北欧国家方法验证委员会替代专有化学方法验证指南

本文全文译自北欧国家方法验证委员会（NordVal）2010年5月26日批准的“替代专有化学方法验证指南”（NordVal第2号规程）。NordVal由北欧部长理事会下属的北欧食品议题高级官员委员会创建,并将评估替代微生物方法和替代化学方法的性能和适用范围作为其工作目标,涵盖北欧国家范围内的食品、水、饲料、动物粪便和食品环境样本的分析。本文可作为我国食品分析专业人员研发和验证化学检测方法或与北欧检测机构交流的参考。     

Validation of a Diagnostic PCR Method for Routine Analysis of Salmonella spp. in Animal Feed Samples

As a part of a validation study, a comparative study of a PCR method and the standard culture-based method NMKL-71, for detection of Salmonella, was performed according to the validation protocol from the Nordic validation organ for validation of alternative microbiological methods (NordVal) on 250 artificially or naturally contaminated animal feed samples. The PCR method is based on culture enrichment in buffered peptone water followed by PCR using the DNA polymerase Tth and an internal amplification control. No significant difference was found between the two methods. The relative accuracy, relative sensitivity and relative specificity were found to be 96.0, 97.3, and 98.8%, respectively. PCR inhibition was observed for rape seed samples. For the acidified feed samples, more Salmonella-positive samples were found with the PCR method compared to the NMKL method. This study focuses on the growing demand for validated diagnostic PCR methods for routine analysis of animal feed and food samples to assure safety in the food production chain.