共查询到19条相似文献，搜索用时 62 毫秒
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利用MEMS技术小批量加工了薄膜金电极。采用电化学沉积法在金电极表面修饰纳米铂黑颗粒,以有机功能性材料二茂铁作为电子媒介体,通过戊二醛-牛血清白蛋白共价交联固定葡萄糖氧化酶制得葡萄糖生物传感器。考察了不同修饰电极的电化学行为以及酶固定量和戊二醛浓度对传感器响应特性的影响。实验结果表明:该传感器响应时间仅为5s,在0.29V的低工作电压下,线性测量范围可达到0.5～22mmol/L,灵敏度为50.35μA/(cm2.mmol.L-1),相关系数为0.9925,差异系数为4.28%。 相似文献
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目前,发酵工业因缺少高效在线检测手段,生产过程中不能及时跟踪底物或产物浓度变化,导致发酵效率低,污染排放严重。电化学酶生物传感器能够实现对生化反应中特定组分浓度的快速精确检测,已广泛应用于医疗行业。然而,该传感器检测范围过窄,在真实发酵体系中,需预先对样品进行稀释处理,仅能实现离线检测。为此,提出一种超宽范围的新型电化学酶生物传感器及检测方法。传感器采用三电极丝网印刷结构,工作电极为自制的规则普鲁士蓝纳米颗粒传感膜及生物酶,检测采用流动注射分析技术。测试表明,该传感器及检测系统对葡萄糖浓度的检测范围为0.5-120g/L,测量误差在2 %以内,测量周期< 60秒,完全满足发酵工业在线检测要求。 相似文献
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生物恐怖袭击和生物传感器 总被引：5，自引：0，他引：5
<正> 2001年最后的三四个月,美国纽约和华盛顿等地屡遭恐怖袭击。这些袭击可分为两类,第一类是“9.11”事件:国际恐怖分子劫持商业航班的飞机作武器,对纽约世界贸易中心两座姐妹摩天大楼和华盛顿的五角大楼进行突然袭击,在一二个小时内就夺去了四千多人的宝贵生命,造成难以估算的损失,给世界经济蒙上了浓浓的阴影,对世界的未来将会产生深远的影响。 第二类恐怖袭击是炭疽孢子袭击。“9.11”事件后的几个月内,纽约、华盛顿等城市不断发现带有炭疽孢子的邮件,一些邮局和几大电视台网的雇员遭到炭疽热的致命袭击,闹得人心惶惶。据说为了应付炭疽袭击,两个月内洛杉矶市就已用去约1400万美元。 相似文献
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用于侵入性及非侵入性患者监视的固态集成传感器已逐渐可行并令人瞩目,因为这种传感器尺寸缩小,更可靠并结合了单片信号处理.对于许多生物和生理研究程序及一些临床应用,患者的侵入性监视已成为重要的焦点.这些应用包括很广的领域,从物理和化学生物事件的简单测量,如记录单个神经细胞内电化学产生生物势的放电,到增强或恢复障碍功能的全 相似文献
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Shunsheng Cao Long Fang Zhiyuan Zhao Yi Ge Sergey Piletsky Anthony P. F. Turner 《Advanced functional materials》2013,23(17):2102-2102
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Shunsheng Cao Long Fang Zhiyuan Zhao Yi Ge Sergey Piletsky Anthony P. F. Turner 《Advanced functional materials》2013,23(17):2162-2167
In this work, the first example of a hierarchically structured hollow silica system is reported without any chemical modification to the enzyme involved in the process. The leaching of the physically adsorbed enzyme is substantially restrained in comparison to pure hollow silica supports. The hierarchical architecture is composed of the ordered hollow silica spheres with a shell‐in‐shell structure. This rationally integrated architecture, which serves as the host for glucose oxidase immobilization, displays many significant advantages, including increases in mechanical stability, enzyme loading, and bioactivity, and a decrease in enzyme leaching compared to existing pure hollow silica matrices. This facilitates further multifarious applications for enhanced enzyme immobilization, biosensors, and biocatalysis. 相似文献
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目前AlGaN/GaN高电子迁移率晶体管(HEMT)生物传感器表面修饰及分子识别元件固定需在金属或氧化物门电极上进行,针对金属或氧化物门电极的存在增加了器件的制作难度,提高了制作成本,同时还影响了传感器灵敏度的提高等问题,提出了进行"生物分子膜"门电极的研究。传感器的表面采用3-氨基丙基三乙氧基硅烷(APTES)进行修饰作为分子识别元件固定的基底,该方法在降低传感器的制作成本的同时,提高了传感器的灵敏度,传感区域为长度L=5μm、宽度W=160μm的传感器测量质量浓度为0.1ng/mL的山羊免疫球蛋白(IgG)时,传感器源漏间电流呈现约30μA的电流降。该传感器反应速度快,适用于实时监测,在环境监控、医疗等领域有着良好的应用前景。 相似文献
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Chao Fang Zheng Deng Guodong Cao Qiang Chu Yulian Wu Xiang Li Xinsheng Peng Gaorong Han 《Advanced functional materials》2020,30(16)
Chemodynamic therapy (CDT), enabling selective therapeutic effects and low side effect, attracts increasing attention in recent years. However, limited intracellular content of H2O2 and acid at the tumor site restrains the lasting Fenton reaction and thus the anticancer efficacy of CDT. Herein, a nanoscale Co–ferrocene metal–organic framework (Co‐Fc NMOF) with high Fenton activity is synthesized and combined with glucose oxidase (GOx) to construct a cascade enzymatic/Fenton catalytic platform (Co‐Fc@GOx) for enhanced tumor treatment. In this system, Co‐Fc NMOF not only acts as a versatile and effective delivery cargo of GOx molecules to modulate the reaction conditions, but also possesses excellent Fenton effect for the generation of highly toxic ?OH. In the tumor microenvironment, GOx delivered by Co‐Fc NMOF catalyzes endogenous glucose to gluconic acid and H2O2. The intracellular acidity and the on‐site content of H2O2 are consequently promoted, which in turn favors the Fenton reaction of Co‐Fc NMOF and enhances the generation of reactive oxygen species (ROS). Both in vitro and in vivo results demonstrate that this cascade enzymatic/Fenton catalytic reaction triggered by Co‐Fc@GOx nanozyme enables remarkable anticancer properties. 相似文献
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DALUWATHU MULLA GAMAGE PREETHICHANDRA MITSUYOSHI ONODA 《Electronics and Communications in Japan》2016,99(7):55-63
In order to improve biosensor performance, namely, the sensitivity, response time, and linear range values, novel amperometric polypyrrole (PPy) glucose biosensors, achieved by three different immobilizing techniques, namely, physical adsorption, coentrapment, and a two‐step method of coentrapment with superimposed physical adsorption, have been studied. Different enzyme loading and material properties resulted in varied immobilizing techniques, leading to dissimilarities in characteristics. In this study, Pt coated AnodiscsTM with 0.2 μm maximum pore size were used as electrodes to polymerize PPy at 0.3 mAcm−2 using a solution containing 0.05 mol/L pyrrole and 0.1 M NaPF6. The polymerization time was optimized to 90 s. In the coentrapment method, glucose oxidase (GOx) (1 mg/mL) was added to the monomer solution while an aliquot of GOx (5 μL) was placed on the prepolymerized electrode for physical adsorption. A combination of these two techniques was used as the last immobilization technique. The physical adsorption method gave a sensitivity of 3 mAcm−2 (mol/L)−1 and a linear range of 0.5 × 10−3 mol/L to 13 × 10−3 mol/L with a response time of 3 s. The sensitivity in the case of coentrapment was 3.75 mAcm−2 (mol/L)−1, while the value was 4.45 mAcm−2 (mol/L)−1 in two‐step immobilization, thus giving 25% and 48% increases, respectively. The response times of 9 s and 8 s in the latter cases reveal possible repercussion taking place in enzyme immobilization in a three‐dimensional (3D) PPy matrix and a delay in the time required for glucose to reach the enzyme. The linear range of the two‐step method was extended to 16 × 10−3 mol/L by reinforced enzyme loading. 相似文献
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Kyle Benson Ananta Ghimire Ajith Pattammattel Challa V. Kumar 《Advanced functional materials》2017,27(43)
A highly efficient, multifunctional, bioderived white‐emitting hydrogel (biophosphor) consisting of crosslinked bovine serum albumin and three fluorescent dyes, Coumarin 460, fluorescein, and 5(6)‐carboxy‐x‐rhodamine, is reported here. White emission is obtained upon excitation of the biophosphor at 365 nm with appropriate mole ratios of the above dyes. The CIE 1931 chromaticity coordinates of white emission with 365 nm excitation are (0.36, 0.37), and the correlated color temperature is 5300 K. Multifunctional nature of the biophosphor is also demonstrated. A UV‐light‐emitting‐diode (361 nm) coated with this biophosphor, for example, indicates white emission (CIE 0.28, 0.31) with a half‐life of 106 (±5) h. The white emission is also highly sensitive to pH over a broad range (pH 1–11). Incorporation of glucose oxidase and peroxidase in the biophosphor allows for the detection of glucose over a physiologically relevant range of 1.8–288 mg dL?1. This is a unique, advanced biophosphor with LED and sensing applications, and it is the first example of a multifunctional, proteinaceous white emitter. 相似文献