桦褐孔菌乙醇粗提物对朊病毒复制的抑制作用

优化桦褐孔菌乙醇粗提物提取工艺并初步探究其对朊病毒复制的影响。采用醇提方法,利用正交试验方法,确定最佳提取工艺。使用CCK-8试剂盒对桦褐孔菌乙醇粗提物处理细胞的安全性进行评价,确定其安全质量浓度;根据安全性评价结果,使用蛋白免疫印记（Western blot）方法检测桦褐孔菌乙醇粗提物对朊病毒复制的影响;通过测定过氧化氢及抗氧化因子等对桦褐孔菌乙醇粗提物的抗氧化活性进行研究。桦褐孔菌乙醇粗提物的最佳提取工艺为：料液比（桦褐孔菌生粉:乙醇）1:10（g/mL）,乙醇体积分数75%,提取时间2 h,提取温度80 ℃,在此条件下,得率为6.32%。桦褐孔菌乙醇粗提物的最大安全质量浓度为6.25×10-4 mg/mL;在此浓度下,处理细胞24 h后,桦褐孔菌乙醇粗提物对朊病毒的复制有抑制作用（p<0.01）,可减少H2O2的含量,增加SOD和抗氧化因子GCLM、GCLC的含量,且具有统计学意义。本试验表明桦褐孔菌乙醇粗提物对朊病毒的复制具有抑制作用,且这种作用可能与其抗氧化活性有关。     

桦褐孔菌多糖磷酸化修饰工艺研究

桦褐孔菌多糖是桦褐孔菌中主要的生物活性成分,在抗氧化、提高免疫力等方面具有重要作用和广泛的药用价值。该文以桦褐孔菌为研究对象,以桦褐孔菌多糖溶液中磷酸根含量为指标,通过单因素试验及响应面法对桦褐孔菌多糖磷酸化修饰工艺条件进行优化,以提高其多糖的生物活性。结果表明,桦褐孔菌多糖磷酸化修饰最佳工艺条件：三聚磷酸钠与三偏磷酸钠质量比为6∶1、反应温度为75℃,反应时间为4 h,pH值为8.6,修饰后的桦褐孔菌多糖溶液中磷酸根含量为9.58%。该结果为桦褐孔菌多糖磷酸化衍生物的开发提供参考依据。     

发酵时间对桦褐孔菌主要活性成分的影响

该文主要研究桦褐孔菌在液体发酵过程中主要活性成分多糖、三萜甾醇的累积与代谢变化。采用液体发酵法培养桦褐孔菌获得不同发酵时间的发酵产物,分别采用苯酚硫酸法及HPTLC分析法检测不同培养时间的菌丝体中多糖、三萜和甾醇的含量,DPPH与硫酸香草醛显色法检测抗氧化活性。结果显示,随发酵时间的延长,桦褐孔菌菌丝体生物量越来越高,经HPTLC法分析可见发酵后的菌丝体与桦褐孔菌子实体中的三萜和甾醇成分相似。且多糖、三萜甾醇的含量均呈现先增高后降低的趋势,而通过显色反应,发现菌丝体中含有子实体未发现的抗氧化三萜成分。通过研究确定了桦褐孔菌液体发酵过程中主要活性成分变化,确立最佳发酵时间,为桦褐孔菌的工业化生产提供参考。     

超声波辅助提取桦褐孔菌子实体中多糖的工艺优化

研究了优化超声提取桦褐孔菌中多糖的工艺.以蒸馏水为提取剂,用超声波辅助提取桦褐孔菌多糖类物质,以不同处理条件、超声功率、超声次数、超声处理时间为试验因素,以多糖的提取率为考察指标进行单因素试验确定最佳提取工艺.超声辅助技术提取桦褐孔菌子实体中多糖类的最佳工艺条件为:超声功率400W,超声20次,超声10min,在此条件下,多糖得率最高,为11.62％.     

桦褐孔菌深层发酵培养条件的优化研究

以多糖和生物量的得率为指标,研究了不同碳源、氮源、无机盐、温度、初始pH值、接种量等不同因素对桦褐孔菌深层发酵的影响,运用了单因素试验的方法探索了其最佳的液体培养条件。确定了促进桦褐孔菌菌丝体发酵生产多糖的培养基组成为1.8%蔗糖,0.2%麸皮,0.05%MgSO.47H2O和0.1%KH2PO4。适宜发酵多糖的条件为:发酵温度25℃,培养基初始pH值为8.5,接种量为10%。     

大孔吸附树脂分离纯化桦褐孔菌三萜工艺研究

为研究大孔吸附树脂纯化桦褐孔菌三萜的工艺,筛选了适宜的大孔吸附树脂,并以吸附率和解析率为指标,利用最适的树脂进行对桦褐孔菌的静态吸附和解析的条件优化,研究了上样液体积、吸附时间、样液pH值、解析时间、解析液种类和解析液浓度的影响,确定纯化工艺参数。结果表明:在供试的5种大孔吸附树脂中,AB-8型大孔吸附树脂适合分离桦褐孔菌三萜。最佳分离条件为:样液在自然pH值条件下,按10mL/g树脂进行吸附12h后利用蒸馏水除去未吸附的样液后选择30%乙醇及70%乙醇进行依次解析12h,此时三萜的吸附率和解析率分别可达93%和80%以上。表明AB-8型大孔吸附树脂纯化桦褐孔菌三萜的效果较佳。     

醇水交换法提取桦褐孔菌生物活性物质的工艺研究

目的:利用超声热学及空化技术,研究醇-水交换提取桦褐孔菌中的三萜类和多糖生物活性物质的最佳提取工艺。方法:桦褐孔菌粉碎,过30目筛,超声辅助醇-水交换提取其生物活性物质。结果:醇提三萜类物质最佳工艺为:温度30℃,时间50min,提取3次,液料比25∶1,得率为1.30%;水提多糖最佳工艺为:温度50℃,时间40min,提取3次,液料比35∶1,得率为6.96%。结论:超声辅助醇-水交换提取桦褐孔菌中的生物活性物质的最佳提取工艺稳定、合理可行,能有效提高桦褐孔菌的综合利用。     

超声波辅助提取桦褐孔菌子实体中多糖和三萜

研究了优化超声辅助提取桦褐孔菌中多糖和三萜类物质的工艺。以蒸馏水和异丙醇为提取剂,用超声波辅助提取桦褐孔菌多糖和三萜类物质,以不同处理条件、超声功率、超声次数、超声处理时间为试验因素,以多糖和三萜的提取率为考察指标进行单因素实验确定最佳提取工艺。超声辅助技术提取桦褐孔菌子实体中多糖类和三萜类物质的最佳工艺条件为:超声功率400 W、超声20次、超声10 min,在此条件下多糖得率最高,为11.62%,总三萜类物质得率在2%,使得多糖和三萜类物质二者共提取得率达到13.57%。     

基于响应面法的闪式提取桦褐孔菌多糖工艺优化

利用响应面法优化桦褐孔菌多糖的闪式提取工艺。在单因素实验的基础上,以桦褐孔菌多糖的提取率为响应值,以料液比、提取时间、提取电压为主要考察因素,采用响应面法优化闪式提取桦褐孔菌多糖的最佳工艺参数。结果表明,经响应面优化得到料液比为1∶23,提取时间为86 s,提取电压为150 V时,桦褐孔菌多糖的提取率最高,可达到8.52%。结论:闪式提取法适用于桦褐孔菌多糖的提取,该工艺具有方便、快速和高效的优点。且经响应面法优化所得的最佳提取条件准确可靠,具有一定的实用价值。     

桦褐孔菌多糖研究现状与展望

桦褐孔菌（Inonotus obliquus）富含多糖、萜类、多酚等多种活性成分,具有抗癌、抗衰老、降血糖等多种药理作用。多糖作为桦褐孔菌最主要的活性物质,近年来成为食品和医药领域的研究热点。该文从桦褐孔菌多糖诱导发酵、提取工艺、分离纯化及作用功效等方面,综述近年来桦褐孔菌多糖研究的概况,并对存在的问题及其应用前景进行展望,旨在为桦褐孔菌多糖深入研究和开发应用提供参考。     

Review of techniques to manufacture micro-hydrogel particles for the food industry and their applications

Microgels are ‘soft’ microscopic cross-linked polymeric particles that are being increasingly exploited in a variety of industries for rheology control, encapsulation and targeted delivery. They are valued because of the ability to tune their functionality to address specific applications in oil recovery, coatings, drug delivery, cosmetics, personal care and foods. Food microgels are typically biopolymer hydrogels in the form of microspheres, nanospheres (also called nanogels), spheroids and fibres. The utilisation of engineered microgels in foods has so far been limited, despite their great potential to address several needs in the food industry, including: satiety control, encapsulation of phytonutrients and prebiotics, texture control for healthier food formulations (e.g. reduced fat products), and targeting delivery to specific areas in the digestive tract. We review the scientific and patent literature on the utilisation and manufacturing methods for producing microgels with an emphasis on micro-hydrogels for food applications.     

12—A CRITIQUE OF SOME HYPOTHESES RELATING TO THE HETEROGENEITY OF THE HIGH-SULPHUR PROTEINS OF WOOL

Joubert and Burns prepared a large number of fractions from the high-sulphur proteins of wool and estimated their molecular weights and amino-acid compositions. Their data have been re-examined in order to look for statistically significant interrelations between amino acids and between the proportion of various amino acids and molecular weight. Statistical analysis of the data is also used to examine the credibility of some hypotheses concerning the mechanism of keratin biosynthesis and to provide further evidence for the existence of families of proteins within the high-sulphur fractions of wool.     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of     

The Latest Data about Development of Chinese Printing Industry in 2013

In the 2014 China（Shanghai）International Printing Week,Director Wang Yanbin released the latest data about development of Chinese printing industry in 2013.According to statistics,in 2013,the total output value of Chinese printing industry exceeded 1trillion Yuan for the first time,reaching 1.03985 trillion Yuan.There were 105,000 printing enterprises in China,employees were 3.415 million.The total asset was 1.06247 trillion Yuan;     

The State Council Issued "Directory of Government Approved Investment Projects(2013 Edition)"

正On December 2nd,2013,the State Council issued the notification of"Directory of Government Approved Investment Projects(2013 Edition)"(hereafter referred to as"notification").It is pointed out in the"notification"that in order to further deepen reforms in investment systems and administrative examination and approval systems,simplify administrative procedures and delegate powers to lower levels,earnestly     

Top Ten News of China's Paper Industry 2013

On December 24th, 2013, the meeting on the selection of top 10 news of China＇s paper industry 2013 sponsored by 〈China Paper Newsletters〉 was held in Beijing. The yearly selection of the top l0 news, which began in 2000, has become a brand activity widely recognized in the industry thanks to the support from the authorities at all levels and public participation.     

National Development and Reform Commission Issued Announcement for Selection of Major Preliminary Research Projects for the "13~(th) Five-Year Plan"

In Apri Commi major p Plan＂ （h 2014, the National Development and Reform ssion issued an announcement for selection of reliminary research projects for the ＂13th Five-Year ereafter referred to as ＂The Announcement＂）     

Announcing a new international peer-reviewed journal:Food Science and Human Wellness now accepting manuscript submission in English

<正>We are pleased to announce the launch of a new international peer-reviewed journal-Food Science and Human Wellness,ISSN 2213-4530,which is an open access journal,produced and hosted by Elsevier B.V.on behalf of Beijing Academy of Food Sciences.Food Science and Human Wellness is an international peer-reviewed English journal that provides a forum for the dissemination of the latest scientific     

China textile machinery: taking off in progress

正Among the 1600 exhibitors who take apart in the ITMA ASIA+CITME2014 2/3 are Chinese manufactures.If the numerous figures failed to attract your attention,the increase of quality should draw your focus.To adopt the demand of developing textile machine market,domestic textile machinery enterprises now follow the slogan of"technology drives development"to enhance product competitiveness.Our domestic sellers will showcase product ranging from spinning,weaving,dyeing and printing,     

An internet compendium of analytical methods and spectroscopic information for monomers and additives used in food packaging plastics

An internet website (http://cpf.jrc.it/smt/) has been produced as a means of dissemination of methods of analysis and supporting spectroscopic information on monomers and additives used for food contact materials (principally packaging). The site which is aimed primarily at assisting food control laboratories in the European Union contains analytical information on monomers, starting substances and additives used in the manufacture of plastics materials. A searchable index is provided giving PM and CAS numbers for each of 255 substances. For each substance a data sheet gives regulatory information, chemical structures, physico-chemical information and background information on the use of the substance in particular plastics, and the food packaging applications. For monomers and starting substances (155 compounds) the infra-red and mass spectra are provided, and for additives (100 compounds); additionally proton NMR are available for about 50% of the entries. Where analytical methods have been developed for determining these substances as residual amounts in plastics or as trace amounts in food simulants these methods are also on the website. All information is provided in portable document file (PDF) format which means that high quality copies can be readily printed, using freely available Adobe Acrobat Reader software. The website will in future be maintained and up-dated by the European Commission's Joint Research Centre (JRC) as new substances are authorized for use by the European Commission (DG-ENTR formerly DGIII). Where analytical laboratories (food control or other) require reference substances these can be obtained free-ofcharge from a reference collection housed at the JRC and maintained in conjunction with this website compendium.