed in Lake Kasumigaura,a large shallow lake in Japan: Analysis of a 40‐year limnological record

Lake Kasumigaura, which is composed of the two basins (Nishiura and Kitaura), is a large, shallow, hypereutrophic lake. Phytoplankton and water quality records from the past forty years were analysed to elucidate whether or not, when, and what type of certain regime shifts may have occurred, based on using inferential regime shift detectors. Characteristics of the phytoplankton and water quality changes were similar at 6 sampling sites in the two basins, with 20 water quality parameters being classified into four groups, based on cluster analysis. Shifts in dominant plankton groups (DPGs) and water quality occurring almost concomitantly, concentration on the period from 1987 to 1992 (Shift A) and from 1997 to 2001 (Shift B), with those observed for the two basins usually being similar with small differences. Two types of inferential regime shift detectors (sequential t‐test type; Rodionov's RSD and sequential F‐test type: package strucchange in R) yielded similar timings and significances of the shifts. Furthermore, changes in skewness and conditional heteroskedasticity (package early warnings in R) usually represented early warning signals before the shifts. Correlation analysis and ratios of dissolved inorganic nitrogen (DIN) vs. total phosphorus (TP) supported the hypothesis that phosphorus was the phytoplankton biomass limiting nutrient, except for one period for the Nishiura basin. Neither the nitrogen:phosphorus (N/P) ratio hypothesis and ammonia–nitrate (NH₄‐N/NO₃‐N) hypothesis satisfactorily explain the DPGs before and after Shift A (Microcystis spp. and Planktothrix spp., respectively), although it may be possible that these ratios triggered the DPG change in this shift. A considerable increase in silicon was observed for Shift B when the DPGs changed from cyanobacteria to diatoms. Further studies on the accurate types and triggers of the regime shifts are necessary to better understand the interactions between ecosystem and water quality for this and similar lakes elsewhere.     

Life under ice in the perennial ice‐covered Lake Glubokoe in Summer (East Antarctica)

This study focuses on hydrological and biotic variables in Lake Glubokoe, which is located in Thala Hills of Enderby Land (East Antarctica). Water and sediment samples and physical measurements were collected once a week in the austral summer (19 December 2010 – 6 February 2011). This lake exhibits perennial ice cover that reached a thickness of 2.5–2.7 m during the study period. A very low concentration of planktonic chlorophyll‐a (0.06–0.45 μg L^?1) was measured in the lake, indicating its ultra‐oligotrophic status. The water was poorly populated by algae and metazoans, especially in upper waters below ice cover to a depth of 2 m. Small planktonic organisms (2–5 μm) were observed throughout the study period, but larger organisms (>8 μm) such as the cyanobacteria Planktolyngbya limnetica occurred only during the warmest period (January). Only few individuals of metazoans (rotifers) were found in planktonic samples. Due to deep light penetration (10–15% of incoming active solar radiation reached the depth of 30 m), thick cyanobacterial mats (30 cm) cover all the bottom surface (grey silts) in the lake. Abundant benthic biota associated with these mats was found (up to 1000 ind. m^?2). Among the benthic metazoans, bdelloid rotifers and tardigrades were the dominating taxa. The results of this study suggest a typical ecological feature of most subglacial lakes in East Antarctica is that metazoans are very poor in the pelagic zone, preferring instead to occupy an area near the lake bottom because of a favourable constant temperature of 4 °C, good level of dissolved oxygen and available food resources as the bacterial detritus.     

Seasonal and diel changes of dissolved oxygen in a hypertrophic prairie lake

Humboldt Lake, a hypertrophic prairie lake typical of many found on the Great Plains of North America, is usually ice‐covered from early November to about mid‐May. The lake is an important recreational fishery, now mainly stocked with walleye. It has a high potential risk of experiencing fish kills because of the very large cyanobacterial blooms that develop in it, the high rates of algal and bacterial production and the high concentrations of ammonia (NH₃‐N) and dissolved organic matter. Following the collapse of cyanobacterial blooms, shallow prairie lakes are known to undergo periods of anoxia that can lead to summer fish kills. In some of the lakes, anoxia forms during the long period of ice cover, causing winter fish kills. Two years of seasonal and diel data (total phosphorus, dissolved oxygen (DO), NH₃‐N and chlorophyll‐a concentrations, and bacterial production) were analysed in this study to assess why significant fish kills did not occur during this period or during the ≈ 30 years of records from Saskatchewan Environment. Humboldt Lake did not become anaerobic, either following the collapse of the cyanobacterial bloom or under ice cover, indicating that the oxygen (O₂) influx (strong mixing) and production processes were greater than the microbial and chemical O₂ demands, both over seasonal and diel time scales. Several published risk threshold criteria to predict the probability of summer and/or winter fish kills were applied in this study. The threshold criteria of maximum summer chlorophyll and maximum winter NH₃‐N concentrations indicated that a summer fish kill was unlikely to occur in this hypertrophic prairie lake, provided its water quality remained similar to that during this study. Similarly, the threshold criteria of initial DO storage before ice cover and the rate of O₂ depletion under ice cover also indicated a winter fish kill was unlikely. However, recent development in the watershed might have resulted in significant water quality deterioration and the winter fish kill that occurred in 2005.     

Limnological characteristics and trophic state of Paso de las Piedras Reservoir: An inland reservoir in Argentina

The current trophic status of Paso de las Piedras Reservoir was evaluated by analysing different physicochemical, biological and environmental variables, in relation to its water quality, and application of two different classification criteria. Water quality sampling was conducted at four sampling sites from June 2004 to June 2005. During this study, 183 phytoplankton taxa were identified. The phytoplankton abundance exhibited a maximum of 368.037 × 10³ cells.mL^?1 in February 2005, and at least 1.133 × 10³ cells.mL^?1 in October 2004. An almost exclusive dominance of Cyanobacteria was observed between December 2004 and May 2005, the product of a large relative abundance of Anabaena circinalis and Microcystis natans which, together with other companions, were the reason for an algal bloom characterized by an average density of 133.05 × 10³ cells.mL^?1, and an average chlorophyll concentration of 28.7 mg.m^?3. These study results indicate that the seasonal variations of physical, chemical and biological parameters in the waters of this reservoir were essentially a consequence of environmental and hydrological conditions in the dam area. In contrast, the spatial variations inside the lake were the result of the characteristics of the water inflow provided by its two main tributaries. The N:P ratio suggests neither nutrient is a limiting factor for maximum algal biomass in the lake, indicating that variations in the phytoplankton community structure, and development of phytoplankton blooms, would be more constrained by environmental and hydrological conditions than nutrient competition. The high concentration of measured nutrients could be attributed to the concurrence of various non‐point sources. The phytoplankton species richness was high, exhibiting values even higher than those mentioned in previous studies. Considering the two trophic classification systems, and based on total phosphorus data, the reservoir is classified within the hypertrophic category. In contrast, considering only the chlorophyll and turbidity data, the lake would be classified within the eutrophic category.