Application of magnetic iron oxide nanoparticles prepared from microemulsions for protein purification

BACKGROUND: Magnetic nanoparticles are of immense interest for their applications in biotechnology. This paper reports the synthesis of magnetic iron oxide nanoparticles from two different water‐in‐oil microemulsion systems (ME‐MIONs), their characterization and also their use in purification of coagulant protein. RESULTS: ME‐MIONs have demonstrated to be an efficient binder in the purification of Moringa oleifera protein when compared with the superparamagnetic iron oxide nanoparticles prepared from coprecipitation in aqueous media. The size and morphology of the ME‐MIONs were studied by transmission electron microscopy (TEM) while the structural characteristics were studied by X‐ray diffraction (XRD). The microemulsion magnetic iron oxide nanoparticles (ME 1‐MION and ME 2‐MION) obtained have a size range 7–10 nm. The protein and ME‐MIONs interaction was investigated by Fourier transform infrared spectroscopy (FT‐IR); the presence of three peaks at 2970, 2910 and 2870 cm^?1 respectively, confirms the binding of the protein. The purification and molecular weight of the coagulant protein was 6.5 kDa as analyzed by SDS‐PAGE. CONCLUSION: The ME‐MIONs have the advantage of being easily tailored in size, are highly efficient as well as magnetic, cost effective and versatile; they are, thus, very suitable for use in a novel purification technique for protein or biomolecules that possess similar characteristics to the Moringa oleifera coagulant protein. Copyright © 2011 Society of Chemical Industry     

Azo dye removal by Moringa oleifera seed extract coagulation

In this paper, the ability of Moringa oleifera seed extract–among other natural coagulants–to remove several different types of dyes has been researched. Moringa oleifera has been demonstrated to have a high removal ability for anionic dyes. This study is particularly focused on testing the removal of an azo dye such as Chicago Sky Blue 6B (CSB). It has taken out the fast kinetic of coagulant action and the high potential of this coagulant agent to treat wastewater from dyestuff. Up to 99% of CSB removal has been achieved with M. oleifera extract dosage of 150, 200 and 250 mg l^?1 for 100, 160 and 200 mg l^?1 of initial CSB concentration, respectively. Temperature does not affect the coagulant process and a pH level greater than 8 has a negative influence. Lower CSB percentage removal is achieved by increasing the initial dye concentration, but an optimum relationship between dye amount removed and M. oleifera extract amount has been established and it appears to be between 0.7 and 0.9, depending on the initial dye concentration and the bulk of the remaining dye concentration.     

Biosorption of Cu(II) onto agricultural materials from tropical regions

BACKGROUND: In Ghana, the discharge of untreated gold mine wastewater contaminates the aquatic systems with heavy metals such as copper (Cu), threatening ecosystem and human health. The undesirable effects of these pollutants can be avoided by treatment of the mining wastewater prior to discharge. In this work, the sorption properties of agricultural materials, namely coconut shell, coconut husk, sawdust and Moringa oleifera seeds for Cu(II) were investigated. RESULTS: The Freundlich isotherm model described the Cu(II) removal by coconut husk (R² = 0.999) and sawdust (R² = 0.993) very well and the Cu(II) removal by Moringa oleifera seeds (R² = 0.960) well. The model only reasonably described the Cu(II) removal by coconut shell (R² = 0.932). A maximum Cu(II) uptake of 53.9 mg g^?1 was achieved using the coconut shell. The sorption of Cu(II) onto coconut shell followed pseudo‐second‐order kinetics (R² = 0.997). FTIR spectroscopy indicated the presence of functional groups in the biosorbents, some of which were involved in the sorption process. SEM‐EDX analysis confirmed an exchange of Mg(II) and K(I) for Cu(II) on Moringa oleifera seeds and K(I) for Cu(II) on coconut shell. CONCLUSION: This study shows that coconut shell can be an important low‐cost biosorbent for Cu(II) removal. The results indicate that ion exchange, precipitation and electrostatic forces were involved in the Cu(II) removal by the biosorbents investigated. Copyright © 2011 Society of Chemical Industry     

Experimental Modeling and Simulation of Supercritical Fluid Extraction of Moringa oleifera Seed Oil by Carbon Dioxide

Moringa oleifera seed is an important source of high oleic acid in vegetable oil. In the present work, supercritical extraction of Moringa seed oil has been carried out to study the influence of operating temperature, pressure, particle size, carbon dioxide flow rate, and co-solvent addition by performing experiments in the range of 333–373?K, 20–40?MPa, 0.50–1.00?mm, 0.83?×?10^?4–2.50?×?10^?4?kg/s, and 0–10% ethanol. The extraction data have been successfully modeled by extended Lack’s model and fitting parameters are optimized by the Box global optimization technique. The results showed that pressure has a significant effect followed by temperature, co-solvent, solvent flow rate, and particle size.     

Casein based biogenic‐synthesized zinc oxide nanoparticles simultaneously decontaminate heavy metals,dyes, and pathogenic microbes: a rational strategy for wastewater treatment

BACKGROUND

Wastewater management has become a great concern recently due to industrial and domestic discharges containing metals, organic pollutants and pathogenic microbes that contaminate water bodies and also cause waterborne diseases. Although there have been multiple conventional processes for removing contaminants, nanomaterials have recently gained importance for wastewater treatments.

RESULTS

Here, ZnONP_CS with an average size of 10 nm was successfully synthesized by using casein as biogenic reducing and capping agent. Removal of three metals and two dyes was investigated and it was found that nearly 90% was removed. Adsorption isotherm data were best fitted with the Langmuir model, and the maximum adsorption capacity (mg g^?1) was found to be 156.74 for Cd(II), 194.93 for Pd(II), 67.93 for Co(II), 115.47 for methylene blue and 62.19 for congo red. Repeated cycles of regeneration and reuse showed a drop in removal efficiency of 10–13%. Further, nanoparticles also showed efficient photo‐degradation of dyes within 240 min. Moreover, ZnONP_CS demonstrated high antibacterial potential for Escherichia coli in the presence of metals and dyes.

CONCLUSION

Biogenic synthesized ZnONP_CS demonstrated simultaneous removal of metals, dyes as well as pathogenic microbes, and could be used as a cost‐effective and reusable system in various industrial effluent treatments. © 2018 Society of Chemical Industry     

Preparative Isolation of Bioactive Nitrile Glycoside “Niazirin” from the Fruits of Moringa oleifera Using Fast Centrifugal Partition Chromatography

Fast centrifugal partition chromatography was successfully applied in the separation of bioactive constituent niazirin directly from the chloroform extract of Moringa oleifera. The experiment was performed with a two-phase solvent system composed of ethyl acetate/BuOH/water (6:0.5:4 v/v/v) in which the upper organic layer was used as stationary phase and lower aqueous phase was used as mobile phase. From 1 g of crude extract, 70 mg of niazirin was obtained in 94.8% purity as determined by HPLC. The total yield recovery was >94%. The isolated nitrile glycoside (niazirin) was characterized on the basis of its ¹H, ¹³C-NMR and ESI-MS data.     

Biocorrosion inhibition of mild steel in crude oil-water environment using extracts of Musa paradisiaca peels,Moringa oleifera leaves,and Carica papaya peels as biocidal-green inhibitors: kinetics and adsorption studies

Biocorrosion inhibition of mild steel in the crude oil-water environment was investigated in the absence and presence of Carica papaya peel extract, Musa paradisiaca peel extract, and Moringa oleifera leaf extract as potential biocidal-green inhibitors using gravimetric, polarization, and surface analysis (Inverted metallurgical microscope and Fourier transform infra-red spectroscopy) methods. Kinetics and adsorption behaviors of the extracts were determined and evaluated. Results revealed that each of the extracts functioned as biocidal-green inhibitors in the crude oil-water environment and that biocorrosion inhibition efficiency (IE%) of each extract increased with increase in extract concentration and exposure time. Maximum IE% of 97.7, 96.5, and 99.2% was achieved with Musa paradisiaca peel extract, Moringa oleifera leaf extract, and Carica papaya peel extract, respectively, at 4000?mg/l extract concentration and 35 d exposure time. Polarization studies indicated that each of the extracts acts as a mixed-type inhibitor. Mild steel susceptibility to biocorrosion was significantly reduced by each of the extracts through biofilm development suppression and extract adsorption onto the mild steel surface which obeyed Langmuir, Freundlich, Temkin, and Flory-Huggins isotherm models. Kinetics of mild steel biocorrosion inhibition followed a Monod form of kinetics (Type A). Gibbs free energy of adsorption values for Musa paradisiaca peel extract (?9.65?kJ/mol), Moringa oleifera leaf extract (?9.57?kJ/mol), and Carica papaya peel extract (?9.71?kJ/mol) showed that each extract adsorption was spontaneous and of physical adsorption.     

A novel method of unburned hydrocarbons and NOx gases capture from vehicular exhaust using natural biosorbent

Automobile emissions are composed of NO_x and unburned hydrocarbon that contribute significantly to major environmental and health issues. In this study, encapsulated Moringa oleifera beads (EMBs) were synthesized using Moringa oleifera pod powder that was cross-linked with calcium alginate and used as a biosorbent for reducing the emission gas concentrations from the single-cylinder diesel engine. The breakthrough curve was attained from single and double stage of fixed bed column by the influence of temperature ranging from (80°C–120°C) ± 5°C with a feed flow rate varying from 8 to 10 kg hr^–1 and bed height varying from 15 to 30 cm. Based on the experimental results, the maximum biosorption capacity (q_o) was found to be 14.45 and 123.51 mg g^–1 for HC and NO_x, respectively, and was obtained at 80°C–90°C with double stage of BH–30cm under flow rate of 8 kg hr^–1. Further, breakthrough curves were investigated, and the experimental data were fitted using well-established models like Thomas, Yoon–Nelson, and Wang models. In addition, mass transfer models like Weber–Morris and Boyd were investigated to identify the rate-limiting step of the overall biosorption process.     

Fatty Acids Profile and Chemical Composition of Egyptian Moringa oleifera Seed Oils

Moringa oleifera is grown all over the world as a crop for its nutritious pods, leaves and seeds. The purpose of the present study was to evaluate the oil percentage, density, iodine value, saponification value, acid value and fatty acid profile of Egyptian moringa Seed oils. Moringa seeds were irradiated using ⁶⁰Co at dose levels of 0.0 (control), 5, 10 and 15 kGy and oil was extracted from unirradiated and irradiated samples. Results showed that the oil percentage and density were almost unaffected. Irradiation reduced the iodine value, whereas the acid and saponification values were increased in all irradiated samples. The oil was found to contain high levels of unsaturated fatty acids, especially an omega‐9‐fatty acid (oleic) (up to 76.29 %) at a dose level of 15 kGy. Also at the same dose level, the dominant saturated acids were palmitic, stearic acid and arachidic (the three up to 12.66 %). GC–MS revealed the presence of different compounds (more than 50) in the moringa oil extract, among them alkaloids, terpenoids steroids, saturated and unsaturated fatty acids, aromatic and aliphatic hydrocarbons and polyphenolic compounds. Phenolic and falvonoid were significantly increased by increasing irradiation dose levels. Also, the antioxidant activity of irradiated seed oil increased by increasing the phenolic and flavonoid contents. Moringa oleifera could be grown by wide scale production as a potentially valuable crop. However, isolation of individual compounds and their biological activities needs to be covered in future to enhance its pharmacological importance and to open new avenues of research.     

Heterogeneous catalyzed biodiesel production from Moringa oleifera oil

In this study, biodiesel was produced from Moringa oleifera oil using sulfated tin oxide enhanced with SiO₂ (SO₄²⁻/SnO₂-SiO₂) as super acid solid catalyst. The experimental design was done using design of experiment (DoE), specifically, response surface methodology based on three-variable central composite design (CCD) with alpha (α) = 2. The reaction parameters studied were reaction temperature (60 °C to 180 °C), reaction period (1 h to 3 h) and methanol to oil ratio (1:6 to 1:24). It was observed that the yield up to 84 wt.% of Moringa oleifera methyl esters can be obtained with reaction conditions of 150 °C temperature, 150 min reaction time and 1:19.5 methanol to oil ratio, while catalyst concentration and agitation speed are kept at 3 wt.% and 350-360 rpm respectively. Therefore this study presents the possibility of converting a relatively new oil feedstock, Moringa oleifera oil to biodiesel and thus reducing the world's dependency on existing edible oil as biodiesel feedstock.     

Palm oil mill effluent pretreatment using Moringa oleifera seeds as an environmentally friendly coagulant: laboratory and pilot plant studies

This research paper covers the suitability of the coagulation–flocculation process using Moringa oleifera seeds after oil extraction as a natural and environmentally friendly coagulant for palm oil mill effluent treatment. The performance of M. oleifera coagulant was studied along with the flocculant KP 9650 in removal of suspended solids, organic components and in increasing the floc size. The optimum values of the operating parameters obtained from the laboratory jar test were applied in a pilot‐scale treatment plant comprised of coagulation–flocculation and filtration processes. Pilot‐scale pretreatment resulted in 99.7% suspended solids removal, 71.5% COD reduction, 68.2% BOD reduction, 100% oil and grease removal and 91% TKN removal. In pilot plant pretreatment, the percentage recovery of water was 83.3%, and 99.7% sludge was recovered after dewatering in a filter press. Copyright © 2006 Society of Chemical Industry     

Chemical Composition of Fruits and Leaves Eaten by Short-Nosed Fruit Bat, Cynopterus sphinx

We evaluated organic and macromineral composition of selected fruits and leaves consumed by the short-nosed fruit bat, Cynopterus sphinx in South India. Results of principal components analysis (PCA) comparing soluble carbohydrates, crude protein, and crude fats indicate a higher percentage of protein in leaves and a higher percentage of carbohydrates and lipids in fruits. However, results of a paired t test comparing these organic components indicated no differences between fruits and leaves. Among the fruits analyzed, Musa x paradisiaca had the highest percentage of carbohydrates, and Psidium guajava had the highest percentage of lipids. Leaves of Cassia fistula, Moringa oleifera, coccinia cordifolia, and F. religiosa had the highest percentage of protein. PCA of selected macrominerals (Ca, Na, K, and P) indicate higher levels of Ca in leaves than in fruits. Results of t tests comparing these macrominerals revealed a difference between fruits and leaves for Ca, but not for the other macrominerals. Among the leaves analyzed, Ca was highest in mature leaves of C. fistula and lowest in leaves of F. religiosa. Leaves of M. oleifera and fruits of Achras sapota were highest in sodium. Among fruits analyzed for macrominerals, Ca was highest in F. bengalensis and lowest in Prosopis juliflora, A. sapota, and M. x paradisiaca. Potassium levels were highest in leaves of C. cordifolia and fruit pods of Prosopis juliflora. Phosphorus content was highest in leaves of M. oleifera and fruits of M. x paradisiaca. The relatively high concentrations of protein and calcium in leaves eaten by C. sphinx supports the hypothesis that leaves are important dietary sources for this plant-visiting bat.     

Removal of dyes by Moringa oleifera seed extract. Study through response surface methodology

BACKGROUND: The effect of pH and initial dye concentration (IDC) on dye removal by coagulation/flocculation process with Moringa oleifera seed extract has been studied. The study was carried out by using the response surface methodology (RSM) in an orthogonal and rotatable design of experiments. Three types of dye were studied: anthraquinonic (Alizarin Violet 3R); indigoid (Indigo Carmine); and azoic (Palatine Fast Black WAN). RESULTS: The interaction level of the two variables studied is higher in the case of azo dye, while it is almost null in the case of anthraquinonic dye. Indigoid dye presents an intermediate situation. pH has a negative influence on dye removal, and by raising IDC q capacity tends to be higher. Polynomic regression of the surface plot was carried out and the adjusted r² found for each case, this being 0.99 in the case of anthraquinonic dye, 0.94 in the case of indigoid dye, and 0.74 in the case of azoic dye. CONCLUSIONS: Moringa oleifera is an interesting natural coagulation agent for use in dye removal. pH should be taken into account in the cases of indigoid and azo dyes, while its influence is rather small in the case of anthraquinonic dye. Copyright © 2009 Society of Chemical Industry     

Coagulation process for tannery industry effluent treatment using Moringa oleifera seeds protein: Kinetic study,pH effect on floc characteristics and design of a thickener unit

The kinetics of coagulation process for turbidity removal from tannery industry effluent using NaCl solution extracted Moringa oleifera protein is reported here. The effect of initial pH of effluent on floc size and strength was analysed. Floc size decrease with increase in velocity gradient and hierarchy of floc size exponent at different pH was: acidic > neutral > basic. It was observed based on the half-life period data that the process follows a second-order kinetic model with 0.000862 (L/mg min) as the rate constant. The Talmadge–Fitch method is used to analyse batch sedimentation data for the calculation of area for the clarifier-thickener unit to treat 5 m³/h of tannery wastewater.     

Comparative Study of Table Margarine Prepared from Moringa oleifera Seed Oil‐Palm Stearin Blend and Commercial Margarines: Composition,Thermal, and Textural Properties

This study aims to produce an oleic acid‐rich table margarine from Moringa oleifera seed oil (MoO)‐palm stearin (PS) blend (70:30, w/w) and compare its composition, thermal behavior, and textural properties during storage with those of commercial margarines (CM1 and CM2). The major fatty acid in MoO/PS blend, CM1 and CM2 is oleic acid (67.85%, 38.54%, and 35.35%, respectively). Hence, many of their triacylglycerols are derived from the acid. MoO/PS blend has a higher complete melting temperature (43.50 °C) compared to CM1 (35.50 °C) and CM2 (35.53 °C). The solid fat content (SFC) of MoO/PS blend at 10 °C (28.7%) is lower than CM1 (32%) and CM2 (68.4%). However, the MoO/PS blend has a higher SFC (6.47%) at 35 °C compared to CMs. At 20 °C, the viscosity of experimental blend margarine (EBM) decreases but CM1 and CM2 increase at the end of the storage study. After 8 weeks of storage, all margarines are harder and CM2 is the hardest. The adhesiveness of EMB and CM2 is similar to the fresh samples while CM1 is more adhesive after storage. In short, it is possible to produce an oleic acid‐enriched margarine from MoO/PS blend that has better textural properties. Practical Applications: Moringa oleifera seed oil is one of the superior oils that contains high levels of oleic acid. However, its high iodine value and low melting point limit its application in the production of margarine. This study shows that direct blending of M. oleifera seed oil with palm stearin could produce margarine with high oleic acid contents and better textural properties in terms of viscosity, hardness, and adhesiveness. The informative data provide supporting evidence for blending of M. oleifera seed oil with palm stearin to produce margarine that could overcome the issues that hinder the M. oleifera seed oil from being produced into margarine.     

THE EFFECT OF ENVIRONMENTALLY BENIGN FRUIT EXTRACT OF SHAHJAN (MORINGA OLEIFERA) ON THE CORROSION OF MILD STEEL IN HYDROCHLORIC ACID SOLUTION

The inhibition of the corrosion of mild steel in hydrochloric acid solution by the fruit extract of shahjan (Moringa oleifera) was studied using weight loss, electrochemical impedance spectroscopy (EIS), linear polarization, and potentiodynamic polarization techniques (Tafel). Inhibition was found to increase with increasing concentration of the extract. The effect of temperature, immersion time, and acid concentration on the corrosion behavior of mild steel in hydrochloric acid solutions with addition of extract was also studied. The inhibition occurred via adsorption of the inhibitor molecules on the mild steel surface obeying the Langmuir adsorption isotherm. Thermodynamic activation parameters such as activation energy, enthalpy (ΔH*), and entropy (ΔS*) of activation for corrosion process were calculated and discussed. The results obtained show that both chemical and physical adsorption of inhibitor molecules occur simultaneously and the fruit extract of shahjan (Moringa oleifera) could serve as an effective inhibitor of the corrosion of mild steel in hydrochloric acid media.     

Synthesis of hydrogels from fractionated proteins of Moringa oleifera Lam. seeds for the treatment of water contaminated with black reactive dye 5

As has been portrayed in the literature, the main proteins extracted from the seeds of Moringa oleifera Lam. have coagulant and clarifying potential in water treatment. However, when using them as coagulant agents, sludge formation occurs, which is an environmental problem. Thus, it was proposed to synthesize hydrogels from the main Moringa seeds proteins and use them as adsorbents. This work aimed to synthesize albumin hydrogels and globulin hydrogels and evaluate their efficiency as adsorbents to remove the reactive black 5 dye. Through FTIR spectrum it was possible to confirm that the synthesis of hydrogels occurred successfully. The kinetic data fit the pseudo-second order model for both hydrogels. The equilibrium isotherms showed that adsorption occurs in multilayers, due to Freundlich fitting. The maximum adsorption capacity occurred at 298 K and was 1.19 mg g⁻¹ for the albumin hydrogel and 10.06 mg g⁻¹ for the globulin hydrogel. Through the results it is possible to state that globulin hydrogel was more favourable for adsorption since it has a more porous morphology and requires less mass compared to albumin hydrogel.     

Extraction and identification of natural antioxidant from the seeds of the Moringa oleifera tree variety of Malawi

The oil from the dried seeds of the Moringa oleifera tree (variety of Malawi) was extracted with a mixture of chloroform/methanol (50∶50). The induction period measurements demonstrated a great resistance to oxidative rancidity. After degumming, there was a reduction of 74% in induction periods. The gums produced were extracted with diethylether, n-butanol, and water, yielding four fractions: Fraction 1 (81.8% w/w), Fraction 2 (0.04% w/w), Fraction 3 (0.05% w/w), and Fraction 4 (17.0% w/w). These fractions were tested for their protection of fresh sunflower oil against rancidity, at 50°C, using a UV accelerated method. The oxidation of the sunflower oil was measured using PV; absorbance E _1cm ^1% and malondialdehyde concentration were measured by HPLC. The fraction that showed the highest antioxidant activity was further fractionated by HPLC, yielding seven fractions. Fraction HPLC 3 (present in a quantity of 330.8 and 29.11 ppm in gums and oil, respectively) showed the highest antioxidant activity. Its activity was also compared with that of the commonly used antioxidants BHT and α-tocopherol on sunflower oil using the same methods. At the same level of addition (200 ppm), HPLC 3 showed higher antioxidant activity than BHT and α-tocopherol. The identification of HPLC3 was done using ¹H NMR, ¹³C NMR, MS, melting point, and UV absorption spectroscopy and proved to be 3,5,7,3′,4′,5′-hexahydroxyflavone (myricetin).     

Evaluation of <Emphasis Type="Italic">Moringa oleifera</Emphasis> seed biopolymer-PVA composite hydrogel in wound healing dressing

Polysaccharide-based hydrogel scaffolds have emerged as promising candidates for skin regeneration. However, for restoring the integrity of the injured tissue, an appropriate balance of moisture and bioactivity needs to be maintained at the wound surface. Though several researchers have designed hydrogels from synthetic or natural resources, their applicability is restricted due to availability, cost and restricted properties suitable to specific wound types. In this study, a novel hydrogel composed of polysaccharide polymer from Moringa oleifera seeds and polyvinyl alcohol (MSP/PVA) was synthesized as a wound dressing material. The hydrogel was characterized using FTIR and XRD and evaluated for its fluid handling properties. It could absorb 5.7 g H₂O g^?1 dry weight with 20–30 % moisture being retained even after 24 h. Its good water absorption and donation ability make it appropriate for dry, necrotic and slightly exuding wounds. Its breathability and water vapor transmission rate extend it applicability to management of burns. The hydrogel exhibited hemocompatibility, antibacterial activity, bacterial impermeability, antioxidant activity and iron chelation that might help healing chronic wounds as well. It was biodegradable. In vivo wound healing showed that the rate of wound reduction and closure was accelerated with rapid re-epithelialization of partial thickness wounds in 6 days without scar formation. In vitro povidone–iodine loading and release studies showed that the iodine release followed Fickian diffusion process. The MSP/PVA hydrogel holds promise for management of dry, necrotic, slightly exuding, chronic wounds, burns and thus appears to be an economical alternative worth industrial exploitation.     

Green lignin-based polyester nanofiltration membranes with ethanol and chlorine resistance

As the main water treatment material, polymeric membranes inevitably suffer from membrane fouling. In this work, novel lignin-based polyester composite nanofiltration membranes (NFM) with ethanol and chlorine resistance were fabricated via interfacial polymerization. Lignin alkali (LA), a green lignin derivative, typically treated as chemical waste in the paper industry, was employed as the aqueous monomer, trimesoyl chloride (TMC) is served as the organic monomer. The structure and separation properties of the lignin-based NFM were studied, revealing that the dense polyester separation layer may show good performance for dye removal. The rejections of the optimized LA/TMC-3 membrane with an excellent permeation flux of 13.9 kg m^?2?h^?1 for rose Bengal sodium salt, brilliant blue, congo red, rhodamine B, MgSO₄, and NaCl are 97.6%, 97.3%, 97.8%, 71.34%, 51.4%, and 31.8%, respectively. Moreover, the LA/TMC-3 membrane also shows long-term tolerance in ethanol and sodium hypochlorite solution; the rejection of LA/TMC-3 to dye only decreases 8% after 8 days when immersed in alcohol, while the normalized rejection maintains 94% after 4000 ppm-hours of continuous exposure to chlorine. This lignin-based polyester membrane may broaden the sustainable utilization sphere of lignin derivatives, at that provide a referable direction for the development of membrane materials.