共查询到20条相似文献,搜索用时 46 毫秒
1.
用荧光原位杂交(FISH)技术鉴定赤潮甲藻的研究 总被引:6,自引:0,他引:6
探索了用荧光原位杂交(Fluorescence In Situ Hybridization,FISH)技术检测赤潮藻的可行性。用FISH技术鉴定了2株已知的赤潮甲藻——东海原甲藻(Prorocentrum donghaiense)和塔马亚历山大藻(Alexandrium tamarense)。荧光标记的DNA探针是东海原甲藻的核糖体转录单元内间隔区(ITS)序列。研究结果如下:(1)在荧光显微镜下观察与荧光探针杂交后的东海原甲藻细胞,在细胞顶端可见绿色的荧光,而作为对照的塔马亚历山大藻在与标记的探针杂交后没有产生绿色荧光,说明探针只与目标株东海原甲藻反应,不与对照组反应,表明根据ITS序列设计的探针是高度特异的;(2)材料固定后,经蓝光激发,均未产生细胞内源的叶绿素和甲藻素等色素荧光干扰杂交信号的现象,说明固定材料的方法正确。以上的结果表明,FISH技术在鉴定赤潮微藻方面具有其它技术无法比拟的优越性:准确、快速、简单,将其应用到现场定性、定量检测赤潮微藻方面将具有很大的潜力。 相似文献
2.
通过过滤(0.7μm)和切向超滤(100kDa、10kDa、1kDa)技术将实验室内培养的赤潮异弯藻和中肋骨条藻藻液分离成100kDa-0.7μm、10~100kDa、1~10kDa的胶体浓缩液和<1kDa的超滤液,利用激发-发射矩阵荧光光谱技术测定了不同粒级溶液中有机物的荧光性质,分析了三维荧光光谱图中的荧光峰位置、数量及荧光强度的变化情况.结果表明,两种微藻产生的类腐殖质荧光物质主要是<1kDa的小分子物质.赤潮异湾藻产生的类蛋白荧光物质中,15%是100kDa-0.7μm的大胶体,8%是10~100kDa的中胶体,而大部分是<1kDa的小分子物质.赤潮异弯藻各级超滤截留液的类蛋白荧光峰的发射波长与过滤液相比,发生蓝移,而超滤液的则发生红移,表明赤潮异湾藻产生的类蛋白荧光物质随着粒径的增大极性减弱. 相似文献
3.
以rDNA序列为寻找种特异性引物的靶区域,通过分析中肋骨条藻rDNA序列,设计出7套适合用于实时荧光定量PCR方法(RFQ-PCR)的引物与探针,经引物验证,选择Primer6(F/R)进一步分析,对比扩增序列及核酸杂交验证,表明该探针可作为中肋骨条藻特异性探针.最后以Primer6(F/R)和TaqMan6建立了定量检测中肋骨条藻的RFQ-PCR方法,并绘制出了定量检测中肋骨条藻的标准曲线,测定数据用镜检计数进行了验证,证明该方法是准确可靠的. 相似文献
4.
海洋浮游植物生长过程中溶解有机物质的三维荧光光谱研究 总被引:7,自引:1,他引:7
利用三维激发-发射矩阵荧光光谱技术,监测了赤潮异弯藻和中肋骨条藻培养过程中产生的溶解有机物,分析了三维荧光光谱图中的荧光峰位置、数量及荧光强度的变化情况.结果表明,微藻生长过程中会产生类蛋白和类腐殖质两类荧光有机物质,这两类有机物的荧光峰的位置及荧光强度有较大差异.在不同生长期,两类荧光有机物的产生机制不同.在指数生长期,两类有机物的荧光强度与藻密度成正相关,说明浮游植物释放了一定量的荧光物质;在平稳期和衰亡期,两类有机物的荧光强度迅速增加,这可能由于衰老、死亡藻细胞的破碎释放出大量的荧光有机物质所致,此外细菌对非荧光有机物进一步降解,也可能是产生该现象的一个原因. 相似文献
5.
基于量子点的分子灯塔探针的制备及其在DNA探针中的应用 总被引:1,自引:0,他引:1
根据荧光共振能量转移理论合成出一种新颖的分子灯塔探针.由于CdTe量子点(QD s)的荧光发射光谱与DABCYL的紫外-可见吸收光谱有很好的重叠性,所以此种探针采用CdTe量子点作为能量给体,DABCYL作为能量受体.通过水相法合成出直径为2.5 nm的CdTe量子点,并且在偶联剂1-乙基-3-(3-二甲基氨丙基)碳二亚氨盐酸盐(EDC)作用下,与5-′NH2-DNA-DABCYL连接得到了分子灯塔探针.实验发现探针的荧光强度相比CdTe-DNA有明显的下降,最大能量转移效率为68.3%,表明CdTe QD s和DABCYL之间发生了荧光共振能量转移.结果表明,此种探针体系对于互补DNA及其变种有着很好的特异性,且其检测极限为5.170×10^-9mol/L. 相似文献
6.
羟基磷灰石纳米颗粒诱导巨噬细胞凋亡及其与HSP70相互关系的研究 总被引:2,自引:0,他引:2
研究羟基磷灰石纳米颗粒(HAp nanoparticles)引起巨噬细胞凋亡,及其与热休克蛋白Hsp70的相关性.THP-1单核细胞株经PMA诱导成为巨噬细胞,分别加入0、20、100μg/ml HAp纳米颗粒,培养24h后经PI和AnexinV染色,在荧光显微镜下观察,用流式细胞仪检测细胞的凋亡情况,应用western blot 方法检测Hsp70蛋白分子表达的改变.显微镜观察显示THP-1单核细胞株能被诱导成巨噬细胞.荧光显微镜显示20、100μg/ml HAp纳米颗粒组凋亡的阳性信号明显强于0μg/ml对照组;流式细胞仪检测结果也证实HAp纳米颗粒组比对照组凋亡率明显增高(P1<0.0001,P2<0.0001),且凋亡的程度呈浓度依赖性(P3=0.025).Western Blot 检测显示20、100μg/ml HAp纳米颗粒组较对照组Hsp70蛋白表达增加,并且随颗粒浓度增高而表达增加. 相似文献
7.
藻蓝蛋白对Hela细胞CD59基因表达调控作用的研究 总被引:3,自引:0,他引:3
探讨了钝顶螺旋藻藻蓝蛋白(PC)对Hela细胞CD59基因表达的调控作用.以正常人CD59cDNA基因为模板,经PCR扩增后重组入真核表达质粒载体pALTER-MAX,然后利用阳离子脂质体(Lipfectamine-2000)将重组质粒和PcDNA共转染人子宫颈癌细胞(Hela)和对照用正常中国仓鼠卵巢细胞(Chinese hamster ovary,CHO)进行表达.用不同浓度的钝顶螺旋藻藻蓝蛋白作用于转染细胞,通过核酸分子杂交技术、免疫荧光标记法和ELISA法对细胞中CD59分子的表达进行检测.结果表明:成功构建了重组质粒pALTER-MAX-CD59,并将其导入真核细胞(Hela,CHO),经G418筛选获得了CD59分子高效表达的细胞克隆.用藻蓝蛋白作用于筛选出的转基因细胞,证实藻蓝蛋白可促进Hela细胞表面CD59蛋白的表达并抑制Hela细胞的增殖,而对于正常CHO细胞无明显作用. 相似文献
8.
9.
利用流式细胞仪分析了黄海近海水样中聚球蓝细菌(Synechococcus)种群的组成,发现主要由藻红蛋白含量不同的两个类群组成.流式细胞仪分选后,用SN培养基培养纯化得到两株聚球蓝细菌IOCAS0401和IOCAS0402.荧光显微镜下镜检,两株菌在蓝色激发光(450~490nm)下发橘红色荧光,并且IOCAS0401的荧光较IOCAS0402强.扫描电镜观察发现IOCAS0401呈椭圆形,长轴大约1.2μm;IOCAS0402近似球形,直径约0.6μm.吸收光谱的检测表明,两者都有叶绿素a和藻红蛋白的特征吸收峰.其中IOCAS0401有藻尿胆素(PUB)和藻红胆素(PEB)吸收锋,而IOCAS0402只有PEB的吸收峰,两者均无藻蓝蛋白(PC)吸收锋.通过16s rDNA测序分析,结果表明两株菌都位于MC-A中的clade II类群,与从日本海域分离获得的MBIC10224菌株有较高的亲缘关系,虽然这三株菌都归为clade II,但单独成一分支,表明它们带有明显的西太平洋特色. 相似文献
10.
季铵盐壳聚糖纳米荧光探针的制备及细胞渗入研究 总被引:1,自引:0,他引:1
将异硫氰酸荧光素(FITC)接枝到N,N,N-三甲基壳聚糖盐酸盐(TMC)的侧基上,制备了荧光标记的壳聚糖衍生物TMC-g-FITC.其水溶液与羧甲基壳聚糖(CMC)水溶液经凝聚复合,制备了TMC-g-FITC/CMC纳米粒子荧光探针.用激光散射仪和透射电镜表征了该纳米荧光探针的粒径、粒径分布、Zeta电位值和形态结构.结果表明,纳米粒子的粒径在150~360nm范围,粒径分布约为0.10,Zeta电荷值为正值.用MTT法检测了其细胞毒性,发现纳米粒子对细胞的抑制作用较弱,在实验浓度下细胞的抑制率均低于7%.通过激光共聚焦显微镜研究了TMC-g-FITC/CMC纳米粒子荧光探针对HepG2细胞的渗透和迁移过程:纳米粒子先是在细胞周围富集,然后逐渐渗入细胞的内部,最后直至进入细胞核. 相似文献
11.
Permeable crystalline materialcan permeate into pores and cracks of concrete and catalyze the reaction between Ca(OH) 2and unhydrated cement to generate a great quantity needle non-soluble crystals, which can stop up the pores and cracks of concrete, and increase the impermeability of concrete. This paper reported the results of a study conducted to evaluate steel reinforcement corrosion of concrete specimens uncoated and coated with permeable crystalline material as well as mixed with the permeable crystalline material. The properties evaluated for corrosion test were water impermeability, water absorption, compressive strength and potential. The results of water impermeability, water absorption, compressive strength clearly showed that the permeable crystalline material could prohibit water, any soluble salts and moisture from penetrating the concrete to cause corrosion, leaking, and other problems, and it did increase the compressive strength, which was favorable for protection of corrosion of reinforcing steel. Moreover, it was concluded from the potential-time curve that the steel reinforcement of uncoated specimen was in the state of activation whereas that of other specimens coated and mixed with the permeable crystalline material was in the state of inactivation. Above all, it was indicated that the permeable crystalline materialis very effective to protect the steel reinforcement of concrete from corrosion. 相似文献
12.
In this paper, the principle of spurting take off lacquer were introduced; and with orthogonal method the unsaturate polyester was prepared ;it was easy to peel airplane paint layer off with this material and equipment. 相似文献
13.
Sandwich panels utilizing honeycomb cores result in lightweight, high-strength units that are very rigid. Indeed, they give honeycomb panels the best strength-to-weight ratio of any construction type available. Conventionally, the wax is poured into the cell of the core to strengthen the structure and prevent the core from being crushed during milling. Much time and labor is required to wax, mill, and clean the cores. In this study, the process of pouring wax into the core was skipped. In other words, the cutter directly milled the core. Experiments showed that the compressive strength of the core produced without wax stabilization was only 3% lower than that of cores produced using the traditional method. In addition, the drum peel strengths of cores produced without wax stabilization were 38.4% higher than cores produced using the traditional method, in which residual wax seriously polluted the adhesive. It was also found that eliminating the wax process can reduce costs. 相似文献
14.
Muhammad Harris Johan Potgieter Richard Archer 《Materials and Manufacturing Processes》2019,34(6):701-713
Polylactic acid (PLA) is one of the most widely used open source fused filament fabrication materials due to its ease of extrusion, biodegradability, and mechanical strength. The mechanical strength of PLA largely depends on the proper growth of its semi-crystalline structure, which can be severely impaired by a low rate of crystallization, particularly in open source printers. This can be further aggravated by the non-uniform thermal distribution of heat that causes improper curing among the extruded beads of the printing material. As a result, PLA printed on open source printers does not achieve the best mechanical properties. This research, for the first time, proposes an additive-free solution implemented through a detailed set of experimentation to improve the curing rate through in-process temperature variations to cure the joints among the beads. Scanning electron microscopy (SEM), differential scanning calorimetry (DSC) and Fourier transform infrared (FTIR) spectroscopy is used to confirm the improvements in the bead joints. This work is conducted in two phases of experiments. In the first phase, a full factorial ANOVA is used to investigate various process parameters and the important variables are used in the second phase to print test specimens in four different sets. 相似文献
15.
In continuous hot-dip galvanization process the corrosion and chemical stability of the sink roll in the galvanizing bath are important problem which effects on the quality and productivity. In order to protect the sink roll the carbide cermet and/or ceramic coatings were deposited on the surface of the sink roll. The WC-, Cr3 C2-cermet coatings were deposited by high velocity oxygen fuel (HVOF) spray, respectively. The coating samples were immersed in molten Zn-alloy containing 50 wt % aluminum at 833 K for 24 hr and 144 hr, respectively. The inter-diffusion and inter-reaction of Zn, Al and elements in coating and corrosion behaviors of these coatings were investigated by XRD, SEM and EPMA etc. The corrosion mechanisms of the carbide cermet coatings and ceramic coatings in molten High Al-Zn-alloy were approached. 相似文献
16.
Koji Matsumoto Michitaka Morohoshi Yoshikazu Teraoka 《International Journal of Refrigeration》2009,32(6):1336-1342
In many situations, ice often adheres to a cooling solid surface, frequently causing serious accidents. It is critical to clarify the mechanism of ice adhesion to the cooling surface in order to prevent ice adhesion. In a past study, the shearing stresses of two kinds of test plates with a copper surface having the higher thermal conductivity were measured. The shearing stress corresponds to ice adhesion force. Both shearing stresses were significantly different; however, the cause remains unclear.Therefore, the present study focuses on an oxide layer as the main factor causing the difference of both shearing stresses; the influence of the oxide layer formed on shearing stress was discussed. And in the removal and reformation processes of the oxide layer, the time variation of the shearing stress was clarified. Moreover, the relationship between the state of the copper surface and the shearing stress was also clarified by surface analysis. 相似文献
17.
郎志正 《中国标准化(英文版)》2014,(6):86-88
During his inspection in Henan Province in May, Chinese President Xi Jinping stressed to "push forward the transformation from China Speed to China Quality, from Made in China to Created in China, and from Chinese Products to Chinese Brand". The "Three Transformations" are the specific requirements and goal of China's national development in the context that market should play a decisive role in the allocation of resources. 相似文献
18.
正When you are enjoying the green,energy-saving and healthy lighting of IKEA LED luminaire,you may not realize that most of IKEA’s LED bulbs are produced by the LED luminaire smart manufacturing system of Hangzhou Zhongwei Photoelectricity CO.,LTD(ZVISION).No longer enclosed in the laboratory,the product standards are brought into the factory workshop and even to the entire production process.Thus,the standards are promoted and applied in a favorable way by enterprises,benefitting consumers with high quality products. 相似文献
19.
《中国标准化(英文版)》2014,(5):4-4
正The international standard,ISO 8124-6,Safety of toys–Part 6:Certain phthalate esters in toys and children’s products,was officially issued by ISO worldwide on August 15.It was the first time for Chinese experts to lead the ISO standard development in toys industry.The standard was drafted by a group of Chinese experts from SAC/TC for toys and relevant Chinese departments and finally completed by a dedicated international group of experts.ISO 8124-6 makes a substantive breakthrough in the aspects of the scope of toys,limitation of plasticizer types,control of test cost,accuracy of test,operation convenience,etc.,offering the operational guidance for toy manufactures and testing laboratories. 相似文献
20.
《中国标准化(英文版)》2014,(5):6-6
正SAC Administrator Tian Shihong led the Chinese delegation to visit the Spanish Association for Standardization and Certification(AENOR)on September 15,2014.Mrs.Begona Cristeto,the Vice-Minister of Industry,Energy and Tourism of Spain,and Mr.Avelino Brito,the President and CEO of AENOR greeted the delegation.The two sides had a deep and friendly discussion on strengthening the cooperation between China and Spain in the standardization field and promoting the standardization work to support the development of economy and trade in the two countries. 相似文献