黑曲霉液态发酵生产果胶酶的工艺条件初探

选用鲜苹果渣作为碳源,通过试验探索了黑曲霉液态发酵生产果胶酶的工艺条件。确定最适发酵培基的组成为:麸皮2.5g,鲜苹果渣1.5g,硫酸铵1.0g,K2HPO40.05g,KCl0.025g,MgSO4·7H2O0.025g,Na2SO40.025g,FeSO4·7H20微量,蒸馏水50mL;最佳发酵条件为:发酵时间5d,发酵温度30℃,起始pH2.0,接种量2.0%,装瓶量50mL,在250mL三角瓶中进行摇床振荡培养,最终果胶酶平均酶比活力达到37.91单位/mL。     

白腐菌Trametes versicolor产漆酶发酵条件的优化

对白腐菌Trametesversicolor产漆酶的发酵条件进行了研究,结果表明摇瓶培养产漆酶的最佳培养基组成为:可溶性淀粉2g/L,氯化铵1.2g/L,KH2PO42g/L,MgSO4·7H2O0.5g/L,CaCl2·2H2O0.1g/L,VB10.001g/L,Tween802g/L,微量元素混合液7mL/L,愈创木酚0.015mmol/L,CuSO4·5H2O60μmol/L,pH3.5;最佳发酵条件为:在250mL三角瓶装50mL培养基,接种量(Φ8mm)2块,25℃,150r/min振荡培养10d时,漆酶活力达到862U/L,约是优化前的4倍。     

产过氧化氢酶菌株培养条件的优化

通过对溶壁微球菌培养基优化,确定最佳发酵培养基,并对发酵工艺条件进行了初步的优化.最适碳源是麦芽糖,最适氮源是蛋白胨和酵母膏,最近无机盐是MnSO4;该菌株产酶的最佳培养基配方为:60 g麦芽糖,13 g蛋白胨,8 g酵母膏,1 g MnSO4,5 g NaCl,2 g NaH2 PO4·2H2O,2 g K2HPO4·3H2O,0.5 g MgSO4·7H2O,0.5 g CaCl2,1 000 mL水.从该菌株发酵过程曲线发现,该菌株大量产酶期在12-14 h.摇瓶发酵最适初始pH值为7.0,250 mL三角瓶装液量为25mL,接种体积分数为5%,培养温度为37℃.     

高产果胶酶菌株的筛选鉴定及其发酵条件研究

采用观察透明圈与液态发酵测酶活相结合的方法,从腐烂的水果中筛选获得1株果胶酶产生菌G1。通过形态观察与18S rDNA序列分析,鉴定菌株G1为黑曲霉。对G1菌株的发酵工艺进行优化,确定最佳培养基配方(g/L):桔皮粉32,麸皮10,(NH4)2SO422,Na2HPO4·12H2O 8.4,KH2PO41.4,MgSO4·7H2O 1.0,CaCl20.1,FeSO4·7H2O 0.1,ZnSO4·7H2O 0.1,培养基初始pH 6.5;最佳发酵条件是:装液量50 mL/250 mL,转速180 r/min,接种量9%(体积分数),32℃培养7 d。在此条件下,果胶酶活力达到270.4 U/mL,比优化前提高了8.7倍。     

营养和环境因素对甘油发酵生产鼠李糖的影响

通过正交试验和单因素实验研究各种营养和环境因素对发酵生产鼠李糖的影响。优化后的培养基组成 (g/L)为 :NaNO3 4 0 ,Na2 HPO4 ·12H2 O 3 0 ,KH2 PO4 3 0 ,CaCl2 ·2H2 O0 1,MgSO4 ·7H2 O 0 4 ,酵母膏 2 0 ,微量元素A 2mL/L ,pH6 5 ,甘油 3%。最佳培养条件为 :2 5 0mL三角瓶的装液量为 60mL ,接种量为 10 % ,培养温度为 32～ 36℃。文中认为采用休止细胞培养将有利于产量的进一步提高     

果胶酶生产及其在苹果汁澄清中的应用

对黑曲霉(Aspergillus niger)LLW-1菌株固态发酵生产酸性果胶酶的发酵培养基组成和发酵条件以及利用果胶酶澄清苹果汁的工艺条件进行了研究。固态发酵产酶条件为:250 mL三角瓶装10 g基料(豆饼∶麸皮=2.0 g∶8.0 g),(NH4)2SO420 g/kg,Tween-80 1.5 g/kg,玉米浆50 mL/kg,KH2PO43.0 g/kg,CaCl21.0 g/kg,MgSO4.7H2O 1.0 g/kg(均相对于基料),料水比1∶1.2,自然pH;31℃静置培养72 h,期间在为24 h翻曲1次,果胶酶活力达2 418 IU/g(干曲)。酸性果胶酶澄清苹果汁的工艺条件为:果汁自然pH值,果胶酶用量500IU/L(果汁),明胶添加量0.05 g/L(果汁),酶解温度和时间分别为45℃和1 h。     

多粘类芽孢杆菌20185液态发酵产碱性果胶酶发酵条件的研究

对多粘类芽孢杆菌20185发酵产碱性果胶酶的培养基和发酵培养条件进行了优化.经单因素和正交试验得到最优发酵培养基组成为(g/L):果胶10.0,蛋白胨20.0,K2HPO4·3H2O 6.0,KH2PO4 3.0,NaCl 10.0,MgSO4·7H2O 0.2,SDS 1.0.发酵最佳培养条件为:250mL三角瓶装液量为50mL,种龄24h,接种量9％,初始pH值9.0,温度35℃,培养36h.在此优化条件下,酶活为311U/mL.     

灵芝菌发酵紫甘薯渣获得可溶性膳食纤维的工艺优化

通过单因素和正交实验,优化了以灵芝菌发酵紫甘薯渣生产可溶性膳食纤维的发酵培养基和培养条件,并且进行了发酵罐放大实验。在摇瓶水平,采用紫甘薯渣4 g,豆渣1 g,料液75 mL,pH 6.0,接种量16%,甘蔗渣2%,KH2PO40.1%、MgSO4·7H2O 0.05%、VB10.005%,发酵4天,可溶性膳食纤维达到15.89 g/L。相同条件下,15 L发酵罐中,通气量200 L/h,转速为50 r/min,装液量65%,可溶性膳食纤维达到14.73 g/L。采用优化工艺,发酵前紫甘薯渣中可溶性膳食纤维含量提高了10.92 g/L。     

美味牛肝菌深层发酵条件的优化及成分分析

对美味牛肝菌(Boletus edulis)深层发酵条件及菌丝体主要营养成分进行了分析研究,结果表明,最适培养基配方为:葡萄糖30.0 g,玉米粉20.0 g,酵母膏7.0 g,KH2PO4 2.0 g,MgSO4·7H2O 1.0 g,VB1 0.01 mg,CaCO3 2.0 g;最优发酵条件的初始pH值5.2-5.8,振荡速度180 r／min,培养温度27℃,100 mL三角瓶装液量60 mL.并对美味牛肝菌深层培养菌丝体中蛋白质成分、灰分、脂肪、粗纤维、矿物质及多糖等进行了测定和分析.     

融合子F3产衣康酸的培养基组成及摇瓶发酵条件的优化

对直接利用生淀粉发酵产衣康酸的融合子F3的发酵条件进行了研究。得到较佳培养基组成及发酵条件为:玉米淀粉110g/L,NH_4NO_3 3.0g/L,玉米浆2.5mL/L,MgSO_4·7H_2O1.5g/L,CuSO_4·5H_2O2.0 mg/L,初始pH3.0～3.5,摇瓶装液量80mL/500 mL三角瓶,接种量10%(种龄44h),34℃振荡(220r/min)培养140h。在此条件下,F3衣康酸产率达到45.3g/L,比优化前提高了4.4g/L;对供给淀粉的转化率达41.2%。     

Review of techniques to manufacture micro-hydrogel particles for the food industry and their applications

Microgels are ‘soft’ microscopic cross-linked polymeric particles that are being increasingly exploited in a variety of industries for rheology control, encapsulation and targeted delivery. They are valued because of the ability to tune their functionality to address specific applications in oil recovery, coatings, drug delivery, cosmetics, personal care and foods. Food microgels are typically biopolymer hydrogels in the form of microspheres, nanospheres (also called nanogels), spheroids and fibres. The utilisation of engineered microgels in foods has so far been limited, despite their great potential to address several needs in the food industry, including: satiety control, encapsulation of phytonutrients and prebiotics, texture control for healthier food formulations (e.g. reduced fat products), and targeting delivery to specific areas in the digestive tract. We review the scientific and patent literature on the utilisation and manufacturing methods for producing microgels with an emphasis on micro-hydrogels for food applications.     

12—A CRITIQUE OF SOME HYPOTHESES RELATING TO THE HETEROGENEITY OF THE HIGH-SULPHUR PROTEINS OF WOOL

Joubert and Burns prepared a large number of fractions from the high-sulphur proteins of wool and estimated their molecular weights and amino-acid compositions. Their data have been re-examined in order to look for statistically significant interrelations between amino acids and between the proportion of various amino acids and molecular weight. Statistical analysis of the data is also used to examine the credibility of some hypotheses concerning the mechanism of keratin biosynthesis and to provide further evidence for the existence of families of proteins within the high-sulphur fractions of wool.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of     

The Latest Data about Development of Chinese Printing Industry in 2013

In the 2014 China（Shanghai）International Printing Week,Director Wang Yanbin released the latest data about development of Chinese printing industry in 2013.According to statistics,in 2013,the total output value of Chinese printing industry exceeded 1trillion Yuan for the first time,reaching 1.03985 trillion Yuan.There were 105,000 printing enterprises in China,employees were 3.415 million.The total asset was 1.06247 trillion Yuan;     

The State Council Issued "Directory of Government Approved Investment Projects(2013 Edition)"

正On December 2nd,2013,the State Council issued the notification of"Directory of Government Approved Investment Projects(2013 Edition)"(hereafter referred to as"notification").It is pointed out in the"notification"that in order to further deepen reforms in investment systems and administrative examination and approval systems,simplify administrative procedures and delegate powers to lower levels,earnestly     

China textile machinery: taking off in progress

正Among the 1600 exhibitors who take apart in the ITMA ASIA+CITME2014 2/3 are Chinese manufactures.If the numerous figures failed to attract your attention,the increase of quality should draw your focus.To adopt the demand of developing textile machine market,domestic textile machinery enterprises now follow the slogan of"technology drives development"to enhance product competitiveness.Our domestic sellers will showcase product ranging from spinning,weaving,dyeing and printing,     

Top Ten News of China's Paper Industry 2013

On December 24th, 2013, the meeting on the selection of top 10 news of China＇s paper industry 2013 sponsored by 〈China Paper Newsletters〉 was held in Beijing. The yearly selection of the top l0 news, which began in 2000, has become a brand activity widely recognized in the industry thanks to the support from the authorities at all levels and public participation.     

National Development and Reform Commission Issued Announcement for Selection of Major Preliminary Research Projects for the "13~(th) Five-Year Plan"

In Apri Commi major p Plan＂ （h 2014, the National Development and Reform ssion issued an announcement for selection of reliminary research projects for the ＂13th Five-Year ereafter referred to as ＂The Announcement＂）     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the latest scientific     

Contamination of animal feed and food from animal origin with mineral oil hydrocarbons

Oils and fats used for the production of animal feed can become contaminated with mineral oil material originating from gas oils (C₁₈-C₃₅) or synthetic oils (poly alpha olefins, C₂₅ to beyond C₄₅). An important cause is assumed to be the discharge of waste oils, such as motor oil and hydraulic oils. Mineral oil material was analysed by on-line LC-GC-FID directly in the fat or in a raw extract from animal feed or foodstuffs. In Switzerland in summer/autumn 1999 concentrations in oils and fats for feed production were often found to be between 100 and 1000mg/kg. In the feeds, the average concentration was around 100mg/kg with values ranging up to a maximum of 1000mg/kg; few samples were free of contamination. In animal body fat, the average concentration determined in summer 1999 was 25mg/kg, with a maximum of 150mg/kg, although in samples from December 1999, contamination was substantially lower. In the fat phase of eggs, the average concentration was 30mg/kg, with a maximum of 80mg/kg. Paraffin oil is used for feed production, which may account for part of the contamination problem (e.g. eggs).