红酵母产生类胡萝卜素的研究

研究了红酵母RY-98菌株产生类胡萝卜素的营养与环境条件,并对发酵过程生理现象的变化作了测试.结果表明培养基组成、糖浓度和添加剂以及培养基初始pH值、通气量(摇瓶装量)与培养时间等,均对该菌细胞生物量和类胡萝卜素产量有影响.其中ZS添加剂的加入可以明显促进菌体类胡萝卜素的形成,这是本次研究的新发现.在初步优化的培养基组成(葡萄糖400g/L、玉米浆15g/L、(NH4)2SO42g/L、ZS添加剂1g/L)和培养条件下,菌体生物量与类胡萝卜素含量分别为27mg/mL和498.2μg/g,发酵液产色素能力为13.3mg/L.通过观测发酵过程生理现象的变化,认为该菌类胡萝卜素是菌体的次生代谢产物,主要形成于对数生长期的末期和稳定期.     

红酵母产生类胡萝卜素的研究注

研究了红酵母RY－98菌株产生类胡萝卜素的营养与环境条件，并对发酵过程生理现象的变化作了测试。结果表明：培养基组成、糖浓度和添加剂以及培养基初始pH值、通气量（摇瓶装量）与培养时间等，均对该菌细胞生物量和类胡萝卜素产量有影响，其中ZS添加剂的加入可以明显促进菌体类胡萝卜素的形成，这是本次研究的新发现，在初步优化的培养基组成（葡萄糖40g/L、玉米浆15g/L、（NH4）2SO42g/L、ZS添加剂1g/L）和培养条件下，菌体生物量与类胡萝卜素含量分别为27mg/mL和498.2μg/g，发酵液产色素能力为13.3mg/L。通过观测发酵过程生理现象的变化，认为该菌类胡萝卜素是菌体的次生代谢产物，主要形成于对数生长期的末期和稳定期。     

类胡萝卜素产生菌的筛选及培养条件初步研究

从酒糟中分离出的一株产类胡萝卜素的细茼（TH），分类鉴定为微球菌属（Micrococcus sp.）。该菌株稳定性较好，在以葡萄糖为碳源（5g／L），添加番茄汁（4mL／L），Na2CO3（3g／L），初始pH值7．0，光照的条件下于28℃振荡培养120h，并以酸-热破碎法进行色素抽提。菌体生物量为55．04mg／mL培养液，类胡萝卜素产量为7．12μg／mL培养液。     

海洋红酵母Rhodotorula sp.06产类胡萝卜素的研究

对海洋红酵母Rhodotorula sp.06产类胡萝卜素的最优条件进行研究,比较不同碳源、氮源、NaCl、装液量、培养时间对菌体生物量和色素形成的影响,通过正交实验确定最佳摇瓶发酵条件组合。结果表明,海洋红酵母的最佳摇瓶发酵条件为:蔗糖15g/L、蛋白胨20g/L、NaCl20g/L、NH4Cl7.5g/L、24℃、装液量80/250mL、发酵时间72h。在此最优条件下,其生物量、类胡萝卜素含量和产量分别为10.27g/L、296.11μg/g、3.04mg/L。     

红酵母RY-08产类胡萝卜素的发酵条件研究

研究不同碳源和氮源对红酵母RY-08产类胡萝卜素的影响,并用正交试验对碳源、氮源、装液量及发酵时间进行优化。结果表明在葡萄糖40 g/L,蛋白胨20 g/L,装液量30 mL/250 mL,发酵时间72 h的发酵条件下其生物量、类胡萝卜素含量和产量分别达到19.7 g/L,418.6μg/g,8.2 mg/L。     

红酵母类胡萝卜素发酵研究

对红酵母 RY-98菌株产生类胡萝卜素的培养基组成和培养条件进行了初步研究 ,并对发酵过程作了动态分析 .结果表明 :培养基组成、糖浓度和添加剂以及培养基初始 p H值、摇瓶装量与培养时间等 ,均对该菌细胞生物量和类胡萝卜素产量有影响 ,其中 ZS添加剂的加入可以明显促进菌体类胡萝卜素的形成 .在初步优化的培养基组成 (葡萄糖 4 0 g/L,玉米浆 1 5g/L,( NH4) 2 SO42 g/L,ZS添加剂 1 g/L)和培养条件 (培养基初始 p H6.0 ,装量 60 m L/2 50m L摇瓶 ,2 8℃培养 60 h)下 ,菌体生物量及类胡萝卜素含量分别为 2 7mg/m L和 4 98.2 μg/g.通过观测发酵过程生理现象的变化 ,确认该菌类胡萝卜素是菌体的次生代谢产物 ,主要形成于对数生长期的末期和稳定期 .     

光合细菌ZY2159菌株发酵生产类胡萝卜素研究

本研究探讨了发酵培养条件对红杆菌类胡萝卜素突变株ZY2159菌株细胞生长与类胡萝卜素生物合成的影响。结果表明,在温度30℃,光照2000lux,接种量10%,70%装样量,Fe^3＋为15mg/L条件下,在5L发酵罐培养ZY2159菌株6d可以获得最大生物量和最高类胡萝卜素生物合成量,菌体得率为15.4g/L,类胡萝卜素产量达50.9mg/L。HPLC色素分析显示,该菌株产β-胡萝卜素,占类胡萝卜素总量的22.3%。     

锁掷酵母高产类胡萝卜素的发酵条件的研究

分析了锁掷酵母中类胡萝卜素的主要代谢途径,研究了碳源、温度、pH、溶氧、有机氮源对锁掷酵母发酵生产类胡萝卜素的影响,发现碳源、温度、pH主要影响菌体的得率;溶氧、有机氮源主要影响类胡萝卜素的组成。可以通过最适的发酵条件单一积累目的类胡萝卜素。以玉米浆为氮源、溶氧控制在5%左右有利于β-胡萝卜素的积累,在优化的条件下,经7L罐补料分批发酵,菌体得率为56.32g/L,β-胡萝卜素产量可达18.92mg/g,占总类胡萝卜素量的60.43%,比优化前提高了217%;当以酵母膏为氮源、溶氧控制在30%左右有利于红酵母烯的积累,在优化的条件下,经7L罐补料分批发酵,菌体得率为62.47g/L,红酵母烯的产量可达31.74mg/g,占总类胡萝卜素的70.41%,比优化前提高了152%。通过优化发酵条件来定向积累单一的类胡萝卜素可为其他菌株生产类胡萝卜素提供一种可行的方法。     

荚膜红假单胞菌产类胡萝卜素的培养基优化实验

对荚膜红假单胞菌产类胡萝卜素的培养基组成进行优化研究,结果表明,荚膜红假单胞菌产类胡萝卜素的最优培养基组成为:柠檬酸钠3·9g、NH4Cl0·4g、KH2PO41·36g、酵母膏0·1g、无水MgSO40·3g、FeSO49mg、蒸馏水1000mL,pH7·4。该配方在30℃,500lx光照下,类胡萝卜素的产量为1·07mg/g菌体湿重,较优化前产量的0·45mg/g(菌体湿重)提高了1倍多,而培养基成分减少了6种。     

菌体蛋白水解液应用于谷氨酸发酵的研究

谷氨酸菌体蛋白来自温敏型谷氨酸发酵生产废渣,经水解后替代适量豆粕水解液用于温敏型谷氨酸发酵培养基中。通过进行发酵工艺优化,实验结果表明在温敏型谷氨酸发酵培养基中添加一定量的谷氨酸菌体蛋白水解液,发酵的产酸和转化率有显著的提高。经过工艺优化,温敏型谷氨酸发酵培养基组成确定为:淀粉水解糖55g/L,玉米浆20mL/L,谷氨酸菌体蛋白水解液10g/L,豆粕水解液10g/L,糖蜜15g/L,Na2HPO47g/L,KCl 4g/L,MgSO4·7H2O 1.5g/L,MnSO4·7H2O 30mg/L,FeSO4·7H2O 30mg/L,维生素B1350μg/L,维生素H 500μg/L,消泡剂0.1mL/L。在此培养条件下,谷氨酸的产酸率达18g/L,转化率达65%,温敏型谷氨酸发酵综合水平有所提高。     

Review of techniques to manufacture micro-hydrogel particles for the food industry and their applications

Microgels are ‘soft’ microscopic cross-linked polymeric particles that are being increasingly exploited in a variety of industries for rheology control, encapsulation and targeted delivery. They are valued because of the ability to tune their functionality to address specific applications in oil recovery, coatings, drug delivery, cosmetics, personal care and foods. Food microgels are typically biopolymer hydrogels in the form of microspheres, nanospheres (also called nanogels), spheroids and fibres. The utilisation of engineered microgels in foods has so far been limited, despite their great potential to address several needs in the food industry, including: satiety control, encapsulation of phytonutrients and prebiotics, texture control for healthier food formulations (e.g. reduced fat products), and targeting delivery to specific areas in the digestive tract. We review the scientific and patent literature on the utilisation and manufacturing methods for producing microgels with an emphasis on micro-hydrogels for food applications.     

12—A CRITIQUE OF SOME HYPOTHESES RELATING TO THE HETEROGENEITY OF THE HIGH-SULPHUR PROTEINS OF WOOL

Joubert and Burns prepared a large number of fractions from the high-sulphur proteins of wool and estimated their molecular weights and amino-acid compositions. Their data have been re-examined in order to look for statistically significant interrelations between amino acids and between the proportion of various amino acids and molecular weight. Statistical analysis of the data is also used to examine the credibility of some hypotheses concerning the mechanism of keratin biosynthesis and to provide further evidence for the existence of families of proteins within the high-sulphur fractions of wool.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of     

The Latest Data about Development of Chinese Printing Industry in 2013

In the 2014 China（Shanghai）International Printing Week,Director Wang Yanbin released the latest data about development of Chinese printing industry in 2013.According to statistics,in 2013,the total output value of Chinese printing industry exceeded 1trillion Yuan for the first time,reaching 1.03985 trillion Yuan.There were 105,000 printing enterprises in China,employees were 3.415 million.The total asset was 1.06247 trillion Yuan;     

The State Council Issued "Directory of Government Approved Investment Projects(2013 Edition)"

正On December 2nd,2013,the State Council issued the notification of"Directory of Government Approved Investment Projects(2013 Edition)"(hereafter referred to as"notification").It is pointed out in the"notification"that in order to further deepen reforms in investment systems and administrative examination and approval systems,simplify administrative procedures and delegate powers to lower levels,earnestly     

China textile machinery: taking off in progress

正Among the 1600 exhibitors who take apart in the ITMA ASIA+CITME2014 2/3 are Chinese manufactures.If the numerous figures failed to attract your attention,the increase of quality should draw your focus.To adopt the demand of developing textile machine market,domestic textile machinery enterprises now follow the slogan of"technology drives development"to enhance product competitiveness.Our domestic sellers will showcase product ranging from spinning,weaving,dyeing and printing,     

Top Ten News of China's Paper Industry 2013

On December 24th, 2013, the meeting on the selection of top 10 news of China＇s paper industry 2013 sponsored by 〈China Paper Newsletters〉 was held in Beijing. The yearly selection of the top l0 news, which began in 2000, has become a brand activity widely recognized in the industry thanks to the support from the authorities at all levels and public participation.     

National Development and Reform Commission Issued Announcement for Selection of Major Preliminary Research Projects for the "13~(th) Five-Year Plan"

In Apri Commi major p Plan＂ （h 2014, the National Development and Reform ssion issued an announcement for selection of reliminary research projects for the ＂13th Five-Year ereafter referred to as ＂The Announcement＂）     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the latest scientific     

Contamination of animal feed and food from animal origin with mineral oil hydrocarbons

Oils and fats used for the production of animal feed can become contaminated with mineral oil material originating from gas oils (C₁₈-C₃₅) or synthetic oils (poly alpha olefins, C₂₅ to beyond C₄₅). An important cause is assumed to be the discharge of waste oils, such as motor oil and hydraulic oils. Mineral oil material was analysed by on-line LC-GC-FID directly in the fat or in a raw extract from animal feed or foodstuffs. In Switzerland in summer/autumn 1999 concentrations in oils and fats for feed production were often found to be between 100 and 1000mg/kg. In the feeds, the average concentration was around 100mg/kg with values ranging up to a maximum of 1000mg/kg; few samples were free of contamination. In animal body fat, the average concentration determined in summer 1999 was 25mg/kg, with a maximum of 150mg/kg, although in samples from December 1999, contamination was substantially lower. In the fat phase of eggs, the average concentration was 30mg/kg, with a maximum of 80mg/kg. Paraffin oil is used for feed production, which may account for part of the contamination problem (e.g. eggs).