Methods for quantifying Staphylococcus aureus in indoor air

Staphylococcus aureus has been detected in indoor air and linked to human infection. Quantifying S. aureus by efficient sampling methods followed by appropriate sample storage treatments is essential to characterize the exposure risk of humans. This laboratory study evaluated the effects of sampler type (all‐glass impinger (AGI‐30), BioSampler, and Andersen one‐stage sampler (Andersen 1‐STG)), collection fluid (deionized water (DW), phosphate‐buffered saline (PBS), and Tween mixture (TM)), and sampling time (3–60 min) on cell recovery. Effects of storage settings on bacterial concentration were also assessed over 48 h. Results showed BioSampler performed better than Andersen 1‐STG and AGI‐30 (P < 0.05) and TM was superior to PBS and DW (P < 0.05). An increase in sampling time negatively affected the recoveries of cells in PBS of BioSampler and AGI‐30 (P < 0.05), whereas cell recoveries in TM were increased at sampling of 6–15 min compared with 3 min. Concentrations of cells collected in PBS were decreased with storage time at 4 and 23°C (P < 0.05), while cells stored in TM showed stable concentrations at 4°C (P > 0.05) and increased cell counts at 23°C (P < 0.05). Overall, sampling by BioSampler with TM followed by sample transportation and storage at 4°C is recommended.     

Molecular comparison of the sampling efficiency of four types of airborne bacterial samplers

In the present study, indoor and outdoor air samples were collected using four types of air samplers often used for airborne bacterial sampling. These air samplers included two solid impactors (BioStage and RCS), one liquid impinger (BioSampler), and one filter sampler with two kinds of filters (a gelatin and a cellulose acetate filter). The collected air samples were further processed to analyze the diversity and abundance of culturable bacteria and total bacteria through standard culture techniques, denaturing gradient gel electrophoresis (DGGE) fingerprinting and quantitative polymerase chain reaction (qPCR) analysis.The DGGE analysis indicated that the air samples collected using the BioStage and RCS samplers have higher culturable bacterial diversity, whereas the samples collected using the BioSampler and the cellulose acetate filter sampler have higher total bacterial diversity. To obtain more information on the sampled bacteria, some gel bands were excised and sequenced. In terms of sampling efficiency, results from the qPCR tests indicated that the collected total bacterial concentration was higher in samples collected using the BioSampler and the cellulose acetate filter sampler.In conclusion, the sampling bias and efficiency of four kinds of air sampling systems were compared in the present study and the two solid impactors were concluded to be comparatively efficient for culturable bacterial sampling, whereas the liquid impactor and the cellulose acetate filter sampler were efficient for total bacterial sampling.     

Impact of culture media and sampling methods on Staphylococcus aureus aerosols

Staphylococcus aureus has been detected indoors and is associated with human infection. Reliable quantification of S. aureus using a sampling technique followed by culture assay helps in assessing the risks of human exposure. The efficiency of five culture media and eight sampling methods in recovering S. aureus aerosols were evaluated. Methods to extract cells from filters were also studied. Tryptic soy agar (TSA) presented greater bacterial recovery than mannitol salt agar (MSA), CHROMagar staph aureus, Chapman stone medium, and Baird–Park agarose (P < 0.05). Moreover, 93 ± 2%–95 ± 2% and 42 ± 1%–49 ± 2% of S. aureus were, respectively, recovered by a 15‐min heating of gelatin filters and 2‐min vortex of polycarbonate (PC) filters. Evaluation of two filtration (IOM with gelatin filter and cassette with PC filter), two impaction (Andersen 1‐STG loaded with TSA and MSA) and four impingement methods [AGI‐30 and BioSampler filled with Tween mixture (TM) and phosphate‐buffered saline (PBS)] revealed the BioSampler/TM performed best over 30 and 60 min of sampling (P < 0.05), while low recovery efficiencies were associated with the IOM/gelatin, cassette/PC, and AGI‐30/PBS combinations (P < 0.05). In addition to BioSampler/TM, collecting S. aureus onto TSA from the Andersen 1‐STG is also recommended, as it is the second best method at the 60‐min sampling (P < 0.05).     

Detection of viable antibiotic‐resistant/sensitive Acinetobacter baumannii in indoor air by propidium monoazide quantitative polymerase chain reaction

Acinetobacter baumannii represents a significant cause of nosocomial infections. Therefore, we combined real‐time quantitative polymerase chain reaction (PCR) with the propidium monoazide (PMA‐qPCR) to assess the feasibility of detecting viable, airborne A. baumannii. The biological collection efficiencies of three samplers for collecting airborne A. baumannii were evaluated by PMA‐qPCR in a chamber study. After sampling, the effects of storage in collection fluid on A. baumannii were evaluated. The results showed that the culturable ratio of A. baumannii measured using the culture method was significantly correlated with the viable ratio measured using PMA‐qPCR, but was not significantly correlated with the qPCR results. It was indicated that the AGI‐30 impinger and the BioSampler were much more effective than the Nuclepore filter sampler for collecting airborne A. baumannii. The storage temperature was critical for aerosol samples, as the loss of viable A. baumannii was minimized when the PMA‐bound DNA was stored at ?20°C or if the collected cells were stored at 4°C and subsequently processed by PMA‐qPCR within 1 month. The PMA‐qPCR method was also to distinguish between colistin‐sensitive and colistin‐resistant A. baumannii, and no colistin‐sensitive A. baumannii was detected by PMA‐qPCR upon treatment of the BioSampler collection medium with 2 μg/ml colistin for 5 min.     

Airborne enteric coliphages and bacteria in sewage treatment plants

The concentrations of airborne culturable microorganisms were determined in wastewater and sludge treatment processes of seven sewage treatment plants. Two types of coliphages, Salmonella and total viable bacteria were sampled by the BioSampler and the numbers of faecal coliforms and enterococci were obtained from the Andersen 6-stage impactor. The BioSampler recovered higher numbers of airborne coliphage viruses than has been measured with other liquid samplers in previous studies, suggesting that this sampler has improved efficiency for sampling airborne coliphages. Airborne coliphages were detected in many stages of the wastewater or sludge treatment process. The highest microbiological air contaminations were found in pre-treatment and aerated grit separation stages of the operation. This was attributed to aerosolisation of microorganisms by mechanical handling or forced aeration. Aeration and settling processes located outdoors caused low microbial concentrations, but the brush aerator released more microorganisms into the air. Our results emphasize the necessity for controlling the exposure of sewage workers to airborne microorganisms, especially in process areas that involve mechanical agitation or forced aeration of wastewater.     

Applicability of the Small Diameter Sampler for Niigata Sand Deposits

Applicability of tube sampling for Niigata sand deposits is discussed through bender element and cyclic triaxial tests for samples obtained from two-chambered hydraulic piston samplers (Shogaki, 1997) with inner diameters of 48 mm and 50 mm, a one-chambered 70 mm diameter sampler, a 125-mm rotary triple-tube sampler and the frozen (FS) sampling method (Yoshimi et al., 1989). The relationship between the relative density (D_r) and normalized SPT N-value (N₁) obtained from small diameter samplers with inner diameters of 45 mm and 50 mm samplers was close to that of the FS and the N₁ coefficient was greater than those of the 70-mm and other tube samplers. The stress ratio at 20 cycles (R_L20) and the initial modulus of rigidity (G_CTX) of samples obtained from the 45-mm and 50-mm samplers were greater than those of the 70-mm, 125-mm rotary triple-tube and other tube samplers. However, the R_L20 values obtained from the 45-mm and 50-mm samplers were smaller than those of the FS sampler in the area of N₁>24. The G_BE and G_CTX values obtained from the 45-mm and 50-mm samplers were close to those of the FS sampling. Therefore, the 45-mm and 50-mm samplers can take equally high quality samples for Niigata sand deposits.     

Effect of a novel temperature‐controlled laminar airflow device on personal breathing zone aeroallergen exposure

Temperature‐controlled laminar airflow improves symptoms in atopic asthmatics, but its effects on personal allergen exposure are unknown. We aimed to evaluate its effects on personal cat allergen and particulate exposures in a simulated bedroom environment. Five healthy volunteers lay under an active and an inactive temperature‐controlled laminar airflow device for 175 min, in a simulated bedroom containing bedding from a cat owner. Total airborne particles (≥0.5 – ≥10 μm diameter) were quantified with a laser particle counter. Airborne allergen was sampled with Institute of Occupational Medicine filters. Inhaled exposure was sampled with nasal air samplers. Allergen‐containing particles were quantified by immunoassay. Treatment reduced total airborne particles (>0.5 μm diameter) by >99% (P < 0.001) and reduced airborne allergen concentration within the breathing zone (ratio of median counts = 30, P = 0.043). Treatment reduced inhaled allergen (ratio of median counts = 7, P = 0.043). Treatment was not associated with a change in airborne allergen concentration outside of the breathing zone (P = 0.160). Temperature‐controlled laminar airflow treatment of individuals in an allergen‐rich experimental environment results in significant reductions in breathing zone allergenic and non‐allergenic particle exposure, and in inhaled cat allergen exposure. These findings may explain the clinical benefits of temperature‐controlled laminar airflow.     

Evaluation of a high-volume portable bioaerosol sampler in laboratory and field environments

This study investigated the physical and biological performances of a portable centrifugal sampler for viable bioaerosols, RCS High Flow. The performance of the test sampler in the laboratory and field environments was compared with that of a reference sampler, BioSampler. The laboratory experiments with non-biological particles of KCl, oleic acid, and polystyrene latex showed that the test sampler's collection efficiency is about 22% for 0.5-microm particles, 48% for 1.0-microm particles, and approximately 100% for particles of 2.5 microm and larger. These tests indicated that the sampler's cut-off size (d50) was 1.1 microm. The test sampler's physical performance when collecting the spores and vegetative cells of Bacillus subtilis var. niger (BG) was similar to that when collecting non-biological particles of the same size. In the laboratory tests, the RCS High Flow sampler was found to enumerate approximately 40% of BG spores and cells relative to the reference sampler, BioSampler. A similar ratio was found during testing in an indoor environment. This ratio decreased to below 10% when testing was performed in an outdoor environment. We hypothesize that the test sampler's underperformance compared with the BioSampler could be caused by the damage to sensitive microorganisms during the collection process, test sampler's sensitivity to wind direction and speed as well as break-up of particle aggregates during the impingement process in BioSampler, which resulted in more colony-forming units (CFUs) being counted by the reference sampler than by the test sampler. Overall, when the RCS High Plus is used to sample culturable airborne microorganisms, the results obtained may have to be adjusted to avoid potential underestimation of microorganism concentration in the air. PRACTICAL IMPLICATIONS: The laboratory testing of the RCS High Flow bioaerosol sampler showed that the sampler collects 1 microm particles and larger with an efficiency of 50% and higher; the efficiency reaches approximately 100% for particles of 2.5 microm and larger. When considering this result, most of the airborne fungal spores would be collected with an efficiency between 50 and 100%. The field testing, however, indicated that the RCS High Flow sampler recovered from 41 to 71% of microorganisms collected relative to the reference sampler, Biosampler, and this ratio dropped to below 5% during outdoor testing. Thus, while the RCS High Flow sampler offers certain advantages over other samplers for viable bioaerosols--it is lightweight, battery operated, and collects viable microorganisms at a high flow rate directly on agar media, the results obtained may have to be adjusted to avoid potential underestimation of microorganism concentration in the air, especially if sampling is performed outdoors.     

Onset and remission of rhinitis among students in relation to the home and school environment—A cohort study from Northern China

Few prospective studies exist on indoor and outdoor air pollution in relation to adolescent rhinitis. We studied associations between onset and remission of rhinitis among junior high school students in relation to the home and school environment. A 2‐year questionnaire cohort study was performed among 1325 students (11‐15 years) in eight schools in Taiyuan, Northern China. Climate and air pollution were measured by direct reading instruments and passive samplers inside and outside the schools at baseline. Associations were calculated by multilevel logistic regression. Two‐year onset of rhinitis and weekly rhinitis were 26.7% and 13.1%, respectively. RH (P < 0.001), CO₂ (P < 0.01) and PM₁₀ (P < 0.01) in the classrooms, PM₁₀ (P < 0.01) and NO₂ (P < 0.05) outside the schools, and redecoration (OR = 2.25) and dampness/indoor mold at home (OR = 2.04) were associated with onset of weekly rhinitis. RH (P < 0.05) and CO₂ (P < 0.05) in the classroom and dampness/indoor mold (OR = 0.67) and environmental tobacco smoke (ETS) at home (OR = 0.63) reduced remission of rhinitis. In conclusion, dampness/mold and chemical emissions from new materials at home can increase onset of rhinitis and ETS and dampness/mold can reduce the remission. PM₁₀, RH, CO₂, and NO₂ at school can increase the onset, and RH and CO₂ can reduce the remission of rhinitis.     

Sample Recovery Ratios and Sampler Penetration Resistance in Tube Sampling for Niigata Sand

In this paper, sample recovery ratios, fluid pressure and sampler penetration resistance in tube sampling are discussed. The samplers were taken by two types of hydraulic piston samplers, one a two-chambered sampler with inner diameters of 45 mm and 50 mm, and the second a one-chambered sampler having a 70 mm diameter. The second type is the one commonly used in Japan and which was also used at the Meike Elementary School, the JGS-1989 investigation site. These samplers are here after referred to as the 45D, 50D and 70S samplers. The mean value of the sample recovery ratios from the 45D and 50D samplers was 93% greater than that of the 70S sampler. This value remained independent of the sands having N = 3 ̴ 54 from the standard penetration test. Guidelines for estimating the pump fluid pressure for tube sampling of sand, sampler and drilling equipment penetration resistance forces and the relationship between the sample recovery ratios and the drilling mud densities, etc., were established for Niigata sand. The end results contribute to a safer and more reliable sampling method.     

Developing a predictive model for fine particulate matter concentrations in low socio‐economic households in Durban,South Africa

In low‐resource settings, there is a need to develop models that can address contributions of household and outdoor sources to population exposures. The aim of the study was to model indoor PM_2.5 using household characteristics, activities, and outdoor sources. Households belonging to participants in the Mother and Child in the Environment (MACE) birth cohort, in Durban, South Africa, were randomly selected. A structured walk‐through identified variables likely to generate PM_2.5. MiniVol samplers were used to monitor PM_2.5 for a period of 24 hours, followed by a post‐activity questionnaire. Factor analysis was used as a variable reduction tool. Levels of PM_2.5 in the south were higher than in the north of the city (P < .05); crowding and dwelling type, household emissions (incense, candles, cooking), and household smoking practices were factors associated with an increase in PM_2.5 levels (P < .05), while room magnitude and natural ventilation factors were associated with a decrease in the PM_2.5 levels (P < .05). A reasonably robust PM_2.5 predictive model was obtained with model R² of 50%. Recognizing the challenges in characterizing exposure in environmental epidemiological studies, particularly in resource‐constrained settings, modeling provides an opportunity to reasonably estimate indoor pollutant levels in unmeasured homes.     

Methodologies for quantifying culturable, viable, and total Legionella pneumophila in indoor air

Legionella pneumophila, aerosolized from numerous indoor facilities (e.g., shower heads, hot tubs, spas), may cause Pontiac fever (PF) and lethal pneumonia named Legionnaires' disease (LD) in humans. Reliable methods on quantitative exposure assessment of this bioaerosol are essential for the prevention of PF and LD. Coupled with culture, ethidium monoazide with qPCR, and qPCR assays, the collection efficiency for culturable, viable, and total L. pneumophila was assessed by means of filtration sampling (IOM with gelatin filter and cassette with polycarbonate filter) and liquid-based sampling methods (BioSampler, AGI-30, MAS-100 sampler with Tween mixture and deionized water (DW)). Results show IOM/gelatin filter was comparable to cassette/polycarbonate filter (P = 0.33) and performed greater than all of tested liquid-based methods for total cell collection. On the other hand, IOM/gelatin filter obtained greater efficiencies than cassette/polycarbonate filter by a factor of 3.8-8.6 for viable cells (P = 0.0006) and two orders of magnitude for culturable cells (P = 0.00002). Further comparison between liquid impingement and filtration methods indicates the sampling by IOM/gelatin filter, AGI-30, and BioSampler with DW were the most appropriate for viable cells, while culturable cells were collected most efficiently by BioSampler/DW with periodical replenishment during the sampling. PRACTICAL IMPLICATIONS: This study recommends the most suitable methodologies for quantifying culturable, viable, and total Legionella pneumophila in indoor air. By using appropriate sampling and analytical methods, the residents and building owners are able to obtain the reliable data and further characterize the exposure risk and/or intervention efficacy against L. pneumophila. Moreover, the adoption of suitable monitoring methods also assists the investigators to explore the sources linked to PF and LD during the outbreaks. Considering reliable microbial monitoring is fundamental for epidemiological survey and risk assessment, the present information should be taken into account in assessing L. pneumophila indoors.     

Building and environmental factors that influence bacterial and fungal loading on air conditioning cooling coils

We investigated bacterial and fungal concentrations on cooling coils of commercial AC units and quantified associations between microbial loads and AC unit or building operational parameters. A field campaign was conducted to sample 25 AC units in the humid, subtropical climate of Southern CT, USA and 15 AC units in the hot‐summer Mediterranean climate of Sacramento, CA, USA. Median concentrations (with interquartile range) of bacteria and fungi on the cooling coils were 1.2 × 10⁷ (5.1 × 10⁶‐3.9 × 10⁷) cells/m² and 7.6 × 10⁵ (5.6 × 10⁴‐4.4 × 10⁶) spore equivalents (SE)/m², respectively. Concentrations varied among units with median unit concentrations ranging three orders of magnitude for bacteria and seven orders of magnitude for fungi. Controlled comparisons and multivariable regressions indicate that dominant factors associated with AC coil loading include the nominal efficiency of upstream filters (P = .008 for bacteria and P < .001 for fungi) and coil moisture, which was reflected in fungal loading differences between top and bottom halves of the AC coils in Southern CT (P = .05) and the dew points of the two climates considered (P = .04). Environmental and building characteristics explained 42% (P < .001) of bacterial concentration variability and 66% (P < .001) of fungal concentration variability among samples.     

Chronic exposure to biomass fuel smoke and markers of endothelial inflammation

Indoor smoke exposure may affect cardiovascular disease (CVD) risk via lung‐mediated inflammation, oxidative stress, and endothelial inflammation. We sought to explore the association between indoor smoke exposure from burning biomass fuels and a selected group of markers for endothelial inflammation. We compared serum concentrations of amyloid A protein, E‐selectin, soluble intercellular adhesion molecule 1 (ICAM‐1) and VCAM‐1, von Willebrand factor (vWF), and high‐sensitivity C‐reactive protein (hs‐CRP) in 228 biomass‐exposed vs. 228 non‐exposed participants living in Puno, Peru. Average age was 56 years (s.d. = 13), average BMI was 26.5 kg/m² (s.d. = 4.4), 48% were male, 59.4% completed high school, and 2% reported a physician diagnosis of CVD. In unadjusted analysis, serum levels of soluble ICAM‐1 (330 vs. 302 ng/ml; P < 0.001), soluble VCAM‐1 (403 vs. 362 ng/ml; P < 0.001), and E‐selectin (54.2 vs. 52.7 ng/ml; P = 0.05) were increased in biomass‐exposed vs. non‐exposed participants, respectively, whereas serum levels of vWF (1148 vs. 1311 mU/ml; P < 0.001) and hs‐CRP (2.56 vs. 3.12 mg/l; P < 0.001) were decreased, respectively. In adjusted analyses, chronic exposure to biomass fuels remained positively associated with serum levels of soluble ICAM‐1 (P = 0.03) and VCAM‐1 (P = 0.05) and E‐selectin (P = 0.05), and remained negatively associated with serum levels of vWF (P = 0.02) and hs‐CRP (P < 0.001). Daily exposure to biomass fuel smoke was associated with important differences in specific biomarkers of endothelial inflammation and may help explain accelerated atherosclerosis among those who are chronically exposed.     

Development and evaluation of an ultrasonic personal aerosol sampler

Assessing personal exposure to air pollution has long proven challenging due to technological limitations posed by the samplers themselves. Historically, wearable aerosol monitors have proven to be expensive, noisy, and burdensome. The objective of this work was to develop a new type of wearable monitor, an ultrasonic personal aerosol sampler (UPAS), to overcome many of the technological limitations in personal exposure assessment. The UPAS is a time‐integrated monitor that features a novel micropump that is virtually silent during operation. A suite of onboard environmental sensors integrated with this pump measure and record mass airflow (0.5–3.0 L/min, accurate within 5%), temperature, pressure, relative humidity, light intensity, and acceleration. Rapid development of the UPAS was made possible through recent advances in low‐cost electronics, open‐source programming platforms, and additive manufacturing for rapid prototyping. Interchangeable cyclone inlets provided a close match to the EPA PM_2.5 mass criterion (within 5%) for device flows at either 1.0 or 2.0 L/min. Battery life varied from 23 to 45 hours depending on sample flow rate and selected filter media. Laboratory tests of the UPAS prototype demonstrate excellent agreement with equivalent federal reference method samplers for gravimetric analysis of PM_2.5 across a broad range of concentrations.     

Airborne fungal cell fragments in homes in relation to total fungal biomass

Fungal exposure may induce respiratory symptoms. The causative agents are compounds in the fungal cell wall. Fragments of microbes may be present in air samples but are not measurable using conventional spore counting or by the determination of viable organisms. This study assesses the proportion of fungal cell biomass and endotoxin in different particle size fractions in air samples from homes. Air samples were collected from 15 homes using a cyclone sampler, collecting particles in three aerodynamic size fractions: <1.0, 1.0–1.8, and >1.8 μm. N‐Acetylhexosaminidase (NAHA) was determined as a marker of fungal cell biomass. Endotoxin was determined using the Limulus amebocyte lysate method. NAHA and endotoxin in the size range <1.0 μm comprised up to 63% (mean 22.7%) and 96.3% (mean 22.6%) of the total concentrations, respectively. There were significant relationships between the amounts of NAHA and endotoxin in the total amount and in the size fraction >1.8 μm but not in the smaller fractions. The results demonstrate significant amounts of fungal cell biomass and endotoxin in particles <1.0 μm. Homes with reported mold damage had a lower concentration of NAHA in particles <1.0 μm than homes without mold damage. To assess airborne exposure for diagnostic and preventive purposes, measurement techniques that include this fraction should be considered.     

Comparison of field performance of the Andersen N6 single stage and the SAS sampler for airborne fungal propagules

Comparisons of two common bioaerosol samplers were made after sampling and enumeration of airborne fungal propagules in several office structures on a university campus in Southern California. Data collected on five occasions throughout the year showed that a Surface Air Systems (SAS) high flow portable sampler recovered consistently lower levels of colony forming units (cfu) than an Andersen N6 single stage impactor. There was no difference statistically between the samplers when concentrations of Cladosporium were compared. Compared to the Andersen N6, the SAS sampler recovered about half the number of cfu for three other fungal categories, i.e. non-sporulating species, Aspergillus and Penicillium and others. Differences in sampler efficiencies are discussed in terms of effective particle diameters. Counts of culturable airborne fungal spores obtained with the SAS sampler should be interpreted with caution when genera other than Cladosporium predominate.     

Coarse particulate matter and airborne endotoxin within wood stove homes

Emissions from indoor biomass burning are a major public health concern in developing areas of the world. Less is known about indoor air quality, particularly airborne endotoxin, in homes burning biomass fuel in residential wood stoves in higher income countries. A filter‐based sampler was used to evaluate wintertime indoor coarse particulate matter (PM_10‐2.5) and airborne endotoxin (EU/m³, EU/mg) concentrations in 50 homes using wood stoves as their primary source of heat in western Montana. We investigated number of residents, number of pets, dampness (humidity), and frequency of wood stove usage as potential predictors of indoor airborne endotoxin concentrations. Two 48‐h sampling events per home revealed a mean winter PM_10‐2.5 concentration (± s.d.) of 12.9 (± 8.6) μg/m³, while PM_2.5 concentrations averaged 32.3 (± 32.6) μg/m³. Endotoxin concentrations measured from PM_10‐2.5 filter samples were 9.2 (± 12.4) EU/m³ and 1010 (± 1524) EU/mg. PM_10‐2.5 and PM_2.5 were significantly correlated in wood stove homes (r = 0.36, P < 0.05). The presence of pets in the homes was associated with PM_10‐2.5 but not with endotoxin concentrations. Importantly, none of the other measured home characteristics was a strong predictor of airborne endotoxin, including frequency of residential wood stove usage.     

Isolation of Aspergillus fumigatus from sputum is associated with elevated airborne levels in homes of patients with asthma

Indoor bioaerosols, such as mold spores, have been associated with respiratory symptoms in patients with asthma; however, dose–response relationships and guidelines on acceptable levels are lacking. Furthermore, a causal link between mold exposure and respiratory infections or asthma remains to be established. The aim of this study was to determine indoor concentrations of Aspergillus fumigatus and a subset of clinically relevant fungi in homes of people with asthma, in relation to markers of airways colonization and sensitization. Air and dust samples were collected from the living room of 58 properties. Fungal concentrations were quantified using mold‐specific quantitative PCR and compared with traditional microscopic analysis of air samples. Isolation of A. fumigatus from sputum was associated with higher airborne concentrations of the fungus in patient homes (P = 0.04), and a similar trend was shown with Aspergillus/Penicillium‐type concentrations analyzed by microscopy (P = 0.058). No association was found between airborne levels of A. fumigatus and sensitization to this fungus, or dustborne levels of A. fumigatus and either isolation from sputum or sensitization. The results of this study suggest that the home environment should be considered as a potential source of fungal exposure, and elevated home levels may predispose people with asthma to airways colonization.     

Modeling microbial growth in carpet dust exposed to diurnal variations in relative humidity using the “Time-of-Wetness” framework

Resuspension of microbes in floor dust and subsequent inhalation by human occupants is an important source of human microbial exposure. Microbes in carpet dust grow at elevated levels of relative humidity, but rates of this growth are not well established, especially under changing conditions. The goal of this study was to model fungal growth in carpet dust based on indoor diurnal variations in relative humidity utilizing the time-of-wetness framework. A chamber study was conducted on carpet and dust collected from 19 homes in Ohio, USA and exposed to varying moisture conditions of 50%, 85%, and 100% relative humidity. Fungal growth followed the two activation regime model, while bacterial growth could not be evaluated using the framework. Collection site was a stronger driver of species composition (P = 0.001, R² = 0.461) than moisture conditions (P = 0.001, R² = 0.021). Maximum moisture condition was associated with species composition within some individual sites (P = 0.001-0.02, R² = 0.1-0.33). Aspergillus, Penicillium, and Wallemia were common fungal genera found among samples at elevated moisture conditions. These findings can inform future studies of associations between dampness/mold in homes and health outcomes and allow for prediction of microbial growth in the indoor environment.