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排序方式: 共有593条查询结果,搜索用时 797 毫秒
1.
Dr. Emrah Kara Dr. Nis Valentin Nielsen Bergrun Eggertsdottir Dr. Bernd Thiede Dr. Sandip M. Kanse Dr. Geir Åge Løset 《Chembiochem : a European journal of chemical biology》2020,21(13):1875-1884
We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated. 相似文献
2.
抽水蓄能电站运行方式和电网经济效益分析 总被引:1,自引:1,他引:0
用计算机模拟法建立模拟模型,对选定的系统进行生产模拟,统计计算出电网系统投入不同抽水蓄能容量时所发生的系统运行总燃料费用(包括抽水蓄能电站的抽水费)和系统的负荷缺电力时间概率(LOLP)等指标。通过对计算结果的分析,得出不同系统在投入不同容量抽水蓄能机组和采取不同运行方式的情况下,其运行总燃料费用的边际成本和LOLP的边际值的变化规律,并且对电网的经济效益进行合理分析。从而为抽水蓄能电站的规划和投资建设提供理论依据,推动抽水蓄能电站的发展,促使我国电源结构趋向合理。 相似文献
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介绍了非稳态法测试液体在低温下(-100~50℃)的热导率的测试原理、方法,测试了全氟三乙胺的低温热导率。为了提高测试的重复性,采用了相对比较法,并给出了测试装置及测试结果和误差分析。 相似文献
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一种高性能超宽带线性调频信号源 总被引:3,自引:0,他引:3
介绍一种VHF/UHF频段高性能超宽带线性调频信号源的设计与实现。首先简要阐述了高性能超宽带线性调频信号源的设计思想,然后依据基带数字产生结合倍频扩展带宽的思路提出了一种超宽带线性调频信号产生方案,并进行了设计实现研究。测试结果表明:按照本文阐述的指导思想和实现方案所设计的超宽带线性调频信号源达到 了相当高的性能。 相似文献
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Monika Backhaus-Ricoult Serge Hagège Aurélie Peyrot Patrick Moreau 《Journal of the American Ceramic Society》1994,77(2):423-430
(Al,Cr)2 O3 single crystals and polycrystals were internally reduced at 1873 K in an Al/Al2 O3 buffer for periods of time ranging from 1 to 100 h. The growth kinetics of the reduction scale were measured. The microstructure of the reduction scale was investigated by SEM and TEM. As a result of the reduction, two types of discrete chromium precipitates developed inside the alumina matrix (inside the single crystal or the polycrystalline grains), each one being characterized by a particular morphology (needle or spheroid) and a low-energy orientation relationship with respect to the alumina matrix. In addition, larger precipitates without special orientation relationship developed along the grain boundaries and at the triple junctions of the polycrystais. In the first part of this paper, the precipitate morphology and size are described in terms of the crystallography of the interface between the two crystal structures in relation to the reduction mechanism. In the second part, the global reduction scale growth is analyzed in terms of point defect fluxes across the reduction scale. 相似文献
9.
Flow stress and softening behavior of wrought magnesium alloy AZ31B at elevated temperature 总被引:3,自引:0,他引:3
1 INTRODUCTIONThewroughtmagnesiumalloyshaveexcellentspecificstrengthandstiffness ,machinability ,dampcapacity ,dimensionalstability ,lowmeltingcostsandare ,hence ,veryattractiveinsuchapplicationsasau tomobile ,aviation ,electronicandcommunicationin dustry[16 ] .Investigationsontheflowstressandsofteningbehaviorofmagnesiumalloysathigherformingtem peratureandstrainratehavebeenanimportantsub jectinwroughtmagnesiumalloysforming[710 ] .InthispapertheflowstressandsofteningbehaviorofAZ31Bdeform… 相似文献
10.
Sophie Lakard Nadège Morrand-Villeneuve Boris Lakard Guillaume Herlem Bernard Fahys 《Electrochimica acta》2007,53(3):1114-1126
Up to today, several techniques have been used to maintain cells in culture for studying many aspects of cell biology and physiology. More often, cell culture is dependent on proper anchorage of cells to the growth surface. Thus, poly-l-lysine, fibronectin or laminin are the most commonly used substrates. In this study, electrosynthesized biocompatible polymer films are proposed as an alternative to these standard substrates. The electrosynthesized polymers tested were polyethylenimine, polypropylenimine and polypyrrole. Then, the adhesion, proliferation and morphology of rat neuronal cell lines were investigated on these polymer substrates in an attempt to develop new and efficient polymer materials for cell culture. During their growth on the polymers, the evolution of the cell morphology was monitored using both confocal microscopy and immunohistochemistry, leading to the conclusion of a normal development. An estimation of the adhesion and proliferation rates of rat neuronal cell cultures indicated that polyethylenimine and polypropylenimine were the best substrates for culturing olfactory neuronal cells. A method to favour the differentiation of the neuronal cells was also developed since the final aim of this work is to develop a biosensor for odour detection using differentiated neuronal cells as transducers. Consequently, a biosensor was microfabricated using silicon technology. This microsystem allowed us to culture the cells on a silicon wafer and to position the cells on certain parts of the silicon wafer. 相似文献