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 共查询到17条相似文献,搜索用时 93 毫秒
1.
探讨经京尼平(Genipin)处理的猪主动脉的性能特点和细胞相容性,为构建人工食道提供理想的生物材料。用Genipin处理猪主动脉7min~7天。所获材料进行形态学观察并测定交联指数,同时检测其力学性能、变性温度及体外抗酶解能力;运用人食道上皮细胞研究该材料的细胞相容性。Genipin既有效地去除和降低了组织中产生抗原性的细胞成分和自由氨基,又保持了天然组织构架的完整;经它处理的血管组织具有较好的韧性、黏弹性,适合用作食道的替代材料;Genipin交联材料具有较好的体外抗酶解能力,这有利于其作为移植物植入活体;人食道上皮细胞在本材料上能较好的生长、增殖,材料显示出较好的食道上皮细胞相容性。其中以在pH4.0条件下,浓度为0.625g/100ml,交联7天的材料显示出更好的性能。本材料是一种较为理想的食管替代材料及组织工程食道的支架材料。  相似文献   

2.
探讨经京尼平(Genipin)处理的猪主动脉的性能特点和细胞相容性,为构建人工食道提供理想的生物材料。用Genipin处理猪主动脉7 min~7 d,所获材料进行形态学观察并测定交联指数,同时检测其力学性能、变性温度及体外抗酶解能力,并运用人食道上皮细胞研究该材料的细胞相容性。Genipin既有效地去除和降低了组织中产生抗原性的细胞成分和自由氨基,又保持了天然组织构架的完整;经它处理的血管组织具有较好的韧性、黏弹性,适合用作食道的替代材料;Genipin交联材料具有较好的体外抗酶解能力,这有利于其作为移植物植入活体;人食道上皮细胞在本材料上能较好的生长、增殖,材料显示出较好的食道上皮细胞相容性。其中以在pH4.0条件下,浓度为0.625 g/100 ml,交联7 d的材料显示出更好的性能。  相似文献   

3.
通过盐溶液溶胀和酶液消化制备小肠黏膜下层基质作为血管组织工程支架材料,将大鼠内皮细胞接种于支架材料上,测定小肠黏膜下层基质的细胞相容性.实验结果表明:内皮细胞在小肠黏膜下层基质上迅速铺展增殖生长,在4 h时大约有96%的细胞黏附于材料上,MTT法结果表明内皮细胞在支架上增殖生长明显,活细胞形态学观察表明细胞在支架材料上生长良好.  相似文献   

4.
聚乳酸具有良好的生物相容性以及可降解性能,但其强疏水性能一定程度上限制了其在可降解支架领域的应用。本研究利用氩气(Ar)低温等离子技术在PDLLA血管支架表面接枝亲水性的聚合物聚乙烯吡咯烷酮(PVP)以及聚醋酸乙烯酯(PVAc)。接触角测试表明,经等离子接枝的材料表面接触角由原来的105°~110°可降至22°,由强疏水性变为亲水性。体外细胞粘附性实验表明:等离子处理后的材料表面表现出亲水性质,其细胞的粘附性也得以明显提高,更加有利于细胞在其表面的生长。  相似文献   

5.
针对脱细胞真皮基质(acellular dermal matrix,ADM)孔径小、孔隙率低、孔隙的连通性不理想的难题,以猪中厚皮为原料,综合碱处理、酶处理、去污剂处理和盐处理手段制备了一种支架材料,通过HE染色、红外与紫外可见光谱、扫描电子显微镜、拉力试验、MTT法等手段分析了支架材料的性能。结果显示:支架材料维持了胶原的天然结构,不含细胞成分,紫外与红外吸收符合胶原蛋白的吸收特征,胶原质量分数为(83.19±0.24)%,为具有连通孔道的三维网络结构,孔径约100μm,孔隙率为(76.94±1.08)%,极限抗拉强度为(4.43±0.10)MPa,拉伸弹性模量为(36.08±1.52)MPa,小鼠L929成纤维细胞在其上增殖良好。  相似文献   

6.
为制备仿细胞外基质支架材料,采用静电纺丝技术制备了玉米醇溶蛋白/鼠尾Ⅰ型胶原纳米纤维膜,研究了胶原含量对其纤维直径、亲疏水性、力学性能和生物相容性的影响.结果表明:胶原的最佳添加质量分数为26.7%.胶原的加入能够减小纤维直径,改善其亲水性,且提高其断裂伸长率,有利于更好地模拟细胞外基体组织结构;纤维膜毒性等级为1级,不存在潜在的细胞毒性;接种L929细胞0.5 h后细胞黏附率达60%以上,并且可以促进细胞增殖生长.实验说明该纳米纤维膜具有良好的生物相容性,可作为仿细胞外基质应用于组织工程领域.  相似文献   

7.
将聚乳酸(PLA)短纤纱与聚乙烯醇(PVA)/海藻酸钠(SA)混合水凝胶溶液复合,经冷冻、解冻、浸泡和干燥后获得水凝胶复合纱线,并进一步进行矿化处理。通过扫描电子显微镜(SEM)、能谱(EDS)、红外光谱(FTIR)、吸水率和拉伸测试对试样的形貌结构、元素组成、基团变化、亲水性和力学性能表征分析。结果表明:复合纱线中水凝胶成功披覆纱线表面并渗透入纱线内部,在纱线表面形成三维微孔结构;经矿化处理后,纱线表面沉积大量磷灰石,且磷灰石含量多于纯PLA短纤纱;与纯PLA短纤纱相比,复合纱线吸水率增加约50%,力学性能增强。该制备所得复合纱线具有良好的体外生物活性、吸水性和力学性能,可用于构建组织工程支架材料。  相似文献   

8.
以聚乳酸(PLA)和聚己内酯(PCL)为材料,通过三维(3D)打印和静电纺丝技术,制造组织工程支架,用于培养新生大鼠心肌细胞.将培养了心肌细胞的支架耦合在微电极阵列(MEA)芯片表面构建三维细胞传感器,用于检测心肌细胞的胞外场电位(EFP)信号.实验结果表明,心肌细胞在PLA/PCL支架上附着和生长情况良好,由于兴奋-收缩耦联,能够带动纤维丝产生联合搏动.48h后,支架上心肌细胞的搏动速率趋于稳定.细胞电位检测结果表明,细胞支架与MEA芯片耦合良好,形成三维细胞传感系统,能够检测到支架内心肌细胞的胞外场电位,输出稳定、高信噪比的信号,且EFP信号幅值和发放速率与传统二维培养方法所记录到的信号相似.  相似文献   

9.
血管支架植入后血栓及再狭窄依然是临床上的主要问题。通过材料表面仿生微环境的构建改善支架抗凝性能并加速血管内皮修复是解决上述问题的主要手段。研究表明,VEGF和SDF-1α均可有效介导血管内皮细胞的增殖与迁移。为比较两类因子在内皮化过程中的功能差异,选择在材料表面构建纤连蛋白/肝素功能层,并分别引入VEGF与SDF-1α两种因子,通过XPS和水接触角检测研究修饰表面的理化性质。体外动态释放实验结果显示,修饰层具有良好的控制因子释放的能力。体外细胞相容性评价结果显示,VEGF和SDF-1α均具有刺激内皮细胞增殖和迁移的功能,但VEGF表现出更强烈的调节内皮细胞行为的功能。  相似文献   

10.
作为组织工程中三大要素之一,三维多孔结构支架在组织工程中起着重要的作用。不仅可以为细胞提供生活场所,而且能起到模板作用,引导组织再生和控制组织结构等功能。以1-乙基-(3-二甲基氨基丙基)-碳化二亚胺盐酸盐(EDC)为交联剂,使透明质酸与明胶成功交联,再复合β-TCP微球,制备多孔水凝胶支架。通过X射线衍射(XRD),分析其微球的成分。通过扫描电子显微镜(SEM)和傅里叶红外光谱分析仪(FTIR)对支架进行表征。同时也测定了支架材料的抗压强度、溶胀比、降解性等。为了评估支架的体外细胞相容性,将小鼠L929成纤维细胞接种到支架上用于细胞形态学和细胞活性研究。结果表明,多孔支架的孔径可以通过改变明胶与透明质酸(HA)的比例进行控制,在一定范围内增加透明质酸的比例,孔径会明显增加。随着混合支架中透明质酸比例的增加,溶胀比和降解率也有所增加。支架的抗压强度随明胶比例的增加而增大。一定量的β-TCP能促进细胞的生长与增殖。  相似文献   

11.
In order to investigate the physical mechanism of metal magnetic memory testing, both the influences of earth magnetic field and applied stress on magnetic domain structure were discussed. Static tension and fatigue tests for low carbon steel plate specimens were carried out on hydraulic servo testing machine of MTS810 type and magnetic signals were measured during the processes by the type of EMS-2003 instrument. The results indicate that the initial magnetic signals of specimens are different before loading. The magnetic signals curves are transformed from initial random to regular pattern due to the effect of two types of loads. However, the shape and distribution of magnetic signal curves in the elastic region are different from that of plastic region in tension test. While in fatigue test those magnetic signals curves corresponding to different cycles are similar. The Hp (y) value of magnetic signals on the fracture zone increases dramatically at the breaking transient time and positive-negative magnetic poles occur on the two parts of fracture zone.  相似文献   

12.
在GH635高温合金基材上等离子喷涂NiCrAlY/85Y2O3-ZrO2热障涂层,进行激光重熔,对不同激光工艺参数下的试样进行表面形貌观察并进行热震试验,试验结果表明:采用4.0J/mm^2的激光能量密度可以得到较好的表面质量,并且具有高于等离子喷涂试样的热震寿命。  相似文献   

13.
In order to facilitate self-compacting concrete to be better used in tunnel linings that can resist fires,a SiO_2 aerogel-cement mortar coating was prepared.Based on the HC curve,a self compacting concrete cube specimens coated and uncoated with SiO_2 aerogel-cement mortar (SiO_2-ACM) were heated to simulate tunnel fire for 0.5,1,1.5,2,2.5,3 and 4 h,respectively.The residual compressive strength was tested after the specimens were cooled to room temperature by natural cooling and water cooling.The results show that,the damages of specimens become more serious as fire time goes on,but the residual strength of specimens coated with SiO_2-ACM is always higher than that of uncoated with SiO_2-ACM.In addition,the residual strength of specimens cooled by water cooling is lower than that of natural cooling.However,for the specimens coated with SiO_2-ACM,the adverse effects of water cooling are lessened.With the increase of fire time,the protective effect of SiO_2-ACM is still gradually improved.Finally,a formula was established to predict the residual 150 mm cube compressive strength of specimens protected by SiO_2-ACM after a simulated tunnel fire.  相似文献   

14.
The 60Fc and 70Fc SF/SA blend scaffolds were prepared to mimic the functions of the native ECM for skin regeneration.Human Umbilical Vein Endothelial Cells (HUVECs) were used to examine the cell cytotoxicity,adhesion,growth factors secretion and the gene expression of associated angiogenic factors.Cell proliferation,adhesion and live-dead analyses showed that HUVECs could better attach,grow,and proliferate on the 70Fc scaffolds compared with 60Fc scaffolds and unmodified controls.Furthermore,the 70Fc scaffolds showed higher levels of specific angiogenic proteins and genes expression as well.This study suggests that the involvement of higher composition of SF (about 70%) than that of SA on the blended scaffolds could be advantageous as it is more suitable to promote angiogenesis,which is potential for vascularization during skin repair.  相似文献   

15.
纳米铁/SiO2核壳复合粒子的制备与性能表征   总被引:5,自引:1,他引:5  
利用硅烷偶联剂 APS和活性硅作表面活性剂 ,采用种子包埋法 ,制备了纳米铁 / Si O2 核壳复合粒子。用 TEM、TG- DTA、VSM对材料结构与性能进行表征。TG- DTA表明纳米铁表面没有吸附水和结晶水 ,而抗氧化能力提高。VSM研究发现包裹一层二氧化硅后 ,饱和磁化强度与剩磁对比纳米铁粉减小 ,而矫顽力增大  相似文献   

16.
In order to investigate the physical mechanism of metal magnetic memory testing, both the influences of earth magnetic field and applied stress on magnetic domain structure were discussed. Static tension and fatigue tests for low carbon steel plate specimens were carried out on hydraulic servo testing machine of MTS810 type and magnetic signals were measured during the processes by the type of EMS-2003 instrument. The results indicate that the initial magnetic signals of specimens are different before loading. The magnetic signals curves are transformed from initial random to regular pattern due to the effect of two types of loads. However, the shape and distribution of magnetic signal curves in the elastic region are different from that of plastic region in tension test. While in fatigue test those magnetic signals curves corresponding to different cycles are similar. The H p(y) value of magnetic signals on the fracture zone increases dramatically at the breaking transient time and positive-negative magnetic poles occur on the two parts of fracture zone. Foundation item: Project(50235030) supported by the National Natural Science Foundation of China  相似文献   

17.
Three different kinds of coatings were coated on the concrete surface, and the changes in appearance, surface roughness, microstructure and components of coatings in artificial sewage were investigated. In addition, the strength, micrograph, mineral compositions and pore structure of concrete specimens after removing coatings were also studied. The results show that epoxy coal tar pitch coating(ECTPC) has the best effect of protecting concrete from the sewage corrosion. After being immersed in sewage for 90 days, the compressive strength of concrete coated with ECTPC is still as high as that of specimen immersed in water, and the cement paste has a high CH content and dense structure with low porosity, which mainly accounts for its excellent barrier property and certain antibacterial function. Cement-based bactericidal coating(CBC) also has good effectiveness to sewage corrosion of concrete. The strength and microstructure of concrete coated with CBC in sewage are still significantly superior to those of uncoated concrete. Although cement-based capillary crystalline waterproofing coating(CCCWC) is a good waterproof material, it is not suitable for the corrosion resistance of concrete in sewage. After 2 months corrosion, almost all of the CH crystals in coating reacted with the metabolic acid substance by microbes. Therefore, the strength and pore structure of concrete coated with CCCWC are only slightly superior to those of uncoated concrete. Overall, the protective effect of cement-based inorganic coatings is relatively poor.  相似文献   

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