RHEOLOGY OF WHEAT DOUGHS FOR FRESH PASTA PRODUCTION: INFLUENCE OF SEMOLINA-FLOUR BLENDS AND SALT CONTENT

Dynamic measurements were made with a controlled stress rheometer to study the viscoelastic properties (G', G', δ) of wheat doughs (45% wb water content) for fresh pasta production prepared with different blends of durum wheat semolina and common wheat flour with different concentrations of sodium chloride. Increasing the semolina and sodium chloride content, increased the strength and the solid-like behaviour of semolina-flour blends. The physical properties of dough were strongly dependent on particle size distribution and salt addition. By manipulating semolina-flour ratio and ionic strength, it was possible to obtain semolina-flour doughs with a rheological behaviour close to that of pure semolina dough.     

A COMPARISON OF THE FARINOGRAPH AND A MODIFIED SHEAR-PRESS FOR MEASURING THE RHEOLOGICAL PROPERTIES OF DOUGH AND ITS FINISHED PRODUCTS1

The development of a single texture testing instrument for determining the complicated properties of dough and the textural characteristics of the final product of dough will eliminate the need for a series of specialized instruments. The farinograph and a modified shear-press were used to determine the rheological properties of doughs made from 15 mixtures of all purpose wheat flour, hard wheat flour and 6% soy fortified wheat flour adjusted with 54.7, 58.0, 61.1, 64.6 and 67.9% water absorptions. The shear-press was also used to determine the textural characteristics of the finished products. The shear-press was found to be equal to the farinograph in providing information on certain rheological properties of dough, and high correlations were obtained between these ‘texturegrams’ and farinogram parameters. For instance, the correlation between “viscoelasticity” per texturegram and “maximum consistency” per farinogram was 0.982; between “viscoelasticity” per texturegram and “elasticity” per farinogram was 0.969; and the correlation between “stability of viscoelasticity” per texturegram and “twenty minute drop” per farinogram was 0.941. The adhesive and cohesive properties of dough obtained from shear-press texturegrams explained 88.4% of the variance of the hardness of the end-products. The consistency and elasticity of dough obtained from farinograms explained 90.6% of the variance of the hardness of the end-products. If the method of water absorption and mixing of flour could be standardized, the shear-press could be utilized both for predicting rheological properties of bakery products from determinations on doughs as well as direct evaluations of the finished products.     

DYNAMIC VISCOELASTIC PROPERTIES OF SOY ISOLATE DOUGHS

The linear viscoelastic properties of soy isolate doughs have been determined as a function of flour concentration and temperature using a Rheometrics Mechanical Spectrometer. The material of lowest concentration (15%) which is classified as a dispersion rather than a dough exhibited values of storage (G') and loss moduli (G") which increased with increasing temperature above 50°C. On the other hand, values of G' and G" for 20% and 25% doughs showed only a slight increase with increasing temperature. The shape of the G' and G" versus frequency (ω) curves is suggestive of solid-like behavior for the doughs. The shape of these curves does not change with temperature which indicates there is very little change in the ability of the protein molecules to undergo configurational rearrangements as the result of heating. The phenomenon of gelation does not arise in the doughs but only in the dispersion of lower concentration. The data indicate that the bonds between protein molecules are noncovalent in nature and that the process of cooking soy dough does not involve the formation of crosslinks.     

THE EFFECT OF PROTEIN FORTIFICATION ON THE ACCEPTABILITY AND RHEOLOGICAL PROPERTIES OF COOKIE DOUGH AND ITS FINAL PRODUCT1

All-purpose wheat flour and all-purpose wheat flour fortified with 6% soy flour; 2, 4 and 6% single cell protein (SCP) were used. Protein quantity was increased significantly in both flour and cookies. Sensory panel evaluations were conducted for flavor, texture and overall acceptability of cookies, and objective evaluation of rheological properties were made with the farinograph and shear-press on doughs, and shear-press on cookies. Fortification with SCP at all levels altered all the sensory parameters significantly, but fortification with soy flour altered only cookie texture. Fortification with both SCP and soy flour significantly decreased the “spread factor” quality. Correlations between panel texture scores and texture parameters from the farinograph or the shear-press were not significant. However “Consistency” and “Elasticity” obtained from farinograms were significantly increased due to the high level of fortification by SCP and soy flour, and the same results were found in “Viscoelasticity” obtained from the shear-press. Also, the determinations from both instruments showed that the tolerance to kneading was significantly reduced as a result of the fortification. Adhesive properties of dough as obtained from texturegrams were significantly increased due to the 5% SCP and soy flour fortifications. The variations of textural characteristics were highly predictable from texture parameters of doughs as obtained from both instruments. Thus the coefficient of determination (R²) of crispness and hardness as measured by the shear-press were 0.987 and 0.999 when farinogram parameters were used, and 0.999 and 0.997 when texturegram parameters were used as independent variables.     

Prediction of the rheological properties of wheat dough by starch-gluten model dough systems: effect of gluten fraction and starch variety

The present study sought to investigate the rheological properties of wheat starch-gluten (WS-G) and potato starch-gluten (PS-G) model doughs with different gluten fractions to elucidate the effectiveness of using model dough to predict wheat dough properties. The highest linear viscoelastic region, frequency dependence, maximum creep compliance and the lowest viscoelastic modulus and zero shear viscosity were observed in the wheat dough, followed by WS-G and PS-G model doughs. PS exerted a more significant damage effect on the gluten network while WS shared a tight integration with gluten protein, forming a more stable dough structure. The viscoelasticity of the model doughs shared a close association with the wheat dough under increased gluten fraction, while the frequency dependence of the model doughs showed no trend towards wheat dough. Therefore, starch-gluten model dough could not fully stimulate the functionality of wheat dough irrespective of its gluten fraction.     

Effect of Carob Flour Addition on the Rheological Properties of Gluten-Free Breads

In this study, the rheological properties of gluten-free doughs from rice flour containing different amounts of carob flour were investigated. Water added changed in response to the carob amount. Dynamic oscillatory and creep tests were performed in order to gain knowledge on the rheological behaviour of doughs, which is essential for the control of the bread-making procedure and the production of high-quality bread. Simple power law mathematical models were developed in order to evaluate the effect of carob and water added in dough rheological behaviour. Creep data evaluation demonstrates that an increase in water content decreased the resistance of dough to deformation and, therefore, dough strength, whereas carob flour increased the elastic character and structure strength of the dough. This was also found in dynamic oscillatory tests. Increased amounts of carob flour led to an increase in bread dough elastic character since fibre addition elastifies and strengthens the dough structure. Moreover, doughs exhibited a solid-like viscoelastic character, with the storage modulus (G′) predominant over the loss modulus (G″). Dough rheological properties have an important effect on baking characteristics. Rheological experiments and applied mathematical models can provide us with good knowledge of rheological behaviour and dough viscoelasticity prediction. Therefore, dough samples containing carob-to-water ratios of 10:110 and 15:130 can be considered to possess a balance between the viscous and elastic properties compared to the other samples.     

INFLUENCE OF THE STARCH GRANULE SURFACE ON THE RHEOLOGICAL BEHAVIOUR OF WHEAT FLOUR DOUGH

The influence of the properties of the starch granule surface on the rheological behaviour of wheat flour doughs was studied in dynamic oscillation measurements (frequency sweep and strain sweep) and in stress relaxation measurements. A flour with a high protein content (15%) was diluted with wheat starch to obtain a protein content of 10%. The granule surface of the substituted starch was modified in three different ways: by heat treatment, by adsorption of a wheat protein fraction and by adsorption of lecithin to the granule surface. The effects of these modified starches were compared with the results obtained for nonmodified starch and protein or lecithin (in liposomes) added to the flour. Owing to the low concentration of the added protein and lecithin, no effect was observed when they were added to the bulk of flour. However, as a starch-surface modification the same components influenced the rheological parameters studied. Also the heattreated starch had an effect on the rheological behaviour. The study established the importance of the properties of the starch-granule surface in wheat flour dough.     

Rheological and Breadmaking Properties of Wheat Flours Supplemented with Octenyl Succinic Anhydride-Modified Waxy Maize Starches

The feasibility of emulsifying starches as bread improvers was investigated by incorporating starch sodium octenyl succinate (OSA-st), pre-gelatinized OSA-st and hydrolysed spray-dried OSA-st at 2.5, 5 and 10 % into wheat flour. Dough rheological properties (creep and recovery measurements; Mixolab, Alveograph) and bread quality parameters (specific loaf volume, crust and crumb colour, crumb moisture, crumb grain features, texture) were evaluated. The substituted flours, except hydrolysed OSA-st, significantly increased water absorption measured by Mixolab. The study on the rheological behaviour of doughs containing emulsifying starches, performed using a rheometer and an Alveograph, showed that OSA-st incorporation yielded strengthened dough, whereas pre-gelatinized and hydrolysed OSA-st addition led to more extensible dough. With regard to the thermal behaviour, investigated in water-limited systems by Mixolab, doughs prepared from pre-gelatinized OSA-st and hydrolysed OSA-st exhibited lower maximum peak torque, whilst all three examined starches increased cooking stability and decreased the setback value. Specific volumes of loaves baked from the substituted flours increased, and the highest effect was observed with pre-gelatinized OSA-st, which consequently produced bread crumbs with the largest mean gas cell area. The bread crumbs baked with octenyl succinate starches were whiter and softer. Although upon 1 day of storage no significant moisture retention capacity of emulsifying starches was noticed, the firmness values of OSA-st and pre-gelatinized OSA-st-supplemented bread crumbs, after 24 h of storage, were similar to or significantly lower than those of the control determined 2 h after baking. The obtained results indicate a requirement for further optimization of the octenyl succinate starch-supplemented doughs in terms of the combination of different types and levels of modified starches in order to obtain maximum bread quality.     

Influence of water and barley β-glucan addition on wheat dough viscoelasticity

The effects of the addition of two barley β-glucan isolates (0.2–1.0% of wheat flour), differing in molecular weight, and water (53–63% in a poor breadmaking wheat flour, cv. Dion, and 58–68% in a good breadmaking wheat flour, cv. Yekora) on the viscoelastic properties of wheat flour doughs were investigated. A response surface model (CCF) was used to evaluate the effects observed on the dynamic and creep-recovery parameters of the dough. The evaluation was done separately for each combination of β-glucan isolate (BG1 of ～10⁵ Da and BG2 of ～2 × 10⁵ Da) and flour type. Besides the contents of β-glucan and water, the molecular size of the polysaccharide and the flour quality were important determinants of the dough’s viscoelastic behavior. Compared to BG1, the higher molecular weight β-glucan (BG2) brought about major changes on all the rheological responses of the fortified doughs. The addition of appropriate levels of β-glucans and water in the poor breadmaking cultivar (Dion) doughs could yield similar viscoelastic responses to those observed by a non-fortified good breadmaking quality flour dough (Yekora).     

The effect of lipoxygenase action on the mechanical development of wheat flour doughs

The mechanical development of dough has been followed by measurement of the relaxation time of dough samples after mixing to various levels of work input. Parallel determinations of free and bound lipid have also been made. When doughs were mixed in air, addition of full-fat, enzyme-active soya bean flour (subsequently referred to as “soya flour”) resulted in an increase in relaxation time, particularly at higher work levels. The magnitude of this improvement increased with increasing work input and was dependent on the rate of work input. Addition of soya flour also enabled a higher level of mechanical work to be introduced before dough breakdown occurred. When doughs were mixed under nitrogen, or when the soya flour was heat denatured, no change in the rheological properties compared with the respective control doughs was found. The release of bound lipid, which occurred during dough development in air in the presence of soya flour, could also be induced by adding purified lipoxygenase to the dough, together with linoleic acid as a substrate. This resulted in rheological changes similar to those observed using soya flour. However addition of enzymically pre-peroxidised lipid to doughs mixed in nitrogen was without effect on relaxation times. These findings suggest that lipid release is linked with structural changes in dough protein and provide further support for a mechanism of coupled oxidation of protein -SH groups by lipoxygenase.     

RELAXATION BEHAVIOR AND THE APPLICATION OF INTEGRAL CONSTITUTIVE EQUATIONS TO WHEAT DOUGH

The small amplitude rheological properties of doughs of different compositions were measured using a dynamic rheometer. In the first study, the effect of water, flour, and added gluten on the dynamic properties was investigated. Frequency sweep was carried out in the range of 0. 1 to 100 s⁻¹ at an applied strain of 0. 1%. Storage (G') and loss (G") moduli for each composition were recorded. The storage modulus versus frequency plots were a series of parallel curves indicating that the moisture-to-flour ratio was the most important parameter. The loss modulus versus frequency plot showed a falling-off after a frequency of 40 s⁻¹. A plot of tan δ versus frequency indicated a more elastic dough at shorter time     

EFFECTS OF MIXING SOYBEAN FLOUR DOUGHS ON THEIR DYNAMIC MODULI MEASURED IN AN ORTHOGONAL RHEOMETER

An orthogonal rheometer was used to determine the dynamic loss moduli and storage moduli of defatted soybean flour doughs. Doughs of 30 to 50% moisture were prepared by mixing for 1 to 10 min in a Brabender laboratory mixing head. Both moduli decreased as dough moisture increased. Moduli increased as mixing time increased. Moduli were initially constant as percent strain increased. After 1 1/2% strain, moduli decreased with increasing strain. No effect of resting time was found.     

THE RHEOLOGICAL BASIS OF DOUGH STICKINESS

Stickiness in wheat flour doughs was studied as a function of rheological and surface properties. Adhesion was measured using a modified peel test at various water additions, peel rates and peel layer thicknesses. Peeling energy gave a strong positive correlation with subjective bakery stickiness ratings. Peeling forces were highly rate dependent and showed transitions from sticky to nonsticky behvaiour with increasing rate of peeling. Dynamic storage modulus showed a negative correlation with stickiness ratings, suggesting stickiness is primarily a rheologically controlled process. Stress relaxation gradients were greater for sticky doughs and were very similar to peeling force vs. rate slopes, again indicating that adhesion is principally a function of the rheological properties of the dough. Surface tension measurements of sticky and nonsticky dough/liquor interfaces showed no significant differences and are typical of protein solutions. Calculated values of the interfacial surface energy between dough and interface were about 100 mJ/m², typical of secondary bonding interactions such as polar or van der Waals bonding.     

Flour Quality effects on percolation of gas bubbles in wheat flour doughs

To investigate how flour affects crumb structures, we used Synchrotron X-ray tomography to scan and capture the growth of gas bubbles in leavened and unleavened bread doughs of two different flours. Bubbles were mobile in all doughs; they coalesced and disproportionated with the rate of coalescence being higher in leavened doughs. In unleavened dough, new bubbles were detected, attributed to arise from poro-visco-elastic relaxation of gluten as dough rested. In each yeasted dough, a single, massively inter-connected cluster formed which percolated at ~26% dough porosity irrespective of flour type in dough. Following percolation, dough expansion was driven primarily by growth of the percolating bubble. Between flours, the rate of coalescence was higher in Wylkatchem (Wylk), an Australian flour dough, than in Canadian Western Red Spring (CWRS) flour dough, known for superior proof and bake qualities. How the physical and rheological properties of dough liquors could have affected the stabilities of bubbles in these doughs have been discussed.     

黍子粉对小麦面团流变特性和面条品质的影响

为探究黍子粉对小麦面团粉质特性、黏弹特性和面条品质的影响以及面团流变学特性与面条品质之间的相关性分析。在小麦粉中添加0%、10%、20%、30%、40%比例的黍子粉,测定不同黍子粉添加量面团的流变学特性和面条的蒸煮特性、质构特性。结果表明:面团的弱化度、弹性模量和黏性模量,面条的断条率和蒸煮损失率随着黍子粉添加量的增加呈上升趋势;而面团的吸水率、稳定时间和形成时间,面条的粘聚性、回复性、拉伸力和拉伸距离则呈下降趋势。当黍子粉添加量为20%时,面条的硬度、胶着度、咀嚼性分别为777.8 g、637.7、588.06,与其他黍子粉添加量相比均达到最优。黍子小麦混合粉面团的弹性模量、黏性模量和弱化度与面条的拉伸力、拉伸距离等呈负相关,证明了黍子粉在面条品质定向改变方面的应用潜力。     

稻米-高筋小麦混合粉面团的静态和动态流变学特性

本实验研究了6 种稻米-高筋混合粉面团的静态和动态流变学特性。结果表明,随着稻米粉添加量的增加,在动态流变频率扫描中,混合面团的储存模量（G’）和损耗模量（G’’）整体呈上升趋势,损耗角正切（tan δ）整体呈规律性下降趋势;在静态流变的蠕变松弛实验中,最大蠕变应变量、最大蠕变柔量和瞬时恢复柔量逐渐减小,零剪切黏度和瞬间恢复比率逐渐增加,表明加入稻米粉后面筋蛋白有所稀释,但稻米粉中的淀粉吸水膨胀相互黏附,且与米蛋白相互作用赋予面团更高的弹性模量和更复杂的内部结构。在动态流变的温度扫描中,糊化温度前后混合面团的黏弹性变化明显,G’和G’’曲线类似淀粉糊化曲线,说明生面团加热的过程中,影响其流变学特性的主要成分为淀粉;降温过程中,稻米粉添加量的影响较小,高温区G’和G’’呈规律性增加,温度低于60 ℃后各项流变指标规律性差;在整个降温的过程中样品均具有类固体的性质。     

Influence of acidification on dough viscoelasticity of gluten-free rice starch-based dough matrices enriched with exogenous protein

The impact of acid incorporation (acetic + lactic, 0.5%) into rice starch-based doughs enriched with different proteins (egg albumin, calcium caseinate, pea protein and soy protein isolates) at different doses (0, 5 and 10%) has been investigated on dough viscoelastic and pasting profiles. Oscillatory (stress and frequency sweeps) and creep-recovery tests were used to characterize the fundamental viscoelastic behaviour of the doughs, and thermomechanical assays were performed to assess dough viscometric performance. Supplementation of gluten-free doughs with proteins from vegetal sources led to more structured dough matrices (higher viscoelastic moduli and steady viscosities, and lower tan δ, instantaneous and retarded elastic compliances) effect being magnified with protein dose. Acid addition decreased these effects. Incorporation of proteins from animal source resulted in different viscoelastic behaviours according to the protein type, dosage and acidification, especially for casein. Acidification conferred lower dough deformation and notably higher steady viscosity and viscoelastic moduli for 5 %-casein-added dough. Protein-acid interaction favoured higher viscosity profiles, particularly for doughs with proteins of vegetable origin and lower dosage. Dough acidification decreased the pasting temperatures and the amylose retrogradation. Acidification of protein-enriched rice-starch doughs allowed manipulation of its viscometric and rheological properties which is of relevant importance in gluten-free bread development.     

Rheological and physical evaluation of jet‐cooked oat bran in low calorie cookies†

Different levels of shortening in cookies (10%, 20% and 30% by weight) were replaced with 20% jet‐cooked oat bran, also called Nutrim oat bran (OB), to prepare cookies with fewer calories. The cookies containing Nutrim OB were investigated in terms of rheological and physical properties and compared with a control. As more shortening was replaced with Nutrim OB, a decrease in the diameter and an increase in the height of cookies were observed. The increased moisture content from Nutrim OB caused a decrease in the dynamic viscoelastic properties of cookie dough. Squeezing flow method showed shear thinning behaviours in all cookie doughs. Also, the elongational viscosity of cookie dough decreased significantly with more replacement of shortening with Nutrim OB. The rheological properties of cookie dough during baking indicated that all of the samples had similar types of viscoelastic characteristics during baking. However, the cookies containing more Nutrim OB exhibited more elastic properties which resulted in a decreased cookie diameter. There was no significant difference in cookie hardness among samples with up to 20% shortening replacement but the cookies became lighter in colour as the Nutrim OB content was increased.     

Effects of transglutaminase on the rheological and Mixolab thermomechanical characteristics of oat dough

The effects of added transglutaminase (TG) on the rheological and thermal properties of oat dough were evaluated. Mixolab, rheometer, and differential scanning calorimetry (DSC) were used to analyse the oat dough for changes in thermomechanical, rheological, and thermal properties. TG had distinct effects on dough water absorption, modified viscoelastic behaviour, and enhanced thermal stability. The dough also exhibited a decrease in the number of free amino groups after TG treatment, confirming protein cross-linking catalysed by TG. Electrophoresis of TG-treated oat protein fractions using SDS–PAGE, which was also used to analyse the effects of TG on the protein fractions of oat flour; it showed that both globulin and avenin were good substrates for TG.