软饮料中糖精的快速分光光度测定法

糖精是一种人工合成甜味剂,广泛地应用于各种食品方面。食品饮料中糖精的测定已有很多方法,目前所使用重量法、极谱法、薄层色谱法、气—液色谱法、离子选择电极法、红外光谱法、紫外光谱法、分子发射腔分析法都比较繁琐、费时。用高压液相色谱法测定糖精,虽应用很广、操作简便。     

乳脂中1,3-甘油二酯检测方法的研究

建立一种乳脂中1,3-甘油二酯(1,3-DG)定性定量的检测方法.通过薄层色谱法(TLC)对乳脂中1,3-甘油二酯进行分离和定性,再用甘油氧化酶法进行定量检测.研究结果显示:薄层色谱法能有效分离出1,3-甘油二酯;定量方法RSD小于等于3.78%,回收率在86.73%～101.83%之间.该方法能准确、快速、灵敏地分离检测乳脂中的1,3-甘油二酯.     

玫瑰花中花色苷的分析方法研究

目的研究玫瑰花中花色苷的定性定量分析方法。方法采用薄层色谱法，硅胶G薄层板，正丁醇-醋酸-水（4：1：2）为展开剂，1％三氯化铝乙醇溶液为显色剂，检测矢车菊素-3，5-二葡萄糖苷；采用双波长pH示差法，测定总花色苷含量。结果薄层色谱法可清晰检出矢车菊素-3，5-二葡萄糖苷；双波长pH示差法测定结果的相对标准偏差为2．5％（n=6）。结论建立了玫瑰花中花色苷类成分的定性定量分析方法，可作为玫瑰花药材的质量控制方法。     

近代仪器分析在乳品工业中的应用

介绍了原子荧光光谱法、红外光谱法、核磁共振波谱法、旋光法、质谱法、气相色谱法、极谱法、高效液相色谱法、热分析法等近代仪器分析手段，及其在乳品工业中的应用。     

柱色谱分离提纯三氯蔗糖

采用薄层色谱法定性，以柱色谱法分离提纯三氯蔗糖。三氯蔗糖的平均回收率为95．18％，相对标准偏差为0．75％。并用红外光谱、质谱、核磁共振、元素分析等方法对三氯蔗糖的结构进行了鉴定。     

苯甲酸和糖精的极谱分析

苯甲酸和糖精的极谱分析王中一沈园新疆乌鲁木齐市卫生防疫站（８３０００２）苯甲酸和糖精是常用的食品添加剂，目前多用薄层色谱法，紫外分光光度法，气相和液相色谱法等进行定量。［１］本文利用糖精和消化后的苯甲酸在适当的介质中能产生灵敏的导数波而进行单扫描极谱...     

小儿积散质量标准的研究

采用薄层色谱法对处方中的木香、甘草进行定性鉴别;用高效液相色谱法测定制剂中甘草的含量,采用C18色谱柱,流动相为乙腈-0.1%磷酸溶液（38：62）,检测波长为250nm。结果表明：薄层鉴别色谱斑点清晰,阴性对照无干扰,专属性强;甘草酸在0.56～5.6μg范围内呈良好的线性关系,Y=3.93×105S-3.34×104r=0.9999。平均加样回收率为99.55%,RSD=0.56%（n=5）。建立的定性定量方法简便、准确、专属性强,可作为制剂的质量控制方法之一。     

地沟油检测方法的研究进展

地沟油是质量极差的非食用油,若用于食用会严重危害人的身体健康。介绍地沟油的分类、危害以及对地沟油的检测。尤其着重介绍了仪器分析在地沟油检测中的应用,包括电化学分析法、气质联用分析法、高效液相色谱法、离子色谱法、薄层色谱法、原子吸收法、紫外分光光度法、荧光法、红外光谱法、免疫分析法等。     

火锅底料中固体石蜡的定性检测方法研究

根据石蜡与食用油脂（主要是牛羊油）性质的差异，采用了各种定性检测方法对火锅底料中的石蜡定性检测进行了一系列研究。研究表明：采用物理化学手段，如薄层色谱法、皂化法、溶剂溶解法、利用DSC测定其熔点范围以及硬度曲线的测定对火锅底料中提取出来的油样均可以进行定性分析，确定其中有无含有石蜡。其中综合实施难度及仪器投资，以皂化法用于定性检测最为简便、快捷，可以在实际的市场监管中加以运用。     

柱色谱分离提纯蔗糖-6-乙酸酯

采用薄层色谱法定性 ,以柱色谱法分离提纯蔗糖 6 乙酸酯。蔗糖 6 乙酸酯的平均回收率为 95 0 6 % ,相对标准偏差为 1 0 5 %。并用红外光谱、质谱、核磁共振等方法对蔗糖 6 乙酸酯的结构进行了鉴定     

Measurements of lycopene contents in fruit: A review of recent developments in conventional and novel techniques

Lycopene is a biologically active phytochemical reported in fruit. Conventional techniques such as high performance liquid chromatography (HPLC) and thin layer chromatography (TLC) have been in existence for measuring lycopene in fruit, but these methods are destructive with relative accuracy and speed. Other novel spectroscopic and imaging approaches, which are more reliable and fast, have recently been developed to investigate complex components such as lycopene, total soluble solids, etc. in fruit. The current review attempts to highlight the potential of both conventional and novel techniques in evaluating lycopene contents of fruit. The novel techniques include both spectroscopic methods such as near infrared spectroscopy and Raman spectroscopy and spectral imaging approaches such as multispectral imaging, hyperspectral imaging and Raman imaging. The principles of these techniques are summarized, their detailed applications are discussed, and future trends are also presented. Both traditional and novel techniques highlighted in the current review can be used for assessing the distribution and concentration of lycopene in various fruit. Although novel spectroscopic and spectral imaging approaches may in the near future replace conventional methods, because conventional methods are typically often offline, destructive and time-consuming, which also require the use of chemicals.     

偶氮染料中微量芳香胺类的分析方法现状

为适应德国政府颁布的环保新法规的需要,作者介绍了国内外有关部门如何检测偶氮染料中微量芳香胺的方法。芳香胺的分析方法有化学分析法、分光光度法、薄层色谱法、气相色谱法、气相色谱/质谱联用法、液相色谱法和液相色谱/质谱联用法等。     

栀子黄A_(238nm)/A_(440nm)值与西红花苷和栀子苷相关性研究

目的:采用TLC、HPLC和紫外分光光度法(UV)研究栀子黄OD值(栀子黄中栀子苷的最大吸光度A238nm与西红花苷的最大吸光度A440nm的比值)与栀子苷含量的关系。方法:采用硅胶柱层析从栀子中分离西红花苷纯品,然后分别采用TLC、HPLC和UV来定性和定量分析栀子乙醇提取部位和经大孔吸附树脂精制的栀子黄,并计算波长238nm和440nm处的百分比吸光系数及OD值。结果:经过对以上数据的分析,发现西红花苷本身在波长238nm处有明显紫外吸收,采用OD值来控制栀子黄中的栀子苷将高估栀子苷含量。结论:栀子黄中栀子苷的控制不宜采用OD值作为评价指标,而应采用具有分离功能的高效液相色谱法来作为质量控制的手段。     

紫杉醇的薄层层析显色反应与应用

为了进行抗癌药物紫杉醇产生菌株的快速筛选,建立了紫杉醇检测的薄层层析(TLC)方法。研究结果表明,应用薄层层析技术对内生真菌的发酵产物进行检测比高效液相色谱(HPLC)方法更加高效,能够简便、准确的筛选到紫杉醇产生菌株,并且可以初步推测菌株的紫杉醇产生能力。     

Total Sterols in Seafoods: Iatroscan TLC/FID Versus the Kovacs GLC/FID Method

THE TOTAL STEROLS of selected seafood samples were determined, after saponification, by latroscan TLC/FID and the results were compared with those obtained by the more common technique of gas-liquid chromatography which gives individual sterols. No significant difference was found between the techniques in terms of quantitative accuracy for total sterols. In many products the sterol compositions were known or specific compositional details were not important. Thus, in most finfish and Crustacea species, cholesterol constituted over 90% of the sterols and, therefore, the Chromarodlatroscan TLC/FID with or without inclusion of an internal standard would give an accurate estimation of the cholesterol in fish products.     

Mycotoxin analysis: an update

Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.     

Mycotoxin analysis: An update

Mycotoxin contamination of cereals and related products used for feed can cause intoxication, especially in farm animals. Therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current methods usually include an extraction step, a clean-up step to reduce or eliminate unwanted co-extracted matrix components and a separation step with suitably specific detection ability. Quantitative methods of analysis for most mycotoxins use immunoaffinity clean-up with high-performance liquid chromatography (HPLC) separation in combination with UV and/or fluorescence detection. Screening of samples contaminated with mycotoxins is frequently performed by thin layer chromatography (TLC), which yields qualitative or semi-quantitative results. Nowadays, enzyme-linked immunosorbent assays (ELISA) are often used for rapid screening. A number of promising methods, such as fluorescence polarization immunoassays, dipsticks, and even newer methods such as biosensors and non-invasive techniques based on infrared spectroscopy, have shown great potential for mycotoxin analysis. Currently, there is a strong trend towards the use of multi-mycotoxin methods for the simultaneous analysis of several of the important Fusarium mycotoxins, which is best achieved by LC-MS/MS (liquid chromatography with tandem mass spectrometry). This review focuses on recent developments in the determination of mycotoxins with a special emphasis on LC-MS/MS and emerging rapid methods.     

肉品检测技术的研究进展

目前肉类食品的检验正朝着更加安全和卫生的方向发展,检验质量不断提高。该文介绍了当代肉类品质检测技术,包括物理分析法中的计算机视觉技术、超声波技术、电磁学检测技术;仪器分析法的高效液相色谱、毛细管电泳安培法、近红外光谱分析技术、核磁共振波谱分析技术;现代分子生物学技术的核酸探针检测技术、生物芯片检测技术和微生物快速检验方法。     

食用油脂中矿物油的定性检测方法研究

研究了皂化法、薄层色谱法及棒状薄层色谱-氢火焰离子化检测器(TLC-FID)法检测食用油脂中的矿物油。结果表明:皂化法简便、快捷,最低检测限可达0.5%,但不适用于本身含蜡质的食用油脂(如米糠油、玉米油及葵花油等);薄层色谱法(TLC)同样可对食用油脂中的矿物油进行定性检测,最低检测限可达到0.2%左右,但仍不适用于本身含蜡质的食用油脂;TLC-FID法克服了以上两种方法的缺陷,不仅适用于本身不含蜡质的食用油脂,还可对本身含蜡质的食用油脂进行矿物油的定性检测,不会出现误检,相比之下更具有优越性。     

Gangliosides in human milk and infant formula: A review on analytical techniques and contents

Gangliosides (GGs) are important bioactive compounds that offer beneficial anti-infective, anti-inflammatory, and neuronal development effects. The present work reviews the techniques used to determine the GG content in human milk and infant formula. In the case of conventional techniques (thin-layer chromatography (TLC) and spectroscopy), extraction and purification steps are more laborious than for the current techniques (liquid chromatography-mass spectrometry (LC-MS)). The new methodologies allow the identification of the ceramide and oligosaccharide forming the GGs, which is of interest considering their structural differences among human milk and infant formula. This information could be used to incorporate GGs into infant formula in such a way as to more closely resemble human milk regarding total contents and profile.