Effects of Quillaja saponaria extract and Nα-lauroyl-l-arginine ethyl ester on reducing selected foodborne pathogens in vitro and maintaining quality of fresh-cut endive (Cichorium endivia L.) at pilot plant scale

The aim of the present study was to evaluate the applicability of Quillaja saponaria extract (QSE) and N^α-lauroyl-l-arginine ethyl ester (LAE) as antimicrobial wash water additives in fresh-cut lettuce processing. Antibacterial activities of LAE and QSE against selected strains of the foodborne pathogens Salmonella enterica, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, and Listeria monocytogenes were examined in vitro. Minimum inhibitory concentrations determined by broth microdilution assay demonstrated that LAE exhibited a strong antimicrobial activity with MICs between 4 and 32 μg/mL against all tested strains, whereas QSE showed a weaker antimicrobial activity with MICs >512 μg/mL. On a pilot-plant scale, the effects of warm water washing at 45 °C for 120 s with and without 40 mg/L QSE or 100 mg/L LAE as well as cold water washing at 4 °C for 120 s with QSE or LAE, respectively, of shredded endive (Cichorium endivia L.) were investigated regarding microbiological and sensory quality as well as physiological properties. Samples were analyzed for headspace O₂ and CO₂ levels, phenylalanine-ammonia-lyase activity and contents of nitrite and nitrate during nine days of cold storage at 4 °C. By analogy to its antimicrobial effect against the foodborne pathogens in vitro, LAE allowed up to 4 log₁₀ cfu/mL reduction of the microbial load in the washing water of the pilot plant, and might therefore reduce cross-contamination while saving water. The addition of LAE to warm washing water impaired sensory properties of fresh-cut endive during storage, which was predicted by chlorophyll fluorescence imaging analyses. QSE treatment combined with warm water washing best retained sensory appearance throughout our study, being possibly suitable for the production of premium products.     

Combined effect of ultrasound and organic acids to reduce Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on organic fresh lettuce

This study was performed to compare the effectiveness of individual treatments (ultrasound and organic acids) and their combination on reducing foodborne pathogens on organic fresh lettuce. Lettuce leaves were inoculated with a cocktail of three strains each of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes and treated with ultrasound (40 kHz) alone, organic acids (0.3, 0.5, 0.7, 1.0, and 2.0% — malic acid, lactic acid, and citric acid) alone and combined with ultrasound and organic acids for 5 min. For all 3 pathogens, the combined treatment of ultrasound and organic acids resulted in additional 0.8 to 1.0 log reduction compared to individual treatments, without causing significant quality change (color and texture) on lettuce during 7 day storage. The maximum reductions of E. coli O157:H7, S. Typhimurium, and L. monocytogenes were 2.75, 3.18, and 2.87 log CFU/g observed after combined treatment with ultrasound and 2% organic acid for 5 min, respectively. Our results suggest that the combined treatment of ultrasound with organic acids was effective at increasing pathogen reduction compared to individual treatments without significantly affecting quality, and demonstrates its potential as a novel method to increase the microbial safety on organic fresh lettuce.     

Browning susceptibility of minimally processed Baby and Romaine lettuces

 This study was conducted to determine the effects of cultivar, tissue susceptibility and storage temperature on the keeping quality of minimally processed Baby and Romaine lettuces. Midrib and photosynthetic tissues were lightly processed and stored for up to 7 days at 5  °C and 13  °C. Changes in L ^* and a ^* values and absorbance at 430 nm were compared. The relationship between polyphenols, phenylalanine ammonia lyase (PAL) and polyphenol oxidase (PPO) activities and browning was examined. Measurements of L ^* and a ^* values on midribs showed that browning discoloration was the same for Baby as for Romaine lettuces and there was no relationship for temperature. In addition, L ^* and a ^* values in photosynthetic tissue indicated a decrease in green pigmentation during storage, especially at 13  °C. For both kinds of tissue at the end of cold storage an increase in soluble brown polymers was detected. In midribs, total phenolics increased significantly throughout the storage period because of the tissue-wounding response. The photosynthetic tissue had a higher phenolic content than the midrib one. For both kinds of tissue an increase in PPO activity occurred throughout cold storage. On the other hand, PAL activity in midrib tissue only increased initially, followed by a slow decline to reach normal levels. Browning potential estimated by L ^* values correlated significantly (P>95%) with PPO activity and with absorbance at 430 nm for Romaine lettuce. Based on colour and browning potential no differences between Romaine and Baby lettuce cultivars were observed. However, photosynthetic tissue was the most suitable tissue for the preparation of minimally processed salad mixes because of its high phenolic content. Received: 28 September 1998     

Uptake of arsenic species by turnip (Brassica rapa L.) and lettuce (Lactuca sativa L.) treated with roxarsone and its metabolites in chicken manure

Roxarsone is an organoarsenic feed additive that can be metabolised to other higher toxic arsenic (As) species in animal manure such as arsenate, arsenite, monomethylarsonic acid, dimethylarsinic acid, 3-amino-4-hydroxyphenylarsonic acid and other unknown As species. The accumulation, transport and distribution of As species in turnip (Brassica rapa L.) and lettuce (Lactuca sativa L.) amended with roxarsone and its metabolites in chicken manure were investigated. Results showed arsenite was the predominant As form, followed by arsenate in turnip and lettuce plants, and a low content of dimethylarsinic acid was detected only in lettuce roots. Compared with the control plants treated with chicken manure without roxarsone and its metabolites, the treatments containing roxarsone and its metabolites increased arsenite content by 2.0–3.2% in turnip shoots, by 6.6–6.7% in lettuce shoots, by 11–44% in turnip tubers and by 18–20% in lettuce roots at two growth stages. The enhanced proportion of arsenate content in turnip shoots, turnip tubers and lettuce roots was 4.3–14%, 20–35% and 70%, respectively, while dimethylarsinic acid content in lettuce roots increased 2.4 times. Results showed that the occurrence of dimethylarsinic acid in lettuce roots might be converted from the inorganic As species and the uptake of both inorganic and organic As compounds in turnip and lettuce plants would be enhanced by roxarsone and its metabolites in chicken manure. The pathway of roxarsone metabolites introduced into the human body via roxarsone → animal → manure → soil → crop was indicated.     

Effect of blanching on microwave freeze drying of stem lettuce cubes in a circular conduit drying chamber

Effects of boiling water and microwave blanching methods on the dielectric properties, electrical conductivity as well as microstructure of stem lettuce cubes (as a model for plant tissue) were studied. The study also focused on how different blanching methods affect microwave freeze-drying (MFD) in a circular conduit drying chamber and conventional freeze drying (FD) of stem lettuce cubes. Results showed that the electrical conductivity of samples blanched by microwave was two times higher than that of boiling water blanched ones and five times higher than that of unblanched samples. Apart from that, dielectric constant (ε′) and loss factor (ε″) of the stem lettuce cubes decreased significantly after freezing. MFD duration of microwave blanched samples was approximately 4.5 h, reduced by 30% compared to boiling water blanched ones. MFD process in a circular conduit yielded products of high quality compared to that in a tray.     

Browning on the surface of cut lettuce slices inhibited by short term exposure to nitric oxide (NO)

Freshly cut lettuce slices (Latuca sativa L.) were fumigated with nitric oxide (NO) gas at concentrations between 5 and 1000 μl/l in air at 20 °C for 1–4 h or dipped in an aqueous solution of the NO-donor compound, 2,2′-(hydroxynitrosohydrazino)-bisethanamine (DETANO) at concentrations between 10 and 1000 mg/l for 15 s to 60 min at 20 °C. Development of browning on the cut surfaces was inhibited during subsequent storage at 0 °C. The most effective treatments for extending postharvest life of lettuce slices were fumigation with 500 μl/l NO for 1 h, and dipping in 500 mg/l DETANO for 5 min. Dipping in DETANO solution was, however, more effective as it generated a 100% increase in postharvest life compared with a 70% increase due to NO gas. Solutions of DETANO in water were found to be relatively stable as the same extension in postharvest life was obtained for five batches of lettuce sequentially dipped in the same solution.     

Prediction Equations for Gases in Flexible Modified-Atmosphere Packages of Respiring Produce are Different Than Those for Rigid Packages

Total pressure generally decreases in a sealed rigid package containing respiring produce, whereas total pressure is essentially constant and free volume decreases in a flexible package. We found that predicted O₂, CO₂, and N₂ partial pressures were different for similarly designed (same surface area, thickness, film permeabilities and produce mass) flexible and rigid packages at “quasi steady state” and steady state, respectively. Predicted O₂ and CO₂ partial pressures were slightly higher in a flexible package than in a rigid package and were a function of the film permeability ratio of N₂ to O₂ and of CO₂ to O₂. They also related to the ratio of product CO₂ production rate to O₂ uptake rate, target steady-state O₂ partial pressure, and respiration characteristics. Differences were slight for films such as low-density polyethylene.     

莴笋泡菜护绿护脆工艺研究

实验对莴笋泡莱护绿护脆工艺进行研究,通过正交试验确定最佳工艺条件:护脆处理:低温漂烫时间20 min,温度55℃,乳酸钙添加量为20 g/L;护绿处理:铜离子浓度为500μg/mL,锌离子浓度为500μg/mL,温度为80℃,浸泡时间为3 h.     

清洗方式对鲜切莴笋品质和农残去除的影响

研究不同清洗方式对鲜切莴笋中的有机磷农药去除作用及对其品质指标的影响。结果表明:次氯酸钙和果蔬清洗机均能去除农药残留,保持鲜切莴笋的品质,其中果蔬清洗机清洗15min,能更有效的去除农药残留,抑制呼吸强度,杀灭微生物。     

包装薄膜对鲜切莴笋品质的影响

以普通塑料薄膜保鲜袋作对照,研究不同包装薄膜(0.03mm聚氯乙烯薄膜、0.03mm聚乙烯薄膜、0.03mm微孔薄膜)对鲜切莴笋贮藏期间品质的影响。结果表明:0.03mm厚聚乙烯薄膜包装能有效地控制鲜切莴笋的失重率、褐变度、呼吸强度、Vc损失率,提高鲜切莴笋的感官品质和营养价值。