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1.
目的:建立一种虾中农药硫丹残留的快速检测方法.方法:采用酶联免疫吸附试验(ELISA)测定农药硫丹残留.提取溶剂为丙酮.该检测方法中用阴性样品作标准曲线.结果:该方法的检出限(IC15)约为1.6 μg/L,灵敏度(IC50)约为17μg/L.线性范围1.8~60μg/L.平均回收率67.56%~99.81%.结论:该检测方法符合<日本肯定列表>中规定的水产品中硫丹的检测要求,且样品的处理过程较为简单,适用于大量样品的快速检测.  相似文献   

2.
在多克隆抗体的基础上研制一种适用于检测样品中毒死蜱残留的间接竞争酶联免疫吸附分析(ELISA)试剂盒,并对试剂盒的灵敏度、特异性、精密度、准确度和有效期进行测定。结果表明,研制的试剂盒最低检测限为0.5μg/L,线性检测范围为1.8~1000μg/L,供试样品检测结果的批内、批间变异系数均低于8%,回收率均高于85%。试剂盒在4℃或-20℃条件下至少可保存6个月。  相似文献   

3.
本文主要研究了雷洛昔芬抗体的制备和酶联免疫吸附测定(enzyme-linked immuno sorbent assay,ELISA)方法的初步建立。实验中采用丁二酸酐对雷洛昔芬进行衍生,合成了雷洛昔芬半抗原。采用碳二亚胺法,将雷洛昔芬半抗原与牛血清白蛋白(BSA)和卵清蛋白(OVA)偶联合成免疫原和包被抗原,并通过紫外光谱进行鉴定,结果显示雷洛昔芬与载体蛋白偶联成功。免疫大白兔制备了雷洛昔芬抗体,抗体效价达1.28×10~5,半数抑制浓度(half inhibit concentration,IC50)15.4μg/L,与其他类似抗雌激素药物没有交叉反应,说明所制备的抗体特异性好。通过优化抗原抗体反应浓度初步建立了雷洛昔芬ELISA方法,结果显示最佳抗原浓度为300μg/L,最佳抗体工作浓度为1:1.0×10~5,标准曲线在0.4~102.4μg/L范围内线性关系好,R~2=0.9853,最低检测能力0.4μg/L。本研究为进一步开发雷洛昔芬快速检测试剂盒提供了技术参考。  相似文献   

4.
拟除虫菊酯类农药多残留的IC-ELISA检测方法研究   总被引:1,自引:0,他引:1  
为检测样品中拟除虫菊酯类农药多残留,以间苯氧基苯甲酸(PBA)为半抗原,用活性酯法将PBA与牛白蛋白(BSA)偶联,制备多克隆抗体,并建立间接竞争酶联免疫吸附测定法。用方阵滴定确定包被抗原的最适工作浓度为5.0μg/mL,抗体最佳浓度为10.0μg/mL,该方法的最低检测限为0.301μg/mL,在0.1~200μg/mL工作浓度范围内,标准曲线表现良好的线性关系。  相似文献   

5.
建立气相色谱批量测定水产品中7种多氯联苯残留的分析方法。样品前处理采用超声波提取,经浓硫酸磺化后引入分散固相技术进行净化,考察不同条件下多氯联苯的提取分离效果并考虑水产品基质干扰的因素,利用气相色谱-电子捕获检测器测定7种多氯联苯含量,采用内标校正。结果表明:7种多氯联苯在0.005~0.2μg/m L质量浓度范围内具有良好的线性关系,相关系数0.999 86~0.999 97;样品中添加量为2~100μg/kg时,回收率为80.4%~118.0%,相对标准偏差为1.9%~7.3%,检出限为(RSN=3)0.4~0.8μg/kg,定量限(RSN=10)1.3~2.7μg/kg。该方法操作简单、方便快速、重复性好,适用于批量监测水产品中多氯联苯残留的分析。  相似文献   

6.
采用过碘酸钠法将抗环丙沙星单克隆抗体(MAb-CIP)与辣根过氧化物酶(HRP)偶联制备酶标抗体MAbCIP-HRP,建立了检测水产品中6种氟喹诺酮类药物残留的直接竞争酶联免疫吸附分析方法(dcELISA),考察了包被原浓度、竞争反应时间和有机溶剂等因素对方法灵敏度的影响。结果表明:在优化的反应条件下,所建立的dcELISA针对环丙沙星、恩诺沙星、诺氟沙星、培氟沙星、沙拉沙星和双氟沙星6种氟喹诺酮类药物的检测限(LOD)均不超过0.5ng/mL,线性范围(IC20~IC80)在1.0~12.1ng/mL之间;对虾、鳗鲡和鲫鱼三种水产样品中添加5.0、10.0和20μg/kg时,加标回收率为70.4%~104.1%,相对标准偏差为5.0%~14.7%;本方法可用于水产品中氟喹诺酮类(FQs)药物多残留的快速测定。  相似文献   

7.
采用酶标抗原建立了高效、高灵敏的呋喃唑酮代谢物直接竞争的ELISA检测方法。以呋喃唑酮单克隆抗体包被作为固相抗体,HRP标记的抗原与标准品(或样品)中呋喃唑酮的代谢物衍生物竞争结合抗体,建立了直接竞争酶联免疫检测体系。以3-氨基-2-恶唑烷酮的衍生物(CPAOZ)为标准品建立标准曲线,得到方法的IC50为0.08μg/L,灵敏度为0.015μg/L,线性范围0.025~0.5μg/L;检测样品的平均回收率为82.0%~121.0%,与其他结构类似物基本无交叉反应。建立呋喃唑酮酶标抗原直接竞争酶联免疫检测方法,灵敏度高、特异性强、操作简单,可以满足畜禽水产实际样品的检测需要。  相似文献   

8.
测定三种乳蛋白抗原性间接竞争ELISA法的建立   总被引:1,自引:0,他引:1  
为检测牛乳中主要致敏蛋白的抗原性,以商业兔抗αs-酪蛋白、兔抗β-乳球蛋白、兔抗牛血清白蛋白(BSA)多克隆抗体为一抗,标记HRP的羊抗兔Ig G为二抗,TMB为底物,对牛乳中主要致敏蛋白:αs-酪蛋白、β-乳球蛋白、BSA建立间接竞争ELISA(ic ELISA)。结果表明,αs-酪蛋白、β-乳球蛋白及BSA抗原包被质量浓度分别为5,2和2μg/m L。一抗最佳稀释倍数分别为1∶800,1∶400和1∶300。建立的三种蛋白的ic ELISA的线性检测范围分别为15.625~1 000 ng/m L、31.25~1 000 ng/m L和62.5~2 000 ng/m L。三种乳蛋白包被条件均为4℃,12 h,酶标二抗的最佳稀释倍数为1∶1 200,以1%的明胶作为封闭液。三种乳蛋白ic ELISA的3个浓度添加试验的批内及批间最大变异率分别为5.16%,7.46%,6.6%和6.78%,6.81%,6.06%,三种蛋白建立的ic ELISA回收率在94.34%~109.92%。表明所建立的方法重复性好,灵敏度高,可用于牛乳中此三种蛋白致敏性的测定。  相似文献   

9.
目的建立猪肉、鸡蛋、牛奶、猪脂肪等动物源性食品中同时检测17种农药残留的在线凝胶渗透色谱-气相色谱-质谱联用法(GPC-GC-MS)。方法样品用正己烷饱和乙腈溶液均质提取,加入氯化钠盐析,离心分层,取部分乙腈层经固相萃取(NH2)柱净化,利用在线GPC-GC-MS测定。结果在0.85~30μg/L范围内相关系数均在0.95以上,回收率在61.2%~126%之间。17种农药残留最低检出限范围(以信噪比S/N≥3计)为0.25~3.00μg/L,定量限范围(以信噪比S/N≥10计)为0.85~10.0μg/L。结论该法有效、耐用、快速、灵敏,符合法规残留限量检测要求。  相似文献   

10.
目的 建立了水产品中13种镇静剂残留的同位素稀释-液相色谱-串联质谱(HPLC-MS/MS)的测定方法。方法 样品采用乙腈-1%氨水溶液提取,提取液经固相萃取柱(Oasis PRIME HLB柱)净化、浓缩后用流动相定容。以1%甲酸水溶液和乙腈为流动相,采用Agilent InfiniityLab Poroshell 120 SB-C18色谱柱(2.1×100mm,2.7μm)分离后,在多反应监测(MRM)模式下测定,内标法定量。结果 13种镇静剂在0.2-50μg/L浓度范围内线性关系良好(r>0.99),方法的检出限(LOD)和定量下限分为0.1~0.2μg/kg和0.3~0.5μg/kg。在3个浓度加标水平下的平均回收率为84.0~116.6%,相对标准偏差(RSD)为2.1~9.9%。结论 该方法前处理简单、结果准确,适用于水产品种13种镇静剂残留的快速检测。  相似文献   

11.
Since grapevine ( Vitis spp .) rootstock material is being traded increasingly as disbudded woody material a lack of distinctive morphological features on such material necessitates an alternative and reliable means of identification. Methods described here were developed for rapid and efficient extraction of DNA from woody samples rich in phenolic compounds and polysaccharides, and for subsequent identification of varieties by RAPD PCR. Using these methods, and with the application of only one selected RAPD primer, we were able to differentiate sixteen rootstock varieties, including the seven varieties most commonly used in Germany. Problems commonly encountered with reproducibility of RAPD patterns were avoided by choosing primers with a dinucleotide sequence and a high G/C content that allowed a rather high annealing temperature of 45°C. Methods described here should also be useful for other horticultural crops, especially those with woody tissues rich in phenolic compounds and polysaccharides.  相似文献   

12.
An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.  相似文献   

13.
The characterization of the aromatic profile of several apricot cultivars with molecular tracers in order to obtain objective data concerning the aromatic quality of this fruit was undertaken using headspace–solid phase microextraction (HS–SPME). Six apricot cultivars were selected according to their organoleptic characteristics: Iranien, Orangered, Goldrich, Hargrand, Rouge du Roussillon and A4025. The aromatic intensity of these varieties measured by HS–SPME–Olfactometry were defined and classified according to the presence and the intensity of grassy, fruity and apricot like notes. In the six varieties, 23 common volatile compounds were identified by HS–SPME–GC–MS. Finally, 10 compounds, ethyl acetate, hexyl acetate, limonene, β-cyclocitral, γ-decalactone, 6-methyl-5-hepten-2-one, linalool, β-ionone, menthone and (E)-hexen-2-al were recognized by HS–SPME–GC–O as responsible of the aromatic notes involved in apricot aroma and considered as molecular tracers of apricot aromatic quality which could be utilized to discriminate apricot varieties.  相似文献   

14.
The advent of the functional barrier concept in food packaging has brought with it a requirement for fast tests of permeation through potential barrier materials. In such tests it would be convenient for both foodstuffs and materials below the functional barrier (sub-barrier materials) to be represented by standard simulants. By means of inverse gas chromatography, liquid paraffin spiked with appropriate permeants was considered as a potential simulant of sub-barrier materials based on polypropylene (PP) or similar polyolefins. Experiments were performed to characterize the kinetics of the permeation of low molecular weight model permeants (octene, toluene and isopropanol) from liquid paraffin, through a surrogate potential functional barrier (25 μm-thick oriented PP) into the food simulants olive oil and 3% (w/v) acetic acid. These permeation results were interpreted in terms of three permeation kinetic models regarding the solubility of a particular model permeant in the post-barrier medium (i.e. the food simulant). The results obtained justify the development and evaluation of liquid sub-barrier simulants that would allow flexible yet rigorous testing of new laminated multilayer packaging materials.  相似文献   

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16.
Capillary electrophoresis (CE) and polarized light microscopy (PLM) were utilized in the detection of the adulteration of locust bean gum with guar gum. For CE analyses, standards of locust bean and guar gums were extracted with 30% CH3CN, removing the residual proteins from the gum matrix. A 8.75 mM NaH2PO4-20.6 mM Na2B4O7 buffer, pH 9, was used to separate these proteins and to identify marker proteins that were present in the guar gum. These markers did not co-migrate with components in the extracts of mechanically processed locust bean gum, and are used as indicators of adulteration. Using PLM with toluidine blue and iodine staining techniques, unadulterated locust bean gum samples were distinguished from mixed samples through the differential staining of components in locust bean versus guar and tara gums. These experiments in the use of CE and PLM provide orthogonal and complementary methods for the verification of 'true' positives and the elimination of 'false' positives.  相似文献   

17.
An investigation is described in which various polyfunctional compounds were applied to wool in attempts to stabilize the temporary improvements in wrinkle-recovery brought about by ‘annealing’. Several reactive systems involving formaldehyde were found to produce the desired permanently improved wrinkle-recovery.  相似文献   

18.
A 9% whey protein (WP) isolate solution at pH 7.0 was heat-denatured at 80°C for 30 min. Size-exclusion HPLC showed that native WP formed soluble aggregates after heat-treatment. Additions of CaCl2 (10–40 mM), NaCl (50–400 mM) or glucono-delta-lactone (GDL, 0.4–2.0%, w/v) or hydrolysis by a protease from Bacillus licheniformis caused gelation of the denatured solution at 45°C. Textural parameters, hardness, adhesiveness, and cohesiveness of the gels so formed changed markedly with concentration of added salts or pH by added GDL. Maximum gel hardness occurred at 200 mM NaCl or pH 4.7. Increasing CaCl2 concentration continuously increased gel hardness. Generally, GDL-induced gels were harder than salt-induced gels, and much harder than the protease-induced gel.  相似文献   

19.
BADGE.2HCl and BFDGE.2HCl were determined in 28 samples of ready-to-drink canned coffee and 18 samples of canned vegetables (10 corn, 5 tomatoes and 3 others), all from the Japanese market. HPLC was used as the principal analytical method and GCMS for confirmation of relevant LC fractions. BADGE.2HCl was found to be present in one canned coffee and five samples of corn, BFDGE.2HCl in four samples of canned tomatoes and in one canned corn. No sample was found which exceeded the 1mg/kg limit of the EU for the BADGE chlorohydrins. However the highest concentration was found for the sum of BFDGE.2HCl and BFDGE.HCl.H2O at a level of 1.5mg/kg. A Beilstein test confirmed that all cans containing foods contaminated with BADGE.2HCl or BFDGE.2HCl had at lest one part coated with a PVC organosol.  相似文献   

20.
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