卟啉及其卟啉基聚合物光动力学的比较研究

光动力疗法（Photodynamic Therapy，PDT）是不同于手术、放疗、化疗和免疫治疗的一种崭新疗法，对血管增生性病变组织的选择性治疗具有很好的疗效。本文比较了三种卟啉及卟啉基聚合物的生物相容性和光动力学疗效。MTT测试结果显示，在非光照下Por、Por-PNIPAM、Por-PNIPAM-b-POEGMA对小鼠成纤维细胞L929和人宫颈癌细胞HeLa的毒性均较低；而在红光照射下，Por、Por-PNIPAM、Por-PNIPAM-b-POEGMA对L929细胞毒性较低，但对HeLa细胞毒性较高。     

Biocompatibility of MIM 316L stainless steel

To evaluate the biocompatibility of MIM 316L stainless steel, the percentage of S-period cells were detected by flow cytometry after L929 incubated with extraction of MIM 316L stainless steel, using titanium implant materials of clinical application as the contrast. Both materials were implanted in animal and the histopathological evaluations were carried out. The statistical analyses show that there are no significant differences between two groups (P>0.05), which demonstrates that MIM 316L stainless steel has a good biocompatibility. Foundation item: Project (2003AA302210) supported by the National Hi-tech Research Program of China     

Biocompatibility of FGL peptide self-assembly nanofibers with neural stem cells in vitro

In order to study the biocompatibility of self-assembled FGL peptide nanofibers scaffold with neural stem cells (NSCs), FGL pepitide-amphiphile (FGL-PA) was synthesized by solid-phase peptide synthesis technique. The diluted hydrochloric acid was added into FGL-PA solution to reduce the PH value and accordingly induce self-assembly. The morphological features of the assembled material were studied by transmission electron microscope. NSCs were cultured and added with self-assembled FGL-PA. CCK-8 kit was used to test its effect on the proliferation of NSCs. The differentiation of NSCs was also tested after FGL-PA assembled material added. The experimental results showed that FGL-PA could be self-assembled to form a hydrogel. TEM analysis showed the self-assembled hydrogel was nanofibers with diameter of 10–20 nm and length of hundreds nanometers. FGL-PA with concentrations of 50,100, or 200 mg/L could promote the proliferation of NSCs, and absorbance of them was increased (P<0.05). The rate of neurons differentiated from NSCs was improved greatly by FGL-PA assembled material compared with control (P<0.05). The findings suggested that FGL-PA could self-assemble to nanofiber hydrogel, which had good biocompatibility with NSCs.     

温度响应性玻璃球基复合载体的制备及表征

报道了一种新型的智能响应性玻璃球基复合载体的制备方法。首先通过自由基聚合法将双键类单体N⁃异丙基丙烯酰胺、甲基丙烯酸羟丙酯和甲基丙烯酸（3⁃三甲氧基硅）丙酯合成随机嵌段共聚物；然后借助自制的底喷式流化床反应器将共聚物溶液均匀喷涂于玻璃球基表面，并利用高温退火作用实现共聚物与玻璃球基之间的键合，从而形成共聚物/玻璃球基复合载体。同时，对共聚物在玻璃球基表面的包衣效果进行初步考察，包括特征官能团、表面微观结构、生物相容性和温度响应性等。结果表明，通过底喷包衣技术可将温度响应性共聚物链接于玻璃球基表面，且能形成一定厚度的膜层。这种复合载体具有良好的生物相容性，能利用其智能响应特性实现细胞的有效黏附和自发脱附。     

含羧基和磺酸基共聚物阻垢剂的合成及阻垢性能

为了抑制在工业设备中的硫酸钙沉淀,各类新型阻垢剂被投入生产使用. 以马来酸酐（MA）、2-丙烯酰胺基-2-甲基丙磺酸（AMPS）为原料,过二硫酸钾（K2S2O8）为引发剂合成二元共聚物马来酸酐-2-丙烯酰胺-2-甲基丙磺酸（PMA-PAMPS）. 采用FT-IR、元素分析等技术对其结构进行了表征. 并分析测定了PMA-PAMPS在不同浓度、温度条件下的阻垢性能. 结果表明,在恒温60 ℃下,钙离子含量为1.510 g/L的液体中加入3 mg/L的阻垢剂,其阻垢率达到92.09%,且在60～80 ℃环境下也具备良好的阻垢性能. 硫酸钙晶体的电镜扫描结果表明PMA-PAMPS对硫酸钙有良好的阻垢效果.     

AM/AA/AMPS/AMC_(14)S共聚物的制备与表征

以丙烯酰胺(AM)单体为主要原料,引入疏水缔合单体2-丙烯酰胺基十四烷磺酸(AMC14S)和辅助共聚单体2-丙烯酰胺基-2-甲基丙磺酸(AMPS),选择氧化还原/水溶性偶氮化合物复合引发体系,采用胶束聚合技术和前加碱共水解法,制备了耐温抗盐驱油聚合物AM/AA(丙烯酸)/AMPS/AMC14S产品。考察了偶氮化合物W-58、单体AMC14S用量对共聚物相对分子质量的影响,并表征了共聚物的结构特征。实验结果表明,当偶氮化合物W-58用量为3.0×10-4g.g单体-1、AMC14S质量分数为0.2%~0.5%时,共聚物的相对分子质量最高,耐温抗盐性能较好。     

Synthesis of size-controllable Fe_3O_4 magnetic submicroparticles and its biocompatible evaluation in vitro

Large scaled uniform and size-controllable magnetic submicroparticles(MSPs) were synthesized via solvothermal method with ferric chloride as iron source and sodium acetate as trapping agent. The influence of Fe~(3+) and Na Ac contents on the size distribution of MSPs was investigated. The structural and morphological properties of the synthesized particles were studied by scanning electron microscopy(SEM), X-ray power diffraction(XRD) and vibrating sample magnetometer(VSM). The well-dispersed MSPs with size of 100-1000 nm were obtained by simply adjusting the contents of Fe~(3+) and NaA c. In addition, the hemolysis and cytotoxicity of Fe_3O_4 MSPs, and their ability to case arrest in cell life-cycles were studied. The results indicate that larger size could lead to lower hemolysis. From MTT(3-(4,5-dimethylthuazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the interactions between MSPs and adhesive mouse fibroblast cell line(L929) were probed. Larger size of Fe_3O_4 MSPs demonstrates lower cell viability following an exposure to the cells.     

生物功能化AgInS_2量子点的制备及其生物应用

为制备水相、低毒、生物兼容性较好的三元量子点,通过水相合成法制备三元银铟硫(AgInS_2)量子点,并用叶酸(FA)对其进行修饰,利用透射电子显微镜、紫外-可见-近红外分光光度计、荧光分光光度计、傅里叶红外光谱仪等表征手段对纳米复合材料的形貌、光学性质、化学组成、细胞毒性及荧光成像进行了研究。结果表明,水相AgInS_2量子点的尺寸约为5nm~6nm,经叶酸(FA)修饰后,细胞毒性较低,在乳腺癌细胞中成像时红色荧光更明显,说明通过叶酸的修饰可提高量子点的生物兼容性。因此这类具有较好水溶性和生物兼容性的FA-AgInS_2量子点可直接应用于生物成像研究,为乳腺癌的快速体外检测打下了良好的实验基础。     

Mg-Zn-Y-Nd coated with citric acid and dopamine by layer-by-layer self-assembly to improve surface biocompatibility

Magnesium alloy has been generally accepted as an important biodegradable material on cardiovascular stent development for a long time. However, its limited biocompatibility, especially delayed endothelialization process restricts its further application. In this contribution, we modified the Mg-Zn-Y-Nd alloy surface with citric acid and dopamine via a layer-by-layer self-assembly assay, aiming at improving the biocompatibility of the magnesium alloy. The citric acid/dopamine (CA/PDA) layer exhibited a remarkable suppression of platelet activation/aggregation and thrombosis under 15 dyn/cm² blood flowing. Inhibition on vascular smooth muscle cells growth and macrophages attachment/activation were also observed on this layer. In particular, the CA/PDA layer presented a promoted property for the vascular endothelial cells growth and spreading compared with the bare magnesium alloy, suggesting the pro-endotelialized function. In conclusion, this research may support potential application on surface modification of magnesium alloy based cardiovascular stents for better biocompatibility.     

Biocompatibility of Compounds of Extracellular Matrix and Thermally Reversible Hydrogel

A new scaffold material composed of extracellular matrix (ECM) and thermal sensitive hydrogel (HG), and evaluated its biocompatibility were investigated. We cultured bladder smooth muscle cells with this compound material, and then observed with phase contrast microscopy and scanning electron microscope (SEM) to assess the cell growth and morphology. The cell adhesion and proliferation were detected with MTT assay and cell count. Results show the ECM/HG compounds appeared as a net-like and red-stained construction with enough meshes and without any cellular fragments. 6 h after implantation, cells were observed adhere on the compounds and extend spurious along the fibers 12 h later. Under SEM even some ECM was observed to be secreted. MTT assay shows there was obvious statistic difference among 3 groups (P＜0.05).ECM/HG compound materials show a good biocompatibility, which confirms that it would be an ideal tissue engineering scaffolds.     

药用卤化丁基胶塞溶血试验

目的：研究药用丁基胶塞的溶血性能。方法：采用紫外分光光度法测定溶血率。结果溶血率均低于5%。结论：药用丁基胶塞在急性溶血方面,具有良好的生物安全性。     

In vitro biocompatibility assessment of a novel PRGD/PDLLA/NGF composite material

The objective of this study was to evaluate the degradability and biocompatibility of a novel composite materials which was grafted with RGD and immobilized with NGF(PRGD/PDLLA/NGF). The releasing of NGF, the biodegradability and cell-biocompatibility of PRGD/PDLLA/NGF membrane were evaluated in vitro. The experimental results showed that the NGF release process was prolonged over 30 days. Furthermore, the PRGD/PDLLA/NGF showed a better hydrophilicity, better biodegradation properties and cells affinity than PDLLA, which means a good support to adhesion and proliferate of Schwann cells. Therefore, the novel composite material holds considerable promise as scaffolds in nerve tissue engineering.     

The Study of Nerve Conduit with Biocompatibility and Electrical Stimulation Effect

Conductive polymers show great promise because of their electrical property based on bioelectricity in vivo. In order to search electro-activity materials which insure abduction of tissue, we synthesized conductive nerve conduits with poly-dl-lactic-acid(PDLLA) and tetra-aniline(TA). Preparation technology of TA was optimized, and the properties of the conduit were studied. RSC96 cell were used to investigate the toxicity and electrical stimulation effect. SD rats were used to assess biocompatibility in vivo. The results showed that the reaction ratio of 1:1, the reaction time of 2 h and the HCl concentration of 2 mol/L were the optimum conditions for synthesis of TA. The influence of TA content on the mechanical properties, hydrophilicity, conductivity and microstructure of the nerve conduit was evaluated. Cell and histocompatibility study showed PDLLA/TA possessed good biocompatibility. These results showed it had application values in tissue projects.     

PAN-g-MAPEG接枝共聚物的制备及性能

采用大单体技术,将马来酸聚乙二醇单酯大单体与丙烯腈进行接枝共聚,合成了以聚丙烯腈为主链、马来酸聚乙二醇单酯为支链的新型接枝共聚物。通过采用DSC、步冷曲线和特性黏度测试表征了接枝共聚物的热性能、保温性能和相对分子质量。结果表明,PAN-g-MAPEG的结晶焓和熔融焓为49.92和53.43J/g,分别为理论值的70.95%和64.24%;在反复的相变过程中,吸热、放热稳定,热稳定性好;接枝共聚物的保温性能良好,保温平台大约出现在35℃,持续大约6min;接枝共聚物的相对分子质量达到71 431。     

Cytotoxicity, Degradation and Release Behavior of Alginate Fibers Loaded with Chlorhexidine Gluconate and Its Docking Simulation

To investigate the feasibility and safety of alginate fibers loaded with chlorhexidine gluconate (CHG) for biomedical applications, vitro cytotoxic test and drug release pattern were studied. Discovery Studio (DS), a computer aided software, was utilized to briefly investigate the energy state and solvation at the molecular level. Alginate fiber loaded with CHG presented different drug release patterns in PBS and A solution. Cytotoxicity results indicated that CHG-loaded alginate fibers can promote the growth of L-929 mouse fibroblast cells with better biocompatibility. Through the simulation, the lowest energy conformation of three representative molecular bond regions was optimized with stronger stability. As the torsional energy increased, the bond energy of the target regions decreased. Besides, the transition energy of the target regions was greatly reduced by solvation. In a word, alginate fibers loaded with CHG expressed better biocompatibility and drug sustained-release ability, which indicates that CHG-loaded alginate fibers has a broad application prospect in medical dressing.     

景天三七总酚酸的提取工艺及抗氧化性研究

超声波辅助提取景天三七中的总酚酸,用分光光度法测定其提取率。探讨提取时间、提取温度、料液比、乙醇浓度对景天三七总酚酸提取工艺的影响,在单因素提取的基础上采用正交的方法优化提取条件。结果表明,影响景天三七总酚酸提取工艺的主要因素为提取温度,其次为提取时间、料液比、乙醇浓度。最佳提取工艺条件为提取温度60℃、提取时间60min、料液比1:8、乙醇浓度70%,测得的景天三七总酚酸提取率为3.62%.景天三七总酚酸的抗氧化能力与Vc相接近,表明景天三七具有良好的抗氧化能力。     

Cell growth and desorption on the surface of temperaturesensitive semi-IPNs hydrogels based on silk sericin

Semi-interpenetrating (semi-IPNs) hydrogels containing biocompatible silk sericin (SS) and poly(N-isopropylacrylamide)(PNIPAM) were prepared as novel cellular matrices. Their maximum swelling degree and basic characteristics for biomedical applications such as mouse ?broblasts (L929) cell proliferation and desorption were investigated. The results showed that the incorporation of high hydrophilic SS into PNIPAM hydrogel increased the maximum swelling degree of the semi-IPNs hydrogels, and the adhesion and growth of the L929 on semi-IPNs hydrogels were at least comparable to, or even better than, that on conventional PNIPAM hydrogel. In addition, L929 cells were found to detach from the hydrogels surface naturally by controlling environmental temperature. These results suggest great potential of semi-IPNs hydrogels in tissue engineering.     

Waterborne Epoxy Resin Modified by AMPS

A stable epoxy emulsion was prepared with epoxy resin （EP） as raw material, 2-acrylamido-2- methyl-l-propanesulfonic acid （AMPS） as modifier and benzoyl peroxide as initiator. By criterion of yield of the copolymer AMPS-EP, water-solubility, change of the acid value and intrinsic viscosity [η] along with reaction time, the copolymerization course was deduced. It is found that during the process, AMPS takes part in both the grafting copolymerization with epoxy principal chain and the ring-opening polyaddition with epoxy group. It is also discovered that the yield of AMPS-EP and water dispersing varies with reaction time. When it reaches 1.5 h, AMPS-EP can obtain good water-solubility; but the water-solubility will go bad gradually if it exceeds 3.5 h.. R spectrum analysis indicates that partial epoxy group partially remains and the others create sulfonic ester.     

Strength of copolymer grouting material based on orthogonal experiment

Using the orthogonal experimental design method involving three factors and three levels, the flexural strength and the compressive strength of copolymer grouting material were studied with different compositions of water-cement ratio (mass fraction of water to cement), epoxy resin content, and waterborne epoxy curing agent content. By orthogonal range and variance analysis, the orders of three factors to influence the strength, the significance levels of different factors, and the optimized compound ratio scheme of copolymer grouting material mixture at different curing ages were determined. An empirical relationship among the strength of copolymer grouting material, the water-cement ratio, the epoxy resin content, and the waterborne epoxy curing agent content was established by multivariate regression analysis. The results indicate that water-cement ratio is the most principal and significant influencing factor on the strength. Epoxy resin content and waterborne epoxy curing agent content also have a significant influence on the strength. But epoxy resin content has a greater influence on the 7-day and 28-day flexural strength, and waterborne epoxy curing agent content has a greater influence on the 3-day flexural strength and the compressive strength. The copolymer grouting material with water-cement ratio of 0.4, epoxy resin content of 8% (mass fraction) and waterborne epoxy curing agent content of 2% (mass fraction) is the best one for repairing of cement concrete pavement. The flexural strength and the compressive strength have good correlation, and the ratio of compressive strength to flexural strength is between 1.0 and 3.3. Foundation item: Projects(40728003, 40772180, 40802064) supported by the National Natural Science Foundation of China; Project (07JJ4012) supported by the Hunan Provincial Natural Science Foundation of China; Project(20080430680) supported by China Postdoctoral Science Foundation; Project(B308) supported by Shanghai Leading Academic Discipline Project     

一种胶原支架材料的结构与性能表征

针对脱细胞真皮基质(acellular dermal matrix, ADM)孔径小、孔隙率低、孔隙的连通性不理想的难题,以猪中厚皮为原料,综合碱处理、酶处理、去污剂处理和盐处理手段制备了一种支架材料,通过H＆E染色、红外与紫外可见光谱、扫描电子显微镜、拉力试验、MTT法等手段分析了支架材料的性能。结果显示:支架材料维持了胶原的天然结构,不含细胞成分,紫外与红外吸收符合胶原蛋白的吸收特征,胶原质量分数为(83.19±0.24)％,为具有连通孔道的三维网络结构,孔径约100μm,孔隙率为(76.94±1.08)％,极限抗拉强度为(4.43±0.10)MPa,拉伸弹性模量为(36.08±1.52)MPa,小鼠L929成纤维细胞在其上增殖良好。