Underwater digital holography for studies of marine plankton

Conventional and digital holographies are proving to be increasingly important for studies of marine zooplankton and other underwater biological applications. This paper reports on the use of a subsea digital holographic camera (eHoloCam) for the analysis and identification of marine organisms and other subsea particles. Unlike recording on a photographic film, a digital hologram (e-hologram) is recorded on an electronic sensor and reconstructed numerically in a computer by simulating the propagation of the optical field in space. By comparison with other imaging techniques, an e-hologram has several advantages such as three-dimensional spatial reconstruction, non-intrusive and non-destructive interrogation of the recording sampling volume and the ability to record holographic videos. The basis of much work in optics lies in Maxwell's electromagnetic theory and holography is no exception: we report here on two of the numerical reconstruction algorithms we have used to reconstruct holograms obtained using eHoloCam and how their starting point lies in Maxwell's equations. Derivation of the angular spectrum algorithm for plane waves is provided as an exact method for the in-line numerical reconstruction of digital holograms. The Fresnel numerical reconstruction algorithm is derived from the angular spectrum method. In-line holograms are numerically processed before and after reconstruction to remove periodic noise from captured images and to increase image contrast. The ability of the Fresnel integration reconstruction algorithm to extend the reconstructed volume beyond the recording sensor dimensions is also shown with a 50% extension of the reconstruction area. Finally, we present some images obtained from recent deployments of eHoloCam in the North Sea and Faeroes Channel.     

Digital Double-pulsed Holographic Interferometry for Vibration Analysis

Abstract

In this paper we describe a digital double-pulsed holographic system. Two separate holograms of an object under test are recorded within a few microseconds using a charge-coupled device camera and stored in a frame grabber. The holograms are digitally reconstructed using a computer, by simulation of the Fresnel diffraction of the hologram illuminated by the reference wave. The phases of the two reconstructed wave fields are calculated from the complex amplitude and the deformation is obtained from the phase difference. Experimental results are presented.     

Fourier-domain digital holographic optical coherence imaging of living tissue

Digital holographic optical coherence imaging is a full-frame coherence-gated imaging approach that uses a CCD camera to record and reconstruct digital holograms from living tissue. Recording digital holograms at the optical Fourier plane has advantages for diffuse targets compared with Fresnel off-axis digital holography. A digital hologram captured at the Fourier plane requires only a 2D fast Fourier transform for numerical reconstruction. We have applied this technique for the depth-resolved imaging of rat osteogenic tumor multicellular spheroids and acquired cross-section images of the anterior segment and the retinal region of a mouse eye. A penetration depth of 1.4 mm for the tumor spheroids was achieved.     

Three-dimensional Displacement Analysis by Windowed Phase-shifting Digital Holographic Interferometry

Abstract:  Phase-shifting digital holography is a new method for measuring the displacement distribution on the surface of an object. The authors previously proposed a windowed phase-shifting digital holographic interferometry (windowed PSDHI). This method provides accurate displacement distributions by decreasing the effect of speckle patterns. In this study, the method is extended to analyse three-dimensional displacement components in a microscope. Three object laser beams in the optical system are used. Four phase-shifted holograms are recorded for each object laser beam. The complex amplitude of each reconstructed light at the object is calculated by the Fresnel diffraction integral of the complex amplitude of the hologram. The reconstructed distance is obtained at the point with the maximum of the standard deviation of the intensities of the object reconstructed with changing the reconstruction distance. The three phase-difference values between before and after deformation provide the three-dimensional displacement components. Theoretical treatment and experimental results of three-dimensional displacement measurement using this method are shown.     

Digital recording and reconstruction of holograms in hologram interferometry and shearography

The fundamentals of digital recording and mathematical reconstruction of Fresnel holograms are described. The object is recorded in two different states, and the holograms are stored electronically with a charge-coupled-device detector. In the process of reconstruction the digitally sampled holograms are applied to the different coherent optical methods as hologram interferometry and shearography. If the holograms are superimposed and reconstructed jointly, a holographic interferogram results. If a shearing is introduced in the reconstruction process, a shearogram results. This means that the evaluation technique, e.g., hologram interferometry or shearography, can be influenced by numerical methods.     

Reconstruction of partially occluded objects encoded in three-dimensional scenes by using digital holograms

We propose a task-specific digital holographic capture system for three-dimensional scenes, which can reduce the amount of data sent from the camera system to the receiver and can effectively reconstruct partially occluded objects. The system requires knowledge of the object of interest, but it does not require a priori knowledge of either the occlusion or the distance the object is from the camera. Subwindows of the camera-plane Fresnel field are digitally propagated to reveal different perspectives of the scene, and these are combined to overcome the unknown foreground occlusions. The nature of the occlusions and the effect of subwindows are analyzed thoroughly by using the Wigner distribution function. We demonstrate that a careful combination of reconstructions from subwindows can reveal features that are not apparent in a reconstruction from the whole hologram. We provide results by using optically captured digital holograms of real-world objects and simulated occlusions.     

Multiple fractional fourier transform hologram by use of holographic lens

Abstract

A new method is presented to record multiple fractional Fourier transform holograms by use of a holographic lens. With holographic lenses, several fractional Fourier transform holograms of different objects can be recorded in a simple way, and images of these recorded objects can be reconstructed in different positions and directions in three-dimensional space. In this paper, the theory and characteristics of the hologram recorded using the holographic lens are analysed. Using this method, a multiple fractional Fourier transform hologram was fabricated, with satisfactory results.     

Parameter-optimized digital holographic microscope for high-resolution living-cell analysis

A parameter-optimized off-axis setup for digital holographic microscopy is presented for simultaneous, high-resolution, full-field quantitative amplitude and quantitative phase-contrast microscopy and the detection of changes in optical path length in transparent objects, such as undyed living cells. Numerical reconstruction with the described nondiffractive reconstruction method, which suppresses the zero order and the twin image, requires a mathematical model of the phase-difference distribution between the object wave and the reference wave in the hologram plane. Therefore an automated algorithm is explained that determines the parameters of the mathematical model by carrying out the discrete Fresnel transform. Furthermore the relationship between the axial position of the object and the reconstruction distance, which is required for optimization of the lateral resolution of the holographic images, is derived. The lateral and the axial resolutions of the system are discussed and quantified by application to technical objects and to living cells.     

Point-spread function synthesis in scanning holographic microscopy

Scanning holographic microscopy is a two-pupil synthesis method allowing the capture of single-sideband in-line holograms of noncoherent (e.g., fluorescent) three-dimensional specimens in a single two-dimensional scan. The flexibility offered by the two-pupil method in synthesizing unusual point-spread functions is discussed. We illustrate and compare three examples of holographic recording, using computer simulations. The first example is the classical hologram in which each object point is encoded as a spherical wave. The second example uses pupils with spherical phase distributions having opposite curvatures, leading to reconstructed images with a resolution limit that is half that of the objective. In the third example, axicon pupils are used to obtain axially sectioned images.     

调频加网对于全息再现图像的影响

目的 对全息图进行加网处理,实现计算全息图的二值化,将计算全息图应用于印刷领域。方法 本文设计计算全息图进行调频加网的整体方案,讨论不同的加网算法对计算全息再现图像的质量影响。首先,对3幅不同类型的灰度图片进行计算全息编码得到全息图;然后利用误差扩散算法和抖动算法对全息图进行调频加网获得二值化全息图;之后通过光场重建得到全息再现图像。结果 对全息再现图像进行峰值信噪比和结构相似性数据比较发现,误差扩散算法更适用于计算全息二值化处理,抖动加网使计算全息图产生周期性图案,导致再现全息图产生混频现象,全息再现图像的质量下降。结论 加网导致全息图再现质量下降,误差扩散算法可以得到较好的再现像,适用于全息图的二值化处理;与此同时,抖动算法会产生混频现象,因此抖动算法并不适用于全息图二值化处理。     

Low-bit-rate computer-generated color Fresnel holography with compression ratio of over 1600 times using vector quantization [Invited

We propose a method for compressing a digital color Fresnel hologram based on vector quantization (VQ). The complex color hologram is first separated into three complex holograms, each representing one of the primary colors. Subsequently, each hologram is converted into what we call a real Fresnel hologram and compressed with VQ based on a universal codebook. Experimental evaluation reveals that our scheme is capable of attaining a compression ratio of over 1600 times and still preserving acceptable visual quality on the reconstructed images. Moreover, the decoding process is free from computation and highly resistant to noise contamination on the compressed data.     

Magnified reconstruction of digitally recorded holograms by Fresnel-Bluestein transform

A method for numerical reconstruction of digitally recorded holograms with variable magnification is presented. The proposed strategy allows for smaller, equal, or larger magnification than that achieved with Fresnel transform by introducing the Bluestein substitution into the Fresnel kernel. The magnification is obtained independent of distance, wavelength, and number of pixels, which enables the method to be applied in color digital holography and metrological applications. The approach is supported by experimental and simulation results in digital holography of objects of comparable dimensions with the recording device and in the reconstruction of holograms from digital in-line holographic microscopy.     

Recognition and classification of three-dimensional phase objects by digital Fresnel holography

We demonstrate the validity of wavelet-based processing for recognition and classification of three-dimensional phase objects. One Fresnel digital hologram of each of the three-dimensional (3-D) phase objects to be classified is recorded. The electronic holograms are processed digitally to permit 3-D object information to be retrieved as two-dimensional digital complex images. We use a Mexican-hat wavelet- matched filter (WMF) to enhance the correlation peak and discriminate between the objects. The WMF performs a wavelet transform (WT) to enhance the significant features of the images and the correlation of the WT coefficients thus obtained. We compare the feasibility of a WMF-based object classifier with the matched-filter-based classifier to classify our four 3-D phase objects in a 3-D scene into true or false classes with minimal error.     

Quantitative phase microscopy using dual-plane in-line digital holography

We present detailed theoretical evaluation and thorough experimental investigation of quantitative phase imaging using our previously demonstrated dual-plane in-line digital holographic microscopy technique [Opt. Lett. 35, 3426 (2010)]. This evaluation is based on the recording of two interferograms at slightly different planes and numerically reconstructing the object information. The zero-order diffracted wave is eliminated by using the method of subtraction of average intensity of the entire hologram, and the twin-image diffracted wave is removed by Fourier domain processing of the two recorded holograms. Experiments are performed using controlled amplitude and phase objects and human muscle cells to demonstrate the potential of this technique.     

Estimation of wavelength difference using scale adjustment in two-wavelength digital holographic interferometry

We propose a method for an estimation of wavelength difference using scale adjustment in two-wavelength digital holographic interferometry. To estimate wavelength difference, two holograms recorded with different wavelengths are reconstructed on the basis of the Fresnel diffraction integral, and pixel sizes in the reconstruction plane, which depend on the wavelength in recording hologram, are analyzed. In the analysis, a zero-padding method and an intensity correlation function are used to adjust pixel sizes in the reconstruction plane and then obtain a wavelength difference given by a difference between the pixel sizes. Theoretical predictions and experimental results are shown to indicate the usefulness of the proposed method in this paper.     

Digital holographic particle validation via complex wave

The inability to distinguish between particle images and noise in holographic reconstruction of dense particle fields hampers the advancement of holographic particle diagnostic techniques including holographic particle image velocimetry. We developed a method to separate particles from the noise by unlocking a unique particle signature in the complex reconstructed field. This complex-wave signature is present in digital particle holograms recorded at any scattering angle. Simulations of single and multiple particle holograms, as well as preliminary laboratory particle-field experiments, not only demonstrated the existence of the particle signature but also evaluated its ability to remove noise. Regardless of particle seeding density, scattering angle of hologram recording and particle size range, the particle identification/validation routine consistently provides >50% removal of "bad" particles and <8% of good particles.     

Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms

We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.     

Digitized holography: modern holography for 3D imaging of virtual and real objects

Recent developments in computer algorithms, image sensors, and microfabrication technologies make it possible to digitize the whole process of classical holography. This technique, referred to as digitized holography, allows us to create fine spatial three-dimensional (3D) images composed of virtual and real objects. In the technique, the wave field of real objects is captured in a wide area and at very high resolution using the technique of synthetic aperture digital holography. The captured field is incorporated in virtual 3D scenes including two-dimensional digital images and 3D polygon mesh objects. The synthetic field is optically reconstructed using the technique of computer-generated holograms. The reconstructed 3D images present all depth cues like classical holograms but are digitally editable, archivable, and transmittable unlike classical holograms. The synthetic hologram printed by a laser lithography system has a wide viewing zone in full-parallax and give viewers a strong sensation of depth, which has never been achieved by conventional 3D systems. A real hologram as well as the details of the technique is presented to verify the proposed technique.     

Effects of quantization in phase-shifting digital holography

We discuss quantization effects of hologram recording on the quality of reconstructed images in phase-shifting digital holography. We vary bit depths of phase-shifted holograms in both numerical simulation and experiments and then derived the complex amplitude, which is subjected to Fresnel transformation for the image reconstruction. The influence of bit-depth limitation in quantization has been demonstrated in a numerical simulation for spot-array patterns with linearly varying intensities and a continuous intensity object. The objects are provided with uniform and random phase modulation. In experiments, digital holograms are originally recorded at 8 bits and the bit depths are changed to deliver holograms at bit depths of 1 to 8 bits for the image reconstruction. The quality of the reconstructed images has been evaluated for the different quantization levels.     

Multiple-viewpoint projection holograms synthesized by spatially incoherent correlation with broadband functions

We present a theoretical framework for recording and reconstructing incoherent correlation holograms of real-existing three-dimensional scenes observed from multiple viewpoints. This framework is demonstrated by generating and reconstructing a modified Fresnel hologram as well as a new correlation hologram called a protected correlation hologram. The reconstructed scene obtained from the protected correlation hologram has a significantly improved transverse resolution for the far objects in the scene compared to the modified Fresnel hologram. Additionally, the three-dimensional information encoded into the protected correlation hologram is scrambled by a secretive point spread function and thus the hologram can be used for encrypting the observed scene. The proposed holography methods are demonstrated by both simulations and experiments.