共查询到19条相似文献，搜索用时 46 毫秒
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随着生物医学技术的发展,组织样本经常被多种荧光标记物标记,需要通过光谱成像的方法区分出样本中不同的成分。本文在共聚焦显微镜基础上,介绍了一种由精密丝杠和步进电机控制的狭缝机构实现光谱成像的方法,讨论了狭缝缝片的具体设计和狭缝运动精度对光谱带宽和波长准确度的影响。 相似文献
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激光扫描共聚焦显微镜技术在材料学研究中的应用 总被引：1，自引：0，他引：1
激光扫描共聚焦显微镜技术(laser scanning confocal microscopy,LSCM)有效地排除了非焦平面信息,提高了分辨率和对比度,使图像更为精确清晰,与计算机及相应的软件技术组合,LSCM实现了连续光学切片,广泛应用于生物三维结构重组及动态分析.LSCM是一种无损深层形态结构分析的重要方法,可以对材料进行深层形貌观察;本文主要综述了LSCM的成像原理以及LSCM在高分子材料和生物工程材料方面的应用. 相似文献
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激光扫描共聚焦显微镜技术的发展及应用 总被引：8，自引：1，他引：8
激光扫描共聚焦显微术是先进的分子和细胞生物学研究技术。它在荧光显微镜成像的基础上加装激光扫描装置,结合数据化图像处理技术,采集组织和细胞内荧光标记图像。在亚细胞水平观察钙等离子水平的变化,并结合电生理等技术观察细胞生理活动与细胞形态及运动变化的相互关系。由于它的应用范围较广泛,已成为形态学、分子细胞生物学、神经科学和药理学等研究领域中很重要的研究技术。 相似文献
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介绍了一种光纤共聚焦显微内窥镜实时活体内成像系统.该系统采用高速扫描振镜、超细成像光纤束、大尺度几何形变理论图像拼接技术,并结合自主研发的综合软件,使该系统具有实时检测、探头物理尺寸小、图像分辨力高、用户界面友好、操作方便等多方面优势.实验表明:该系统可为医生提供丰富的组织学和病理学影像信息,提高诊断准确率.该系统为临床医学提供了一种能在活体内进行实时细胞尺寸检测的医疗仪器,是癌症早期诊断的重要工具. 相似文献
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脱氧核糖核酸(DNA)是染色体的重要组成部分,是一种主要的遗传物质,很多实验方法与理论模型被用来研究DNA与一些重要生物物种相互作用引起的构象变化。采用激光扫描共聚焦表面等离子体共振(LSCI-SPR)系统研究了一种特定单链DNA(ssDNA)分别与其互补DNA(cDNA)和汞离子(Hg~(2+))作用后的构象变化。将一端有荧光分子的ssDNA修饰在表面等离子体共振系统传感芯片上,通过观察荧光图像的变化来确定ssDNA与cDNA及Hg~(2+)之间相互作用而导致的构象变化,并通过动力学曲线计算出二者的结合速率分别为3.33×10~(-5) s~(-1)和1.42×10~(-4) s~(-1)。对于ssDNA-cDNA的相互作用,荧光图像没有变化。对于ssDNA-Hg~(2+)的相互作用,在通入Hg~(2+)后荧光发生猝灭。这些结果表明,激光扫描共聚焦表面等离子体共振系统在高灵敏实时监测ssDNA与其他特殊生物分子作用产生的构象变化和计算动力学参数方面有着广阔的应用前景。 相似文献
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扼要的介绍了激光扫描显微镜在国内外的发展状况,着重介绍了激光扫描显微镜中特殊成像功能OBIC成像的测量方法、基本原理、技术特色和应用前景。 相似文献
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Three-dimensional (3-D) imaging in confocal microscopes is considered in terms of 3-D transfer functions. This leads to an explanation of axial imaging properties. The axial response was observed in both object-scanning and beam-scanning microscopes and the influence of off-axis examination investigated. By simple processing of multi-detector signals, imaging in both the axial and transverse directions can be improved. 相似文献
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A small diameter (600 µm) fused optic fibre imaging bundle was used as a probe to compare fluorescent specimens by direct contact imaging using both a conventional fluorescence microscope and a laser scanning confocal microscope (LSCM) system. Green fluorescent polyester fibres placed on a green fluorescent cardboard background were used to model biological tissue. Axial displacement curves support the hypothesis that pinhole size in the LSCM system reduces the contribution of non‐focal plane light. Qualitative comparison showed that the LSCM system produced superior image quality and contrast over the conventional system. The results indicate that the new LSCM–probe combination is an improvement over conventional fluorescence–probe systems. This study shows the feasibility of employing such a small diameter probe in the investigation of biological function in difficult to access areas. 相似文献
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In common light microscopy, observation of samples is only possible from one perspective. However, especially for larger three‐dimensional specimens observation from different views is desirable. Therefore, we are presenting a sample holder permitting rotation of the specimen around an axis perpendicular to the light path of the microscope. Thus, images can be put into a defined multidimensional context, enabling reliable three‐dimensional reconstructions. The device can be easily adapted to a great variety of common light microscopes and is suitable for various applications in science, education and industry, where the observation of three‐dimensional specimens is essential. Fluorescence z‐projection images of copepods and ixodidae ticks at different rotation angles obtained by confocal laser scanning microscopy and light sheet fluorescence microscopy are reported as representative results. 相似文献
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An inverted fluorescence microscope was upgraded into a compact three-dimensional laser scanning microscope (LSM) of 65 x 62 x 48 cm dimensions by means of a fast kHz galvoscanner unit, a piezodriven z-stage, and a picosecond (ps) 50 MHz laser diode at 405 nm. In addition, compact turn-key near infrared femtosecond lasers have been employed to perform multiphoton fluorescence and second harmonic generation (SHG) microscopy. To expand the features of the compact LSM, a time-correlated single photon counting unit as well as a Sagnac interferometer have been added to realize fluorescence lifetime imaging (FLIM) and spectral imaging. Using this unique five-dimensional microscope, TauMap, single-photon excited (SPE), and two-photon excited (TPE) cellular fluorescence as well as intratissue autofluorescence of water plant leaves have been investigated with submicron spatial resolution, <270 ps temporal resolution, and 10 nm spectral resolution. 相似文献
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The phosphatized microfossils from Doushantuo Formation, Southeast China show us the biodiversity about 600 million years ago, which is a unique window for the evolution of the early life on earth. However, the process of phosphatic fossilization in detail still remains unknown. Here we report our study on the preservation state of the fossils by using confocal laser scanning microscopy. We found that fluorescent signal of the fossil could reflect the preservation state when compared with the transmission light microscopy. First, we found the fluorescent signal of the decayed cells of the fossil was weaker than that of the nondecayed part. Second, we found that the three-dimensional reconstruction of the fluorescent signals could help to judge the degree of mineralization of the fossil cells, compared with the observation by transmission light microscope. Third, we found that almost all of the fossil specimens we observed could fluoresce more or less when excited by laser light. Therefore, the fluorescent microscopy provides a useful method for the study of the preservation state of the phosphatic fossil cells. 相似文献
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Zden k Petr&#x;ek Juan M. Bolivar Bernd Nidetzky 《Microscopy research and technique》2021,84(1):71-78
We determine the optimal parameters (scan velocities) for measuring the luminescence lifetime on the microsecond scale using the recently introduced method based on scanning the excitation beam across the sample. Using simulations, we evaluate the standard deviation and bias of the luminescence decay rate determined by scanning with two different velocities. The analysis is performed for Poisson‐ and normal‐distributed signals, representing different types of detection techniques. We also show that a weak uncorrected background induces a bias in the obtained decay rate, and take this effect into account when choosing optimal measurement parameters. For comparison, the analysis is additionally performed for two conventional gating schemes for lifetime measurement. The variable‐velocity scanning method is found to be more robust to the effect of the background signal than the gating schemes. 相似文献
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Imaging of cells in two dimensions is routinely performed within cell biology and tissue engineering laboratories. When biology moves into three dimensions imaging becomes more challenging, especially when multiple cell types are used. This review compares imaging techniques used regularly in our laboratory in the culture of cells in both two and three dimensions. The techniques reviewed include phase contrast microscopy, fluorescent microscopy, confocal laser scanning microscopy, electron microscopy, and optical coherence tomography. We compare these techniques to the current \"gold standard\" for imaging three-dimensional tissue engineered constructs, histology. 相似文献
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Protein body-free maize zein, when mixed at 35 degrees C (above its glass transition temperature range), significantly (p < 0.01) improved the rheological and leavening properties of sorghum-wheat composite flour dough, resulting in improved loaf volume. Confocal laser scanning microscopy was used to observe the structure of zein fibrils and the interaction between zein and gluten proteins in the composite dough and bread systems. Autofluorescence and immunolocalization techniques were used to locate gluten and zein, respectively. Optical sections were collected every 0.4 microm through the samples and digitally processed to produce reconstructed three-dimensional images. Results showed that zein fibrils form an outer layer that intermittently coats the gluten networks, thereby strengthening them. This type of microstructure is able to withstand the pressure exerted by gas cell expansion during yeast fermentation to increase loaf volume. 相似文献