Monitoring and dietary exposure assessment of pesticide residues in cowpea (Vigna unguiculata L. Walp) in Hainan,China

To monitor pesticide residues in cowpea and assess the potential public health risk, 433 fresh cowpea samples from Hainan province (2012 and 2013) were collected and the presence of 20 organophosphoate, carbamate and pyrethroid was determined. A screening analysis consisting of a dietary exposure assessment of pesticide residue was performed using a deterministic approach-point estimate of exposure. The results indicated the most important residues were triazophos, carbofuran, isocarbophos, phoxim and omethoate. The estimated daily intakes (EDIs) of those pesticides were 72.89%, 27.45%, 12.35%, 12.19% and 9.15% of Acceptable Daily Intake (ADI) respectively. Then a probabilistic approach applied on the residues of concern demonstrated that exposure from triazophos exceeded the ADI at P99.9. A relative potency factors method was employed to ascertain that exposure to triazophos is a cause for concern.     

贝母属药用植物叶绿体基因组单核苷酸多态性位点生物信息学分析

目的分析贝母属药用植物叶绿体基因组单核苷酸多态性(single nucleotide polymorphism,SNP)位点的分布特点、鉴别能力和应用价值,为中药材贝母精准鉴定方法的建立提供技术支撑。方法应用多重序列比对、SNP筛选、酶切位点分析等生物信息学分析手段,对贝母属6种药用植物的15条叶绿体基因组序列进行分析。结果贝母属叶绿体基因组DNA同源性达98.38%,通过分析共发现SNP位点4058个,其中川贝母类鉴别候选位点71个,瓦布贝母鉴别候选位点个25个,太白贝母鉴别候选位点个120个,浙贝母鉴别候选位点个79个,湖北贝母鉴别候选位点个61个,平贝母鉴别候选位点794个。结论叶绿体基因组SNP分子标记因其密度高、鉴别力强和便于分析的特点,具有较大的应用价值。