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为了合成新的、稳定的莱克多巴胺人工抗原,应用Mannich法将莱克多巴胺和载体蛋白BSA经一个亚甲基连接制备莱克多巴胺人工抗原,采用紫外光谱法、蛋白质电泳法、红外光谱法、双抗体夹心法和胶体金免疫层析法鉴定,证明莱克多巴胺人工合成抗原偶联成功、与抗莱克多巴胺抗体有特异性反应、具有抗原性。Mannich法合成的莱克多巴胺人工抗原未见报道,莱克多巴胺人工抗原用作包被抗原制成的莱克多巴胺快速检测试纸条对莱克多巴胺的检测灵敏度约为5 ng/mL,也可作为免疫源性抗原用于制备抗莱克多巴胺抗体。 相似文献
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应用多壁碳纳米管(MWCNTs)、L-精氨酸(L-Arg)和纳米金(Au NPs)修饰玻碳电极(GCE)获得Au NPs/P-L-Arg/MWCNTs/GCE修饰电极,通过静电作用将氨基修饰的莱克多巴胺(RAC)适配体固定于电极表面,制得新型莱克多巴胺适配体电化学传感器。考查了扫描速度、培育温度、培育时间、适配体修饰量以及p H等条件对传感器响应性能的影响。结果表明,该传感器灵敏度高、选择性好。在优化条件下,其线性范围为1.00×10-11~1.00×10-7mol/L,线性相关系数为0.980 4,检出限达1.00×10-11mol/L。另外,该传感器操作简单、性能稳定,将其用于实际猪尿中莱克多巴胺的检测,回收率为97.1%~103.0%,相对标准偏差低于9.4%。 相似文献
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《山东化工》2015,(16)
在石墨烯修饰的玻碳电极表面滴涂莱克多巴胺分子印迹聚合物,制备了莱克多巴胺分子印迹电化学传感器。优化了石墨烯浓度、分子印迹聚合物浓度、富集时间、p H值等对传感器的影响,优化后得到的最佳实验条件为:石墨烯浓度为0.3 mg/m L,莱克多巴胺分子印迹聚合物浓度为3.0 mg/m L,富集时间为5 min,磷酸盐缓冲溶液的p H值为7.0;在最佳条件下,石墨烯/分子印迹聚合物的电化学传感器对莱克多巴胺进行检测,其峰电流响应与莱克多巴胺的浓度在0.5~90.0μmol/L范围内具有良好的线性关系,检测限为0.16μmol/L(S/N=3)。石墨烯/分子印迹聚合物的电化学传感器成功应用于猪肉样品中莱克多巴胺含量的测定。 相似文献
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制备抗克仑特罗、沙丁胺醇和莱克多巴胺双特异性抗体并初步鉴定。将分泌抗克仑特罗、沙丁胺醇抗体的细胞株和分泌抗莱克多巴胺抗体的细胞株分别驯化,使之成为HAT敏感株。将两者融合,筛选分泌双特异性单克隆抗体的杂交-杂交瘤株,然后对其进行初步鉴定。共获得5株杂交-杂交瘤细胞株,将其分泌的双特异性单克隆抗体(BsAb)经过酶联免疫吸附法(ELISA)初步检测,IC50可达1 ng/mL,腹水效价为105以上,用ELISA法证明BsAb只与克仑特罗、沙丁胺醇和莱克多巴胺有特异性反应。该方法制备简单、风险低、灵敏度高、周期短,具有较好的应用前景。 相似文献
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乙烯酮(双乙烯酮)是十分重要的化工中间体,其下游产品较多。江苏某化工厂开发生产乙烯酮(双乙烯酮)下游产品三十多个,年生产规模三万多吨,是国内以乙烯酮(双乙烯酮)为中间体生产精细化学品的综合骨干企业。针对乙烯酮(双乙烯酮)下游产品废水特点,该厂结合企业实际,开展了产品优化,结构调整,清洁生产,资源循环利用,节水降耗等工作,从源头削减了污染物的生产。同时投资二千多万元新建预处理装置三套,6000m3/d废水生化处理装置一套,使全厂乙烯酮(双乙烯酮)下游产品的废水得到了有效的治理。 相似文献
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The objective of the study was to explore the effect of the degree of deacetylation (DD) of the chitosan used on the degradation rate and rate constant during ultrasonic degradation. Chitin was extracted from red shrimp process waste. Four different DD chitosans were prepared from chitin by alkali deacetylation. Those chitosans were degraded by ultrasonic radiation to different molecular weights. Changes of the molecular weight were determined by light scattering, and data of molecular weight changes were used to calculate the degradation rate and rate constant. The results were as follows: The molecular weight of chitosans decreased with an increasing ultrasonication time. The curves of the molecular weight versus the ultrasonication time were broken at 1‐h treatment. The degradation rate and rate constant of sonolysis decreased with an increasing ultrasonication time. This may be because the chances of being attacked by the cavitation energy increased with an increasing molecular weight species and may be because smaller molecular weight species have shorter relaxation times and, thus, can alleviate the sonication stress easier. However, the degradation rate and rate constant of sonolysis increased with an increasing DD of the chitosan used. This may be because the flexibilitier molecules of higher DD chitosans are more susceptible to the shear force of elongation flow generated by the cavitation field or due to the bond energy difference of acetamido and β‐1,4‐glucoside linkage or hydrogen bonds. Breakage of the β‐1,4‐glucoside linkage will result in lower molecular weight and an increasing reaction rate and rate constant. © 2003 Wiley Periodicals, Inc. J Appl Polym Sci 90: 3526–3531, 2003 相似文献
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D. G. Gordeev L. F. Gudarenko M. V. Zhernokletov V. G. Kudel’kin M. A. Mochalov 《Combustion, Explosion, and Shock Waves》2008,44(2):177-189
A semi-empirical equation of state for metals is described. Its capabilities are demonstrated by the example of the equation
of state for aluminum. New experimental data are presented on the location of the isentrope of aluminum for unloading from
the state at p = 229.71 GPa on the shock adiabat to an aerogel (SiO2) of density 0.08 g/cm3.
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Translated from Fizika Goreniya i Vzryva, Vol. 44, No. 2, pp. 61–75, March–April, 2008. 相似文献
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Jorge Marcelo Romero Soledad Bustillo Hugo Enrique Ramirez Maisuls Nelly Lidia Jorge Manuel Eduardo Gómez Vara Eduardo Alberto Castro Alicia H. Jubert 《International journal of molecular sciences》2007,8(7):688-694
A thermochemical rather simple experimental technique is applied to determine the enthalpy of formation of Diperoxide of ciclohexanone. The study is complemented with suitable theoretical calculations at the semiempirical and ab initio levels. A particular satisfactory agreement between both ways is found for the ab initio calculation at the 6–311G basis This set level. Some possible extensions of the present procedure are pointed out. 相似文献
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杀虫磺缓释剂杀钉螺试验报告 总被引:1,自引:0,他引:1
杀虫磺缓释剂浸杀成螺、幼螺、螺卵效果好,现场应用缓释片63克/米^2,半年后活螺平均密度下降93.2%,春季施药1次,可达到控制当年钉螺的扩散。 相似文献
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醋酸纤维素取代基分布与性质的关系 总被引:9,自引:0,他引:9
分析了以吡啶为溶剂的醋酸纤维素的13C-NMR核磁共振谱,得出了三种不同位置羟基的取代度。结合X—射线和DSC分析,初步说明具有相同取代度但未经水解和经过水解的醋酸纤维素(CA) 性质上的差异是由于三个羟基上的取代度分布不同及消晶程度差异所致。 相似文献