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以大豆分离蛋白为原料,采用超声辅助复合酶酶解制备大豆多肽,以单因素实验为基础,选择复合酶添加量、酶解时间、酶解温度以及酶解p H为自变量,大豆多肽得率为响应值,采用响应面分析法研究各自变量及其交互作用对大豆多肽得率的影响,并对大豆多肽的相对分子质量分布进行测定。结果表明,影响大豆多肽得率的各因素强弱顺序为:酶解温度复合酶添加量酶解时间酶解p H;超声辅助复合酶酶解制备大豆多肽的最佳工艺条件为超声功率180 W、超声时间10 min、超声温度35℃、碱性蛋白酶与中性蛋白酶质量比3∶1、复合酶添加量2.04%、酶解时间4.0 h、酶解温度59℃、酶解p H 8.0,在此条件下大豆多肽得率为63.27%,相对分子质量大部分集中在1 000以下。 相似文献
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为提高大豆分离蛋白(SPI)的水解度,实现深度酶解,生产低分子质量寡肽,比较不同蛋白酶与不同配方的复合酶对SPI的水解能力,并采用响应面法对复合酶的组成进行优化。结果表明:不同蛋白酶对SPI的酶解能力不同,其中以碱性蛋白酶的水解度寡肽收率最高。多酶复合水解可以提高SPI的水解度与寡肽收率,其中以同时加入碱性蛋白酶、风味蛋白酶和中性蛋白酶进行水解时水解度最高。由此3种酶组成的复合酶的最佳组成是碱性蛋白酶39.6%、风味蛋白酶25.4%、中性蛋白酶35.0%,最适用量为SPI干质量的3%。以此复合酶在55℃、SPI质量浓度10g/100mL、自然pH值的条件下酶解6h,SPI的水解度可达27.2%,寡肽收率高达83%。 相似文献
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为提高大豆蛋白的水解度,生产低分子量大豆寡肽,采用碱性蛋白酶、风味蛋白酶和中性蛋白酶对大豆分离蛋白进行水解,并采用中心组合试验和Box-Behnken试验对复合酶的组成和酶解工艺进行了优化。研究结果表明:复合酶的最佳组成为碱性蛋白酶38.4%、风味蛋白酶27.2%、中性蛋白酶34.4%。最佳水解条件为pH 8.79、温度50.46℃、底物浓度10%g/ml,在此条件下酶解6 h,大豆分离蛋白的水解度可达28.7%,寡肽收率高达83.56%,所得大豆寡肽产品中肽含量高达81.3%,数均分子量低至850,各理化指标均符合国标GB/T22492-2008对一级大豆寡肽产品的质量要求。 相似文献
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以低温榨油后的核桃饼粕为原料提取核桃蛋白,采用复合酶水解法制备得到具有降血压活性的血管紧张素转化酶(angiotensin converting enzyme,ACE)抑制肽。在优选复合蛋白酶种类的基础上,以ACE抑制活性为评价指标,通过单因素试验和正交试验进行复合酶酶解参数的优化。结果表明,核桃粕制备ACE抑制肽的最优条件为:复合酶添加质量比1∶2(碱性蛋白酶:中性蛋白酶)、底物浓度4%、酶解温度45℃、pH 8.0、酶添加量8 000 U/g、酶解时间60 min。该条件下制备获得的降血压肽的ACE抑制活性为(63.42±0.70)%,IC50值为(0.838±0.015)mg/mL,多肽相对分子质量主要为0~2 k Da,具有良好的常温贮藏性和消化率。 相似文献
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为优化核桃雄花多肽酶解法制备工艺,该试验以响应面法对核桃雄花多肽制备工艺进行研究。对中性蛋白酶、胃蛋白酶、木瓜蛋白酶、胰蛋白酶、碱性蛋白酶、菠萝蛋白酶和风味蛋白酶进行酶种类筛选及复配,以多肽得率为指标,通过单因素试验及响应面法研究复合酶添加总量、酶解时间、酶解温度、液料比对核桃雄花多肽得率的影响。结果表明,影响核桃雄花蛋白的多肽得率的因素顺序依次为酶解温度>酶解时间>复合酶添加总量>液料比。核桃雄花的最优酶解工艺为液料比8∶1(mL/g)、复合酶添加总量51 500 U/g(木瓜蛋白酶、风味蛋白酶、中性蛋白酶质量比为25∶6∶20)、酶解温度45℃、酶解时间3.0 h,实际多肽得率达到30.00%。 相似文献
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Microgels are ‘soft’ microscopic cross-linked polymeric particles that are being increasingly exploited in a variety of industries for rheology control, encapsulation and targeted delivery. They are valued because of the ability to tune their functionality to address specific applications in oil recovery, coatings, drug delivery, cosmetics, personal care and foods. Food microgels are typically biopolymer hydrogels in the form of microspheres, nanospheres (also called nanogels), spheroids and fibres. The utilisation of engineered microgels in foods has so far been limited, despite their great potential to address several needs in the food industry, including: satiety control, encapsulation of phytonutrients and prebiotics, texture control for healthier food formulations (e.g. reduced fat products), and targeting delivery to specific areas in the digestive tract. We review the scientific and patent literature on the utilisation and manufacturing methods for producing microgels with an emphasis on micro-hydrogels for food applications. 相似文献
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Joubert and Burns prepared a large number of fractions from the high-sulphur proteins of wool and estimated their molecular weights and amino-acid compositions. Their data have been re-examined in order to look for statistically significant interrelations between amino acids and between the proportion of various amino acids and molecular weight. Statistical analysis of the data is also used to examine the credibility of some hypotheses concerning the mechanism of keratin biosynthesis and to provide further evidence for the existence of families of proteins within the high-sulphur fractions of wool. 相似文献
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《肉类研究》2014,(12)
<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of 相似文献
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《印刷工业》2014,(9):94-95
In the 2014 China(Shanghai)International Printing Week,Director Wang Yanbin released the latest data about development of Chinese printing industry in 2013.According to statistics,in 2013,the total output value of Chinese printing industry exceeded 1trillion Yuan for the first time,reaching 1.03985 trillion Yuan.There were 105,000 printing enterprises in China,employees were 3.415 million.The total asset was 1.06247 trillion Yuan; 相似文献
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The State Council Issued "Directory of Government Approved Investment Projects(2013 Edition)" 下载免费PDF全文
《造纸信息》2014,(8)
正On December 2nd,2013,the State Council issued the notification of"Directory of Government Approved Investment Projects(2013 Edition)"(hereafter referred to as"notification").It is pointed out in the"notification"that in order to further deepen reforms in investment systems and administrative examination and approval systems,simplify administrative procedures and delegate powers to lower levels,earnestly 相似文献
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《造纸信息》2014,(8):99-100
On December 24th, 2013, the meeting on the selection of top 10 news of China's paper industry 2013 sponsored by 〈China Paper Newsletters〉 was held in Beijing. The yearly selection of the top l0 news, which began in 2000, has become a brand activity widely recognized in the industry thanks to the support from the authorities at all levels and public participation. 相似文献
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National Development and Reform Commission Issued Announcement for Selection of Major Preliminary Research Projects for the "13~(th) Five-Year Plan" 下载免费PDF全文
《造纸信息》2014,(8):83-84
In Apri Commi major p Plan" (h 2014, the National Development and Reform ssion issued an announcement for selection of reliminary research projects for the "13th Five-Year ereafter referred to as "The Announcement") 相似文献
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《食品科学》2014,(17)
<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the latest scientific 相似文献
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《纺织机械》2014,(3)
正Among the 1600 exhibitors who take apart in the ITMA ASIA+CITME2014 2/3 are Chinese manufactures.If the numerous figures failed to attract your attention,the increase of quality should draw your focus.To adopt the demand of developing textile machine market,domestic textile machinery enterprises now follow the slogan of"technology drives development"to enhance product competitiveness.Our domestic sellers will showcase product ranging from spinning,weaving,dyeing and printing, 相似文献
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A Burgos-Hernández R López-García H Njapau D L Park 《Food Additives & Contaminants》2001,18(9):797-809
In previous studies with aflatoxin-contaminated corn an uncharacteristic response for AFB1 in the Salmonella/microsomal mutagenicity assay (Ames test) was observed and the presence of anti-aflatoxin factors in the corn was suggested. In the current study, corn was extracted and fractionated using thin layer chromatography (TLC) using different developing solvent systems and the Ames test was used to monitor for anti-mutagenic activity in the corn fractions. Both Salmonella tester strains TA98 and TA100 with metabolic activation (S9) were used. Several corn fractions, at different stages in the isolation and purification process, showed anti-mutagenic dose-responses when exposed to pure AFB1. Corn extracts were non-toxic to the tester strains and TLC fractions that showed the best anti-mutagenic dose-responses were selected for further partial characterization analyses. 相似文献