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1.
天然食品防腐剂对酱油防腐效果研究   总被引:1,自引:0,他引:1  
研究采用纳他霉素、乳酸链球菌素、茶多酚这三种天然防腐剂复配用于酱油的防腐保鲜。试验以酱油菌落总数变化为指标,通过单因素分析和正交试验,研究了纳他霉素、乳酸链球菌素、茶多酚在传统酿造酱油中的防腐应用情况,确定了适宜的复合配方。通过对试验的相关数据分析,结果表明:在酱油中单添加其中任何一种防腐剂,只能起到部分抑制酱油中微生物的增殖作用,不能达到对酱油的保鲜效果。三者按合适的比例进行复配,利用它们的抑菌互补特性,在保质期1年内可以有效抑制酱油中微生物的增殖,样品达到我国现行酿造酱油的卫生标准。复合配方的最佳方案有两个,其一是乳酸链球菌素0.2g/kg,纳他霉素0.02g/kg,茶多酚0.1g/kg;其二是乳酸链球菌素0.1g/kg,纳他霉素0.02g/kg,茶多酚0.2g/kg。  相似文献   

2.
乳酸链球菌素与纳他霉素在低盐酱菜中应用的研究   总被引:2,自引:0,他引:2  
以萝卜为原料,进行酱菜制作,重点研究了天然防腐剂乳酸链球菌素(Nisin)与纳他霉素(natamycin)在酱菜加.工中的应用,结果表明,采用天然防腐剂乳酸链球菌素与纳他霉素复配,可有效提高酱菜保藏品质,其最优复合配方为300mg/kg乳酸链球菌素+150mg/kg纳他霉素;乳酸链球菌素与纳他霉素复合防腐剂对酱菜总酸度影响较小:酱菜在保藏3个月内,乳酸链球菌素与纳他霉素复合防腐剂可以改善酱菜的感官品质.  相似文献   

3.
用纳他霉素0.3g/kg,分别与脱氢乙酸0.5g/kg,乳酸链球菌素0.5g/kg,脱乙酰甲壳素(壳聚糖)5g/kg用量搭配混合使用,利用它们的不同抑菌效果,比较和评价纳他霉素和其它三种保鲜剂的作用,结果表明:单独使用纳他霉素对酱卤制品抑菌效果,不如纳他霉素分别与脱氢乙酸、乳酸链球菌素、脱乙酰甲壳素(壳聚糖)协同使用时好。  相似文献   

4.
《中国食品添加剂》2020,(1):113-117
针对复水青豆易失绿、褐变、发臭、品质劣变及货架期短等产业难题,试验以干青豆为试材,重点研究纳他霉素、乳酸链球菌素及其纳他霉素-乳酸链球菌素复合保鲜剂浸泡6h后,常温贮藏期间青豆品质特性变化规律。结果表明,0.02g/kg纳他霉素+0.4g/kg乳酸链球菌素复合保鲜剂浸泡处理,辅以0.03mmPE膜真空包装效果最佳。与对照组相比,常温贮藏货架期延长至第8d,PPO活性和丙二醛含量分别仅为1.9U/g·min和1.22μmol/L,b*值、硬度和pH分别高达27.02、4.6kg/cm2和5.6。试验旨在为复水青豆常温贮运销保鲜提供理论基础和技术支持。  相似文献   

5.
生鲜肉类复合生物防腐保鲜剂的开发研究   总被引:1,自引:0,他引:1  
试验以市售生鲜猪肉作为研究对象,以样品的菌落总数和挥发性盐基氮为评价指标,以乳酸链球菌素、纳他霉素作为主要防腐剂,与EDTA二钠、葡萄糖酸内酯等防腐效果增效剂进行复配试验。通过正交试验,优化获得针对生鲜猪肉的防腐效果较佳的复配保鲜剂配方。最优水平组乳酸链球菌素(0.1875g/kg)+纳他霉素(0.15g/kg)+EDTA二钠(0.125g/kg)+葡萄糖酸内酯(0.05g/kg)。  相似文献   

6.
以传统发酵火腿中筛选得到的乳酸菌、葡萄球菌、埃希氏菌、微球菌、霉菌和酵母菌为研究对象,考察了乳酸链球菌素、聚赖氨酸、脱氢醋酸钠、乳酸钠和纳他霉素对以上腐败菌的抑制作用.在此基础上,探讨了防腐剂复配对火腿中微生物的抑菌效果.结果表明,所采用的防腐剂对火腿中的主要腐败微生物具有较为明显的抑菌作用;当防腐剂复配溶液中乳酸链球菌素、纳他霉素、聚赖氨酸和乳酸的浓度分别为0.1,0.05,0.1 g/L和0.1%(v/v)时,其对火腿中微生物的抑菌率为95.1%.  相似文献   

7.
以豆腐为研究对象,通过添加乳酸链球菌素(nisin)和纳他霉素以提高豆腐的保鲜时间,以感官评分和菌落总数为评价指标,考察了乳酸链球菌素与纳他霉素添加量、杀菌温度和杀菌时间等对其的影响,通过正交试验得出最佳的优化条件为:nisin与纳他霉素添加量0.12 g/kg、杀菌温度90℃和杀菌时间20 min。此工艺条件处理后,冷却并在37℃恒温培养箱中保存7 d,测得试样的感官评分为93分,菌落总数为0.53×10~4CFU/g。没有添加乳酸链球菌素和纳他霉素的对照组在同样条件下保存1 d,测得感官评分在50分以下,这为延长豆腐的保质期提供了实验基础和理论依据。  相似文献   

8.
目的开发一种应用于肉类保鲜的天然、绿色、可食用复合防腐保鲜剂。方法以市售冷鲜猪肉为研究对象,利用普鲁兰多糖成膜的特点,使用纳他霉素、乳酸链球菌素作为主要防腐保鲜剂,柠檬酸作为防腐保鲜增强剂,以色泽、气味、pH、菌落总数和挥发性盐基氮(TVB-N)作为评价指标,优化防腐保鲜剂复配方案。结果最优复配配方为:乳酸链球菌素(0.2 g/L),纳他霉素(0.1 g/L),普鲁兰多糖(5 g/L),柠檬酸(10 g/L)。结论本研究为冷鲜肉防腐保鲜提供了一种经济、安全、有效、实用的配方。  相似文献   

9.
乳酸链球菌素和纳他霉素在月饼保鲜中的应用   总被引:3,自引:0,他引:3  
研究了乳酸链球菌素和纳他霉素对蛋黄月饼的防腐保鲜效果,在蛋黄月饼的蛋黄馅中添加乳酸链球菌素和纳他霉素的复配混合液以及在月饼表皮中添加适量浓度的纳他霉素,具有明显的抑菌保鲜效果,可使蛋黄月饼的保质期延长到45天.  相似文献   

10.
为延长符离集烧鸡的货架期,将乳酸链球菌素(Nisin)、溶菌酶、茶多酚、壳聚糖、纳他霉素和ε-聚赖氨酸等6种生物防腐剂加入到符离集烧鸡中,以菌落总数、挥发性盐基氮、硫代巴比妥酸值和pH值为指标,研究生物防腐剂对符离集烧鸡的保鲜作用。单因素实验结果表明,和壳聚糖、纳他霉素、ε-聚赖氨酸相比,Nisin、溶菌酶、茶多酚3种生物防腐剂具有更好的抑菌效果,并能显著提高符离集烧鸡的品质;在此基础上,采用响应面优化法对3种生物防腐剂进行复配,经分析后建立多元回归模型,确定复合生物防腐剂最优添加浓度为Nisin 0.03 g/kg、茶多酚0.09 g/kg、溶菌酶0.10 g/kg。  相似文献   

11.
Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.  相似文献   

12.
An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.  相似文献   

13.
The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.  相似文献   

14.
The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.  相似文献   

15.
16.
A 9% whey protein (WP) isolate solution at pH 7.0 was heat-denatured at 80°C for 30 min. Size-exclusion HPLC showed that native WP formed soluble aggregates after heat-treatment. Additions of CaCl2 (10–40 mM), NaCl (50–400 mM) or glucono-delta-lactone (GDL, 0.4–2.0%, w/v) or hydrolysis by a protease from Bacillus licheniformis caused gelation of the denatured solution at 45°C. Textural parameters, hardness, adhesiveness, and cohesiveness of the gels so formed changed markedly with concentration of added salts or pH by added GDL. Maximum gel hardness occurred at 200 mM NaCl or pH 4.7. Increasing CaCl2 concentration continuously increased gel hardness. Generally, GDL-induced gels were harder than salt-induced gels, and much harder than the protease-induced gel.  相似文献   

17.
18.
This paper describes the second part of a project undertaken to develop certified mussel reference materials for paralytic shellfish poisoning toxins. In the first part two interlaboratory studies were undertaken to investigate the performance of the analytical methodology for several PSP toxins, in particular saxitoxin and decarbamoyl-saxitoxin in lyophilized mussels, and to set criteria for the acceptance of results to be applied during the certification exercise. Fifteen laboratories participated in this certification study and were asked to measure saxitoxin and decarbamoyl-saxitoxin in rehydrated lyophilized mussel material and in a saxitoxin-enriched mussel material. The participants were allowed to use a method of their choice but with an extraction procedure to be strictly followed. The study included extra experiments to verify the detection limits for both saxitoxin and decarbamoyl-saxitoxin. Most participants (13 of 15) were able to meet all the criteria set for the certification study. Results for saxitoxin.2HCl yielded a certified mass fraction of <0.07 mg/kg in the rehydrated lyophilized mussels. Results obtained for decarbamoyl-saxitoxin.2HCl yielded a certified mass fraction of 1.59+/-0.20 mg/kg. The results for saxitoxin.2HCl in enriched blank mussel yielded a certified mass fraction of 0.48 +/- 0.06 mg/kg. These certified reference materials for paralytic shellfish poisoning toxins in lyophilized mussel material are the first available for laboratories to test their method for accuracy and performance.  相似文献   

19.
Capillary electrophoresis (CE) and polarized light microscopy (PLM) were utilized in the detection of the adulteration of locust bean gum with guar gum. For CE analyses, standards of locust bean and guar gums were extracted with 30% CH3CN, removing the residual proteins from the gum matrix. A 8.75 mM NaH2PO4-20.6 mM Na2B4O7 buffer, pH 9, was used to separate these proteins and to identify marker proteins that were present in the guar gum. These markers did not co-migrate with components in the extracts of mechanically processed locust bean gum, and are used as indicators of adulteration. Using PLM with toluidine blue and iodine staining techniques, unadulterated locust bean gum samples were distinguished from mixed samples through the differential staining of components in locust bean versus guar and tara gums. These experiments in the use of CE and PLM provide orthogonal and complementary methods for the verification of 'true' positives and the elimination of 'false' positives.  相似文献   

20.
BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H2O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.  相似文献   

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