共查询到20条相似文献，搜索用时 31 毫秒
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干细胞研究现状与展望 总被引：1，自引：0，他引：1
介绍了人胚胎干细胞的建系与定向诱导分化,以及成年组织来源干细胞的多向分化潜能或分化方向的“可塑性”,并提出了干细胞研究的应用前景和存在的主要问题。 相似文献
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脉冲激光沉积(PLD)薄膜技术的研究现状与展望 总被引：2，自引：0，他引：2
综述了脉冲激光沉积 (PLD)薄膜技术的研究现状 ,并按照研究方向将整个研究领域分为三个部分 :PLD法沉积薄膜的机理 ,PLD的工艺研究以及PLD法沉积的主要薄膜材料 ,分别进行了阐述 ,对相关的研究工作提出了建议 ,并对PLD技术的应用前景做了展望 相似文献
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<正>1959年著名诺贝尔物理学奖得主理查德·费曼评论道:"底层有更大的空间"[1],一个科学技术的新世界即将到来。1974年谷口纪男在论述微电子学进展时第一次使用"纳米技术"。20世纪50-70年代,采用微加工技术使微电子器件小型化的研究成果大量涌现,几乎每天都有新颖微型大规模集成电路问世。根据摩尔定律(1965年),每个芯片上的器件数量每24个月增长1倍。从那时起一个纳 相似文献
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<正>(上接7期41页)二、纳米复合材料近10年研究进展2001年一篇综合描述聚合物/填料界面[15]物理和化学特性的文章发表;2002年2篇关于制备和评估纳米介电材料的论文相继独立发表[8-9]。此后,研究者在制备、评估和表征新颖纳米介电材料方面做了大量工作,此领域的第一和第二篇回顾性论文发表于2004年[16-17],2006-2011年又有数篇其他论文相继发表[18-19]。研究表明,界面在确定材料介电性质上起到了重要作用,自组装是制造纳米复合材料的关键工艺。 相似文献
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Anish Hiresha Verma Rupa Haldavnekar Krishnan Venkatakrishnan Bo Tan 《Small Methods》2023,7(1):2200798
Cancer stem cells (CSCs), a rare subpopulation responsible for tumorigenesis and therapeutic resistance, are difficult to characterize and isolate. Conventional method of growing CSCs takes up to 2–8 weeks inhibiting the rate of research. Therefore, rapid reprogramming (RR) of tumor cells into CSCs is crucial to accelerate the stem cell oncology research. The current RR techniques cannot be utilized for CSC RR due to many limitations posed due to isolation requirements resulting in loss of vital data. Hence, a technique that can induce CSC RR without the need for isolation procedures is needed. Here, fabrication of a 3D-silica nanostructured extracellular matrix for RR and in situ monitoring is reported. The RR is tested using three preclinical cancer models. The 3D matrix and a zeta potential study confirm an intense material-cellular interaction resulting in the enhanced expressions of surface and epigenetic biomarkers. Cancer cells require only 3-day period to form CSC spheroids with 3D-silica extracellular matrix. Real-time single-cell monitoring of the methylene blue-induced photodynamic demonstrates the dual functionality. To the authors’ knowledge, this is the first study to demonstrate a CSC epigenetic reprogramming using nanostructures. These findings may pave the path for accelerating the stem cell research in oncology. 相似文献
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Triboelectric Nanogenerator Devices: Triboelectric Nanogenerator Accelerates Highly Efficient Nonviral Direct Conversion and In Vivo Reprogramming of Fibroblasts to Functional Neuronal Cells (Adv. Mater. 34/2016) 
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下载免费PDF全文 Yoonhee Jin Jungmok Seo Jung Seung Lee Sera Shin Hyun‐Ji Park Sungjin Min Eunji Cheong Taeyoon Lee Seung‐Woo Cho 《Advanced materials (Deerfield Beach, Fla.)》2016,28(34):7293-7293
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刘瑛颖;周玉虎;王梓权 《计量科学与技术》2024,68(7):17-22, 48
建立了基于显微成像流式细胞技术对牛奶体细胞数量标准物质中的牛奶体细胞浓度进行测量的方法。该方法通过细胞核荧光染料溴化乙锭（Ethidium Bromide）对牛奶体细胞进行标记,显微成像流式细胞技术提供的单细胞多参数显微图像可有效辅助流式细胞分析方法中对目标细胞的圈门识别,从而提高圈门策略的正确性和重复性。通过多份不同体细胞浓度的牛奶体细胞标准物质样本的多次测量分析证明,该方法具有良好的室内重复性和日间重复性。建立了基于显微成像流式技术的牛奶体细胞数量标准物质中牛奶体细胞浓度测量方法的数学模型,分析确定了该测量方法不确定度主要来源于测量重复性、样本前处理、试剂加样、圈门操作和仪器测量体积偏差等。综合评定,针对研究制备的牛奶体细胞数量标准物质候选物V级（～1.0×106 /mL）,基于显微成像流式细胞技术的牛奶体细胞浓度测量方法的测量相对不确定度为4.84%。 相似文献
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Cellular differentiation, reprogramming and transdifferentiation are determined by underlying gene regulatory networks. Non-adiabatic regulation via slow binding/unbinding to the gene can be important in these cell fate decision-making processes. Based on a stem cell core gene network, we uncovered the stem cell developmental landscape. As the binding/unbinding speed decreases, the landscape topography changes from bistable attractors of stem and differentiated states to more attractors of stem and other different cell states as well as substates. Non-adiabaticity leads to more differentiated cell types and provides a natural explanation for the heterogeneity observed in the experiments. We quantified Waddington landscapes with two possible cell fate decision mechanisms by changing the regulation strength or regulation timescale (non-adiabaticity). Transition rates correlate with landscape topography through barrier heights between different states and quantitatively determine global stability. We found the optimal speeds of these cell fate decision-making processes. We quantified biological paths and predict that differentiation and reprogramming go through an intermediate state (IM1), whereas transdifferentiation goes through another intermediate state (IM2). Some predictions are confirmed by recent experimental studies. 相似文献
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Qing Yu;Xia Li;Juan Wang;Lanping Guo;Luqi Huang;Wenyuan Gao; 《Small (Weinheim an der Bergstrasse, Germany)》2024,20(16):2305708
Photodynamic therapy (PDT) has recently been considered a potential tumor therapy due to its time-space specificity and non-invasive advantages. PDT can not only directly kill tumor cells by using cytotoxic reactive oxygen species but also induce an anti-tumor immune response by causing immunogenic cell death of tumor cells. Although it exhibits a promising prospect in treating tumors, there are still many problems to be solved in its practical application. Tumor hypoxia and immunosuppressive microenvironment seriously affect the efficacy of PDT. The hypoxic and immunosuppressive microenvironment is mainly due to the abnormal vascular matrix around the tumor, its abnormal metabolism, and the influence of various immunosuppressive-related cells and their expressed molecules. Thus, reprogramming the tumor microenvironment (TME) is of great significance for rejuvenating PDT. This article reviews the latest strategies for rejuvenating PDT, from regulating tumor vascular matrix, interfering with tumor cell metabolism, and reprogramming immunosuppressive related cells and factors to reverse tumor hypoxia and immunosuppressive microenvironment. These strategies provide valuable information for a better understanding of the significance of TME in PDT and also guide the development of the next-generation multifunctional nanoplatforms for PDT. 相似文献
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Roberta Visone Giovanni Stefano Ugolini Vladimir Vinarsky Miriam Penati Alberto Redaelli Giancarlo Forte Marco Rasponi 《Advanced Materials Technologies》2019,4(1)
Microfluidic‐based 3D cell culture and organs‐on‐chip have proved able to generate accurate in vitro models of human physiology. Their widespread application and adoption are however hampered by limited scalability and throughput. Here, a novel strategy is described to significantly enhance the throughput of microfluidic systems for 3D cell culture and organs‐on‐chips. A series of 3D culture chambers (up to 96 replicates) can be seeded with a single pipetting operation and a system of normally closed microfluidic valves ensures the resulting 3D microtissues are independent. Devices fabricated with this design principle are employed to perform 3D cultures of rat cardiac fibroblasts and profile two known drugs (doxorubicin, sotalol) in terms of cytotoxicity. In addition, human contractile cardiac microtissues is generated using iPSC‐derived cardiac myocytes and functional assays on microtissues calcium transients after treatment with a known chronotropic drug (verapamil) are performed. The systems here described thus open up new perspective in the scalability of organs‐on‐chip and pave the way to multireplicate 3D cell cultures in microfluidics. 相似文献