脂肪球在搅打乳状液中的部分聚结及其作用

综述了脂肪球在搅打乳状液中的部分聚结现象和搅打条件、脂肪种类、脂肪球吸附膜对部分聚结的影响。以冰淇淋和搅打奶油为例 ,说明脂肪球的部分聚结对于搅打乳状液最终形成充气的泡沫结构起关键作用 ,控制脂肪球的部分聚结程度对于改善搅打乳制品的质量、性能具有重要意义。联系控制脂肪球的部分聚结在冰淇淋生产中的实际应用 ,介绍了近来国际上有关脂肪球部分聚结的研究进展。     

油脂熔点对植脂鲜奶油质量性能的影响及其机理的研究

利用质构分析仪分析泡沫硬度和内部质构,油溶性色素法(油红-O)测定脂肪球部分聚结率,研究油脂种类对植脂鲜奶油质量性能的影响.结果表明,不同种类油脂制备的植脂鲜奶油的质量性能有较大差异,油脂种类对质量性能的影响与其熔点有关.随油脂熔点不同,在搅打过程中发生脂肪球部分聚结的程度也不同,从而影响最终的泡沫结构.搅打5 min后,熔点为47～53℃的部分氢化植物油HP-51的部分聚结率大于70%,天然奶油的部分聚结率小于10%,大豆油和极度氢化油不发生部分聚结,均不能形成稳定的泡沫结构.熔点为36～41℃的部分氢化植物油制备的植脂鲜奶油,部分聚结率为65%,膨胀率为4.0,获得稳定的泡沫结构.     

瓜尔豆胶对搅打稀奶油的搅打性能的影响

研究了不同浓度的瓜尔豆胶对搅打稀奶油乳状液的表观黏度、脂肪球粒度、脂肪球界面蛋白浓度、脂肪球部分聚结率、泡沫硬度和搅打起泡率的影响。结果表明，瓜尔豆胶对搅打稀奶油乳状液的表观黏度影响非常显著；瓜尔豆胶浓度过高或过低，都会使得解冻后的乳状液粒径变大；瓜尔豆胶的质量分数越高，脂肪球部分聚结速度越快，泡沫硬度也越大；搅打起泡率随着瓜尔豆胶质量分数增大而降低。     

植脂奶油泡沫结构形成的研究

分别测定植脂奶油乳状液在剪切、充气和搅打作用下脂肪球部分聚结率、电导率、膨胀率的变化,研究了植脂奶油形成泡沫结构的作用机理.结果表明,剪切或充气作用都不能形成稳定的泡沫结构;只有在搅打条件下才能获得60%以上的脂肪球部分聚结率,使植脂奶油由一个黏稠的液体转变为稳定的泡沫结构.     

乳化剂用量对搅打稀奶油搅打性能和品质的影响机理研究

研究了乳化剂用量对搅打稀奶油的乳浊液粒度分布、脂肪球部分聚结、液相蛋白浓度、感官品质和泡沫稳定性的影响。研究结果表明:乳浊液冷却及解冻后脂肪球粒径随乳化剂用量增加而减小,随着乳化剂用量增加,脂肪部分聚结速度和液相蛋白质浓度增加速度加快,搅打起泡率和感官品质以乳化剂用量为0.60%时最好,搅打稀奶油稳定时间随乳化剂用量增加呈先增加后下降趋势,当乳化剂用量为0.60%时,稳定时间达到最大2.4h。综合考虑,当乳化剂HLB值为7,乳化剂用量为0.60%时,搅打稀奶油搅打性能和品质最佳。     

油脂种类对植脂鲜奶油搅打性能的影响

通过研究不同种类的油脂在植脂鲜奶油搅打过程中脂肪球的部分附聚、搅打时间、搅打起泡率和泡沫稳定性之间的关系。结果表明：不同种类的油脂对搅打性能的影响与其熔点有着密切的关系，其实质是油脂在常温下的固体脂肪指数决定了脂肪球能否发生部分聚结与聚结的程度，熔点为35～43℃的部分氢化植物油的聚结率接近60％，可以达到最佳的部分聚结率，能够形成稳定的泡沫结构；熔点为47～53℃的部分氢化植物油的聚结率超过70％，容易产生过度的部分聚结，形成结构粗糙的泡沫；天然奶油的部分聚结率低于10％；大豆油或者极度氢化油不能发生脂肪球的部分聚结，不能形成稳定的泡沫结构。     

蛋白质用量对黄油基淡奶油搅打前乳液性质及搅打性能的影响

以黄油为油相制备淡奶油,研究蛋白质用量(1.0%～3.0%)对黄油基淡奶油搅打前乳液性质及搅打性能的影响。研究表明:蛋白质用量为1.0%～2.5%时,随蛋白质用量的增加,搅打前乳液的界面蛋白浓度明显增加,脂肪部分聚结率降低,脂肪球粒径减小,粒度分布曲线变窄,表观黏度升高,乳液的稳定性提高;蛋白质用量由2.5%增加至3.0%时,脂肪部分聚结率增加,脂肪球粒径增大,乳液的稳定性降低。乳液在200 s的搅打过程中,随搅打时间的延长,脂肪部分聚结率不断增加,平均粒径呈现先减小后增大的趋势,搅打起泡率则先升高后降低。蛋白质用量为1.0%～1.5%时,在搅打过程中脂肪部分聚结率、平均粒径、搅打起泡率均快速增加,易出现搅打过度现象,且泡沫稳定性差。蛋白质用量为2.0%～2.5%时,脂肪部分聚结率、平均粒径、搅打起泡率则缓慢增加,而泡沫稳定性较好。蛋白质用量增加至3.0%时,尽管泡沫稳定性较好,但搅打200 s后的脂肪部分聚结率仅有26.45%。因此,将黄油基淡奶油的蛋白质用量控制在2.0%～2.5%范围内较适宜。     

油脂用量对搅打稀奶油的搅打性能和品质的影响

研究了油脂用量对搅打稀奶油的粒度分布、脂肪部分聚结、液相蛋白质浓度、搅打起泡率、质构特性、感官品质和稳定时间的影响。研究表明:随着油脂用量增加,冷却后乳浊液脂肪球粒径增大;搅打过程中脂肪部分聚结速度和脂肪球粒径d4,3均随油脂用量增加而增大,且脂肪部分聚结率与脂肪球粒径d4,3有很好的相关性;液相蛋白质浓度和搅打起泡率降低;搅打稀奶油的质构特性值增加;稳定时间呈先增后减趋势,当油脂用量为23%时,搅打稀奶油的稳定时间最长达到2.7h;搅打稀奶油的感官品质以油脂用量为20%最好,综合考虑,油脂最佳用量范围是20%-23%。     

搅打稀奶油品质及其影响因素的研究进展

搅打稀奶油是一类可搅打起泡的水包油乳液。作为重要的食品工业原料，搅打稀奶油可作为呈味物质赋予食品良好的感官特性，应用前景广阔。本文首先介绍搅打稀奶油贮藏期内常见的失稳作用，包括乳析、聚集、聚结、部分聚结及奥氏熟化，并阐述界面特性及脂肪球间的交互作如何影响搅打稀奶油的稳定性；其次介绍搅打稀奶油的搅打过程，阐述结晶特性、界面特性和液相蛋白特性如何影响搅打特性。本文可为搅打稀奶油产品的工业化生产提供一定的理论指导。     

黄油-代可可脂基奶油生产工艺优化及其晶型形成分析

在分析油脂用量对黄油-代可可脂基奶油品质影响的基础上,通过正交试验研究油脂用量、黄油-代可可脂质量比和均质压力对奶油搅打性能、质构特性、脂肪球部分聚结率、结晶特性等的影响。结果表明,在油脂用量30%、黄油-代可可脂质量比2∶3、均质压力60?MPa的条件下,能保证脂肪部分聚结的发生,并保持搅打稀奶油较好的感官特性、起泡性和结晶特性。60?MPa的均质压力保证了体系的稳定性,使大量游离脂肪球产生从而促进脂肪部分聚结。晶型形成分析结果表明,在优化条件下,原料乳浊液X-射线衍射峰峰形由较宽、较尖锐变为峰形适中均匀,适宜搅打稀奶油的β’晶型晶体明显增多,搅打稀奶油乳浊液在低温条件下发生脂肪结晶,表现为脂肪晶体尺寸增大。     

Microstructure of Feta Cheese Made Using Different Cultures as Determined by Confocal Scanning Laser Microscopy

ABSTRACT: The objective of this work was to observe the microstructure of feta cheese using confocal scanning laser microscopy. Low fat cheese and cheese containing capsule-producing cultures were made. The protein network was observed using the reflectance mode of the confocal microscope. Fat was stained by Nile red dye diluted with whey. More even distribution of fat with a larger number of smaller globules was observed in cheese made with noncapsule-forming culture compared to that made with the capsule-forming culture. Nonfat cheese had compact structure when made with noncapsule-forming culture which contrasted with the open structure observed in cheese made with the capsule-forming culture     

Decolorization of azo dye by the white-rot basidiomycete Phanerochaete sordida and by its manganese peroxidase

We investigated the decolorization of an azo-reactive dye, Reactive Red 120, by a white-rot basidiomycete, Phanerochaete sordida strain YK-624. In liquid culture of P. sordida in a medium containing 3% malt extract and 200 mg/l of the dye, the dye was 90.6% decolorized after 7 d. Manganese peroxidase (MnP) activity was detected during the decolorization process. The dye could be decolorized by purified MnP of P. sordida in the presence of Mn(II) and Tween 80. The involvement of lipid peroxidation during decolorization with MnP was considered. With shaking, the dye could be decolorized without the addition of hydrogen peroxide. The decolorization did not occur under anaerobic conditions, suggesting that dye decolorization by MnP is influenced by dissolved oxygen. Since catalase did not inhibit the decolorization with MnP, we inferred that the MnP catalytic cycle would be promoted by hydroperoxides formed from the decomposition of malonate or from lipid peroxidation.     

Effects of dietary soybean oil on lipid and protein oxidation in pork patties during chill storage

The effect of dietary soybean oil on lipid and protein oxidation in low and high fat pork patties made from quadriceps femoris during chill storage in a high oxygen atmosphere packaging (80% O(2)/20% CO(2)) in the dark for 7 days was investigated. Pigs were fed either a standard diet or a diet added 2% soybean oil. After slaughter high fat pork patties were prepared for both feeding regimes by addition of back fat from pigs fed the same diet whereas low fat pork patties were prepared without addition of back fat. The 2% soybean diet increased the amount of unsaturated fat in the pork. Secondary lipid oxidation products determined as thiobarbituric acid reactive substances (TBARS) were found to increase in the pork patties with increased unsaturated fat. Increased unsaturated fat in the pork patties had no effect on protein oxidation determined as free protein thiol content and protein carbonyl content. A small, but significant increase in protein oxidation was found in the high fat pork patties independent on dietary fat. In conclusion, protein oxidation is unaffected by dietary fat in pork patties during chill storage for periods normally used in retail trade, and lipid and protein oxidation are not coupled under these conditions.     

LIPID AND CHOLESTEROL OXIDATION,COLOR CHANGES,AND VOLATILE PRODUCTION IN IRRADIATED RAW PORK BATTERS WITH DIFFERENT FAT CONTENT1

An emulsion-type product was prepared to determine the effect of irradiation on lipid and cholesterol oxidation, color change, and volatile production in raw pork with different fat contents. Lipid oxidation increased with an increase in fat content or irradiation dose. Irradiated batters had higher cholesterol oxides than nonirradiated, and the major cholesterol oxides formed in irradiated pork batters were 7α- and 7β-hydroxycholesterol. Hunter color a- and b-values of raw pork batters were decreased by irradiation regardless of fat content. Irradiation increased the amount ofvolatiles significantly. Although lipid oxidation of high fat products (10 and 15% fat) was higher than that of low fat products (4%), high fat products did not always produce greater amount of volatiles. In summary, irradiation increased lipid and cholesterol oxidation, volatiles production and had detrimental effects on the color of raw pork batters under aerobic condition.     

Composition of Cooked Pork Chops: Effect of Removing Subcutaneous Fat Before Cooking

Conventionally trimmed (0.6 cm) and completely trimmed (0 cm) prok chops were used to evaluate the effect of removing subcutaneous fat on lipid retention during broiling of pork. Composition of the lean portion of cooked chops was not affected by removal of external fat before cooking. Conventional chops contained 4.4% either extractable lipid and completely trimed chops contained 4.3%. Lipid retention was also the same at 94–99%. Recovery of moisture, protein, and lipid was 98–99%, using ether extraction or chloroform:methanol extraction. Therefore, the concept of lipid migration during cooking was not a tenable explanation for reports of lipid retention exceeding 100% during broiling of pork chops typical of those available to consumers.     

Injecting Fats into Breast Meat of Turkey Carcasses Differing in Finish and Retention After Cooking

Retention of butter and coconut oil injected into the major breast muscles of uncooked turkey was examined. Fats were injected to 3% of thawed weight using carcasses differing in skin depots covering the breast of each sex. Cooking time was not influenced by fat injection nor was the yield of breast meat. Injecting fats led to a reduction in muscle moisture while petroleum ether extract increased. Fatty acid analysis of the extract indicated that the lipid increases were representative of the fat injected, and the extent of retention was equivalent with each fat source. Skin depots over the breast and sex of the turkey had little effect on muscle lipid and fat retention.     

A Quantitative and Morphological Study of Bovine Longissimus Fat Cells

SUMMARY— A quantitative and morphological study was made of bovine fat cells from three marbling groups of longissimus muscle. Tracings on acetate paper were made of the visual marbling depots and the area measured at three positions with an ocular grid for both size and distribution of fat cells. Comparisons were also made of subcutaneous, intermuscular and intramuscular fat cell size. Intrafiber lipid accumulation was observed and the average number of red fibers per bundle determined. These results indicate that traceable intramuscular fat is not a good measure of total intramuscular fat. There did not appear to be a consistent medial, central and lateral marbling pattern among the three marbling groups; however, significant differences were apparent within groups. Fat cell size increased with increases in cell mass, marbling and total chemical fat of the muscle.
Fat cells accumulated and grew in close proximity to portions of the circulatory system. Lipid deposits adjacent to the muscle contained larger fat cells than was evident in the extrafascicular spaces within the muscle. Intrafiber lipid was readily apparent in approximately 35% of the muscle fibers and probably represented either mitochondria or triglyceride. The interrelationship of subjective marbling scores, chemically determined fat, fat cell size, fat cell distribution and intrafiber lipid characteristics are complex and require complete investigation before the association of muscle fat and meat quality can be resolved.     

猪腿肉脂肪酸组成及脂肪氧化的研究

本文研究了不同性别英系大白腿肉中肌间脂肪和皮下脂肪含量,并对其脂肪酸组成进行了分析,同时研究了腿肉在(4±0.5)℃避光贮存条件下脂肪氧化程度的变化,结果表明,不同性别猪腿肉肌间和皮下脂肪酸组成及含量均没有显著性差异(p>0.05);在(4±0.5)℃避光贮存期间(0～5d),肌间脂肪氧化程度显著高于皮下脂肪氧化程度(p<0.05),尤其到第5d时,肌间脂肪氧化指标-TBA值接近0.5mg/kg,而皮下脂肪TBA值低于0.2mg/kg。一般来讲,当生肉的TBA值超过0.5mg/kg时,人就能感觉到有氧化异味。因此,控制肌间脂肪氧化保证冷却肉良好品质的关键。     

红茶菌对饮食诱导肥胖小鼠体重控制的研究

本实验以高脂饲料喂食的C57BL/6J 小鼠为动物模型,进行为期11 周的实验,以小鼠体重增加、体内脂肪含量、血清脂质水平、小鼠脏器系数、肝组织形态、脂肪组织形态为检测指标,研究茶叶浸出液、A 4(Gluconacetobacter sp. A4)发酵液、红茶菌原液发酵液对小鼠体重控制的影响。结果表明,茶叶浸出液、A4 发酵液、红茶菌原液发酵液,均能够减缓小鼠体重增长的进程,减少小鼠体内脂肪含量,并能够减少脂肪在肝脏中的蓄积以及减缓脂肪细胞增大的进程。其中,以A4 发酵液的效果最为明显,表现出对饮食诱导小鼠的很好的体重控制的作用。     

Peripartal metabolism and production of holstein cows fed diets supplemented with fat during the dry period

Previous research from our laboratory demonstrated that cows fed supplemental fat throughout the dry period in an attempt to increase body condition score (BCS) had little hepatic lipid accumulation at d 1 postpartum compared with cows fed an isocaloric high-grain diet or a lower energy control diet. However, results were confounded by lower dry matter intake and loss of BCS by cows fed the fat-supplemented diet. Here, cows were fed a control diet (C) moderately high in nonfiber carbohydrates (NFC) or an isocaloric fat-supplemented, low NFC (F) diet to reassess the effects of supplemental fat throughout the dry period on peripartal lipid accumulation in liver. A more energy-dense, high-NFC diet supplemented with fat (CF) was also fed to test the efficacy of supplemental fat in a diet with similar carbohydrate composition but higher energy density. Intakes of dry matter and net energy for lactation were similar among treatments throughout the experiment, although diet x day interactions during the last 21 d before parturition indicated that cows fed CF decreased intakes more slowly. Cows gained similar amounts of BCS and body weight among diets prepartum, but cows fed C tended to lose more BCS and body weight around parturition. Milk production and milk components did not differ among treatments. Prepartum concentrations of glucose, insulin, total protein, nonesterified fatty acids, and mu-hydroxybutyrate in plasma were similar among treatments. Supplemental fat increased prepartum concentrations of urea and cholesterol in plasma. Postpartum concentrations of metabolites and insulin in plasma were similar among treatments. Concentrations of total lipid and triglyceride in liver increased at parturition, whereas hepatic glycogen concentration decreased, but concentrations were not different among treatments. Supplemental fat fed prepartum did not affect peripartal lipid accumulation in liver tissue and did not benefit postpartum milk production.