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排序方式: 共有211条查询结果,搜索用时 31 毫秒
1.
C. A. Pineda P. Wenzl J. Mayer J. Mesjasz-Przybylowicz W. J. Przybylowicz V. M. Prozesky 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》1997,130(1-4):362-367
A Brachiaria breeding project is being conducted at CIAT, Colombia to combine acid soil adaptation of B. decumbens with other favourable traits of Brachiaria species that are less adapted to acid soils. Micro-PIXE was applied to investigate nutrient uptake and distribution in root tips of different species grown in hydroponic culture under control and simulated acid soil stress conditions. Different sample measurement approaches were evaluated, including: (1) linear scans with single point measurements along the root axis; (2) mapping of whole root tips; and (3) mapping of root cross sections. Different tissue types could be distinguished on the base of differences in nutrient concentrations and/or Al stress. Al, if supplied under nutrient stress conditions, increased P accumulation in the central vascular tissue of the meristematic and elongation zone of B. decumbens and B. brizantha. Furthermore, a negative correlation was found between Al and Cl accumulation in the root cap of B. decumbens. 相似文献
2.
Baeyer-Villiger monooxygenases (BVMOs) are enzymes able to perform highly regio-plus steroselective nucleophilic and electrophilic biooxygenations on various substrates. The resultant chiral products (lactones and sulfoxides) can be valuable for the chemoenzymatic synthesis of a wide range of useful compounds. Recent studies have provided a number of alternative active-site models that attempt to explain the exquisite and unusual selectivity of BVMOs. This article reviews some of the established applications, and considers the merits of the various predictive models. 相似文献
3.
K. V. Sudhakar J. C. Cisneros Hector Cervantes Cosme Gomez Pineda 《Journal of Materials Engineering and Performance》2006,15(1):117-121
The technology of materials removal is improved greatly by the introduction of advanced cutting tools like cubic boron nitride,
ceramics, polycrystalline diamond and the more recent whisker-reinforced materials. In this paper, the influence of cutting
temperature on machinability, mechanical properties, microstructure, and fracture morphology of Cu-2Be alloy using a polycrystalline
diamond cutter is investigated. The information on machining, microstructure, and fracture morphology of Cu-2Be alloy are
very useful to understand their fabrication characteristics and the basic mechanisms of its deformation and fracture. The
machinability (in terms of surface finish) of Cu-2Be alloy is evaluated as a function of cutting temperature, resulting from
wet and dry cutting. Machining is carried out on a Hardinge Cobra 42 CNC machine (Hardinge Inc., Elmira, NY), and the machining
parameters used—cutting speed, depth of cut, and feed rate—are kept constant during both wet and dry cutting. The machined
surface finish on Cu-2Be alloy is measured using a surface finish analyzer (Surftest 401, series 178) technique. The machined
specimens are examined for their strength and hardness properties using a standard Universal Testing Machine and Rockwell
hardness tester, respectively. Wet cutting (using coolants) produced a smooth surface finish when compared with dry cutting
of the Cu-2Be alloy. The machined specimens are examined for their microstructural features using a Nikon optical microscope.
The specimens are etched using a suitable etchant solution for revealing such microstructure constituents as grain size, phase
proportions, and the possible overheated areas (especially in dry cutting). The fractured surfaces from the tensile and impact
toughness tests are investigated for their fracture morphologies (dry and wet cutting) using a microprocessor-controlled scanning
electron microscope (Jeol Model JSM 5910 LV). A detailed analysis is also made to understand and interpret the basic fracture
mechanisms responsible for crack initiation and crack propagation. The Cu-2Be alloy showed relatively higher mechanical properties
in wet cutting in comparison to dry cutting operations. Fracture studies demonstrated intergranular and ductile fractures
as dominant modes of fracture mechanisms in Cu-2Be alloy. 相似文献
4.
Many optimization models in engineering are formulated as bilevel problems. Bilevel optimization problems are mathematical programs where a subset of variables is constrained to be an optimal solution of another mathematical program. Due to the lack of optimization software that can directly handle and solve bilevel problems, most existing solution methods reformulate the bilevel problem as a mathematical program with complementarity conditions (MPCC) by replacing the lower-level problem with its necessary and sufficient optimality conditions. MPCCs are single-level non-convex optimization problems that do not satisfy the standard constraint qualifications and therefore, nonlinear solvers may fail to provide even local optimal solutions. In this paper we propose a method that first solves iteratively a set of regularized MPCCs using an off-the-shelf nonlinear solver to find a local optimal solution. Local optimal information is then used to reduce the computational burden of solving the Fortuny-Amat reformulation of the MPCC to global optimality using off-the-shelf mixed-integer solvers. This method is tested using a wide range of randomly generated examples. The results show that our method outperforms existing general-purpose methods in terms of computational burden and global optimality. 相似文献
5.
6.
Sequential learning is the discipline of machine learning that deals with dependent data such that neighboring labels exhibit some kind of relationship. The paper main contribution is two-fold: first, we generalize the stacked sequential learning, highlighting the key role of neighboring interactions modeling. Second, we propose an effective and efficient way of capturing and exploiting sequential correlations that takes into account long-range interactions. We tested the method on two tasks: text lines classification and image pixel classification. Results on these tasks clearly show that our approach outperforms the standard stacked sequential learning as well as state-of-the-art conditional random fields. 相似文献
7.
Jon Luzuriaga Yurena Polo Oier Pastor-Alonso Beatriz Pardo-Rodríguez Aitor Larraaga Fernando Unda Jose-Ramon Sarasua Jose Ramon Pineda Gaskon Ibarretxe 《International journal of molecular sciences》2021,22(7)
Human dental pulp stem cells (hDPSCs) are some of the most promising stem cell types for regenerative therapies given their ability to grow in the absence of serum and their realistic possibility to be used in autologous grafts. In this review, we describe the particular advantages of hDPSCs for neuroregenerative cell therapies. We thoroughly discuss the knowledge about their embryonic origin and characteristics of their postnatal niche, as well as the current status of cell culture protocols to maximize their multilineage differentiation potential, highlighting some common issues when assessing neuronal differentiation fates of hDPSCs. We also review the recent progress on neuroprotective and immunomodulatory capacity of hDPSCs and their secreted extracellular vesicles, as well as their combination with scaffold materials to improve their functional integration on the injured central nervous system (CNS) and peripheral nervous system (PNS). Finally, we offer some perspectives on the current and possible future applications of hDPSCs in neuroregenerative cell therapies. 相似文献
8.
Angela Pineda Jonathan CarrascoCarlos Peña-Farfal Karem Henríquez-AedoMario Aranda 《Food Control》2012,23(1):251-257
Biogenic amines are organic nitrogenous compounds produced in wines mainly during malolactic fermentation. Its presence is a health risk for sensitive individuals and it could negatively affect the wine quality. The objective of this work was to determine the biogenic amines content in Chilean young varietal wines. For that, a liquid chromatographic method with pre-column derivatization was optimized and validated to achieve a reliable quantification of the most important biogenic amines in wines, i.e. histamine, tyramine, spermine, spermidine, putrescine, cadaverine and phenylethylamine. Using a face-centered central composite design derivatization and chromatographic conditions were optimized. Derivatization with dansyl chloride showed an optimum yield for all biogenic amines at 60 °C during 60 min. These derivatives were optimally separated with a C18 YMC-Pack ODS-A column set a 40 °C using a mobile phase composed of acetonitrile and water under gradient conditions. An effective separation and quantification of the biogenic amines was achieved in 40 min using ultraviolet detection at 254 nm. Validation was established according to ICH guidelines, calibrations in matrix fitted a linear regression model with R2 > 0.997. Repeatability (n = 6) and intermediate precision (n = 3) in matrix showed RSD values <2.34% and <3.16%, respectively. This method was applied to determine for the first time the biogenic amines content in Chilean wines. Twenty-seven samples of young varietal wines, i.e. Cabernet Sauvignon (n = 9), Merlot (n = 8) and Carménère (n = 10) were analyzed finding a biogenic amines content from 18.12 to 39.84 mg L-1, where putrescine showed the highest concentration in all grape varieties. No significant difference was found between grapes varieties regarding total biogenic amines content (P = 0.7842, df = 26). Along with putrescine, histamine and tyramine were the most abundant biogenic amines but similar levels were also observed with spermidine. 相似文献
9.
Sanghee Lee Theresa R. Mendoza Danielle N. Burner Michelle T. Muldong Christina C. N. Wu Catalina Arreola-Villanueva Abril Zuniga Olga Greenburg William Y. Zhu Jamillah Murtadha Evodie Koutouan Naomi Pineda Hao Pham Sung-Gu Kang Hyun Tae Kim Gabriel Pineda Kathleen M. Lennon Nicholas A. Cacalano Catriona H. M. Jamieson Christopher J. Kane Anna A. Kulidjian Terry Gaasterland Christina A. M. Jamieson 《International journal of molecular sciences》2022,23(6)
10.
Miriam Prez-Saldívar Graciela Ordoez Benjamín Pineda Julio Sotelo Adolfo Martínez-Palomo Jos Flores-Rivera Martha Espinosa-Cantellano 《International journal of molecular sciences》2022,23(1)
An association between varicella zoster virus (VZV) and multiple sclerosis (MS) has been reported in Mexican populations. The aim of this study was to compare the response of T cells from MS patients, during relapse and remission, to in vitro stimulation with VZV, adenovirus (AV) and Epstein–Barr virus (EBV). Proliferation and cytokine secretion of T cells from 29 relapsing-remitting MS patients and 38 healthy controls (HC) were analyzed by flow cytometry after stimulating with VZV, AV or EBV. IgG and IgM levels against VZV and EBV were quantified using Enzyme-Linked Immunosorbent Assay. Relapsing MS patients showed a higher percentage of responding CD4+ and CD8+ T cells against VZV compared to AV. In HC and remitting MS patients, proliferation of CD4+ T cells was higher when stimulated with VZV as compared to EBV. Moreover, T cells isolated from remitting patients secreted predominantly Th1 cytokines when cell cultures were stimulated with VZV. Finally, high concentration of anti-VZV IgG was found in sera from patients and controls. The results support previous studies of an VZV-MS association in the particular population studied and provide additional information about the possible role of this virus in the pathogenesis of MS. 相似文献