排序方式: 共有3条查询结果,搜索用时 62 毫秒
1
1.
2.
Steffen Reinbothe Claudia Rossig John Gray Sachin Rustgi Diter von Wettstein Christiane Reinbothe Joachim Rassow 《International journal of molecular sciences》2021,22(8)
Aminoacyl-tRNA synthetases (AaRS) charge tRNAs with amino acids for protein translation. In plants, cytoplasmic, mitochondrial, and chloroplast AaRS exist that are all coded for by nuclear genes and must be imported from the cytosol. In addition, only a few of the mitochondrial tRNAs needed for translation are encoded in mitochondrial DNA. Despite considerable progress made over the last few years, still little is known how the bulk of cytosolic AaRS and respective tRNAs are transported into mitochondria. Here, we report the identification of a protein complex that ties AaRS and tRNA import into the mitochondria of Arabidopsis thaliana. Using leucyl-tRNA synthetase 2 (LeuRS2) as a model for a mitochondrial signal peptide (MSP)-less precursor, a ≈30 kDa protein was identified that interacts with LeuRS2 during import. The protein identified is identical with a previously characterized mitochondrial protein designated HP30-2 (encoded by At3g49560) that contains a sterile alpha motif (SAM) similar to that found in RNA binding proteins. HP30-2 is part of a larger protein complex that contains with TIM22, TIM8, TIM9 and TIM10 four previously identified components of the translocase for MSP-less precursors. Lack of HP30-2 perturbed mitochondrial biogenesis and function and caused seedling lethality during greening, suggesting an essential role of HP30-2 in planta. 相似文献
3.
Teresa Murino Mario Di Nardo Daniele Pollastro Nicole Berx Angela Di Francia Wilm Decré Johan Philips Liliane Pintelon 《Quality and Reliability Engineering International》2023,39(3):706-731
Collaborative robots are an emerging technology falling within the scope of Industry 4.0 and based on the concept of Human-Robot Collaboration (HRC). Unlike traditional industrial robots, collaborative robots are used in shared workspaces with no safety fences. Hence, prospective hazardous contacts need to be avoided or mitigated through a risk assessment. The normative standards such as ISO TS 15066 suggest a list of common hazards, but do not guide the robot system user through the risk assessment process. To address this shortcoming, this paper proposes a practical eight steps risk assessment approach, resulting in a risk priority list. In order to provide an accurate, practical, quantitative and supportive tool for HRC environments, the Failure Mode and Effects Analysis (FMEA) and the Proportional Risk Assessment technique (PRAT) techniques are proposed for risk assessment. The two techniques mentioned above are combined in the suggested new methodology, highlighting both their benefits and disadvantages. The proposed methodology is applied with positive results to a collaborative brick-lifter case study. 相似文献
1