The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study

Several decontamination methods for removing biofilm from implant surfaces during surgical peri-implantitis treatment have been reported, including the intraoperative usage of chlorhexidine (CHX)-based antiseptics. There is a lack of information on possible adverse effects on bone healing. The study aimed to examine the impact of three CHX-based mouthwashes on osteoblast-like cells (SaOS-2) in vitro. Cells were cultured for three days in 96-well binding plates. Each well was randomly treated for either 30, 60 or 120 s with 0.05% CHX combined with 0.05% cetylpyridinium chloride (CPC), 0.1% CHX, 0.2% CHX or sterile saline (NaCl) as control. Cell viability, cytotoxicity and apoptosis were assessed at day 0, 3 and 6. Cell viability resulted in being higher in the control group at all time points. At day 0, the CHX 0.2 group showed significantly higher cytotoxicity values compared to CHX 0.1 (30 s), CHX + CPC (30 s, 60 s and 120 s) and control (60 s and 120 s), while no significant differences were identified between CHX + CPC and both CHX 0.1 and NaCl groups. All test mouthwashes were found to induce apoptosis to a lower extent compared to control. Results indicate that 0.2% CHX presented the highest cytotoxic effect. Therefore, its intraoperative use should be carefully considered.     

高效液相色谱-串联质谱法同时测定漱口水中的11种磺胺类药物

目的 建立一种高效液相色谱-串联质谱法(high performance liquid chromatography tandem mass spectrometry, HPLC-MS/MS)同时测定漱口水中11种磺胺类药物(磺胺嘧啶、磺胺噻唑、磺胺吡啶、磺胺甲基嘧啶、磺胺甲噻二唑、磺胺氯哒嗪、磺胺甲基异噁唑、磺胺二甲异噁唑、磺胺喹噁啉、磺胺硝苯、磺胺苯吡唑)残留的方法。方法 样品经超声提取后, 以Agilent Eclipse XDB-C18(10 cm×4.6 mm, 3.5 μm)色谱柱分离后在高效液相色谱-串联质谱法多反应监测模式下进行测定, 外标法定量。结果 11种磺胺类药物的检出限为15 μg/kg, 在3个添加水平范围内的平均回收率为80.3%~105.4%, 相对标准偏差均小于8.5%。结论 该方法简便、准确, 快速, 适用于漱口水中磺胺类药物的测定。