Compensation of the inherent wave front curvature in digital holographic coherent microscopy for quantitative phase-contrast imaging

An approach is proposed for removing the wavefront curvature introduced by the microscope imaging objective in digital holography, which otherwise hinders the phase contrast imaging at reconstruction planes. The unwanted curvature is compensated by evaluating a correcting wave front at the hologram plane with no need for knowledge of the optial parameters, focal length of the imaging lens, or distances in the setup. Most importantly it is shown that a correction effect can be obtained at all reconstruction planes. Three different methods have been applied to evaluate the correction wave front and the methods are discussed in detail. The proposed approach is demonstrated by applying digital holography as a method of coherent microscopy for imaging amplitude and phase contrast of microstructures.     

Partially coherent lensfree tomographic microscopy [Invited

Optical sectioning of biological specimens provides detailed volumetric information regarding their internal structure. To provide a complementary approach to existing three-dimensional (3D) microscopy modalities, we have recently demonstrated lensfree optical tomography that offers high-throughput imaging within a compact and simple platform. In this approach, in-line holograms of objects at different angles of partially coherent illumination are recorded using a digital sensor-array, which enables computing pixel super-resolved tomographic images of the specimen. This imaging modality, which forms the focus of this review, offers micrometer-scale 3D resolution over large imaging volumes of, for example, 10-15 mm(3), and can be assembled in light weight and compact architectures. Therefore, lensfree optical tomography might be particularly useful for lab-on-a-chip applications as well as for microscopy needs in resource-limited settings.     

医疗数字X射线成像及其发展趋势——与医用胶片相关的问题

本文概述医疗数字x射线概况及发展趋势。与传统屏片成像相比,数字x射线成像系统有许多优点,包括：射线计量小、图像信息量大、提高诊断质量、减少重拍、影像可通过网络传输、减少冲洗加工污染等,因此得到广泛应用,迅速发展。据统计,2008年全球图像管理与通信系统（PACS）采用率已达64％,几家著名影像工业公司都大力开发数字医疗射线成像相关产品,本文详加介绍。我国的医疗数字X射线成像也在迅速发展,但由于种种原因,完全取代传统的屏片成像还需要较长时间。     

Open-loop band excitation Kelvin probe force microscopy

A multidimensional scanning probe microscopy approach for quantitative, cross-talk free mapping of surface electrostatic properties is demonstrated. Open-loop band excitation Kelvin probe force microscopy (OL BE KPFM) probes the full response-frequency-potential surface at each pixel at standard imaging rates. The subsequent analysis reconstructs work function, tip-surface capacitance gradient and resonant frequency maps, obviating feedback-related artifacts. OL BE KPFM imaging is demonstrated for several materials systems with topographic, potential and combined contrast. This approach combines the features of both frequency and amplitude KPFM and allows complete decoupling of topographic and voltage contributions to the KPFM signal.     

Extended depth of field through an axicon

One of the main disadvantages of imaging systems is the limited depth of field. We present the ability of a refractive axicon for imaging with an extended depth of field. We design an imaging system with a two-step imaging approach that uses a CCD camera to capture intermediate images and uses a digital process to obtain the final images. The depth of field is analyzed based on the condition of the focal segment. In particular, the point spread functions (PSFs) are discussed in simulation in detail. The performed experiments validate the effect and feasibility of the axicon for imaging with an extending depth of field.     

Imaging the surface stress and vibration modes of a microcantilever by laser beam deflection microscopy

There is a need for noninvasive techniques for simultaneous imaging of the stress and vibration mode shapes of nanomechanical systems in the fields of scanning probe microscopy, nanomechanical biological and chemical sensors and the semiconductor industry. Here we show a novel technique that combines a scanning laser, the beam deflection method and digital multifrequency excitation and analysis for simultaneous imaging of the static out-of-plane displacement and the shape of five vibration modes of nanomechanical systems. The out-of-plane resolution is at least 100 pm Hz?1/2 and the lateral resolution, which is determined by the laser spot size, is 1-1.5 μm. The capability of the technique is demonstrated by imaging the residual surface stress of a microcantilever together with the shape of the first 22 vibration modes. The vibration behavior is compared with rigorous finite element simulations. The technique is suitable for major improvements in the imaging of liquids, such as higher bandwidth and enhanced spatial resolution.     

数字化电子影像制作设备

在电视,电影,广告等节目制作中已采用了多种数字化的影像制作设备。本文讨论数字摄像机,数字照相机,虑拟图像制作和数字电影制作等重要设备的基本结构和技术特性,以及使用情况,并探讨它们的未来发展。     

Optimage central organised image quality control including statistics and reporting

Quality control of medical imaging systems is performed using dedicated phantoms. As the imaging systems are more and more digital, adequate image processing methods might help to save evaluation time and to receive objective results. The developed software package OPTIMAGE is focusing on this with a central approach: On one hand, OPTIMAGE provides a framework, which includes functions like database integration, DICOM data sources, multilingual user interface and image processing functionality. On the other hand, the test methods are implemented using modules which are able to process the images automatically for the common imaging systems. The integration of statistics and reporting into this environment is paramount: This is the only way to provide these functions in an interactive, user-friendly way. These features enable the users to discover degradation in performance quickly and document performed measurements easily.     

Superresolution for digital versatile discs (DVD's)

We discuss an extension of our previous research that uses image-plane optical masks to achieve superresolution in confocal scanning microscopy to the coherent optics of digital versatile disc readers. The theory and the design of superresolving optics for such high-numerical-aperture scanning coherent imaging systems is presented. A superresolving optical reader would permit superdense optical data storage, giving an improvement over the equivalent conventional storage densities by at least a factor of 2.     

Digital micromirror device as a spatial illuminator for fluorescence lifetime and hyperspectral imaging

Time-domain fluorescence lifetime imaging (FLIM) and hyper-spectral imaging (HSI) are two advanced microscopy techniques widely used in biological studies. Typically both FLIM and HSI are performed with either a whole-field or raster-scanning approach, which often prove to be technically complex and expensive, requiring the user to accept a compromise among precision, speed, and spatial resolution. We propose the use of a digital micromirror device (DMD) as a spatial illuminator for time-domain FLIM and HSI with a laser diode excitation source. The rather unique features of the DMD allow both random and parallel access to regions of interest (ROIs) on the sample, in a very rapid and repeatable fashion. As a consequence both spectral and lifetime images can be acquired with a precision normally associated with single-point systems but with a high degree of flexibility in their spatial construction. In addition, the DMD system offers a very efficient way of implementing a global analysis approach for FLIM, where average fluorescence decay parameters are first acquired for a ROI and then used as initial estimates in determining their spatial distribution within the ROI. Experimental results obtained on phantoms employing fluorescent dyes clearly show how the DMD method supports both spectral and temporal separation for target identification in HSI and FLIM, respectively.     

Three-dimensional identification of stem cells by computational holographic imaging.

We present an optical imaging system and mathematical algorithms for three-dimensional sensing and identification of stem cells. Data acquisition of stem cells is based on holographic microscopy in the Fresnel domain by illuminating the cells with a laser. In this technique, the holograms of stem cells are optically recorded with an image sensor array interfaced with a computer and three-dimensional images of the stem cells are reconstructed from the Gabor-filtered digital holograms. The Gabor wavelet transformation for feature extraction of the digital hologram is performed to improve the process of identification. The inverse Fresnel transformation of the Gabor-filtered digital hologram is performed to reconstruct the multi-scale three-dimensional images of the stem cells at different depths along the longitudinal direction. For recognition and classification of stem cells, a statistical approach using an empirical cumulative density function is introduced. The experiments indicate that the proposed system can be potentially useful for recognizing and classifying stem cells. To the best of our knowledge, this is the first report on using three-dimensional holographic microscopy for automated identification of stem cells.     

Holographic digital Fourier microscopy for selective imaging of biological tissue

We present an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high‐resolution digital recording, digital Fourier microscopy (DFM) permits crucial flexibility in applying filtering to highlight scatterers of interest in the tissue. The high‐resolution digital hologram is a result of the collation of Fourier holographic frames to form a large‐size composite hologram. It is expected that DFM has an improved signal‐to‐noise ratio as compared to conventional direct digital imaging, e.g., phase microscopy, as applied to imaging of small‐size objects. The demonstration of the Fourier filtering capacity of DFM using a biological phantom represents the main focus of this article. © 2005 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 14, 253–258, 2004; Published online inWiley InterScience (www.interscience.wiley.com). DOI 10.1002/ima.20031     

Object reconstruction from adaptive compressive measurements in feature-specific imaging

Static feature-specific imaging (SFSI), where the measurement basis remains fixed/static during the data measurement process, has been shown to be superior to conventional imaging for reconstruction tasks. Here, we describe an adaptive approach that utilizes past measurements to inform the choice of measurement basis for future measurements in an FSI system, with the goal of maximizing the reconstruction fidelity while employing the fewest measurements. An algorithm to implement this adaptive approach is developed for FSI systems, and the resulting systems are referred to as adaptive FSI (AFSI) systems. A simulation study is used to analyze the performance of the AFSI system for two choices of measurement basis: principal component (PC) and Hadamard. Here, the root mean squared error (RMSE) metric is employed to quantify the reconstruction fidelity. We observe that an AFSI system achieves as much as 30% lower RMSE compared to an SFSI system. The performance improvement of the AFSI systems is verified using an experimental setup employed using a digital micromirror device (DMD) array.     

浅谈传统照相机与数码照相机的差异和共性

本文在研究传统成像系统和数码成像系统的共性和个性的基础上，将数码照相机与传统照相机独有的部件与功能以及两者相近部件与功能列表对比，并加以说明，以简明扼要的方式，表明两者的差异与共性，便于全面理解这两种成像系统的相关知识，对于原来熟悉传统成像技术的专业人员，充分运用原有的理论和实践知识，开展数码成像工作，尤其有帮助。     

Digital holographic microscopy for live cell applications and technical inspection

Digital holographic microscopy enables a quantitative phase contrast metrology that is suitable for the investigation of reflective surfaces as well as for the marker-free analysis of living cells. The digital holographic feature of (subsequent) numerical focus adjustment makes possible applications for multifocus imaging. An overview of digital holographic microscopy methods is described. Applications of digital holographic microscopy are demonstrated by results obtained from livings cells and engineered surfaces.     

Modeling and analysis of soft-test/repair for CCD-based digital X-ray systems

Modern X-ray imaging systems evolve toward digitization for reduced cost, faster time-to-diagnosis, and improved diagnostic confidence. For the digital X-ray systems, charge coupled device (CCD) technology is commonly used to detect and digitize optical X-ray image. This paper presents a novel soft-test/repair approach to overcome the defective pixel problem in CCD-based digital X-ray systems through theoretical modeling and analysis of the test/repair process. There are two possible solutions to cope with the defective pixel problem in CCDs: one is the hard-repair approach and another is the proposed soft-test/repair approach. Hard-repair approach employs a high-yield, expensive reparable CCD to minimize the impact of hard defects on the CCD, which occur in the form of noise propagated through A/D converter to the frame memory. Therefore, less work is needed to filter and correct the image at the end-user level while it maybe exceedingly expensive to practice. On the other hand, the proposed soft-test/repair approach is to detect and tolerate defective pixels at the digitized image level; thereby, it is inexpensive to practice and on-line repair can be done for noninterrupted service. It tests the images to detect the detective pixels and filter noise at the frame memory level and caches them in a flash memory in the controller for future repair. The controller cache keeps accumulating all the noise coordinates and preprocesses the incoming image data from the A/D converter by repairing them. The proposed soft-test/repair approach is particularly devised to facilitate hardware level implementation ultimately for real-time telediagnosis. Parametric simulation results demonstrate the speed and virtual yield enhancement by using the proposed approach; thereby highly reliable, yet inexpensive, soft-test/repair of CCD-based digital X-ray systems can be ultimately realized.     

Psychophysical aspects in medical illumination techniques

The relation between image and visual perception of the human eye is an important point in digital imaging systems. Research aims should therefore pay attention to psychophysical aspects. Optimising of digital imaging systems can only be reached if the important final steps in the diagnostic process--visual perception and signal detection--are taken into account.     

Theoretical foundations for joint digital-optical analysis of electro-optical imaging systems

We describe the mathematical and conceptual foundations for a novel methodology for jointly optimizing the design and analysis of the optics, detector, and digital image processing for imaging systems. Our methodology is based on the end-to-end merit function of predicted average pixel sum-squared error to find the optical and image processing parameters that minimize this merit function. Our approach offers several advantages over the traditional principles of optical design, such as improved imaging performance, expanded operating capabilities, and improved as-built performance.     

X射线数字成像系统及其应用

X射线数字成像技术具有一些引进胶片成像技术所不具备的特点,本文介绍了几种X射线数字成像系统的技术特点和主要性能指标,并就其综合性能进行了比较;介绍了两种常见的X射线数字成像系统的应用情况;对X射线数字成像技术的发展方向作了简述。