狂犬病疫苗脂质体冻干粉的免疫原性评价

目的评价狂犬病疫苗脂质体冻干粉的免疫原性。方法将狂犬病疫苗原液稀释至15 IU/mL,制备为稀释液冻干粉(Y1组);将该稀释液冻干粉与脂质体冻干粉按5∶3的体积比混合,制成物理混合物(Y2组);同时采用冻融-冻干法将狂犬病疫苗稀释液和脂质体配制成狂犬病疫苗脂质体冻干粉(Y3组)。将各组疫苗复溶后,分别于0、3、7、14、21 d经小鼠腹腔给药,0. 5 mL/只。MTT法检测小鼠脾淋巴细胞增殖能力,流式细胞术检测T淋巴细胞表面标记,ELISA法测定小鼠体液中狂犬病病毒抗体IgG(RV-IgG)浓度。结果与Y1组及Y2组比较,Y3组初次免疫后3 d小鼠脾细胞刺激指数(stimulatingindex,SI)值明显升高(P <0. 01);初次免疫后7、14、21、28 d,CD4^+/CD8^+值明显升高(P <0. 05),初次免疫后7、14、21 d,RV-IgG浓度明显升高(P <0. 05)。结论狂犬病疫苗脂质体冻干粉在小鼠体内具有良好的免疫原性,脂质体能提高疫苗免疫原性,延长疫苗的免疫时间,有望开发成为新一代广泛应用的疫苗佐剂。     

不同来源苦荞中芦丁和槲皮素的含量测定

建立了一种苦荞中芦丁和槲皮素含量的测定方法.以超声法进行提取,并分别以芦丁和槲皮素为标品,采用岛津ODS柱(4.6mm×150mm,5μm),以乙腈-水(含2%的冰醋酸)为流动相,梯度洗脱,流速:1 mL·min-1,检测波长:360nm.芦丁进样量在0.023～1.150μg范围内(r=0.9996),槲皮素进样量在...     

高效液相色谱法同时测定咖啡中6种绿原酸

目的建立咖啡中同时测定5-咖啡酰奎宁酸、3-咖啡酰奎宁酸、4-咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸、4,5-二咖啡酰奎宁酸、3,5-二咖啡酰奎宁酸6种绿原酸的高效液相色谱(high performance liquid chromatography,HPLC)检测方法,以及测定产自云南普洱的铁皮卡品种咖啡在不同烘焙风味下的绿原酸含量规律。方法样品经纯水超声提取2次,过滤后供HPLC测定,采用甲醇(A)和0.1%磷酸(B)作为流动相进行梯度洗脱采用C18柱进行分离,光电二极管阵列(photo-diode array,PDA)检测器最终检测。结果本方法在1 h内完成了6种绿原酸的分离分析。6种绿原酸在0.10～100.0 mg/L范围内具有良好的线性,相关系数(r)≥0.999,检出限为0.05～0.10 mg/kg,定量限为0.18～0.29 mg/kg。在2000、5000、10000 mg/kg添加水平的加标回收率为96.3%～104.8%,相对标准偏差(relative standard deviation,RSD)为0.58%～2.10%之间(n=6)。在生咖啡豆中总绿原酸含量最高,意式烘焙(23 min,250℃)最低,5-咖啡酰奎宁酸、4-咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸这3种绿原酸在生咖啡豆到极轻度烘焙(12 min,230℃)这个阶段,含量逐渐上升并达到含量最高,由极轻度烘焙(12 min,230℃)到意式烘焙(23min,250℃)含量逐渐降低,而3-咖啡酰奎宁酸、3,4-二咖啡酰奎宁酸和3,5-二咖啡酰奎宁酸3种绿原酸从生咖啡豆至意式烘焙(23 min,250℃)都呈下降趋势。结论该方法快速、准确、灵敏,适合测定绿原酸含量。     

十二烷基三甲基氯化铵从碱性氰化液中固相萃取金的研究

把一直应用于萃取有机物的固相萃取技术(SPE)用于萃取无机物金,研究了SPE与表面活性剂十二烷基三甲基氯化铵(DTMAC)液-液萃取(LLE)金两种技术,探索出一种从低浓度碱性氰化液中固相萃取金的最佳萃取条件。最佳萃取条件为:被萃液中DTMAC∶Au的摩尔比为1.5∶1.0;小柱须经过一定量0.01015 mol·L-1的DTMAC处理;萃取液pH为10.5;过柱流速为15 ml·min-1;C18,C8,C4,C24种填料柱对金的萃取容量都为4 mg,推荐使用C18小柱;乙醇为洗脱剂;洗脱流速为5 ml·min-1;萃取机制为:在碱性条件下DTMAC与Au(CN)-2能反应生成离子缔合物,该离子缔合物能被固相柱萃取富集;富集倍数高达500倍;选择性高;一次萃取率可超过98%,固相萃取柱不容易被破坏可重复使用。     

RP-HPLP法测定果脯中甘草苷和甘草酸的含量

目的:建立用RP-HPLC法同时测定果脯中甘草苷和甘草酸含量的方法。方法:采用LiChrospher RP- C18（250mm×4．6mm,i．d．5μm）色谱柱,以乙腈-水（1．0%甲酸）为流动相,梯度洗脱,双波长检测。结果:甘草苷在0．21～6．30μg范围内呈良好的线性关系,回归方程为Y=2334．3161X+25．2431（r=0．9999）,平均回收率为98．6%,RSD为1．36%（n=9）,重复性RSD为0．78%;甘草酸在0．25～7．50μg范围内呈良好的线性关系,回归方程为Y=907．7837X+0．8969（r=0．9999）,平均回收率为100．2%,RSD为1．48%（n=9）,重复性RSD为1．25%。结论:该方法简便迅速,结果可靠,分离效果好,可作为控制食品中甘草限量的方法。     

Biophenolic profile in olives by nuclear magnetic resonance

The molecular composition of biophenols in olive fruit was investigated in order to experiment with novel procedures for the determination of these microcomponents in fresh and processed table olives. The presence of biophenols in table olives, with recognized antioxidant activity, can be strictly linked to the texture and the organoleptic characteristics of the food product, giving a functional value to this Mediterranean food. Olives from Spain (“Hojiblanca” cv.), Portugal (“Douro” cv.), Greece (“Conservolia” and “Thasos”cv.) and Italy (“Taggiasca” and “Cassanese” cv.) were examined, because the experimental data, checked by high-performance liquid chromatography (HPLC), show molecular composition differences in the tested samples related to the geographic area of analyzed olive fruit cultivars [Bianco, A., & Uccella, N. (2000). Biophenolic components of olives. Food Research International, 33, 475–485]. Three different protocols were utilized: the first one allows the determination of the biophenolic content present as free and esterified compounds; the second affords the total biophenolic content; the third indicates the biophenols present as glycosides. The biophenolic content, which was previously determined by classic HPLC methods (Bianco & Uccella, 2000), was checked by a simple ¹H-NMR experiment. The comparison between the data obtained from ¹H-NMR with those measured by HPLC, indicates a good agreement and suggests the possibility of employing ¹H-NMR for the rapid determination of biophenolic content in olives and also in other foods.     

Green and ecological interior design based on network processor and embedded system

With the growing demand for interior design solutions that are friendly to the green environment, there is a growing awareness of interior designers around the world to the sustainability strategy. Most of the traditional interior design and construction process consumes many natural resources in the manufacturing and installation process. This will harm the environment. Interior designers adopted a sustainable design technology, the opportunity to improve the healthy indoor air quality. To achieve sustainable solutions, materials, construction methods, furniture by selecting the lighting design options. Despite much sustainable design research, discussion of the interior design of sustainability criteria there little. Essential for sustainable standard interior design solutions to support the responsible interior designer. Therefore, this study aims to propose a set of criteria for selecting based on sustainable indoor network processors and embedded system design solutions to ensure a sustainable and consistent product mix of 3DWeb representation of interior design solutions thing. Finally, to enhance its functionality, come to an end because of the importance of effective standards.     

Influence of dentin biomodification with epigallocatechin-3-gallate on the bond strength of self-etch adhesive: Twelve-month results

To evaluate the effectiveness of dentin biomodification with epigallocatechin-3-gallate (EGCG) on the resin-dentin bonds over time. Twenty seven extracted human third molars were prepared to expose a flat dentin surface and divided into 3 groups (n=9). Dentin surfaces were dried and treated with 20 µL aliquots of either distilled water (control); 2% chlorhexidine digluconate solution (CHX) or 0.1% EGCG aqueous solution. Solutions were rubbed for 60 s followed by bonding with Adper Easy One, and 5-mm-thick resin crown build-up. Bonded teeth were stored in distilled water for 24 h and then longitudinally sectioned to obtain bonded sticks. One-third of the specimens were immediately subjected to a microtensile bond strength test in tension at 0.5 mm/min, while the remaining specimens were tested after six and twelve months of storage in distilled water at 37 °C. Data were analyzed with Two-way ANOVA and Holm–Sidak method. After 24 h of storage, mean bond strength values were not significantly different among all groups (p>0.05). Bond strengths of EGCG and CHX remained stable after 6 and 12 months. (p>0.05). To conclude, pretreatment with EGCG or CHX preserved the bonding of Adper Easy One to dentin after six and twelve months of storage.     

Fast condensation of cyclohexanone with 2-aminobenzonitrile at room temperature catalysed by an N-heterocyclic carbene

A fast condensation of cyclohexanone with 2-aminobenzonitrile catalysed by N-heterocyclic carbene 1,3-dipropylimidazole-2-ylidene (NHC-PPIm) at room temperature was discovered. NHC-PPIm was prepared via concentration of a 1,3-dipropylimidazolium hydroxide aqueous solution and showed excellent catalytic activity in the condensation reaction for the synthesis of quinazolinone. A possible catalytic mechanism was proposed based on the umpolung of cyclohexanone.