Membrane based detection of genetically modified organisms in some representatives food

Recently, DNA-based techniques became very common for the detection of genetically modified organisms (GMOs) in food products. For rapid and easy detection of GMOs, polymerase chain reaction (PCR) screening methods, which amplify common transgenic elements, are applied in routine analysis. Incorporation of PCR and membrane method introduced in this study offer an alternative detection of GMOs. In this study, a total of 32 samples and three certified reference materials were tested for the existence of the 35S promoter of cauliflower mosaic virus (CaMV) and 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) gene residues. Dot blot screening system introduced in this study can be routinely used as a semi-quantitative screening of GMOs.     

不同品种甜菜类型甜菜光合特性的研究

水文研究了甜菜生育期中不同品种类型甜菜的光合特性，结果表明在生育前期，高糖型品种的叶绿素含量高，而生育中前期以丰产型高，标准型介于二者之间，ＲｕＢＰ竣化酶活性的动态变化亦显这种趋势；在叶丛形成期和块根增长期，丰产型品种光合速度最高，在糖分积累期则以高糖型品种光合速度高；不同品种不同叶位的叶绿素含量与光合速率中呈正相关。     

仓式静态连续好氧堆肥发酵工艺的水气研究

采用仓式静态连续好氧堆肥发酵工艺,通过建立水平衡体系,系统地研究了水分对整个发酵工艺的影响,得出了仓式静态连续好氧堆肥发酵具有工艺简便,热交换快,水气蒸发量大等特点;好氧堆肥发酵过程中发酵温度保持在65℃左右,含水量控制在45%～55%有利于有机物降解,垃圾堆肥可在40～50d腐熟;静态连续好氧一次堆肥发酵物料的含水量需控制在35%以下,机械自动分选(类)筛分效率高,筛分率在90%～95%,垃圾减容在50%～60%.二次堆肥发酵物料的含水量需控制在25%～30%,机械除杂(碎石、碎玻璃等)效果好,有机肥中杂质含量低于0.8%.静态好氧二次堆肥发酵可以用垃圾渗沥水补充水分,达到资源循环再利用,可实现污水零排放.     

Effects of main factors on remedying northern landscaping water by hybrid ecological filter

Natural zeolite and coal cinder were layered as main packing medium of the ecological filter instead of traditional filling to remedy the static lake water in Northern China.The ecological filter was running in a combined upward and downward flow mode.Dynamic experiments were carried out to study the effects of retention time and ambient temperature on pollutants’ removal efficiency of the hybrid ecological filter.The function of plant was also studied by contrast test.It is showed that the removal efficiencies of NH4+-N and TP are increased when the retention time is changed from 1 h to 2 h and 4 h,but the removal efficiency of TN is decreased,the removal efficiency of NH4+-N is increased from 91.5% to 98% and that of TP is increased from 31.8% to 52.5%.When the temperature declines,the temporal removal efficiency of NH4+-N is reduced,but the removal efficiency of 24 h and 48 h is remained.The removal efficiency of TP after 24 h and 48 h is decreased when the temperature declines evidently.The retention time plays an important role in NH4+-N and TP removal,and the ambient temperature is significant for TP removal.The plant favors for TP and organic matter removal but has little effect on TN removal.     

站内搜索系统动态摘要算法的研究

对搜索系统中的几种摘要算法进行了介绍,分析了lucene软件包中的动态摘要算法存在的问题,即没有充分考虑关键词在整篇文章中的重要性.然后提出了改进后的算法,考虑了关键词出现在文章的位置,上下文等相关因素.改进后的算法充分考虑了文档摘要的要求:一是能表达文档的主旨;二是"可读性"比较高.经测试改进后的算法生成文摘质量较好.     

1–MCP结合EA对蓝靛果贮藏品质及活性氧代谢的影响

目的 探究不同处理对贮藏0~60 d期间蓝靛果实贮藏品质及活性氧(ROS)代谢的影响,为蓝靛果贮藏保鲜提供技术依据。方法 以蓝靛果为实验材料,采后将其装入保鲜箱中,用1−甲基环丙烯(1-MCP)、乙烯吸收剂(EA)、1-MCP+EA进行处理,在(−0.5±0.3)℃下贮藏60 d,每隔15 d取样观察果实的感官品质,并测定其营养、生理及活性氧代谢相关指标。结果 与对照组相比,3种处理方式均能保持果实较好的感官特性,延缓果实抗坏血酸、花色苷、总酚和黄酮等含量的流失,以及果实的软化;在贮藏60 d时,处理组果实的呼吸强度分别比对照组果实的呼吸强度低22.73、12.92、34.04 mg/(kg.h),乙烯生成速率分别比对照组果实的低6.38、3.98、10.11 μL/(kg.h);可抑制超氧阴离子(O2⁻.)活性、过氧化氢(H2O2)含量、丙二醛含量及相对电导率的升高,保持较高的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性。通过SPSS分析可知,综合得分顺序为CK＜EA＜1-MCP＜1-MCP+EA,表明1-MCP+EA处理的效果最好。结论 1-MCP+EA处理对蓝靛果贮藏60 d的保鲜效果最好,可更好地保留果实的外观和内在品质,利于运输和销售。     

企业案例教学在“Linux项目实战”课程中的运用

文章针对高职学生培养与企业实际需要存在巨大差异的问题展开研究,并以我校网络技术专业"Linux项目实战"课程为例,探讨运用企业案例教学、训练学生实际工作经验来解决实际工作问题,实践证明,该教学法能帮助学生在校积累丰富经验、提高应用能力。     

Information diffusion-based spatio-temporal risk analysis of grassland fire disaster in northern China

This study presents a Geographical Information Systems (GIS) and information diffusion-based methodology for spatio-temporal risk analysis of grassland fire disaster to livestock production in the grassland area of the northern China. We employed information matrix to analyze and to quantify fuzzy relationship between the number of annual severe grassland fire disasters and annual burned area. We also evaluated the consequences of grassland fire disaster between 1991 and 2006 based on historical data from 12 northern China provinces. The results show that the probabilities of annual grassland fire disasters and annual damage rates on different levels increase gradually from southwest to northeast across the northern China. The annual burned area can be predicted effectively using the number of annual severe grassland fire disasters. The result shows reliability as tested by two-tailed Pearson correlation coefficient. This study contributes a reference in decision making for prevention of grassland fire disaster and for stockbreeding sustainable development planning. The fuzzy relationship could provide information to make compensation plan for the disaster affected area.     

计算机专业设置生物信息学课程的建设与实践

针对目前国内生物信息学教学现状,借鉴其他学科成熟的教学模式,结合生物信息学的课程特点,探讨我校在计算机专业中开设生物信息学课程的几个问题,并提出基本教学思路和具体应用方法。     

Stability analysis for discrete delayed Markovian jumping neural networks with partly unknown transition probabilities

This paper addresses the analysis problem of asymptotic stability for a class of uncertain neural networks with Markovian jumping parameters and time delays. The considered transition probabilities are assumed to be partially unknown. The parameter uncertainties are considered to be norm-bounded. A sufficient condition for the stability of the addressed neural networks is derived, which is expressed in terms of a set of linear matrix inequalities. A numerical example is given to verify the effectiveness of the developed results.