广西产迷迭香挥发油化学成分的分析

水蒸气蒸馏法提取广西百色地区迷迭香茎、叶中的挥发油,利用GC-MS联用仪进行了分析。共分离出36个峰,鉴定了其中31个化学成分,占总含量的97.08%。其主要成分为1,8-桉叶素、α-蒎烯、樟脑、莰烯、α-松油醇、乙酸龙脑酯及龙脑等。     

Effect of relative humidity and light conditions on the oxidative stability of sunflower oil blends stabilised with synthetic and natural antioxidants

The effect of relative humidity (RH) and light conditions on the oxidative stability of sunflower oil (SO) stabilised with oleoresin rosemary + ascorbyl palmitate (SOR), sage + ascorbyl palmitate (SOS) and tertiary butyl hydroquinone (SOT) was investigated. The SO without additive (SOC) served as positive control. Oil samples were subjected to 3 RH (29%, 52% and 75%) and two light conditions (dark, D and fluorescent light (600 lx), L) at 60 °C for 7 days. The oxidative stability was monitored by peroxide value (PV), conjugated diene value (CDV), free fatty acids (FFAs) and Rancimat induction period (IP). A direct relationship was noted between RH and increase in PV, CDV and FFA levels irrespective of light conditions. The detrimental effect of light on the oxidative stability was prominently noted at higher RH (75%) which followed an order: SOC > SOT > SOR > SOS. The samples stored under lower RH (29% and 52%), and dark conditions were better stabilised than light‐exposed counterparts.     

Rosmarinic Acid,as an NHE1 Activator,Decreases Skin Surface pH and Improves the Skin Barrier Function

Stratum corneum (SC) pH regulates skin barrier functions and elevated SC pH is an important factor in various inflammatory skin diseases. Acidic topical formulas have emerged as treatments for impaired skin barriers. Sodium proton exchanger 1 (NHE1) is an important factor in SC acidification. We investigated whether topical applications containing an NHE1 activator could improve skin barrier functions. We screened plant extracts to identify NHE1 activators in vitro and found Melissa officinalis leaf extract. Rosmarinic acid, a component of Melissa officinalis leaf extract, significantly increased NHE1 mRNA expression levels and NHE1 production. Immunofluorescence staining of NHE1 in 3D-cultured skin revealed greater upregulation of NHE1 expression by NHE1 activator cream, compared to vehicle cream. Epidermal lipid analysis revealed that the ceramide level was significantly higher upon application of the NHE1 activator cream on 3D-cultured skin, compared to application of a vehicle cream. In a clinical study of 50–60-year-old adult females (n = 21), application of the NHE1 activator-containing cream significantly improved skin barrier functions by reducing skin surface pH and transepidermal water loss and increasing skin hydration, compared to patients who applied vehicle cream and those receiving no treatment. Thus, creams containing NHE1 activators, such as rosmarinic acid, could help maintain or recover skin barrier functions.     

生姜黄酮的提取及其抗氧化活性的研究

研究了从生姜中提取黄酮的工艺，结果表明：以80%的乙醇水溶液为溶剂，固液比为1:2，在80℃下回流3h，提取率最高。并在不同条件下探索了提取液的稳定性和抗氧化活性。     

芦笋中甾体皂苷结构和功能特性的研究进展

对其药用功效有显著贡献的甾体皂苷类物质的提取方法、分离纯化方法、结构鉴定方法以及国内外对芦笋中甾体皂苷的结构鉴定方面的研究报道进行综述。重点归纳已报道的27 种芦笋甾体皂苷的结构和功能特性,并分析和讨论今后的主要研究方向。     

五种中草药水溶性多糖对α-葡萄糖苷酶活性的影响

从山茱萸、知母、甘草、地榆及诃子中提取水溶性多糖,并通过酶抑制动力学反应考察其对α-葡萄糖苷酶活性的影响。酶动力学反应结果表明:地榆多糖对α-葡萄糖苷酶有激活作用;知母多糖对α-葡萄糖苷酶的活性随时间的变化先激活后抑制;山茱萸多糖、甘草多糖及诃子多糖均对α-葡萄糖苷酶有抑制活性,其中抑制活性最好的是甘草多糖,其抑制率为72.5%;其各自的影响机理还有待进一步研究。     

鼠尾草提取物多酚含量与抗氧化活性的研究

研究从不同的角度来评价鼠尾草提取物的抗氧化活性。采用50%丙酮和70%乙醇两种溶剂对鼠尾草进行提取,用Folin-Ciocalteu法测定提取物中的多酚含量,并用四种方法,即清除DPPH（1,1-二苯基-2-三硝基苯肼）自由基法、螯合Fe2＋法、还原力法和磷钼络合物法测定提取物的抗氧化活性。研究结果表明,50%丙酮提取物中的多酚含量显著高于70%乙醇提取物。鼠尾草提取物具有良好的自由基清除能力、螯合Fe2＋能力、还原能力和总抗氧化能力。鼠尾草50%丙酮提取物的抗氧化活性总体上比70%乙醇提取物高（还原力除外）。     

毛细管区带电泳法测定霍香正气胶囊中和厚朴酚的含量

目的:建立霍香正气胶囊中和厚朴酚含量测定的毛细管区带电泳(CZE)法.方法:采用甲醇溶解样品超声制备供试液;运行缓冲液为40mmol/L硼砂溶液,检测波长254nm.结果:和厚朴酚线性范围0.013-0.20mg/mL,r=0.9917(n=5),厚霍香正气胶囊中和厚朴酚的含量为0.1479mg/粒.结论:此方法快速简便,可用于评价霍香正气胶囊的质量.     

山茱萸有效成分和药理作用的研究进展及其在食品工业中的应用

山茱萸是一种重要的野生植物资源，也是我国传统的中药材。山茱萸中含有多种具有药用价值的物质，从果肉中分离得到的主要成分可分为有机酸及其酯类，五环三萜及其酯类，环烯醚萜类，鞣质类，维生素，氨基酸和矿物质等。山茱萸的药理作用主要包括免疫作用，降血糖作用，抗炎作用和抗休克，强心作用等。目前国内和国外山茱萸产品并不多，大体上可以分为以下四类：补酒，口服液，饮料和果脯蜜饯，果酱，罐头等。     

Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus Fomes sclerodermeus

Fomes sclerodermeus was grown on semi‐defined media based on yeast extract, peptone and glucose (YPG). The fungus produced a minimum basal level of laccase activity irrespective of culture medium. The highest laccase production (20 U cm^?3) was obtained in cultures supplemented with CuSO₄. Manganese peroxidase (MnP) could only be detected when MnSO₄ was added to the medium. None of the aromatic compounds tested stimulated further laccase or MnP production. Laccase and MnP stimulated by Cu²⁺ or Mn²⁺ respectively were purified. Two different laccase isoenzymes with the same molecular mass (67 kDa) and N‐linked carbohydrate content (3%) and a slight difference in their pI values (3.41 and 3.48) were characterized. In addition, two different MnP isoenzymes with the same molecular mass (47 kDa) and N‐linked carbohydrate content (4%) and different pI values (3.35 and 3.45) were characterized. Both enzymes showed good stability at 25 °C and over a wide range of pH. Both laccases oxidize ABTS (2,2′‐azino‐bis(3‐ethylbenzthiazoline‐6‐sulfonic acid) more efficiently than 2,6‐dimethoxyphenol (DMP) with similar efficiency values (K_cat/K_m) while the MnP I, the major peroxidase isoenzyme in the studied conditions, oxidizes the Mn²⁺ and Mn‐mediated activity on DMP more efficiently than MnP II. Copyright © 2006 Society of Chemical Industry