大型玻璃钢渔船的开发及应当注意的几个问题

<正> 1 概述由于玻璃钢渔船具有许多优点,目前世界上玻璃钢渔船发展非常迅速。日本在这方面处于领先地位,日本注册的玻璃钢渔船数目已超过33万多艘,占渔船总数的85%以上,其中包括某些长达45m的船体。其它发达国家的玻璃钢渔船发展也非常迅速。我国于1958年开始小型玻璃钢船的制造,但玻璃钢渔船起步较晚,1974年才建造第一对玻璃钢渔船。1983年开始,我所与中国水产科学院渔船室进行了玻璃钢渔船的专题研究,并设计丁几种主要船型。通过十几年的使用,玻璃钢渔船性能良好,取得了明显的效果。但由于各     

我国将示范推广玻璃钢渔船标准船型

<正>全国玻璃钢渔船产业技术发展联盟今年重点推进辽宁省玻璃钢渔船标准化船型示范推广中心筹建工作,并在辽宁全省试点示范推广玻璃钢渔船标准船型。据全国玻璃钢渔船产业技术发展联盟理事长、中国水产科学研究院渔业机械仪器研究所所长徐运皓介绍,2015年,该联盟下设的玻璃钢渔船标准化船型推广中心将主要做好     

国内外玻璃钢渔船的发展

本文介绍国内外玻璃钢渔船的发展概况．并提出了玻璃钢渔船应注意的问题。     

33m玻璃钢拖网渔船纵向挠度测试报告

<正> 1 试验目的玻璃钢渔船在我国设计、生产、使用已具有一定的规模,尤其是在近几年发展较快,在山东威海等地已生产了数十条20～30m的玻璃钢渔船。玻璃钢渔船与同类型的钢质渔船相比具有重量轻、速度快、维修方便、维修费用低、捕捞量大、经济效益显著等优点,从而深受广大渔民的欢迎。我所按照《玻璃纤维增强塑料渔船建造规范》设计了多种规格的玻璃钢渔船,但已建造的     

借鉴台湾经验 提速渔船玻璃钢化改造

渔船玻璃钢化改造是发展绿色捕捞业的重要内容和普遍趋势,纵观周边海洋捕捞业发达的国家和地区,中小型渔船玻璃钢化率都超过50%,但大陆渔船玻璃钢化改造却面临渔民经济基础薄弱、对玻璃钢渔船认识不足等多重问题,以致拥有大量产能,改造进程却始终缓慢。其实,类似的情况在台湾岛内也曾出现,并在相关部门政策鼓励下得到了良好解决。     

玻璃钢渔船的开发与应用

<正> 一、概况世界上最早使用玻璃钢渔船的国家是美国.1959年美国海运工业船厂率先研制成功了9米长玻璃钢捕虾船,以后玻璃钢渔船的研究开发工作一直进行得很活跃.1966年开始,25米左右的玻璃钢拖网渔船已能成批生产.现在18米以下的木质渔船已全部淘汰. 到目前为止,玻璃钢渔船发展最快的国家是日本.日本从1960年到1992年32年     

玻璃钢渔船结构设计研究报告

<正> 1 概述用玻璃钢建造渔船是本世纪50年代末,60年代初发展起来的一项新技术。玻璃钢渔船的出现,改变了人们千百年来采用木材等常规材料建造渔船的历史,因此也是渔船发展史上的一次重大变革。渔船采用玻璃钢建造后,除了能节省大量的优质木材,有利于保护森林资源和生态环境外,同时还能取得比木质渔船更好的经济效益,所以一直受到世界各国渔业部门的普遍关注,并有逐步取代木质渔船的趋势。     

我国玻璃钢渔船的现状问题与前景

我国玻璃钢渔船的现状问题与前景张崇杰（上海玻璃钢研究所２００１２６）１现状我国玻璃钢渔船的开发研究工作开展得比较晚。从１９７４年天津塘沽捕捞船厂建造了我国第一艘本质骨架、玻璃钢船壳的海洋捕捞渔船后，才引起有关方面的注意。初期建造的玻璃钢渔船，大多采用...     

日本玻璃钢渔船的现状

日本玻璃钢渔船的历史已有15年了。从77年末的统计资料看,海水动力渔船约为117000条,淡水动力渔船约为16000条。它们占总渔船数的三分之一左右。日本是世界上玻璃钢渔船最多的国家。将来日本要使363000条渔船都是玻璃钢渔船。     

33m玻璃钢拖网渔船总纵刚度、强度测试报告

<正> 1 试验目的玻璃钢渔船在我国设计、生产、使用已具有一定的规模,尤其是在近几年发展较快,在山东威海等地已生产了数十条20～30m的玻璃钢渔船。玻璃钢渔船与同类型的其它材质渔船相比较具有重量轻、速度快、维修方便、维修费用低、捕捞量大、经济效益显著等优点,从而深受广大渔民的欢迎。我所按照《玻璃纤维增强塑料渔船建造规范》设计了多种规格的玻璃钢渔船,但已建造的玻璃钢渔船的各项技术指标与设计值之间的差别,我们只有通过测试才能得知。我们在山东西港渔业公司威海小石岛船厂的大力协助下,于1999年5月对该厂生产的33m玻璃钢拖网渔船进行了在自重作用下的总纵刚度、强度的静态测试,测得了该船在自重作用下所选各点的挠     

热管型船用吸附制冰机的设计

A heat pipe type adsorption ice maker with two adsorbers for fishing boats is designed by using ammonia as refrigerant and compound of activated carbon-CaCl2 as adsorbent. This type of heat pipe adsorber can solve the problem of incompatibility between ammonia, copper, seawater and steel. The working process of the ice maker with 8.7kg adsorbent per bed is simulated. The results show that the optimal semi-cycle time is about 9 min at the evaporating temperature of -15℃, where the corresponding cooling power, specific cooling power per kilogram adsorbent SCP and coefficient of refrigerant performance COP are respectively 3.6kW, 217W·kg-1 and 0.404.     

输电用玻璃纤维复合材料杆塔技术开发与应用

介绍了玻纤复合材料杆塔的优势和发展关键技术,探讨相应杆塔结构设计及材料技术、电气结构及线路设计技术、生产制备技术和工程应用技术发展情况,调研了国内外复合材料输电杆塔应用状况,展望目前复合材料杆塔可能存在的问题及未来发展的方向。     

Clinicopathologic and Prognostic Association of GRP94 Expression in Colorectal Cancer with Synchronous and Metachronous Metastases

Patients with advanced colorectal cancer (CRC) with distant metastases have a poor prognosis. We evaluated the clinicopathological relevance of GRP94 expression in these cases. The immunohistochemical expression of GRP94 was studied in 189 CRC patients with synchronous (SM; n = 123) and metachronous metastases (MM; n = 66), using tissue microarray; the association between GRP94 expression, outcome, and tumor-infiltrating lymphocytes (TILs) was also evaluated. GRP94 was expressed in 64.6% (122/189) patients with CRC; GRP94 positivity was found in 67.5% and 59.1% patients with SM and MM, respectively. In the SM group, high GRP94 expression was more common in patients with a higher density of CD4+ TILs (p = 0.002), unlike in the MM group. Survival analysis showed that patients with GRP94 positivity had significantly favorable survival (p = 0.030); after multivariate analysis, GRP94 only served as an independent prognostic factor (p = 0.034; hazard ratio, 0.581; 95% confidence interval, 0.351–0.961) in the SM group. GRP94 expression was detected in 49.4% of metastatic sites and showed significant heterogeneity between primary and metastatic lesions (p = 0.012). GRP94 is widely expressed in CRC with distant metastases; its expression was associated with favorable prognosis in the SM group, unlike in the MM group.     

玻璃钢法兰的结构设计及成型工艺

根据玻璃纤维增强塑料的性能特点，结合实际经验，探讨了玻璃纤维增强塑料法兰的结构及成型工艺。结果表明，此种法兰的结构设计及其成型方法可行，已获得应用。     

Interaction of titanium diboride/boron nitride evaporation boats with aluminium

Hexagonal boron nitride/titanium diboride composites are widely used as evaporation boats for aluminium deposition to produce functional and decorative layers on different target materials. The lifetime of such a material is limited mainly by the interaction of the metal with the ceramic substrate, but the corrosion mechanism has still not yet been thoroughly investigated and understood. In this article the corrosion mechanism for the evaporation boats used was investigated using thermodynamic calculations, FESEM, EDX and XRD phase analysis. The analysis showed that hexagonal boron nitride (hBN), which is thermodynamically less stable than TiB₂, is passivated during the application process through the formation of AlN surface layers, whereas the thermodynamically more stable TiB₂ phase dissolves and Ti-rich components precipitate in cooler regions of the evaporation boats.     

Upregulation of Phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in Acquired Middle Ear Cholesteatoma Growth

Cholesteatoma is a destructive and expanding growth of keratinizing squamous epithelium in the middle ear or petrous apex. The molecular and cellular processes of the pathogenesis of acquired middle ear cholesteatoma have not been fully understood. In this study, comparative proteomic analysis was conducted to investigate the roles of specific proteins in the pathways regarding keratinocyte proliferation in cholesteatoma. The differential proteins were detected by comparing the two-dimension electrophoresis (2-DE) maps of the epithelial tissues of 12 attic cholesteatomas with those of retroauricular skins. There were 14 upregulated proteins in the epithelial tissues of cholesteatoma in comparison with retroauricular skin. The modulation of five crucial proteins, HSP27, PRDX2, GRP75, GRP78 and GRP94, was further determined by RT-PCR, Western blot and immunohistochemistry. Phosphorylation of HSP27 at Ser-82 was identified by mass spectroscopy. The results of this study suggested that phosphorylated HSP27 is the end expression of two potential signal-transduction pathways, and together with PRDX2, they are very likely involved in the proliferation of keratinocytes in cholesteatoma. Upregulations of GRP75, GRP78 and GRP94 in keratinocytes may be able to counter endoplasmic reticulum stress, to inhibit cell apoptosis, to prevent protein unfolding and to promote cholesteatoma growth.     

VARTM工艺玄武岩纤维船舶成型技术研究

本文主要研究了通过VARTM工艺利用新型玄武岩纤维制造船舶的重要技术。介绍了船体设计及其铺层设计。通过对玄武岩渗透率试验、导流网导流试验以及泡沫夹层试验试验结果的分析,找出影响玄武岩纤维制造船舶的关键因素以及解决成型困难的途径。也为解决制作玄武岩纤维大型制品或构件成型工艺面临的难题提供解决途径,具有较大的实际价值与意义。     

A low-cost affinity purification system using β-1,3-glucan recognition protein and curdlan beads

Silkworm β-1,3-glucan recognition protein (βGRP) tightly and specifically associates with β-1,3-glucan. We report here an affinity purification system named the 'GRP system', which uses the association between the β-1,3-glucan recognition domain of βGRP (GRP-tag), as an affinity tag, and curdlan beads. Curdlan is a water-insoluble β-1,3-glucan reagent, the low cost of which (about 100 JPY/g) allows the economical preparation of beads. Curdlan beads can be readily prepared by solubilization in an alkaline solution, followed by neutralization, sonication and centrifugation. We applied the GRP system to preparation of several proteins and revealed that the expression levels of the GRP-tagged proteins in soluble fractions were two or three times higher than those of the glutathione S-transferase (GST)-tagged proteins. The purity of the GRP-tagged proteins on the curdlan beads was comparable to that of the GST-tagged proteins on glutathione beads. The chemical stability of the GRP system was more robust than conventional affinity systems under various conditions, including low pH (4-6). Biochemical and structural analyses revealed that proteins produced using the GRP system were structurally and functionally active. Thus, the GRP system is suitable for both the large- and small-scale preparation of recombinant proteins for functional and structural analyses.     

Enhanced Antitumor Effects of Adenoviral-Mediated siRNA against GRP78 Gene on Adenosine-Induced Apoptosis in Human Hepatoma HepG2 Cells

Our previous studies show that adenosine-induced apoptosis is involved in endoplasmic reticulum stress in HepG2 cells. In this study, we have investigated whether knockdown of GRP78 by short hairpin RNA (shRNA) increases the cytotoxic effects of adenosine in HepG2 cells. The adenovirus vector-delivered shRNA targeting GRP78 (Ad-shGRP78) was constructed and transfected into HepG2 cells. RT-PCR assay was used to determine RNA interference efficiency. Effects of knockdown of GRP78 on adenosine-induced cell viabilities, cell-cycle distribution and apoptosis, as well as relative protein expressions were determined by flow cytometry and/or Western blot analysis. The intracellular Ca²⁺ concentration was detected by laser scanning confocal microscope. Mitochondrial membrane potential (ΔΨm) was measured by a fluorospectrophotometer. The results revealed that GRP78 mRNA was significantly downregulated by Ad-shGRP78 transfection. Knockdown of GRP78 enhanced HepG2 cell sensitivity to adenosine by modulating G0/G1 arrest and stimulating Bax, Bak, m-calpain, caspase-4 and CHOP protein levels. Knockdown of GRP78 worsened cytosolic Ca²⁺ overload and ΔΨm loss. Knockdown of caspase-4 by shRNA decreased caspase-3 mRNA expression and cell apoptosis. These findings indicate that GRP 78 plays a protective role in ER stress-induced apoptosis and show that the combination of chemotherapy drug and RNA interference adenoviruses provides a new treatment strategy against malignant tumors.