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CREB binding protein is a coactivator for the androgen receptor and mediates cross-talk with AP-1 总被引:1,自引:0,他引:1
K Fr?nsdal N Engedal T Slagsvold F Saatcioglu 《Canadian Metallurgical Quarterly》1998,273(48):31853-31859
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CT Powell NJ Brittis D Stec H Hug WD Heston WR Fair 《Canadian Metallurgical Quarterly》1996,7(4):419-428
Others have reported that the phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA), an activator and down-regulator of most protein kinase C (PKC) isozymes, can induce apoptotic cell death of androgen-sensitive LNCaP but not androgen-insensitive PC-3 or DU 145 human prostate cancer cells. As a first step toward uncovering the mechanism by which TPA induces apoptosis of LNCaP cells, we quantified expression of PKC isozyme mRNAs in unmodified and TPA-resistant LNCaP cells and in naturally TPA-resistant PC-3, PC-3M, and DU 145 cells. All of the cell lines and normal prostate expressed RNAs for PKC alpha, delta, epsilon, eta, and mu; only DU 145 cells and normal prostate expressed PKC beta and theta RNAs, and none expressed PKC gamma. The amount of PKC alpha RNA and protein was 6- to 38-fold lower, and PKC mu RNA was 4.5- to 16.5-fold higher in unmodified and TPA-resistant LNCaP cells than in the androgen-independent cells. We examined the effects of TPA on PKC alpha and mu mRNA levels and on membrane translocation of PKC alpha. Incubation with TPA for 6 h or more induced 95% inhibition of cell growth, a transient 12-fold increase and 5-fold decrease in PKC alpha and mu mRNA levels, respectively, and prolonged translocation of PKC alpha to non-nuclear membranes in unmodified LNCaP cells and in TPA-resistant LNCaP cells from which TPA had been removed for 10 days. TPA-resistant LNCaP cells in the continuous presence of TPA, or 24 h after removal of TPA, had down-regulated PKC alpha and remained resistant to re-addition of TPA. These data demonstrate a strong correlation of the presence and absence of membrane PKC alpha with apoptosis and resistance to apoptosis, respectively. 相似文献
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SA Onate V Boonyaratanakornkit TE Spencer SY Tsai MJ Tsai DP Edwards BW O'Malley 《Canadian Metallurgical Quarterly》1998,273(20):12101-12108
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W Feng RC Ribeiro RL Wagner H Nguyen JW Apriletti RJ Fletterick JD Baxter PJ Kushner BL West 《Canadian Metallurgical Quarterly》1998,280(5370):1747-1749
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Steroid receptor coactivator-1 is a histone acetyltransferase 总被引:2,自引:0,他引:2
TE Spencer G Jenster MM Burcin CD Allis J Zhou CA Mizzen NJ McKenna SA Onate SY Tsai MJ Tsai BW O'Malley 《Canadian Metallurgical Quarterly》1997,389(6647):194-198
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