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1.
ABSTRACT: This study determined the efficacy and safety of ozonation in degrading aflatoxin in corn. Ozonation (10 to 12 wt%) reduced aflatoxin levels by 92% and no reversion to the parent compound was observed. Ozonation had minimal effect on fatty acids of uncontaminated corn, but had significant effect on fatty acids of contaminated corn. Crude extracts showed no mutagenic potential in the Ames assay using TA98 and TA100. Clean-up using hexane increased their mutagenic potentials. Clean-up using Mycosep columns increased the mutagenic potentials 18 to 617%. Hexane extracts from ozone-treated contaminated corn had lower inhibitory effect. This suggested that a fat-soluble mutagen is being formed or natural inhibitors of mutagenicity are being destroyed. 相似文献
2.
Kerstin Skog Åsa Eneroth & Maria Svanberg 《International Journal of Food Science & Technology》2003,38(3):313-323
Hamburgers and chicken fillets were cooked in convection ovens, deep‐fried or contact fried and analysed for mutagenic activity using the Ames test. For the three different convection ovens, the cooking parameters studied included the presence of steam, air velocity, air temperature and holding time. For deep‐frying and contact frying, the cooking parameters included cooking temperature and cooking time. In cooked hamburgers, mutagenic activity was only detected in those that had been deep‐fried. In chicken fillets, mutagenic activity was detected in samples prepared with all cooking methods, being highest in the deep‐fried samples. Factorial analysis indicated that heat transfer was the most important factor affecting mutagenic activity. High temperature and high air velocity in the convection ovens enhanced mutagenic activity. The presence of steam reduced the mutagenic activity, except when high temperature was used in combination with high air velocity. In chicken fillets, high mutagenic activity correlated to high weight loss during cooking. Pan‐fried chicken fillets were analysed using high performance liquid chromatography (HPLC) and the mutagenic/carcinogenic heterocyclic amines 2‐amino‐3,8‐dimethylimidazo[4,5‐f ]‐quinoxaline (MeIQx), 2‐amino‐1‐methyl‐6‐phenylimidazo‐[4,5‐b]‐pyridine (PhIP) and the co‐mutagenic heterocyclic amine Norharman (9H‐pyrido[3,4‐b]‐indole) were identified. The HPLC fractions were tested for mutagenic activity and, apart from the mutagenic fractions corresponding to MeIQx and PhIP, several mutagenic fractions were detected that did not correspond to known heterocyclic amines. 相似文献
3.
Ames试验氯化致突变前体物在净水工艺中的去除研究 总被引:2,自引:1,他引:1
采用水样比活性作为评价指标,从Ames试验结果和致突变前体物性质两方面,探讨了不同单元净水工艺对Ames试验氯化致突变前体物的去除能力,从而为净水工艺的选择提供参考依据 相似文献
4.
水蒸气蒸馏法提取广西百色地区迷迭香茎、叶中的挥发油,利用GC-MS联用仪进行了分析。共分离出36个峰,鉴定了其中31个化学成分,占总含量的97.08%。其主要成分为1,8-桉叶素、α-蒎烯、樟脑、莰烯、α-松油醇、乙酸龙脑酯及龙脑等。 相似文献
5.
白蚁提取液急性毒性及致突变性研究 总被引:1,自引:0,他引:1
本文采用大,小鼠急性经口毒性试验、Ames试验、小鼠骨髓细胞微核试验及小鼠精子畸变试验对白蚁提取液的毒理学安全性进行了研究,结果表明,白蚁提取液大,小鼠急性经口LD50均大于10g/kg体重,属“实际无毒”:体内(包括体细胞和生殖细胞)、体外致突变性试验系统均未检出其致突性。 相似文献
6.
Effect of relative humidity and light conditions on the oxidative stability of sunflower oil blends stabilised with synthetic and natural antioxidants
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Rohit Upadhyay Hari Niwas Mishra 《International Journal of Food Science & Technology》2016,51(2):293-299
The effect of relative humidity (RH) and light conditions on the oxidative stability of sunflower oil (SO) stabilised with oleoresin rosemary + ascorbyl palmitate (SOR), sage + ascorbyl palmitate (SOS) and tertiary butyl hydroquinone (SOT) was investigated. The SO without additive (SOC) served as positive control. Oil samples were subjected to 3 RH (29%, 52% and 75%) and two light conditions (dark, D and fluorescent light (600 lx), L) at 60 °C for 7 days. The oxidative stability was monitored by peroxide value (PV), conjugated diene value (CDV), free fatty acids (FFAs) and Rancimat induction period (IP). A direct relationship was noted between RH and increase in PV, CDV and FFA levels irrespective of light conditions. The detrimental effect of light on the oxidative stability was prominently noted at higher RH (75%) which followed an order: SOC > SOT > SOR > SOS. The samples stored under lower RH (29% and 52%), and dark conditions were better stabilised than light‐exposed counterparts. 相似文献
7.
Seung-Won Jung Gi Hyun Park Eunjung Kim Kang Min Yoo Hea Won Kim Jin Soo Lee Min Youl Chang Kyong-Oh Shin Kyungho Park Eung Ho Choi 《International journal of molecular sciences》2022,23(7)
Stratum corneum (SC) pH regulates skin barrier functions and elevated SC pH is an important factor in various inflammatory skin diseases. Acidic topical formulas have emerged as treatments for impaired skin barriers. Sodium proton exchanger 1 (NHE1) is an important factor in SC acidification. We investigated whether topical applications containing an NHE1 activator could improve skin barrier functions. We screened plant extracts to identify NHE1 activators in vitro and found Melissa officinalis leaf extract. Rosmarinic acid, a component of Melissa officinalis leaf extract, significantly increased NHE1 mRNA expression levels and NHE1 production. Immunofluorescence staining of NHE1 in 3D-cultured skin revealed greater upregulation of NHE1 expression by NHE1 activator cream, compared to vehicle cream. Epidermal lipid analysis revealed that the ceramide level was significantly higher upon application of the NHE1 activator cream on 3D-cultured skin, compared to application of a vehicle cream. In a clinical study of 50–60-year-old adult females (n = 21), application of the NHE1 activator-containing cream significantly improved skin barrier functions by reducing skin surface pH and transepidermal water loss and increasing skin hydration, compared to patients who applied vehicle cream and those receiving no treatment. Thus, creams containing NHE1 activators, such as rosmarinic acid, could help maintain or recover skin barrier functions. 相似文献
8.
吲熟酯浓度为 1、1 0、1 0 0、50 0、和 1 0 0 0 μg/皿 ,在加S9或不加S9的试验条件下 ,对TA97、TA98、TA10 0 、TA10 2 四个菌株进行Ames试验 ,结果表明 ,各浓度组的MR值均小于 2 ;以剂量为 2 0、1 0 0、2 0 0和 4 0 0mg/kg体重 ,进行小鼠微核试验 ,观察各剂量组小鼠骨髓嗜多染红细胞微核率结果与对照组比较均未见引起显著性增加 (p >0 .0 5) ;以剂量为 4 0 0、80 0、1 0 0 0mg/kg体重进行小鼠睾丸精母细胞染色体畸变试验 ,各剂量组的染色体畸变细胞率分别为0 .80 %、0 .80 %和 1 .2 % ,与对照组比较均无显著性差异 (p >0 .0 5)。三项试验的结果均表明吲熟酯未显有致突变作用。 相似文献
9.
生姜黄酮的提取及其抗氧化活性的研究 总被引:40,自引:6,他引:40
研究了从生姜中提取黄酮的工艺,结果表明:以80%的乙醇水溶液为溶剂,固液比为1:2,在80℃下回流3h,提取率最高。并在不同条件下探索了提取液的稳定性和抗氧化活性。 相似文献
10.