蜂王浆产品中10-羟基-2-癸烯酸的含量稳定性研究

10-羧基-2-癸烯酸(10-HDA)是蜂王浆特有的不饱和脂肪酸,在其质量标准中特别规定了其含量要求。采用纳米技术制备纳米脂质体可提高其稳定性,本文以包封率为评定指标,采用乙醇注入-超声法制备了10-HDA纳米脂质体,并用液相色谱测定其中的10-HDA含量,比较了五种蜂王浆产品中10-HDA的含量稳定性。结果发现,10-HDA纳米脂质体包封率可达98.3%,同时其含量最为稳定,而其它四种蜂王浆产品中10-HDA含量不稳定,随着时间的延长呈下降趋势。     

C-Shaped Cartilage Development Using Wharton's Jelly-Derived Hydrogels to Assemble a Highly Biomimetic Neotrachea for use in Circumferential Tracheal Reconstruction

A highly biomimetic neotrachea with C-shaped cartilage rings has promising clinical applications in the treatment of circumferential tracheal defects (CTDs) owing to its structure and physiological function. However, to date, most fabricated tracheal cartilages are O-shaped. In this study, finite element analysis demonstrates C-shaped cartilage rings that exhibit better compliance than O-shaped. Hydrogel is developed using methacryloyl-modified decellularized Wharton's jelly matrix (DWJMA) for the regeneration of C-shaped cartilage rings. This novel hydrogel possesses adjustable physicochemical properties and favorable cytocompatibility. When loaded with chondrocytes, DWJMA hydrogels support the optimal cartilage regeneration both in vitro and in vivo. More importantly, a highly biomimetic neotrachea simultaneously simulating the structural and physiological properties of the normal trachea is regenerated via modular assembly of several individual C-shaped cartilage rings. The results demonstrate the highly biomimetic neotrachea have better patency (88.6 ± 6.1% vs 74.4 ± 9.4%, p < 0.05), improve the survival rate, alleviate weight loss and mucoid impaction, than its O-shaped counterpart when used for the treatment of CTDs in a rabbit model. Therefore, this study proposes a novel hydrogel for the regeneration of C-shaped cartilage and provides new insights into the treatment of CTDs using a highly biomimetic neotrachea with C-shaped cartilage rings.     

草莓原果果冻的研制

采用草莓原果果浆制作果冻。目的是控制食品添加剂使用量，降低果冻弹性，避免因吸食果冻而造成的风险。同时由于果浆的应用，赋予产品丰富的营养。     

反相高效液相色谱法测定蜂王浆中的10-羟基-2-癸烯酸

采用反相高效液相色谱法测定蜂王浆中的10-羟基-2-癸烯酸.采用ZORBAX Eclipse XDB-C18柱,以甲醇:磷酸1%溶液(50:50, v/v)为流动相,流速为1.0 mL/min,检测波长210 nm.该法简便、快速、重现性好、回收率高.     

发用定型产品的功效评价

针对目前市场上琳琅满目的定型产品,消费者大多从产品的价格、品牌知名度、香型、使用的方便性及产品功效宣称等方面进行挑选,具有一定的盲目性.从产品的性能和定型效果两个方面,对市售10余个知名品牌的20余种啫喱水和啫喱膏的功效进行了评价.     

采用毛细管凝胶电泳技术检测蜂王浆新鲜度

新鲜度是评价蜂王浆产品质量一个重要指标。文中采用毛细管凝胶电泳技术对蜂王浆水溶性蛋白质进行了分离测定。结果表明:毛细管电泳技术对蜂王浆水溶性蛋白定量准确、重复性、精密度、稳定性各项指标良好,操作简便,快速,适合于蜂王浆产品的新鲜度的评价。为了筛选新鲜度指示蛋白,将王浆样品在不同温度下放置不同时间,分别进行毛细管电泳分析,最终从蜂王浆19个蛋白峰中筛选到2个蛋白峰,含量随存储温度和时间呈现规律性变化,可以作为蜂王浆新鲜度指示蛋白。     

橡子凉粉的研制

以橡子为原料,添加适量食品添加剂,可生产出一种新型方便食品—橡子凉粉。主要探讨了橡子凉粉的生产工艺参数和配方。     

春砂仁凝胶软糖的研制

采用广东阳春春砂仁的提取浓缩物为原料,选择并比较了几种亲水胶体的凝胶性能及形成凝胶软糖的工艺条件。通过胶体的复配,研制出一种外观晶莹透明、质地软糯而富有弹性、口感具有春砂仁特征滋味的凝胶软糖。其中亲水胶体含量为1%、总糖含量为66%、水分含量为17.6%。     

富含猕猴桃果水溶多糖的营养型保健果冻的研制

目的探讨富含猕猴桃果水溶性多糖的营养型保健果冻的制备。方法以猕猴桃汁、赤砂糖为主要原料,运用正交试验设计对猕猴桃果冻的加工工艺进行了研究。结果30%猕猴桃汁、15%糖(赤砂糖:白砂糖质量比=2:1),0.2%柠檬酸、6%凝胶剂(明胶:琼脂质量比=5:1)的工艺参数,可研制出色泽均匀、组织状态良好、口感细腻、酸甜适宜、香气协调的营养保健型果冻。结论该工艺合理、可行。