减肥类中成药、保健食品、食品中非法添加酚酞、西布曲明等化学成分的快筛检测方法研究

目的建立快筛检测减肥类中成药、保健食品和食品中非法添加酚酞、西布曲明等化学成分的方法。方法用快筛试管法和薄层色谱法检测产品所含的酚酞、西布曲明等化学成分,并通过HPLC-DAD/HPLC-MS确认。结果 103批次产品中42批次检出酚酞,61批次检出西布曲明,其他相关化学成分目前均未检出。结论此方法专属性强、灵敏度高、重现性好,是一种简便快捷、经济实用、准确判断减肥类产品是否非法添加酚酞、西布曲明等化学成分的快筛检测方法,适用于快速鉴别筛查基层大批量产品。     

不同茶多糖对3T3-L1前脂肪细胞分化的抑制作用比较

本实验利用小鼠3T3-L1前脂肪细胞对绿茶多糖（green tea polysaccharides,GTP）、红茶多糖（black tea polysaccharides,BTP）和乌龙茶多糖（oolong tea polysaccharides,OTP）的减肥作用进行评价。使用气相色谱对GTP、BTP、OTP的单糖组成进行分析。采用噻唑蓝染色法测定3?种茶多糖（tea polysaccharides,TPs）对3T3-L1前脂肪细胞增殖活力的影响。使用流式细胞仪检测3?种TPs对3T3-L1前脂肪细胞细胞周期的影响。采用传统“鸡尾酒”法诱导3T3-L1细胞分化成脂后,测吸光度并计算其分化率。采用甘油磷酸氧化酶-过氧化物酶（glycerol phosphate oxidase-peroxidase,GPO-PAP）法测定细胞中甘油三酯（triglyceride,TG）含量的变化。采用实时荧光定量聚合酶链式反应（real-time polymerase chain reaction,RT-PCR）技术测定与脂质代谢相关基因的表达量。结果显示3?种TPs均能显著抑制3T3-L1前脂肪细胞的增殖与分化（P＜0.05）。添加100?μg/mL的TPs能使细胞分化率显著下降至（62.00±6.61）%（GTP）、（82.95±4.25）%（BTP）、（97.24±5.80）%（OTP）。另外,在细胞培养至第5天检测表明,这3 种TPs显著促进G0/G1期细胞数量的累积,细胞比例分别为（68.52±2.28）%（GTP）、（67.11±1.68）%（BTP）、（59.69±1.35）%（OTP）。RT-PCR分析结果显示TPs可以调控相关脂肪细胞因子的表达。在本研究中,在3T3-L1前脂肪细胞中GTP的减肥作用强于BTP和OTP。TPs的加入上调脂联素的表达从而激活腺苷酸活化蛋白激酶信号通路调控相关脂肪细胞因子的表达,并最终抑制TG的合成与3T3-L1前脂肪细胞的分化。     

Screening of crude plant extracts with anti-obesity activity

Obesity is a global health problem. It is also known to be a risk factor for the development of metabolic disorders, type 2 diabetes, systemic hypertension, cardiovascular disease, dyslipidemia, and atherosclerosis. In this study, we screened crude extracts from 400 plants to test their anti-obesity activity using porcine pancreatic lipase assay (PPL; triacylglycerol lipase, EC 3.1.1.3) in vitro activity. Among the 400 plants species examined, 44 extracts from plants, showed high anti-lipase activity using 2,4-dinitrophenylbutyrate as a substrate in porcine pancreatic lipase assay. Furthermore, 44 plant extracts were investigated for their inhibition of lipid accumulation in 3T3-L1 cells. Among these 44 extracts examined, crude extracts from 4 natural plant species were active. Salicis Radicis Cortex had the highest fat inhibitory activity, whereas Rubi Fructus, Corni Fructus, and Geranium nepalense exhibited fat inhibitory capacity higher than 30% at 100 μg/mL in 3T3-L1 adipocytes, suggesting anti-obesity activity. These results suggest that four potent plant extracts might be of therapeutic interest with respect to the treatment of obesity.     

Novel semicarbazide-sensitive amine oxidase inhibitor screening from anti-obesity drugs using HPLC-MS based technique

Semicarbazide-sensitive amine oxidase ( SSAO) has been considered to be associated with the metabolism of glucose and fat,and elevated SSAO activity was observed in obese patients. In the present study,an in vitro SSAO activity-based method was developed to screen inhibitors from 1 5 anti-obesity drugs. Among the fifteen anti-obesity drugs,four drugs including caffeine,fenfluramine, bumetanide and amfebutamone inhibited SSAO activity,and caffeine was the most effective one. When the concentration of caffeine was 1 . 4 mmol / L,the inhibition ratio was 3 1 . 9 % and 1 8 . 8 % in rabbit serum and rat adipose tissue,respectively. Inhibition of SSAO activity by caffeine was also confirmed in the in vivo study,showing the inhibition ratio of 1 5 . 6 % on serum SSAO. Caffeine provides a natural source of inhibition of SSAO activity and may be a promising inhibitor for the study of SSAO.     

红小豆醇提物对摄食高脂料小鼠的降脂减肥作用

目的:研究红小豆醇提物对摄食高脂料的昆明白雌、雄小鼠的降脂减肥作用.方法:以饲喂基础料、高脂科的小鼠为对照,以红小豆醇提物按低、中、高(即50、100、200 mg/kg小鼠体重)3个剂量分别强饲给摄食高脂料的小鼠,即试验组.试验期间小鼠自由采食、饮水,共饲养8周,定期检测小鼠的日摄食量及体重/周.8周后,检测小鼠李氏指数、肝脏质量以及不同部位脂肪质量,小鼠血液中葡萄糖、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白( HDL-C)、胆固醇/高密度脂蛋白(TC/HDL-C)水平.结果:红小豆醇提物各组别能明显降低雌、雄小鼠体重及生殖器官周围的脂肪组织质量(p＜0.01,0.05).对雌性小鼠李氏指数、肝脏质量有明显的降低作用(p＜0.05,0.01),对雄性小鼠则无显著差异(p＞0.05).红小豆醇提物能促进雌、雄小鼠的采食.血液指标方面,红小豆醇提物仅对雌性小鼠有一定的降脂效果,其中高剂量最佳.结论:红小豆醇提物对摄食高脂料小鼠有一定的减肥作用,其中对雌性小鼠降脂减肥效果优于雄性小鼠.     

岩藻黄素抗肥胖和抗糖尿病活性研究进展

目前肥胖和Ⅱ型糖尿病已迅速成为世界范围内的流行病。一种在褐藻中发现的典型类胡萝卜素-岩藻黄素,具有潜在的抗肥胖和抗糖尿病活性,可能对治疗肥胖以及糖尿病有显著疗效。岩藻黄素含有一个丙二烯键以及两个羟基的多烯发色团尾端,这种独特的化学结构是其具有重要生物活性的关键因素。岩藻黄素可以诱导线粒体解偶联蛋白1(UCP1)在白色脂肪组织(White Adipose Tissue,WAT)的表达从而增加产热,还可以通过调节WAT中的细胞因子分泌来改善胰岛素抵抗并降低血糖水平。本文通过查阅大量相关文献,对岩藻黄素抗肥胖以及抗糖尿病作用进行全面综述。     

复合减肥茶及其减肥机理的研究

研究了乌龙茶以及山楂、荷叶等常见食用中药对大鼠离体激素敏感性脂肪酶和胰脂酶活性的影响, 据此确定复合减肥茶的配方,并通过动物试验检验了其减肥功效。试验结果表明, 该减肥茶能极显著地提高激素敏感性脂肪酶活性(p<0.01),增加离体脂肪释放FFA的百分率达112.9%;能有效地抑制营养肥胖型大鼠体重的增长和减少其体内脂肪含量(p<0.01);降血脂作用显著(p<0.05),减肥效果良好。