Annual and regional variations of aflatoxin B1 levels seen in grains and feed coming from Croatian dairy farms over a 5-year period

The aim of the study was to investigate annual and regional differences in the level of aflatoxin B₁ (AFB₁) in grains and dairy cattle feed. Maize (n = 972), wheat (n = 201), barley (n = 147), oat (n = 136), grain mixtures (n = 168), and dairy cattle feed (n = 325) were sampled from 2009 to 2013 on different farms and in different farm factories situated in four Croatian regions. The samples were analysed for AFB₁ using the validated ELISA immunoassay. AFB₁ was determined in 16.4% of all investigated samples, among which maize was proven to be the most contaminated, with 21.7% of the samples recovered during 2013 harbouring AFB₁ in concentrations over the permissible ones. Levels higher than permitted were observed in 17.9% and 12.3% of grain mixtures and dairy cattle feed, respectively, whereas concentrations of AFB₁ determined in other crops throughout the investigated period met the stipulated requirements. The results revealed the AFB₁ occurrence to be significantly (p < 0.05) dependent on the cultivation region, with the highest levels generally found in maize harvested in 2013 and consequently in grain mixtures and cattle feed that can most likely be associated with climatic conditions as the most critical factor for mould formation, and thus also AFB₁ production.     

基于蛋白质组学技术研究秦川牛肉宰后贮藏过程中肌红蛋白含量及其衍生物转化

本实验采用4D-非标记蛋白质组学技术研究秦川牛肉贮藏过程中（0～8 d）肌红蛋白含量及其衍生物的转化情况，阐释冷却秦川牛肉中肌红蛋白含量及其衍生物转化的分子机制。结果表明：贮藏过程中，肌红蛋白表达量在宰后0～4 d上升、4～8 d下降，利用非标记蛋白质组学技术鉴定出与肌红蛋白及其衍生物相关的差异蛋白14 种，具体包括代谢酶、氧化还原酶、过氧化物酶、伴侣蛋白4 类，这4 类蛋白的表达共同调控贮藏过程中肌红蛋白含量的变化及其3 种衍生物之间的转化，具体表现为贮藏过程中肌红蛋白表达量整体呈下降趋势，氧合肌红蛋白相对含量持续下降，脱氧肌红蛋白、高铁肌红蛋白相对含量逐渐增加，导致肉色发生褐变。本研究结果有利于理解秦川牛肉贮藏过程肉类变色的复杂生化变化机制。     

The impacts of cattle access points on deposited sediment levels in headwater streams in Ireland

Cattle access to streams has been linked globally with degradation of stream water quality, driven largely by bank erosion and resultant instream, fine sediment deposition. The majority of evidence on such effects is however based in arid and semiarid regions of the United States and Australia, with few studies relating to cool temperate climates such as Northwest Europe. In this study, “Quorer” resuspendable sediment samples were taken from riffle geomorphic units upstream (control) and at two points downstream (pressure and recovery) of cattle access points in headwater streams in agricultural catchments in Ireland to assess levels of deposited stream sediment. Samples were taken in April/May (2016) prior to the grazing season and in October (2016) at the end of the grazing season. Sites in good‐high ecological status catchments and less than good ecological status catchments were included in the study. Higher levels of sediment were found downstream of cattle access points in both good‐high status and less than good status catchments; however, the impacts of access points were spatially confined to, in most cases, the area immediately downstream of the point of access. There was a strong correlation between deposited sediment mass and organic matter (OM) mass, with levels of OM increasing linearly with deposited sediment mass. Levels of measured sediment were negatively correlated with riparian habitat health (measured using a qualitative habitat assessment). The results of this study highlight the need for measures to prevent cattle access to headwater streams where access points can be many in order to manage local habitat quality and downstream water quality issues.     

关于解决黄牛鞋面革松面问题的探讨

本文讨论了在黄牛鞋面革生产过程中产生松面的原因，并经过多年的生产实践，摸索出解决松面问题行之有效的技术措施。     

The geographies of a more just food system: Building landscapes for social reproduction

Within the lifetime of a generation of people now involved in work in the cattle and beef industry in South Dakota, the landscapes of social reproduction that support and give shape to life for people there have been rearranged. Many of the social and physical relationships that developed to support smaller-scale, diverse farms and their surrounding communities are being made redundant by a new system of beef production and retailing. The costs of these redundancies are felt in dwindling small towns and an ever sparser rural population. Considering these changes as parts of landscapes of social reproduction is a means to connect the political and corporate logic driving our food systems to the spatial production of difference in the conditions for livelihood, as well as the spatial production of livelihood itself for both producers and consumers. Such an analysis is necessary in order to address the injustices inherent within our current food system.     

基于WSN的精准养牛业智能决策支持系统设计

准确及时地获取牛群的身体变化情况是保证牛群生存环境舒适度的基本前提。在对养牛业监测系统和无线传感网络深入了解的情况下,开发了以无线传感网络为基础的精准养牛业系统。按照标准生产化的要求,系统的总体架构分为感知层、传输层和应用层。并根据总体架构的层次模型设计了相应的软件和硬件。系统有效地利用贝叶斯网络智能决策支持系统建立模型,结合无线传感网络收集牛酮体的数据,进行相应的环境设置,让牛群的生存环境更加舒适。     

基于PDA的肉牛养殖可追溯系统的设计与实现

为提高肉牛养殖信息采集、传输的效率和准确性,本文通过对肉牛养殖企业的实地调研,在分析肉牛养殖流程的基础上,对肉牛养殖环节质量安全的关键信息进行了分析,并设计了基于PDA肉牛养殖质量安全可追溯系统的整体结构和功能模块,重点分析了该系统的实现技术。该系统改变了肉牛养殖纸质、人工的信息采集方式,实现了养殖场信息的快速、高效、准确的采集与传输。     

基于RFID技术的肉牛养殖质量安全可追溯系统研究

消费者和国际贸易对食品质量的要求日益提高,而运用监控、预警、追踪、追溯等技术在食品生产的关键环节上进行的质量监控是有效保障食品质量安全的重要手段。研究采用B/S（浏览器/服务器）模式结构体系,基于RFID技术构建了肉牛养殖可追溯系统。系统通过应用RFID技术,实现了对每头肉牛的养殖信息的自动采集,并针对每头肉牛形成可在Internet网络上进行查询的质量追溯档案。解决了牛肉在肉牛养殖环节（牛肉生产源头）上进行以肉牛个体为单位实施质量监控和实现可追溯的问题。     

Assessing genomic prediction accuracy for Holstein sires using bootstrap aggregation sampling and leave-one-out cross validation

Since the introduction of genome-enabled prediction for dairy cattle in 2009, genomic selection has markedly changed many aspects of the dairy genetics industry and enhanced the rate of response to selection for most economically important traits. Young dairy bulls are genotyped to obtain their genomic predicted transmitting ability (GPTA) and reliability (REL) values. These GPTA are a main factor in most purchasing, marketing, and culling decisions until bulls reach 5 yr of age and their milk-recorded offspring become available. At that time, daughter yield deviations (DYD) can be compared with the GPTA computed several years earlier. For most bulls, the DYD align well with the initial predictions. However, for some bulls, the difference between DYD and corresponding GPTA is quite large, and published REL are of limited value in identifying such bulls. A method of bootstrap aggregation sampling (bagging) using genomic BLUP (GBLUP) was applied to predict the GPTA of 2,963, 2,963, and 2,803 young Holstein bulls for protein yield, somatic cell score, and daughter pregnancy rate (DPR), respectively. For each trait, 50 bootstrap samples from a reference population comprising 2011 DYD of 8,610, 8,405, and 7,945 older Holstein bulls were used. Leave-one-out cross validation was also performed to assess prediction accuracy when removing specific bulls from the reference population. The main objectives of this study were (1) to assess the extent to which current REL values and alternative measures of variability, such as the bootstrap standard deviation (SD) of predictions, could detect bulls whose daughter performance deviates significantly from early genomic predictions, and (2) to identify factors associated with the reference population that inform about inaccurate genomic predictions. The SD of bootstrap predictions was a mildly useful metric for identifying bulls whose future daughter performance may deviate significantly from early GPTA for protein and DPR. Leave-one-out cross validation allowed us to identify groups of reference population bulls that were influential on other reference population bulls for protein yield and observe their effects on predictions of testing set bulls, as a whole and individually.