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1.
番茄红素检测方法的建立   总被引:5,自引:0,他引:5  
侯纯明  何美  周鑫 《食品科学》2007,28(5):295-298
本实验从番茄红素及β-胡萝卜素的紫外图谱差异入手,确定了以氯仿为溶剂,以518.8nm吸收峰为测定波长的番茄红素测定方法,避免了其它类胡萝卜素的干扰。  相似文献   

2.
紫外分离测定番茄红素   总被引:4,自引:0,他引:4  
从番茄红素及β-胡萝卜素的紫外图谱差异入手,确定了以氯仿为溶剂.以518.8I瑚吸收峰为测定波长的番茄红素测定方法,避免了其它类胡萝卜素的干扰。  相似文献   

3.
番茄红素测定方法的研究   总被引:10,自引:0,他引:10  
番茄红素是一种具有多种生理功能,但又不能为人体自身合成的天然类胡萝卜素,目前已被用作一些功能性食品的主要成分,但是其测定方法的不一致,给研究工作带来了一定困难。本文对HPLC法和分光光度法测定番茄酱中番茄红素的含量进行了比较,探讨了准确、简便测定番茄红素的方法。  相似文献   

4.
番茄红素及生产应用研究   总被引:14,自引:1,他引:13  
番茄红素是开链式的不饱和类胡萝卜素,具有抗氧化、清除自由基、促进细胞间的连接和传导、防癌抗癌等多种生理功能,作为一种功能性天然色素,番茄红素被认为可以广泛利用在功能性食品和医药(原料)中。本文详细介绍了番茄红素的分布、提取工艺、生理功能、主要应用及国内外番茄红素的研究和生产情况。  相似文献   

5.
番茄红素提取工艺的优化研究   总被引:31,自引:0,他引:31  
番茄红素是番茄及番茄制品中的主要类胡萝卜素,同时也是人体血浆和组织中的主要类胡萝卜素。它是一种天然色素而且具有优越的生理功能和保健作用。该研究以番茄原浆、市售番茄酱及市售新鲜番茄为原料,经预处理后,采用正己烷、石油醚、石油醚-丙酮等有机溶剂浸提,将番茄红素提取出来。经过对比实验,确定以番茄原浆为原料的最佳萃取工艺条件,通过紫外及可见光谱分析确定所提取产品为番茄红素。  相似文献   

6.
番茄红素为一种天然类胡萝卜素,具有抗氧化、延缓衰老、防癌抗癌、预防心脑血管疾病、增强免疫力、保护皮肤等多种功能活性,在食品、医药和饲料行业得到广泛应用。综述近年来番茄红素提取技术及其功能活性在我国的研究进展,为番茄红素的进一步研究开发提供参考。  相似文献   

7.
番茄红素是一种天然类胡萝卜素,具有抗氧化、延缓衰老、抑制肿瘤、降低血脂、提高人体免疫力、预防心血管疾病、延缓骨质疏松等多种功效。本文综述了近五年番茄红素药理作用的研究进展,为新型药物的开发和应用提供依据。  相似文献   

8.
番茄红素的生理保健功能及应用研究进展   总被引:2,自引:0,他引:2  
番茄红素是一种天然的类胡萝卜素,对人体具有多种生理保健功能,是目前国际上食品、保健品和化妆品等领域研究的热点。本文综述了番茄红素生理保健功能的作用机制,及产品应用的进展情况,并展望其发展前景。  相似文献   

9.
国内外番茄红素的研究与生产现状   总被引:8,自引:0,他引:8  
番茄红素是开链式的不饱和类胡萝卜素,具有抗氧化,清除自由基,促进细胞间的连续与传导,防癌抗癌等多种生理功能,作为一种功能性天然色素,番茄红素被广泛地用于多种功能性食品。本详细介绍了国内外番茄红素的研究与生产情况。  相似文献   

10.
番茄红素--具有保健功能的食用色素   总被引:4,自引:0,他引:4  
番茄红素属于不具有环状结构的一种独特的类胡萝卜素,具有很强的抗氧化活性和多种生理功能,成为保健食品研究的热点。本文从多个方面介绍了番茄红素对人体的保健功能。  相似文献   

11.
运用电子顺磁共振波谱(EPR),通过三种类胡萝卜素(番茄红素、叶黄素、β-胡萝卜素)对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和羟基自由基(·OH)清除能力大小的比较,测定了三种类胡萝卜素抗氧化活性能力。结果表明,DPPH和Fe2+(固定Fe2+:H2O2=1:10)浓度均与EPR信号呈良好的线性关系,相关系数R2均大于0.99。三种类胡萝卜素对DPPH·和·OH均有一定清除作用,且随浓度的增大和时间的延长清除作用逐渐增强。其中,番茄红素的抗氧化活性能力最强,叶黄素次之,β-胡萝卜素最弱,浓度为0.6 mmol/L的番茄红素、叶黄素和β-胡萝卜素对DPPH·的清除率分别为95.4%、76.7%和47.7%,对·OH的清除率分别为56.6%、29.1%和14.0%。相较于紫外-可见分光光度(UV-Vis)法,该方法可以有效避免类胡萝卜素自身颜色对测试结果的干扰,研究结果可为完善类胡萝卜素等天然食品添加剂的抗氧化能力评价方法提供依据。  相似文献   

12.
建立了番茄制品及膳食补充剂中的番茄红素含量的超高效液相色谱分析方法,采用ACQUITY UPLC C18色谱柱(1.7μm×2.1cm,50mm),流动相为乙酸乙酯和乙腈梯度洗脱,二极管阵列检测器,检测波长为472nm,流速0.3mL/min,柱温35℃.在此条件下,3min内能够将番茄红素与β-胡萝卜素完全分离,而且番茄红素线性关系良好,相关系数0.9991;平均回收率为88.1%~96.1%;检出限为0.01μg/mL.方法简便、快速、准确,可用于各种食品中番茄红素检测.  相似文献   

13.
本实验旨在研究来自北极海洋红球菌B7740类胡萝卜素和类异戊二烯醌提取物(B7CIQE)的体外抗氧化活性(通过β-胡萝卜素漂白测定、脂质和蛋白质氧化抑制实验、DNA氧化断裂抑制实验)、抗增殖活性(通过抗人肝癌细胞Hep G2和人口腔癌细胞KB增殖实验)和细胞内抗氧化效果。实验中使用日常饮食常见高等植物来源类胡萝卜素(β-胡萝卜素、叶黄素、番茄红素)作为对照组,评价B7CIQE的生物活性:β-胡萝卜素氧化抑制率为B7CIQE(70.20%)2,6-二叔丁基-4-甲基苯酚(66.70%)表没食子儿茶素没食子酸酯(17.80%)番茄红素(1.90%);油脂开始氧化的温度顺序为B7CIQE(175℃)β-胡萝卜素(165℃)叶黄素(162℃)番茄红素(160℃);蛋白质氧化抑制率为B7CIQE(25.75%)β-胡萝卜素(24.97%)叶黄素(17.94%)番茄红素(10.40%);Hep G2细胞抗增殖实验半最大效应浓度为叶黄素(20.86μg/m L)β-胡萝卜素(124.88μg/m L)B7CIQE(126.34μg/m L)番茄红素(139.24μg/m L);KB细胞抗增殖实验半最大效应浓度为B7CIQE(25.14μg/m L)叶黄素(64.29μg/m L)番茄红素(69.87μg/m L)β-胡萝卜素(149.16μg/m L)。结果表明,与植物源类胡萝卜素相比,B7CIQE具有相对更优异的抗氧化和抗增殖活性。  相似文献   

14.
A rapid, isocratic RP-HPLC method for the determination of the carotenoids produced by Blakeslea trispora is described. The mixture of acetone:acetonitrile, 60:40, v/v, found appropriate for the cellular triacylglycerol analysis, was also successfully used for the separation of lycopene, γ-carotene and β-carotene. The method was validated for β-carotene using an olive oil triacylglycerol fraction devoid of carotenoids. Recovery study (300 mg/kg oil) was 99%. RSDr and RSDL were satisfactory (<4%). The limit of quantification was found to be 4.56 ng/5 μL and the system was linear in the range 2.0–30.0 ng/μL.  相似文献   

15.
Four major carotenoids of high nutritional significance, including β-carotene, lycopene, lutein and zeaxanthin were determined in three isolates of heterocystous cyanobacteria, belonging to the genera Anabaena and Nostoc, isolated from Iranian terrestrial and aquatic ecosystems, for the first time. The ultrasonically extracted carotenoids were identified and quantified by a rapid and sensitive isocratic HPLC method and identification was further confirmed by spiking authentic standards and the pattern of the UV–Vis spectra obtained from photo-diode array detector. The results showed that these isolates contain large amounts of four major carotenoids, especially lycopene (up to 24570 μg/g dry weight, DW) which appears to be the highest reported amount until present; and β-carotene (up to 8133 μg/g DW) which is comparable with the best natural sources of β-carotene. Meanwhile, they are rich in the cis-isomers of lycopene and β-carotene which is important in their bioavailability and health benefits.  相似文献   

16.
An in vitro cell culture model to assess the intestinal absorption of carotenoids is described. When supplemented with taurocholate and oleic acid, differentiated Caco-2 cells on membranes are able to produce chylomicrons. Under conditions mimicking the in vivo postprandial state, Caco-2 cells take up carotenoids and secrete them incorporated into chylomicrons; the extents of absorption of all-trans, 9-cis, 13-cis β-carotene, α-carotene, lutein, lycopene were 11%, 2%, 3%, 10%, 7%, and 3%, respectively. Saturation of β-carotene transport through Caco-2 cells occurred at concentrations (>15 μM) higher than “physiological” concentrations. Finally, retinol supplementation resulted in an 1.7-fold increase in β-carotene transport. The data suggest that (1) the intestinal absorption of carotenoids is facilitated by the participation of a specific epithelial transporter and (2) retinol promotes β-carotene incorporation into larger, retinyl ester-enriched chylomicrons. The present in vitro cell culture system is a relevant model to study the intestinal absorption of carotenoids at the molecular level.  相似文献   

17.
This study determined the carotenoids content in cherry tomato, pink guava, and red grapefruit pulps and juices. Cherry tomato pulp exhibited the highest β-carotene content whereas pink guava pulp had the highest lycopene content. However, β-carotene and lycopene contents in the studied fruit juices were lower than their pulps in the same sample portion. Interestingly, six to twelve cis-isomers of carotenoids were identified in the fruit pulps and juices studied. A higher number of trace amounts of cis-carotenoids was found in fruit pulps as compared to juices. Therefore, consumption of whole fruit is recommended as the studied fruit juices have lower carotenoids content.  相似文献   

18.
Major antioxidants and antioxidant activity in eight varieties of tomatoes were determined. Hydrophilic and lipophilic antioxidant activity (HAA and LAA) was determined by the ABTS assay and ascorbic acid and carotenoid contents were determined by HPLC-DAD. The HAA has far more significant impact on total antioxidant activity (83%) as compared with LAA. HAA was increasing during all ripening stages and was strongly correlated with ascorbic acid content (r = 0.83). During the ripening the LAA was increasing till the III. stage of maturity and then decreased. The main carotenoids determined in the red-fruit tomatoes were lycopene, β-carotene and lutein. The content of lycopene has been increasing equally during the ripening. β-Carotene and lutein were intensively synthesized between the I. and II. stage of maturity. Among ascorbic acid, α-tocopherol, lycopene, β-carotene and lutein standards ascorbic acid was determined as the most efficient antioxidant followed by α-tocopherol and β-carotene. Antioxidant activity of ascorbic acid, α-tocopherol, β-carotene and lutein grew equally with increasing concentration, however lycopene was the most effective in its lowest concentration. The analysis of two-component mixtures showed significant synergism between lycopene-lutein, lycopene-β-carotene and α-tocopherol-β-carotene.  相似文献   

19.
从番茄提取番茄红素与β-胡萝卜素的工艺研究   总被引:1,自引:0,他引:1  
研究从番茄中提取、分离番茄红素与β-胡萝卜素的工艺技术。结果表明:在丙酮、石油醚、正己烷、乙酸乙酯4种溶剂中,丙酮对β-胡萝卜素和番茄红素的提取率最高。丙酮提取的次数以3次较为适宜。用中性氧化铝柱色谱,依次用石油醚与氯仿洗脱,可分离β-胡萝卜素与番茄红素。丙酮提取番茄类胡萝卜素的适宜工艺条件为:温度60℃、时间4h、pH值5、料液比1:5。  相似文献   

20.
A carotene extract from the fruits of the oil palm (Elaeis guineensis) was analysed by HPLC employing a C30 column for better separation efficiency. A multitude of cis-isomers of α-, β- and γ-carotene were separated. Detailed assignment was possible by subjecting pure standards of α-, β- and γ-carotene to isomerisation and comparing spectral data and order of elution to literature data. α- and β-carotene were found to be the most abundant carotenoids comprising 12.3% and 17.9%, respectively, of a (roughly) 30% oil suspension of oil palm carotenes in vegetable oil. A large proportion (about 40%) of α- and β-carotene was in the form of cis-isomers. The γ-carotene content was found to be 0.38% and other carotenes like phytoene, phytofluene, ζ-carotene, lycopene and possibly β-zeacarotene were found as well but were not quantified.  相似文献   

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